Review
Veterinary Sciences
Cristina Uruen, Carla Garcia, Lorenzo Fraile, Jan Tommassen, Jesus Arenas
Summary: Streptococcus suis is a zoonotic pathogen causing severe infections in pigs and humans, resulting in economic losses. The lack of effective vaccines has led to extensive antibiotic use, leading to the emergence of resistance. The genetic mechanisms of resistance in S. suis are diverse, involving target modification, antibiotic inactivation enzymes, and mutations in antibiotic targets. S. suis forms microcolonies on host tissues, which contribute to antibiotic tolerance and the rapid acquisition of multidrug resistance.
VETERINARY RESEARCH
(2022)
Article
Agriculture, Multidisciplinary
Qianwei Qu, Wenqiang Cui, Xingyu Huang, Zhenxin Zhu, Yue Dong, Zhongwei Yuan, Chunliu Dong, Yadan Zheng, Xueying Chen, Shuguang Yuan, Yanhua Li
Summary: Owing to the excessive use of antibiotics in various sectors, multidrug-resistant Streptococcus suis (S. suis) has become a serious public health concern. Polyphenolic compound gallic acid has shown potential in improving the efficacy of sulfonamide antibiotics against multidrug-resistant S. suis. Mechanistic analysis revealed that gallic acid disrupts the integrity and function of the bacteria's cytoplasmic membrane by depleting the proton motive force. Additionally, gallic acid was found to regulate the expression of dihydrofolate reductase, inhibiting tetrahydrofolate synthesis. In an animal infection model, gallic acid successfully restored the activity of sulfadiazine sodium without inducing drug resistance. These findings offer valuable insights into the threat of antibiotic resistance and propose a promising strategy to overcome this crisis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Public, Environmental & Occupational Health
Nguyen Thao Thi Nguyen, Yen Thi Hai Luu, Trung Duc Hoang, Huyen Xuan Nguyen, Tung Duy Dao, Vuong Nghia Bui, Gregory C. Gray
Summary: A study was conducted in Northern Vietnam to estimate the prevalence of Streptococcus suis on large swine farms. The results showed a lower than expected prevalence of S. suis, suggesting that recent efforts to centralize Vietnam's pork industry with better biosecurity measures may have been effective in reducing S. suis prevalence on these large farms.
Article
Biotechnology & Applied Microbiology
Haodan Zhu, Lixiao Han, Yanxiu Ni, Zhengyu Yu, Dandan Wang, Junming Zhou, Bin Li, Wei Zhang, Kongwang He
Summary: Nisin demonstrates potent antibacterial activity against Streptococcus suis, affecting cell membrane integrity and ultrastructure, inhibiting biofilm formation and degrading preformed biofilms. In vivo studies show nisin's potential as a novel antimicrobial agent for treating and preventing S. suis infection in animals.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Microbiology
Madalina Iulia Siteavu, Roxana Ionela Drugea, Elena Pitoiu, Emilia Ciobotaru-Pirvu
Summary: Antimicrobial resistance is an important health issue in human and veterinary medicine. This study monitored the antimicrobial resistance of three key bacteria in porcine respiratory disease. The results showed that these bacteria maintained high susceptibility to commonly used antimicrobial agents, but had high resistance rates to certain drugs.
Article
Microbiology
Jianping Wang, Kexin Qi, Xuemei Bai, Zongfu Wu, Weiming Kang, Pujun Liang, Han Zheng, Jianguo Xu
Summary: In this study, antibiotic resistance genes in Streptococcus suis strains from slaughtered pigs in China were detected and associated mobile genetic elements (MGEs) were investigated. The results revealed that the horizontal transfer of MGEs, especially IMEs and CIMEs, played a critical role in the evolution of S. suis and the transmission of antibiotic resistance genes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Multidisciplinary
Chaoyang Zhang, Lu Liu, Peng Zhang, Jingpo Cui, Xiaoxia Qin, Lichao Ma, Kun Han, Zhanhui Wang, Shaolin Wang, Shuangyang Ding, Zhangqi Shen
Summary: Antimicrobial resistance is a major global health threat. This study identified a new resistance gene, srpA, in Streptococcus suis, which mediates resistance to multiple antibiotics. Functional analysis revealed that srpA protects the ribosome and clarifies the mechanisms underlying resistance to ribosomal antibiotics.
Article
Microbiology
Anusak Kerdsin, Nichari Bamphensin, Kulsatri Sittichottumrong, Ratchadaporn Ungcharoen, Parichart Boueroy, Peechanika Chopjitt, Rujirat Hatrongjit, Marcelo Gottschalk, Nuchsupha Sunthamala
Summary: A multiplex PCR method can differentiate disease-associated and non-disease-associated pathotypes of Streptococcus suis. This method was evaluated on human and clinically healthy pig isolates in Thailand, and showed high accuracy for human strains but low accuracy for pig strains. Further validation is needed using more diverse strains from different geographic areas and origins of isolation.
Article
Microbiology
Jingjiao Ma, Mingqing Wu, Zhaofei Wang, Denghui Yang, Silu Hou, Yuqiang Cheng, Heng'an Wang, Yaxian Yan, Jianhe Sun
Summary: The order of infection of pathogens affects clinical manifestations and mortality rates in co-infection. The group infected with Streptococcus suis first exhibited more severe clinical signs, while the mortality rate was lower in mice infected with influenza virus following bacteria.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Esther Prados de la Torre, Antonio Rodriguez-Franco, Manuel J. Rodriguez-Ortega
Summary: This study compared the exoproteome fraction of different human clinical isolates of Streptococcus suis, revealing key proteins with antigenic potential and providing important insights for further research into the pathogenic mechanisms of this bacterium.
Article
Microbiology
Shujie Wang, Defu Zhang, Chenggang Jiang, Haijuan He, Chenchen Cui, Weitong Duan, Shouping Hu, Jun Wang, Xuehui Cai
Summary: S. suis causes disease in pigs and is increasingly implicated in human disease worldwide. This study sequenced the genomes of multidrug-resistant S. suis serotype 28 and 31 strains. Different strains displayed varying pathogenicity in mouse infection models and harbored different resistance genes.
Article
Biotechnology & Applied Microbiology
Y. Liu, H. Wang, J. Gao, Z. Wen, L. Peng
Summary: This study demonstrates that cryptotanshinone alters the secondary structure of suilysin, inhibiting its pore-forming activity and reducing the inflammatory response caused by S. suis infection, providing protection against bacterial infections.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Gratiela Gradisteanu Pircalabioru, Laura Ioana Popa, Luminita Marutescu, Irina Gheorghe, Marcela Popa, Ilda Czobor Barbu, Rodica Cristescu, Mariana-Carmen Chifiriuc
Summary: Decades of antibiotic misuse have led to a rise in antibiotic-resistant bacteria, causing a concerning increase in antimicrobial-resistant infections. Bacteriocins, small antimicrobial peptides produced by certain bacteria, show promise as alternatives to traditional antibiotics. The objective of the study was to explore the potential of bacteriocins as antimicrobial therapeutics for infections mainly caused by resistant pathogens.
Article
Infectious Diseases
Chun-Liu Dong, Rui-Xiang Che, Tong Wu, Qian-Wei Qu, Mo Chen, Si-Di Zheng, Xue-Hui Cai, Gang Wang, Yan-Hua Li
Summary: The aim of this study was to investigate the antimicrobial resistance profiles and genotypes of Streptococcus suis in Heilongjiang Province, China. A total of 29 S. suis were isolated from 332 samples collected from 6 pig farms. The results showed that serotypes 2, 4 and 9 were prevalent, and all the clinical isolates were resistant to at least two antibacterial drugs. The most resisted drugs were macrolides, and the least resisted drugs were fluoroquinolones. Resistant genes ermB and aph (3 ')-IIIa were highly distributed among the isolates, with the detection rates of 79.31% and 75.86%. The formation of biofilm could be observed in all the isolated S. suis, among which D-1, LL-1 and LL-3 strains formed stronger biofilm structure than other strains. The results indicate that S. suis in Heilongjiang Province presents a multi-drug resistance to commonly used antimicrobial drugs, which was caused by the same target gene, the dissemination of drug resistance genes, and bacterial biofilm.
Article
Veterinary Sciences
Carlos Neila-Ibanez, Jordi Casal, Isabel Hennig-Pauka, Norbert Stockhofe-Zurwieden, Marcelo Gottschalk, Lourdes Migura-Garcia, Lola Pailler-Garcia, Sebastian Napp
Summary: The economic assessment of swine production diseases caused by Streptococcus suis in Germany, the Netherlands, and Spain reveals that the costs per pig vary between countries due to different control measures and expenditures, with antimicrobial use, especially beta-lactams, still playing a crucial role in disease control in Spain and Germany.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Genevieve LeBel, Katy Vaillancourt, Philippe Bercier, Daniel Grenier
ARCHIVES OF MICROBIOLOGY
(2019)
Article
Dentistry, Oral Surgery & Medicine
Amel Ben Lagha, Elisoa Andrian, Daniel Grenier
MOLECULAR ORAL MICROBIOLOGY
(2019)
Article
Microbiology
Mariem Souissi, Jabrane Azelmat, Kamel Chaieb, Daniel Grenier
Article
Integrative & Complementary Medicine
Marie-Pier Veilleux, Daniel Grenier
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2019)
Article
Chemistry, Medicinal
Amel Ben Lagha, Genevieve LeBel, Daniel Grenier
PHYTOTHERAPY RESEARCH
(2020)
Article
Biotechnology & Applied Microbiology
Daniel Grenier, Eve Marcoux, Jabrane Azelmat, Amel Ben Lagha, Philippe Gauthier
Article
Multidisciplinary Sciences
Amel Ben Lagha, Jabrane Azelmat, Katy Vaillancourt, Daniel Grenier
Summary: This study characterized the anti-inflammatory properties of a polyphenolic cinnamon fraction, showing that it reduced IL-6, IL-8, and TNF-alpha secretion in a dose-dependent manner and may prevent NF-kappa B activation induced by LPS. The cinnamon fraction also reduced LPS binding to monocytes and may act as a natural PPAR-gamma ligand, suggesting therapeutic potential for the treatment of periodontal diseases.
Article
Multidisciplinary Sciences
Amel Ben Lagha, Genevieve Pellerin, Katy Vaillancourt, Daniel Grenier
Summary: Periodontal diseases, including gingivitis and periodontitis, are a global oral health problem caused by bacteria like Porphyromonas gingivalis. This study found that a phenolic extract from tart cherry juice can inhibit the growth and activities of P. gingivalis, and protect the integrity of the oral epithelial barrier.
Article
Dentistry, Oral Surgery & Medicine
Pierre-Olivier Miron, Amel Ben Lagha, Jabrane Azelmat, Anibal Diogenes, Juliana Nascimento Santos, Daniel Grenier
Summary: The study found that TNF- has detrimental effects on the viability, proliferation rate, and mineralization potential of stem cells of the apical papilla. Additionally, treating SCAP with TNF- reduced the secretion of periostin and increased the secretion of various cytokines and MMPs.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Katy Vaillancourt, Amel Ben Lagha, Daniel Grenier
Summary: Green tea extract and EGCG can reduce the cytotoxicity of the chemotherapeutic agent irinotecan towards oral epithelial cells, decrease intracellular ROS production, inhibit cell proliferation and migration, and suppress the secretion of cytokines and MMPs.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Amel Ben Lagha, Ying Yang, Harsh M. Trivedi, James G. Masters, Daniel Grenier
Summary: The study demonstrated that Dual Zinc plus Arginine formulations can protect the barrier integrity of gingival keratinocytes, promote cell proliferation, and enhance cell migration.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Amel Ben Lagha, Patricia Maquera Huacho, Daniel Grenier
Summary: The study demonstrated that a cocoa extract rich in epicatechin can effectively inhibit the growth, biofilm formation, adherence, and H2S production of F. nucleatum. Additionally, it showed anti-inflammatory properties, reduced IL-6 and IL-8 production, and enhanced epithelial barrier function, suggesting that it may have potential in preventing and treating periodontal diseases.
Article
Biotechnology & Applied Microbiology
Katy Vaillancourt, Genevieve LeBel, Nahuel Fittipaldi, Michel Frenette, Marcelo Gottschalk, Daniel Grenier
Summary: In this study, a new bacteriocin produced by Streptococcus pluranimalium 2N12 was characterized. This bacteriocin, called pluranimalicin 2N12, exhibited antibacterial activity against Streptococcus suis and showed a dual action of both antibacterial and anti-inflammatory effects.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Infectious Diseases
Jinpeng Li, Qingying Fan, Jing Zuo, Bingqian Xue, Xiaoling Zhang, Ying Wei, Liyun Sun, Daniel Grenier, Li Yi, Xiaogai Hou, Yang Wang
Summary: This study suggests that the combination of paeoniflorin and norfloxacin can effectively combat FQ-resistant Streptococcus suis. Paeoniflorin inhibits the LuxS/AI-2 quorum sensing system and down-regulates the transcription of the FQ efflux pump gene, enhancing the bactericidal effect of norfloxacin. Additionally, paeoniflorin inhibits biofilm formation, promoting the ability of norfloxacin to kill S. suis. In a mouse model, paeoniflorin in combination with norfloxacin demonstrates efficacy in treating S. suis infections.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Genevieve Pellerin, Laurent Bazinet, Daniel Grenier
Summary: The study found that removal of organic acids from cranberry juice through electrodialysis weakened its antibacterial effect against certain periodontal pathogens, but had varying effects on the adherence of these pathogens to oral epithelial cells. Additionally, deacidification of cranberry juice reduced its cytotoxicity for oral epithelial cells and attenuated its anti-inflammatory effect.
Article
Biochemistry & Molecular Biology
Giovanni Pacini, Bo Ahren
Summary: Tirzepatide stimulates both insulin secretion and glucose effectiveness, with stimulation of glucose effectiveness being the prominent process to reduce glucose.
Article
Biochemistry & Molecular Biology
Si -yu Wang, Yu-zhe Zhang, Xiao-han Liu, Xue-ci Guo, Xiao-fang Wang, Feng-tong Han, Yao Zhang, Chang -lin Wang
Summary: In the formalin pain test, the EM-2 analogs EM-2-Me, EM-2-Et, and EM-2-Bu showed significant analgesic effects with reduced tolerance and gastrointestinal side effects. These effects were mediated through central opioid mechanisms, with EM-2-Me possibly involving dynorphin A release and EM-2-Bu directly activating multiple opioid receptors.
