Review
Veterinary Sciences
Tong Zhou, Mingshu Wang, Anchun Cheng, Qiao Yang, Bin Tian, Ying Wu, Renyong Jia, Shun Chen, Mafeng Liu, Xin-Xin Zhao, Xuming Ou, Sai Mao, Di Sun, Shaqiu Zhang, Dekang Zhu, Juan Huang, Qun Gao, Yanling Yu, Ling Zhang
Summary: This article mainly describes the viral protein kinases and their mechanisms of regulating viral protein function through phosphorylation. The study of post-translational modification of viral proteins is of great significance for understanding viral infection mechanisms and developing antiviral treatment.
VETERINARY RESEARCH
(2022)
Review
Mathematical & Computational Biology
Shahin Ramazi, Javad Zahiri
Summary: Posttranslational modifications (PTMs) involve modifications to amino acid side chains in proteins after biosynthesis, affecting various aspects of protein functions. Disruptions in PTMs can lead to diseases, emphasizing the need for computational methods to predict PTMs. High-throughput experimental methods for PTM discovery are laborious, prompting the exploration of computational tools and databases to advance research in this area.
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2021)
Article
Agriculture, Multidisciplinary
Qibin Wu, Zhenxiang Li, Jingtao Yang, Fu Xu, Xueqin Fu, Liping Xu, Chuihuai You, Dongjiao Wang, Yachun Su, Youxiong Que
Summary: This study reports the first comprehensive analysis of protein lysine acetylation, 2-hydroxyisobutyrylation, and lysine lactylation in sugarcane. These post-translational modifications were found to be involved in energy metabolism and stress response. The results provide new insights into the molecular mechanisms of protein PTMs in sugarcane.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Cell Biology
Xingde Wang, Xingzhi Xu, Zhifeng Wang
Summary: In this review, the authors summarize the known information about the UFM1 enzymatic cascade and its biological functions, discuss the identified substrates, and explore the pathological role of UFMylation in human disease.
Article
Chemistry, Multidisciplinary
Buyan Pan, Marie Shimogawa, Jun Zhao, Elizabeth Rhoades, Anna Kashina, E. James Petersson
Summary: In this study, a facile method for introducing a mimic of arginylation modification into a protein of interest is presented. The effects of this mimicking modification on protein aggregation and neuroprotection are investigated.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Li Cao, Moshe Beiser, Joseph D. Koos, Margarita Orlova, Hader E. Elashal, Hendrik V. Schroder, A. James Link
Summary: Lasso peptides are a family of ribosomally synthesized and post-translationally modified peptides with a threaded structure. PIMT enzymes play a new role in the post-translational modification of lasso peptides, methylating and converting L-aspartate residues to provide rigidity to the lasso peptide ring.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Karlie R. Platz, Emma J. Rudisel, Katelynn V. Paluch, Taylor R. Laurin, Kristin E. Dittenhafer-Reed
Summary: The mitochondrial proteome undergoes various post-translational modifications, and this study focused on the effects of acetylation and phosphorylation on the function of mitochondrial RNA polymerase (POLRMT). While some modifications showed slight decrease in the binding ability of POLRMT, there were minimal biological impacts observed in terms of viability, mtDNA content, and mitochondrial transcript levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Mingyue Zheng, Guoxiang Jin, Zhongjun Zhou
Summary: Lamins, ancient intermediate filament proteins, are functionally regulated by post-translational modifications, contributing to various biological functions. Deregulation of lamins is associated with abnormal nuclear morphology and chromatin disorganization, leading to diseases. Understanding these modifications provides new insights into the molecular mechanisms underlying diseases and potential therapeutic strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemical Research Methods
Sajad Rashidi, Renu Tuteja, Reza Mansouri, Mohammad Ali-Hassanzadeh, Reza Shafiei, Esmaeel Ghani, Mohammadreza Karimazar, Paul Nguewa, Raul Manzano-Roman
Summary: Investigating post-translational modifications in parasites is crucial for understanding their regulatory roles in survival, infectivity, and pathogenicity, with different forms of PTMs potentially playing key roles in various aspects of parasite biology.
JOURNAL OF PROTEOMICS
(2021)
Article
Biochemical Research Methods
Dimitrios Tsikas
Summary: Lysine residues in proteins undergo various chemical modifications, including carbonylation by glyoxal (GO) and methylglyoxal (MGO). Malondialdehyde (MDA) is another carbonyl species formed enzymatically and nonenzymatically. These carbonyl species can occur in free forms or be adducted to proteins, particularly lysine residues. MDA is commonly used as a biomarker of lipid peroxidation, with plasma and serum being the most frequently analyzed samples. However, preanalytical factors, such as artificial MDA formation in lipid-rich samples, can greatly affect MDA concentrations.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Dan Wu, Tao Liu
Summary: Understanding post-translational modifications is crucial for manipulating physiological processes in eukaryotes. Genetic code expansion technology has been used to investigate the roles of these modifications, and can be combined with synthetic biology to create genetically modified organisms. This article discusses the applications, limitations, and future perspectives of genetic code expansion technology for studying post-translational modifications, as well as the implications for genetically modified organisms.
Article
Biochemistry & Molecular Biology
Sharon L. Campbell, Mark R. Philips
Summary: Mutations in RAS genes are the most common driving force behind cancer development. RAS proteins, acting as binary molecular switches, control cellular growth through a complex signaling pathway. In addition to nucleotide-binding properties, RAS proteins are also regulated by numerous post-translational modifications, which are currently a high priority for drug discovery research in the field of RAS biology.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Article
Cell Biology
Jing-Hua Yang, Han-Pil Choi, Annie Yang, Roya Azad, Fengmei Chen, Zhangsuo Liu, Kazem M. Azadzoi
Summary: A comparison of protein sequence variations between ischemic and normal bladder tissues revealed a large number of non-coded amino acid residues in bladder ischemia, potentially serving as a novel biomarker for smooth muscle dysfunction.
Review
Oncology
Ana Gonzalez-Garcia, Antonio Garrido, Ana C. Carrera
Summary: Genetic alterations in the PI3-kinase/PTEN pathway are common in cancer cells. Current efforts to treat PTEN-dependent tumors mainly focus on PI3-kinase inhibition, but modulating PTEN post-translational modifications could provide alternative therapeutic strategies.
Article
Chemistry, Multidisciplinary
Julia Kraxner, Charlotta Lorenz, Julia Menzel, Iwan Parfentev, Ivan Silbern, Manuela Denz, Henning Urlaub, Blanche Schwappach, Sarah Koester
Summary: The mechanical properties of biological cells are influenced by the cytoskeleton, with phosphorylation serving as a fast mechanism for mechanical modulation. Phosphorylation can soften filaments, and the binding of 14-3-3 protein to phosphorylated filaments further enhances this effect, potentially preserving the softening and altering cell mechanics in the cell.
Review
Biochemistry & Molecular Biology
Martin J. Stone, Jenni A. Hayward, Cheng Huang, Zil E. Huma, Julie Sanchez
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Biochemical Research Methods
Cheng Huang, Caitlin Lewis, Natalie A. Borg, Meritxell Canals, Henry Diep, Grant R. Drummond, Robert J. Goode, Ralf B. Schittenhelm, Antony Vinh, Mingyu Zhu, Barbara Kemp-Harper, Oded Kleifeld, Martin J. Stone
JOURNAL OF PROTEOME RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Julie Sanchez, Zil E. Huma, J. Robert Lane, Xuyu Liu, Jessica L. Bridgford, Richard J. Payne, Meritxell Canals, Martin J. Stone
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Correction
Biochemistry & Molecular Biology
Jenni Hayward, Julie Sanchez, Andrew Perry, Cheng Huang, Manuel Rodriguez Valle, Meritxell Canals, Richard J. Payne, Martin J. Stone
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Julie Sanchez, J. Robert Lane, Meritxell Canals, Martin J. Stone
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Review
Biochemistry & Molecular Biology
Ram Prasad Bhusal, Simon R. Foster, Martin J. Stone
Review
Biochemistry & Molecular Biology
Ram Prasad Bhusal, James R. O. Eaton, Sayeeda T. Chowdhury, Christine A. Power, Amanda E. I. Proudfoot, Martin J. Stone, Shoumo Bhattacharya
TRENDS IN BIOCHEMICAL SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Cheng Huang, Simon R. Foster, Anup D. Shah, Oded Kleifeld, Meritxell Canals, Ralf B. Schittenhelm, Martin J. Stone
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Jason Johansen-Leete, Toby Passioura, Simon R. Foster, Ram Prasad Bhusal, Daniel J. Ford, Minglong Liu, Seino A. K. Jongkees, Hiroaki Suga, Martin J. Stone, Richard J. Payne
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Multidisciplinary Sciences
Charlotte Franck, Simon R. Foster, Jason Johansen-Leete, Sayeeda Chowdhury, Michelle Cielesh, Ram Prasad Bhusal, Joel P. Mackay, Mark Larance, Martin J. Stone, Richard J. Payne
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Herman D. Lim, J. Robert Lane, Meritxell Canals, Martin J. Stone
Summary: Chemokines interact with chemokine receptors in a promiscuous network, with each receptor being activated by multiple chemokines. Different chemokines can preferentially activate different signaling pathways via the same receptor, a phenomenon known as biased agonism. The study shows that different chemokine receptors exhibit varying signaling outcomes, suggesting that their intrinsic abilities to couple with downstream signaling pathways play a role in differential agonism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Health Care Sciences & Services
Vivienne Mak, Sunanthiny Krishnan, Sara Chuang
Summary: Objective Structured Clinical Examinations (OSCEs) are commonly used in healthcare education. This study evaluated second-year pharmacy students' and examiners' experiences of their first virtual OSCEs in Australia using a mixed-method design. The majority of students preferred the virtual experience, but face-to-face OSCEs were still considered important and irreplaceable.
Article
Education, Scientific Disciplines
Sunanthiny Krishnan, Sara Chuang, Vivienne Mak
Summary: The OSCE is a crucial component of pharmacy undergraduate programmes, and strategies need to be implemented to ensure academic integrity in online assessments.
PHARMACY EDUCATION
(2022)
Article
Pharmacology & Pharmacy
Sara Chuang, Kate Lorraine Grieve, Vivienne Mak
Summary: Pharmacists play a crucial role in medication supply, but dispensing errors can lead to patient harm and even death. Students often make errors in dispensing, particularly in label directions. It is necessary for pharmacy students to receive more comprehensive guidance and practice in safe environments to reduce dispensing errors.
Article
Education, Scientific Disciplines
Daniel T. Malone, Sara Chuang, Elizabeth Yuriev, Jennifer L. Short
Summary: Allowing final-year Bachelor of Pharmacy students to use a medicines formulary during examinations led to improved performance in higher levels of Bloom's taxonomy questions and a preference for open book examinations among students.
AMERICAN JOURNAL OF PHARMACEUTICAL EDUCATION
(2021)