Article
Plant Sciences
Hui Xu, Zhen Li, Peng-Fei Jiang, Li Zhao, Chang Qu, Yves Van de Peer, Yan-Jing Liu, Qing-Yin Zeng
Summary: Enzymes are essential for all living systems, and in plants, the glutaredoxin (GRX) family of enzymes plays important roles in development and stress tolerance. This study identified and classified 41 GRX genes in the poplar genome based on differences in gene structure, expression patterns, subcellular localization, enzymatic activity, and substrate specificity. Site-directed mutagenesis revealed that the divergence of active site motifs among different classes of GRX proteins led to substrate switches, providing new insights into the molecular evolution of these important plant enzymes.
Article
Biochemistry & Molecular Biology
Keita Kogure, Akiko Watanabe, Yukihiro Ito
Summary: This study identified ketoacyl CoA reductase (KCR) as an interacting partner of ONI1 and ONI2. The results suggest that ONI2 and KCR1 may form an FAE complex that plays a role in biosynthesizing very-long-chain fatty acids during shoot development in rice.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Wook Lee
Summary: The substrate specificity of beta-ketoacyl ACP synthase I (KasA) is highly linked to its cooperativity, which is realized through the activation of catalytic residues.
Article
Agronomy
Xiuying Gao, Ye Zhang, Hongsheng Zhang, Ji Huang
Summary: In this study, the researchers analyzed nine members of the 13-ketoacyl-CoA synthase gene family in rice and found that OsCUT1 is a promising gene for enhancing drought tolerance in rice. Overexpression of OsCUT1 significantly increased drought resistance without causing significant changes in major agronomic traits.
Article
Biochemistry & Molecular Biology
Shuangjuan Yang, Hao Tang, Xiaochun Wei, Yanyan Zhao, Zhiyong Wang, Henan Su, Liujing Niu, Yuxiang Yuan, Xiaowei Zhang
Summary: In this study, a new glossy mutant, SD369, was identified in Chinese cabbage. The mutant had significantly decreased wax monomers longer than 26 carbons. Inheritance analysis showed that the glossy trait of SD369 was controlled by a single recessive locus, BrWAX3. The candidate gene, BrCER60.A09, was identified and found to have a loss of function due to a premature stop codon caused by a 5567-bp insertion in exon 1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Haodong Huang, Xianpeng Yang, Minglu Zheng, Zexi Chen, Zhuo Yang, Pan Wu, Matthew A. Jenks, Guangchao Wang, Tao Feng, Li Liu, Pingfang Yang, Shiyou Lu, Huayan Zhao
Summary: 3-KETOACYL-COA SYNTHASE3 (KCS3) lacks canonical catalytic activity and negatively regulates cuticular wax synthesis by reducing the activities of KCS6s across diverse plant taxa from Arabidopsis to moss. The role of KCS3 in regulating KCS6 activity involves physical interactions between specific subunits of the fatty acid elongation complex and is essential for maintaining wax homeostasis. The KCS3-KCS6 module plays a highly conserved and important role in finely regulating wax synthesis across diverse plant taxa.
Article
Biochemistry & Molecular Biology
Hongbin Yang, Wanjun Mei, Haoliang Wan, Rangwei Xu, Yunjiang Cheng
Summary: Cuticular wax plays a protective role in fruit development and postharvest storage by reducing fruit water loss, resisting stress, and affecting glossiness. The KCS genes identified in Citrinae species are involved in the synthesis of cuticular wax, with expansion and purifying selection observed in wild and cultivated species. Furthermore, CsKCS2 and CsKCS11 in Citrus sinensis are crucial for cuticular wax accumulation during fruit ripening.
Article
Plant Sciences
Haodong Huang, Xianpeng Yang, Minglu Zheng, Shiyou Lu, Huayan Zhao
Summary: This study reveals the regulatory roles of KCS3 and KCS12 in cuticle biosynthesis. Mutations in KCS3 have a greater impact on wax production, while mutations in KCS12 exert more severe effects on cutin synthesis. KCS12 and KCS3 have additive effects on cuticle formation.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Plant Sciences
Fang Liu, Pandi Wang, Xiaojuan Xiong, Xinhua Zeng, Xiaobo Zhang, Gang Wu
Summary: Nervonic acid (NA) is a crucial fatty acid for brain development, attracting significant research interest. Market demand has encouraged the development of refined, NA-enriched plant oils for further studies. Plant seed oils offer a renewable and environmentally friendly source of NA, but face hindrances in industrial production.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Organic
Zhi Lin, Xudong Qu
Summary: This article reviews three recently reported polyketide synthases (PKSs) that demonstrate novel and representative biosynthetic pathways, providing new insights into the mechanistic and structural diversity of PKSs.
TETRAHEDRON LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Noraiza Suhaimi, Yoshiaki Maeda, Tomoko Yoshino, Tsuyoshi Tanaka
Summary: This study investigated the impact of inhibiting ketoacyl-ACP synthase (KAS) and ketoacyl-CoA synthase (KCS) on PUFA production in the diatom Fistulifera solaris. The inhibition of KAS led to an increase in EPA content without affecting cell growth, while the inhibition of KCS did not significantly impact PUFA content. These findings suggest that specific suppression of KAS function may be a promising strategy to enhance omega-3 PUFA production in F. solaris.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Plant Sciences
Lingwei Yang, Junchao Fang, Jingxin Wang, Suozhen Hui, Liang Zhou, Bo Xu, Yujuan Chen, Yuanyuan Zhang, Changkai Lai, Guiai Jiao, Zhonghua Sheng, Xiangjin Wei, Gaoneng Shao, Lihong Xie, Ling Wang, Ying Chen, Fengli Zhao, Shikai Hu, Peisong Hu, Shaoqing Tang
Summary: In this study, 22 OsKCS genes were identified in rice, which are unevenly distributed on nine chromosomes. The OsKCS gene family is divided into six subclasses, and many cis-acting elements related to plant growth, light, hormone, and stress response were enriched in their promoters. Gene duplication played a crucial role in the expansion of the OsKCS gene family, and most KCS genes showed constitutive expression. The study also revealed the different responses of KCS genes to exogenous cadmium stress in japonica and indica backgrounds and the potential role of KCS genes with higher expression in leaves and seeds under cadmium stress.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Forestry
Zhuowei Li, Shijie Ma, Huan Song, Zheng Yang, Cuizhu Zhao, David Taylor, Meng Zhang
Summary: Nervonic acid (24:1) is a major component in nerve and brain tissues with important applications in the food and pharmaceutical industries. The research uncovered the mechanisms behind nervonic acid biosynthesis in the seeds of the endangered tree species Malania oleifera, identifying MoKCS11's substrate preference for 11Z-eicosenoic acid over erucic acid.
Article
Horticulture
Hongbin Yang, Mingfei Zhang, Xin Li, Zhifeng Zhu, Rangwei Xu, Feng Zhu, Juan Xu, Xiuxin Deng, Yunjiang Cheng
Summary: Cuticular wax is a hydrophobic protective layer on the surface of citrus fruit, which prevents water loss and improves fruit appearance quality. This study found that CsKCS2 is highly expressed during fruit ripening and is involved in fruit cuticular wax synthesis in Citrus sinensis. The researchers identified CsERF003, CsMYB7, and CsMYB102 as upstream regulators of CsKCS2 and showed that they can increase cuticular wax content in leaves by upregulating the expression of wax-related genes. This research provides new insights into the transcriptional regulation of fruit cuticular wax synthesis and identifies important regulatory factors in Citrus sinensis fruit.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Zhuang Zuo, Xue Sun, Lina Cao, Shuang Zhang, Jiajie Yu, Xiuyue Xu, Zhiru Xu, Guanjun Liu, Chunpu Qu
Summary: Fructokinase (FRK) is the main enzyme responsible for phosphorylating fructose and playing a crucial role in catalyzing the irreversible reaction of free fructose phosphorylation. Through bioinformatics analysis, seven hypothetical FRK genes were identified in the Populus trichocarpa genome, with phylogenetic analysis revealing two subgroups within the PtFRK family genes. The study found significant tissue-specific expression patterns of PtFRK genes in response to different nitrogen concentrations and forms, laying the groundwork for understanding the molecular mechanisms of nitrogen regulation in cell wall development.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Plant Sciences
Rebecca A. Smith, Eliana Gonzales-Vigil, Steven D. Karlen, Ji-Young Park, Fachuang Lu, Curtis G. Wilkerson, Lacey Samuels, John Ralph, Shawn D. Mansfield
Article
Biochemistry & Molecular Biology
Eliana Gonzales-Vigil, Charles A. Hefer, Michelle E. von Loessl, Jonathan La Mantia, Shawn D. Mansfield
Article
Multidisciplinary Sciences
Yoichiro Watanabe, Rene Schneider, Sarah Barkwill, Eliana Gonzales-Vigil, Joseph L. Hill, A. Lacey Samuels, Staffan Persson, Shawn D. Mansfield
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Chemistry, Multidisciplinary
Aditya Bhalla, Namita Bansal, Sivakumar Pattathil, Muyang Li, Wei Shen, Chrislyn A. Particka, Steven D. Karlen, Thanaphong Phongpreecha, Rachel R. Semaan, Eliana Gonzales-Vigil, John Ralph, Shawn D. Mansfield, Shi-You Ding, David B. Hodge, Eric L. Hegg
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2018)
Article
Biochemistry & Molecular Biology
Yuki Matsuba, Thuong T. H. Nguyen, Krystle Wiegert, Vasiliki Falara, Eliana Gonzales-Vigil, Bryan Leong, Petra Schaefer, David Kudrna, Rod A. Wing, Anthony M. Bolger, Bjoern Usadel, Alain Tissier, Alisdair R. Fernie, Comelius S. Barry, Eran Pichersky
Article
Biochemistry & Molecular Biology
Matthew A. Bedewitz, Elsa Gongora-Castillo, Joseph B. Uebler, Eliana Gonzales-Vigil, Krystle E. Wiegert-Rininger, Kevin L. Childs, John P. Hamilton, Brieanne Vaillancourt, Yun-Soo Yeo, Joseph Chappell, Dean DellaPenna, A. Daniel Jones, C. Robin Buell, Cornelius S. Barry
Article
Plant Sciences
Jin-Ho Kang, Eliana Gonzales-Vigil, Yuki Matsuba, Eran Pichersky, Cornelius S. Barry
Article
Multidisciplinary Sciences
C. G. Wilkerson, S. D. Mansfield, F. Lu, S. Withers, J. -Y. Park, S. D. Karlen, E. Gonzales-Vigil, D. Padmakshan, F. Unda, J. Rencoret, J. Ralph
Article
Plant Sciences
Danyu Yao, Eliana Gonzales-Vigil, Shawn D. Mansfield
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Chemistry, Organic
Arvind S. Soni, Condarache M. Vacariu, Jeff Y. Chen, Martin E. Tanner
Article
Plant Sciences
Eliana Gonzales-Vigil, Michelle E. VonLoessl, Jeff Y. Chen, Sitong Li, Tegan M. Haslam, Ljerka Kunst, Shawn D. Mansfield
Summary: Research on the role of PtCER2-like genes in Populus trichocarpa has shown that these genes play a significant role in cuticular wax biosynthesis, with PtCER2-like5 having a notable effect on the production of C28 fatty acids.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Richard S. Ayikpoe, Lingyang Zhu, Jeff Y. Chen, Chi P. Ting, Wilfred A. van der Donk
Summary: The domain of unknown function 692 (DUF692) is a family of enzymes involved in the biosynthesis of RiPP natural products. This study identified a new member of the DUF692 family, ChrH, and demonstrated its catalyzation of a unique chemical transformation. The characterization of ChrH expands the repertoire of reactions catalyzed by DUF692 enzymes.
ACS CENTRAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Shengfei Zhou, Troy Runge, Steven D. Karlen, John Ralph, Eliana Gonzales-Vigil, Shawn D. Mansfield