Article
Oncology
Yi-Jie Lu, Yi-Ting Deng, Hui-Hsin Ko, Hsin-Hui Peng, Hsiang-Chieh Lee, Mark Yen-Ping Kuo, Shih-Jung Cheng
Summary: LOXL2 expression is significantly associated with clinical stage, lymph node metastasis, and overall survival time in oral squamous cell carcinoma (OSCC). Overexpression of LOXL2 enhances migration, invasion, epithelial-mesenchymal transition (EMT), and cancer stem cell (CSC) phenotypes, independent of its enzymatic activity. LOXL2 also increases the levels of IFIT1 and IFIT3, which are key downstream components of LOXL2 action in migration, invasion, EMT, and CSC phenotypes.
Article
Biochemistry & Molecular Biology
Kuo-Chu Lai, Prabha Regmi, Chung-Ji Liu, Jeng-Fan Lo, Te-Chang Lee
Summary: The study demonstrates that IFIT2 depletion promotes the CSC-like phenotypes in oral cancer cells. Clinically, there is a positive correlation between IFIT2 and CD24 in oral cancer patients, and the expression pattern of IFIT2(high)/CD24(high)/CD44(low) predicts a better prognosis. Furthermore, TNF-alpha blockade can abolish the sphere formation induced by IFIT2 depletion, suggesting its involvement in CSC-like phenotypes in oral cancer.
Article
Oncology
Tian-Hao Li, Bang-Bo Zhao, Cheng Qin, Yuan-Yang Wang, Ze-Ru Li, Hong-Tao Cao, Xiao-Ying Yang, Xing-Tong Zhou, Wei-Bin Wang
Summary: The study identified widespread expression of IFIT1 in pancreatic cancer, and found that it promotes proliferation, migration, and invasion of pancreatic cancer cells by activating the Wnt/β-catenin pathway and regulating epithelial-mesenchymal transition (EMT). IFIT1 may serve as a potential therapeutic target for pancreatic cancer.
Article
Infectious Diseases
Wenbin Qiao, Jiahui Fan, Xiaoqian Shang, Liang Wang, Bahetibieke Tuohetaerbaike, Ying Li, Li Zhang, YiShan Huo, Jing Wang, Xiumin Ma
Summary: Through bioinformatics analysis, we identified IFIT1 as an important substance involved in central nervous system tuberculosis, with significantly higher expression in the lesion tissue.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Virology
Craig Schindewolf, Kumari Lokugamage, Michelle N. Vu, Bryan A. Johnson, Dionna Scharton, Jessica A. Plante, Birte Kalveram, Patricia A. Crocquet-Valdes, Stephanea Sotcheff, Elizabeth Jaworski, Rojelio E. Alvarado, Kari Debbink, Matthew D. Daugherty, Scott C. Weaver, Andrew L. Routh, David H. Walker, Kenneth S. Plante, Vineet D. Menachery
Summary: Understanding the role of NSP16 in SARS-CoV-2 infection and pathogenesis, this study demonstrates the attenuation of a NSP16 mutant both in vitro and in vivo, as well as its sensitivity to IFN-I. Silencing IFIT1 or IFIT3 partially restores the mutant's fitness. Furthermore, targeting NSP16 with a MTase inhibitor enhances the virus' sensitivity to IFN-I. Overall, these findings highlight the importance of NSP16 in immune evasion and suggest a potential target for antiviral development.
JOURNAL OF VIROLOGY
(2023)
Article
Oceanography
Wang Lei, Ma Teng, Xu Wenteng, Chen Zhangfan, Zhou Qian, Zheng Guiliang, Chen Songlin
Summary: ELIFIT1 gene plays an important antiviral role in Epinephelus lanceolatus, with high expression in blood and immunity-related tissues. Upon viral infection, its expression is significantly upregulated and reaches peak levels within a specific timeframe.
JOURNAL OF OCEAN UNIVERSITY OF CHINA
(2021)
Article
Medicine, Research & Experimental
Bing-fen Yang, Fei Zhai, Shan Yu, Hong-juan An, Zhi-hong Cao, Yan-hua Liu, Ruo Wang, Xiao-xing Cheng
Summary: This study identified potential biomarkers for discriminating active tuberculosis (TB) from latent TB infection by transcriptome sequencing. IFIT3 and ORM1 showed high diagnostic value in distinguishing patients with active TB from individuals with latent TB infection, providing a new method for TB diagnosis.
CURRENT MEDICAL SCIENCE
(2022)
Article
Pediatrics
Hongai Li, Teng Wang, Bangtao Li, Ting Huang, Yuanping Hai, Chuican Huang, Wei Xiang
Summary: This study found that the expression of the IFIT1 gene is enhanced in childhood systemic lupus erythematosus (SLE) patients. IFIT1 and its affected differentially expressed genes (DEGs) have the potential to serve as diagnostic markers for childhood SLE and are associated with immune infiltration. This study elucidated that IFIT1-induced changes in the transcriptome are involved in immune checkpoint and tertiary lymphoid structure in childhood SLE.
TRANSLATIONAL PEDIATRICS
(2023)
Article
Hematology
Jian Zhang, Xiangkai Zhao, Yunyun Guo, Zhiping Liu, Shujian Wei, Qiuhuan Yuan, Haixia Shang, Wentao Sang, Sumei Cui, Tonghui Xu, Kehui Yang, Jialin Guo, Chang Pan, Jiali Wang, Jiaojiao Pang, Tianrui Han, Yuguo Chen, Feng Xu
Summary: The ALDH2 rs671 mutation is a risk factor for the development and prognosis of atherosclerotic cardiovascular diseases. This study uncovers the important role of the ALDH2-Rac2 axis in efferocytosis during atherosclerosis, providing a potential direction for therapeutic strategies in cardiovascular diseases.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mathias Jensen, Nicoline W. Thorsen, Line A. E. Hallberg, Per Hagglund, Clare L. Hawkins
Summary: Neutrophil extracellular trap (NET) release plays a key role in chronic diseases like atherosclerosis, but the release of macrophage extracellular traps (METs) and their composition are less understood. This study investigated MET release from human THP-1 macrophages exposed to different stimuli and discovered that METs were composed of histones, various proteins involved in different cellular processes, and the absence of proteases. These findings provide new insights into the implications of MET formation in immune defense and pathology.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Xing Fei Tan, Qifan Chen, San Hue Hua, George Wai Yip
Summary: The IFIT family of proteins is crucial in the antiviral immune response, with recent research highlighting their significance in cancer. They have the potential to serve as cancer biomarkers and prognostic factors in various types of malignant neoplasms.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Aarthi Subramani, Maria E. L. Hite, Sarah Garcia, Jack Maxwell, Hursha Kondee, Grace E. Millican, Erin E. McClelland, Rebecca L. Seipelt-Thiemann, David E. Nelson
Summary: Macrophages are the first line of defense against microbial pathogens. Exposure to interferon-gamma (IFNγ) enhances antimicrobial and proinflammatory activity through increasing interferon-stimulated gene (ISG) expression. CITED1, a transcriptional coregulator, is shown to enhance the expression of certain ISGs and maintain proinflammatory gene expression during prolonged IFNγ exposure, in contrast to its closely related protein CITED2. These findings suggest an antagonistic relationship between CITED proteins in regulating macrophage inflammatory function.
JOURNAL OF CELL SCIENCE
(2023)
Review
Hematology
Natalia Eberhardt, Chiara Giannarelli
Summary: The development of innovative single-cell technologies has allowed for in-depth analysis of the transcriptomic and proteomic profiling of individual blood and tissue cells. Recent single-cell studies have revealed the cellular heterogeneity of atherosclerotic plaque tissue and provided a better understanding of the immune functional states in the context of atherosclerosis.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yu-Ting Hsieh, Tsung-Lin Tsai, Shen-Yan Huang, Jian-Wen Heng, Yu-Chia Huang, Pei-Yuan Tsai, Chia-Chun Tu, Tai-Ling Chao, Ya-Min Tsai, Pei-Ching Chang, Chien-Kuo Lee, Guann-Yi Yu, Sui-Yuan Chang, Ivan L. L. Dzhagalov, Chia-Lin Hsu
Summary: A growing body of evidence highlights the role of metabolic reprogramming in immune cells to combat infections. However, the regulation of metabolite transporters that support metabolic demands remains poorly understood. This study demonstrates that equilibrative nucleoside transporter 3 (ENT3) is part of the innate immune response and is upregulated upon pathogen invasion. The expression of ENT3 is directly regulated by type I interferon-induced signaling and is required for the entry and replication of several viruses, including SARS-CoV-2.
Article
Microbiology
Ethan Iverson, Kira Griswold, Daniel Song, Talita B. Gagliardi, Kajal Hamidzadeh, Mehmet Kesimer, Sanju Sinha, Melissa Perry, Gregg A. Duncan, Margaret A. Scull
Summary: This study demonstrates the inhibitory role of MUC1 protein in the uptake and infection of IAV in human airway epithelium (HAE). MUC1 expression is increased through interferon and other soluble factors during IAV infection, and its genetic knockout enhances viral growth.
Article
Biology
Eric Mandela, Christopher J. Stubenrauch, David Ryoo, Hyea Hwang, Eli J. Cohen, Von L. Torres, Pankaj Deo, Chaille T. Webb, Cheng Huang, Ralf B. Schittenhelm, Morgan Beeby, J. C. Gumbart, Trevor Lithgow, Iain D. Hay
Summary: The cell envelope of Gram-negative bacteria is composed of two membranes and plays a crucial role in spatial constraints and load-bearing forces. This study focuses on the mechanism behind spatial constraints in Escherichia coli, specifically the role of the coiled-coil lipoprotein Lpp. The findings demonstrate that E. coli can accommodate elongated Lpp through various factors and genetic adaptations, highlighting the importance of maintaining spatial constraints in bacterial cell envelopes.
Article
Biochemistry & Molecular Biology
Ben B. Wang, Haijin Xu, Sandra Isenmann, Cheng Huang, Xabier Elorza-Vidal, Grigori Y. Rychkov, Raul Estevez, Ralf B. Schittenhelm, Gergely L. Lukacs, Pirjo M. Apaja
Summary: This study identifies Ubr1 as a protein quality control E3 ubiquitin-ligase that counteracts proteostasis stresses by facilitating endosomal cargo-selective autophagy. Ubr1 plays a critical role in endo-lysosomal proteostasis perturbations and is linked to Ca2+ homeostasis and diseases such as cancers and brain disorders.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Anna Harutyunyan, Debbie Chong, Rui Li, Anup D. Shah, Zahra Ali, Cheng Huang, Christopher K. Barlow, Piero Perucca, Terence J. O'Brien, Nigel C. Jones, Ralf B. Schittenhelm, Alison Anderson, Pablo M. Casillas-Espinosa
Summary: This study employed a multi-omic, network-based approach to characterize the molecular signature associated with absence epilepsy in a rat model. The findings revealed dysregulated pathways and central regulatory hubs in the seizure-associated module, suggesting their potential involvement in the etiology and maintenance of absence seizure activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Pranali Deore, Christopher K. Barlow, Ralf B. Schittenhelm, John Beardall, Santosh Noronha
Summary: This study used an untargeted approach to profile the temporal dynamics of Dunaliella tertiolecta-specific exo-metabolites under grazing pressure. The study identified potential markers of grazing-mediated stress and outlined a clear methodology for screening exo-metabolites in marine algal samples.
JOURNAL OF PHYCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xue Yang, Miguel I. Cruz, Elizabeth V. Nguyen, Cheng Huang, Ralf B. Schittenhelm, Jennii Luu, Karla J. Cowley, Sung-Young Shin, Lan K. Nguyen, Terry C. C. Lim Kam Sian, Kimberley C. Clark, Kaylene J. Simpson, Xiuquan Ma, Roger J. Daly
Summary: We found that the expression of pseudokinase NRBP1 is associated with poor prognosis in triple negative breast cancer (TNBC). NRBP1 is required for efficient migration, invasion and proliferation of TNBC cells in culture, as well as growth of TNBC orthotopic xenografts and experimental metastasis. NRBP1 interacted with P-Rex1, Rac1, and Cdc42, and its overexpression enhanced levels of GTP-bound Rac1 and Cdc42 in a P-Rex1-dependent manner, leading to increased cell migration and invasion.
Review
Biochemistry & Molecular Biology
Steven M. Heaton, Paul R. Gorry, Natalie A. Borg
Summary: Antiretroviral therapy (ART) is effective in reducing HIV-1 infection. However, the emergence of treatment-refractory variants remains a challenge. HIV-1 uses cellular helicases, including DDX3X, to facilitate its replication. Exploring these helicases may reveal new therapeutic targets and help prevent viral escape.
TRENDS IN MICROBIOLOGY
(2023)
Article
Physiology
Christina Sul, Caitlin Lewis, Nathan Dee, Nana Burns, Kaori Oshima, Eric Schmidt, Christine Vohwinkel, Eva Nozik
Summary: Acute respiratory distress syndrome (ARDS) is a significant cause of morbidity and mortality in critically ill patients. This study investigates the protective effects of the R213G variant of extracellular superoxide dismutase (EC-SOD) in a methicillin-resistant S. aureus (MRSA) pneumonia model. The results suggest that the R213G EC-SOD variant preserves the integrity of the alveolar-capillary interface, attenuates proinflammatory cytokine levels, reduces neutrophil recruitment and neutrophil extracellular trap (NET) generation, and prevents extrapulmonary dissemination of bacteria.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Caitlin V. Lewis, Hassan Sellak, Mariem A. Sawan, Giji Joseph, Trevor M. Darby, David VanInsberghe, Crystal R. Naudin, David R. Archer, Rheinallt M. Jones, W. Robert Taylor
Summary: This study found that sickle cell disease (SCD) mice have compromised intestinal barrier function, inflammation, and dysbiosis. The small intestine of SCD mice showed increased intestinal permeability and decreased expression of tight junction proteins. In addition, there was an increase in neutrophils, pro-inflammatory gene expression, oxidative stress, and dysbiosis in the small intestine. These observations were not seen in the colon.
Article
Cardiac & Cardiovascular Systems
Julian Hurtado, Hassan Sellak, Giji Joseph, Caitlin V. Lewis, Crystal R. Naudin, Sergio Garcia, James Robert Wodicka, David R. Archer, W. Robert Taylor
Summary: This study demonstrates that beta-thalassemia leads to accelerated atherosclerosis. Hemopexin therapy reduces plaque accumulation in both beta-thalassemia and phenylhydrazine-treated mice.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Rob Hill, Julie Sanchez, Laura Lemel, Mirjana Antonijevic, Yselkla Hosking, Shailesh N. Mistry, Andrew C. Kruegel, Jonathan A. Javitch, J. Robert Lane, Meritxell Canals
Summary: This study evaluated the effects of three novel opioids on respiratory depression and analgesia, and compared these measurements with their in vitro efficacy. The results showed that these opioids can induce respiratory depression and analgesia at certain doses, but there are differences in potency and duration of effect among the novel opioids.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Macsue Jacques, Shanie Landen, Javier Alvarez Romero, Danielle Hiam, Ralf B. Schittenhelm, Iresha Hanchapola, Anup D. Shah, Sarah Voisin, Nir Eynon
Summary: Exercise has significant effects on both the epigenome and proteome in human muscle. A 12-week high-intensity interval training intervention was conducted on 16 healthy moderately trained males, and muscle biopsies were collected at baseline and after 4, 8, and 12 weeks. The study found significant changes in proteins and DNA methylation levels, with some proteins consistently changed across individuals and the discovery of two novel exercise-related proteins. These findings contribute to a better understanding of the impact of exercise on the human body.
Article
Biochemical Research Methods
Haijian Zhang, Joel R. Steele, Hossein Valipour Kahrood, Deanna Deveson Lucas, Anup D. Shah, Ralf B. Schittenhelm
Summary: Phosphoproteomics is a comprehensive method for identifying and quantifying phosphorylated peptides and their associated proteins. Phospho-Analyst is an easy-to-use web application that simplifies the statistical analysis and interpretation of phosphoproteomic data. It also automatically normalizes the results to protein abundance levels, ensuring the consideration of genuine phosphorylation changes.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Food Science & Technology
Sarah A. Marshall, Remy B. Young, Jessica M. Lewis, Emily L. Rutten, Jodee Gould, Christopher K. Barlow, Cristina Giogha, Vanessa R. Marcelino, Neville Fields, Ralf B. Schittenhelm, Elizabeth L. Hartland, Nichollas E. Scott, Samuel C. Forster, Emily L. Gulliver
Summary: Sulforaphane promotes the growth of specific gut microbiota and produces anti-inflammatory metabolites, providing a new mechanism for treating inflammatory states.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Multidisciplinary Sciences
Louise Uoselis, Runa Lindblom, Wai Kit Lam, Catharina J. Kung, Marvin Skulsuppaisarn, Grace Khuu, Thanh N. Nguyen, Danielle L. Rudler, Aleksandra Filipovska, Ralf B. Schittenhelm, Michael Lazarou
Summary: This study developed a functional proteomics framework called MitoPQ to investigate the role of UPRmt in maintaining proteostasis during stress. The UPRmt was found to play critical roles in protecting and repairing proteostasis, especially in oxidative phosphorylation metabolism. Additionally, the interaction between the UPRmt and PINK1/Parkin mitophagy promotes the recovery of oxidative phosphorylation when the UPRmt fails.
Article
Biology
Ghizal Siddiqui, Amanda De Paoli, Christopher A. MacRaild, Anna E. Sexton, Coralie Boulet, Anup D. Shah, Mitchell B. Batty, Ralf B. Schittenhelm, Teresa G. Carvalho, Darren J. Creek
Summary: A comprehensive spectral library for Plasmodium falciparum-infected RBCs has been created, measuring the abundance of peptides from both the parasite and RBC proteins. This library includes proteins from different RBC stages, the RBC compartment of trophozoite-iRBCs, and cytosolic fraction from uninfected RBCs. By using this library, semi-quantitative proteomics datasets have been generated to characterize different asexual parasite stages and compare drug-resistant and drug-sensitive parasites.