Article
Biochemistry & Molecular Biology
Sheron Hakopian, Dimitri Niks, Russ Hille
Summary: Superoxide generated in the reaction of reduced formate dehydrogenase FdsDABG from Cupriavidus necator with O-2 is found to be responsible for the loss of activity. Catalytic amounts of superoxide dismutase are found to protect FdsDABG just as well as more generally used stabilizing inhibitors such as nitrate.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Plant Sciences
Fatemeh Najafi, Gholamreza Kavoosi, Roghayeh Siahbalaei, Amina Kariminia
Summary: The study demonstrated that Agastache foeniculum essential oil and oil fraction have inhibitory effects on oxidative stress induced by hyperglycemia and inflammation, mainly by regulating the activity and expression levels of relevant enzymes to reduce the production of oxidative stress. At the molecular level, the components of AFEO and AFoil were found to interact with corresponding proteins through molecular docking, showing antioxidant and anti-diabetic effects.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sheng-Ling Chen, Ting-Shan Liu, Wei-Guo Zhang, Jian-Zhong Xu
Summary: By re-creating the NADPH and ATP biosynthetic pathways in Pichia pastoris, the production of alpha-farnesene was increased. Overexpressing ZWF1 and SOL3 improved NADPH supply, while inactivating PGI did not. Low intensity expression of cPOS5 aided in the production of alpha-farnesene. Finally, ATP production was increased by overexpressing APRT and inactivating GPD1. The resultant strain produced 41.7% more alpha-farnesene than the parent strain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Margarida Duarte, Cleide Ferreira, Gurleen Kaur Khandpur, Tamara Flohr, Jannik Zimmermann, Helena Castro, Johannes M. Herrmann, Bruce Morgan, Ana M. Tomas
Summary: The study shows that type II NADH dehydrogenase is essential in Leishmania parasites, even in the presence of complex I, while complex I can be dispensable in both species. This suggests unexplored functions for NDH2 and validates it as a candidate target for drugs against Leishmania parasites.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Julio Berrios, Chrispian W. Theron, Sebastien Steels, Belen Ponce, Edgar Velastegui, Cristina Bustos, Claudia Altamirano, Patrick Fickers
Summary: Komagataella phaffii is a yeast capable of utilizing methanol as the sole carbon and energy source. This study investigated the metabolic impact of disrupting the formaldehyde dissimilative pathway in the yeast. The results showed that the mutant strain lacking formaldehyde dehydrogenase activity had only a slight decrease in growth rate but exhibited higher sensitivity to formaldehyde pulses. Flux analysis revealed a high metabolic flexibility in response to the disrupted pathway.
Article
Food Science & Technology
Naila Boby, Eon-Bee Lee, Muhammad Aleem Abbas, Na-Hye Park, Sam-Pin Lee, Md. Sekendar Ali, Seung-Jin Lee, Seung-Chun Park
Summary: FSC pretreatment reduces blood alcohol and acetaldehyde concentrations, modulates liver enzyme activities, increases antioxidant enzyme expression, and decreases inflammatory cytokine gene expression in the liver. Additionally, it shows no acute oral toxicity in female rats and may be beneficial for reducing alcohol-induced damage.
Review
Biotechnology & Applied Microbiology
Giannina Espina, Joaquin Atalah, Jenny M. Blamey
Summary: The demand for enzymes in industrial processes is increasing globally, with extremophilic enzymes showing higher stability and activity, leading to diverse potential applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Trishna Dey, Pundi N. Rangarajan
Summary: In this study, it was found that the industrial yeast Pichia pastoris activates a post-transcriptional regulatory circuit during carbon starvation to promote the synthesis of key enzymes GDH2 and PEPCK involved in amino acid catabolism. Autophagy or an autophagy-related process plays a crucial role in this regulatory circuit, with carbon-starved Dgdh2 and Dpepck showing poor survival rates. This research highlights the essential role of amino acid catabolism during carbon starvation, a phenomenon not previously reported in other yeast species.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Khadijah A. Altammar, Jonathan Guyang Ling, Hussein M. Al-Bajalan, Iuan-Sheau Chin, Mukram Mohamed Mackeen, Nor Muhammad Mahadi, Abdul Munir Abdul Murad, Farah Diba Abu Bakar
Summary: Cutinases are hydrolytic enzymes that can degrade plant cutin. A new cutinase homolog, AnCUT3, has been identified and characterized in this study. The recombinant AnCUT3 exhibited optimal temperature and pH, as well as high catalytic efficiency. It also showed potential for degrading and modifying the surface of certain polymers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Food Science & Technology
Xue Zhang, Chunyue Zhang, Mian Zhou, Quanming Xia, Liqiang Fan, Liming Zhao
Summary: Chitin and its derivatives have diverse applications, and this research achieved increased chitin content in yeast cell walls through gene overexpression and culture condition optimization. This is the first report of chitin production in engineered Pichia pastoris via biosynthetic pathway enhancement.
Article
Pharmacology & Pharmacy
Can Liang, Yongbing Xu, Minxia Fan, Felix Wambua Muema, Guilin Chen, Mingquan Guo, Guangwan Hu
Summary: This study revealed the remarkable antioxidant and anti-hyperuricemia activities of Rodgersia podophylla and identified potential ligands for SOD and XOD using UF-LC-MS method.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Engineering, Environmental
Minjie Gao, Yuchen Zhou, Jiajun Yan, Li Zhu, Zhitao Li, Xiuyu Hu, Xiaobei Zhan
Summary: Rhodium is widely used in catalysis, but its adsorption by microorganisms is not well studied. In this work, waste P. pastoris and recombinant P. pastoris GS-R were used as novel adsorbents for Rh(III) adsorption, showing high adsorption capacity and selectivity. The study developed an environmentally-friendly strategy for recovering Rh(III) and other precious metals using P. pastoris.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Immunology
Nicholas J. MacDonald, Kavita Singh, Karine Reiter, Nguyen Vu, Richard Shimp, Apostolos G. Gittis, Beth Chen, Martin Burkhardt, Baoshan Zhang, Zhixiong Wang, Raul Herrera, Mackenzie Moler, Duck-Yeon Lee, Sachy Orr-Gonzalez, Jessica Herrod, Lynn E. Lambert, Kelly M. Rausch, Olga Muratova, David S. Jones, Yimin Wu, Albert J. Jin, David N. Garboczi, Patrick E. Duffy, David L. Narum
Summary: The development of a malaria vaccine that can block transmission of the parasite from mosquitoes to humans is crucial for malaria elimination efforts. The use of Pfs25, a protein on the surface of the parasite, is being investigated as a transmission-blocking vaccine. Two recombinant forms of Pfs25, Pfs25H and Pfs25M, have been studied for their structural and immunological differences.
Article
Fisheries
Zixuan Wu, Yujie Liu, Jiaqi Zheng, Yongzhao Zhou, Kefan Xing, Yuying Sun, Jiquan Zhang
Summary: Arginine kinase is crucial for organisms with high energy demands, and in Exopalaemon carinicauda, it may play a similar role as in other crustaceans. Its expression is predominantly in muscle and can change in response to bacterial challenges. Recombinant expression and purification of EcAK showed similar enzymatic characteristics to AK from Euphausia superba.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yu Chen, Jiafu Shi, Yizhou Wu, Zheyuan Guo, Shihao Li, Wenping Li, Zhenhua Wu, Hongjian Wang, Haifei Jiang, Zhongyi Jiang
Summary: Photosynthesis can be used for the recycling of NADH in bio-redox reactions. By designing the oxidation of biomass derivatives as an electron supply module and using SOD/CAT cascade catalysis as a ROS elimination module, the NADH degradation can be inhibited, leading to an increase in NADH yield and reaction rate. This NADH photosynthesis system provides a novel and efficient platform for biomass-to-chemical conversion.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Agriculture, Multidisciplinary
Adnan Cavka, Stefan Stagge, Leif J. Jonsson
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2015)
Article
Biotechnology & Applied Microbiology
Stefan Stagge, Adnan Cavka, Leif J. Jonsson
Article
Biotechnology & Applied Microbiology
S. Steinsiek, S. Frixel, S. Stagge, K. Bettenbrock
JOURNAL OF BIOTECHNOLOGY
(2011)
Article
Multidisciplinary Sciences
Poonam Sharma, Stefan Stagge, Martijn Bekker, Katja Bettenbrock, Klaas J. Hellingwerf
Article
Multidisciplinary Sciences
Sonja Steinsiek, Stefan Stagge, Katja Bettenbrock
Article
Multidisciplinary Sciences
Sebastian G. Henkel, Alexander Ter Beek, Sonja Steinsiek, Stefan Stagge, Katja Bettenbrock, M. Joost Teixeira de Mattos, Thomas Sauter, Oliver Sawodny, Michael Ederer
Article
Biochemistry & Molecular Biology
Matthew D. Rolfe, Andrea Ocone, Melanie R. Stapleton, Simon Hall, Eleanor W. Trotter, Robert K. Poole, Guido Sanguinetti, Jeffrey Green
Article
Microbiology
Michael Ederer, Sonja Steinsiek, Stefan Stagge, Matthew D. Rolfe, Alexander Ter Beek, David Knies, M. Joost Teixeira de Mattos, Thomas Sauter, Jeffrey Green, Robert K. Poole, Katja Bettenbrock, Oliver Sawodny
FRONTIERS IN MICROBIOLOGY
(2014)
Article
Agronomy
Dimitrios Ilanidis, Stefan Stagge, Leif J. Jonsson, Carlos Martin
Summary: Biochemical conversion of wheat straw was studied using hydrothermal pretreatment, enzymatic saccharification, and microbial fermentation. Different pretreatment conditions had significant effects on chemical composition, enzymatic digestibility, carbohydrate composition, inhibitory byproducts, furfural, and fermentability. Xylose concentration in pretreatment liquids increased with temperature up to 190 degrees C and then decreased.
Article
Engineering, Chemical
Dimitrios Ilanidis, Stefan Stagge, Bjorn Alriksson, Leif J. Jonsson
Summary: Conditioning lignocellulosic hydrolysates with sulfur oxyanions, such as dithionite, is an effective method to improve fermentability by counteracting inhibitory by-products. Optimal conditions include treatment at pH 5.5 or 8.5, around 75°C, and using intermediate dithionite dosage.
Article
Agricultural Engineering
Sanjeet Mehariya, Martin Plohm, Piotr Jablonski, Stefan Stagge, Leif J. Jonsson, Christiane Funk
Summary: This study explores the use of biomass from four different Nordic microalgal species as a carbohydrate-rich feedstock for polyhydroxybutyrate (PHB) production. Thermochemical pre-treatment with hydrochloric acid or sulphuric acid significantly increased the sugar yield. The sugar-rich hydrolysate was used as a carbon source for PHB production by Halomonas halophila, and the highest PHB production was achieved with hydrolysate from Chlorococcum sp. grown on BG-11 medium.
BIORESOURCE TECHNOLOGY
(2023)