Article
Endocrinology & Metabolism
Jinghuan Fang, Michael Chopp, Hongqi Xin, Li Zhang, Fengjie Wang, William Golembieski, Zheng Gang Zhang, Li He, Zhongwu Liu
Summary: Plasminogen deficiency in mice resulted in reduced functional recovery and decreased vessel density in the peri-infarct area post-stroke. In vitro experiments showed that cerebral endothelial cells from plasminogen knockout mice exhibited significantly impaired angiogenesis and migration abilities. Additionally, the expression of thrombospondin-1 and thrombospondin-2 was increased in the plasminogen knockout group after stroke, particularly in the ipsilesional side of the brain.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Review
Cell Biology
James Petrik, Sylvia Lauks, Bianca Garlisi, Jack Lawler
Summary: Many cancers start with a small nest of transformed cells that can remain dormant. Thrombospondin-1 (TSP-1) initially promotes dormancy by suppressing angiogenesis, but over time, factors promoting angiogenesis become dominant and recruit various cells to form a complex tumor microenvironment. TSPs play a role in the proliferation, migration, and invasion of cells in the tumor microenvironment, as well as influencing the immune characteristics and phenotype of tumor-associated macrophages.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Veronica Morandi, Jim Petrik, Jack Lawler
Summary: Thrombospondins (TSPs) are a family of extracellular matrix proteins that regulate cellular behavior and influence biological processes such as angiogenesis and cancer progression. Thrombospondin 1 (TSP-1) interacts with cell surface receptors to have varying effects on cellular behavior depending on the context. Specific membrane microdomains containing receptors like VEGFR2 and Src family kinases are modulated by TSP-1, impacting endothelial cell signal transduction and angiogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yixiao Pan, Lu Deng, Hai Wang, Kang He, Qiang Xia
Summary: Histidine-rich glycoprotein (HRGP) is a glycoprotein abundant in plasma with a multidomain structure, allowing it to interact with various ligands and regulate biological processes. HRGP acts as an extracellular adaptor protein, connecting with different ligands such as heme, Zn2+, thrombospondin.
Article
Chemistry, Analytical
Ping Luo, Kuncheng Zhou, Gang Li, Tianming Tao, Ji Tao, Ray P. S. Han, Yuanbiao Tu
Summary: Surgical resection is the preferred treatment for colorectal cancer, but effective targeting probes for imaging-guided surgical navigation are lacking. In this study, a fluorescent probe ABT-510 labeled with fluorescein isothiocyanate or near-infrared dye MPA was developed and found to have high selectivity and specificity for cells or tissues with high CD36 expression. The probe showed excellent tumor delineation characteristics and antiangiogenic effects, making it a desirable tool for CRC imaging and surgical navigation.
ANALYTICAL CHEMISTRY
(2023)
Article
Gastroenterology & Hepatology
Guido Carpino, Vincenzo Cardinale, Alessandra Di Giamberardino, Diletta Overi, Samantha Donsante, Tania Colasanti, Gaia Amato, Gianluca Mennini, Matteo Franchitto, Fabrizio Conti, Massimo Rossi, Mara Riminucci, Eugenio Gaudio, Domenico Alvaro, Carmine Mancone
Summary: The study identified THBS1, THBS2, and PEDF released in iCCA inhibit vessel growth and promote tumor-associated lymphangiogenesis, offering potential targets for interventions to counteract the dissemination process in iCCA.
JOURNAL OF HEPATOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Ken-Go Hayashi, Ryosuke Sakumoto
Summary: This study aimed to investigate the mRNA expression of pro- and anti-angiogenic factors, protein localization of thrombospondin (TSP), and vascularity in the endometrium of repeat breeder (RB) and normally fertile (non-RB) cows. RB cows had higher mRNA expression levels of TSP ligands and receptors than non-RB cows. RB cows also had higher mRNA expression of FGFR1, ANGPT1, and ANGPT2, and lower mRNA expression of VEGFB than non-RB cows.
ANIMAL REPRODUCTION SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Sukhbir Kaur, Steven M. Bronson, Dipasmita Pal-Nath, Thomas W. Miller, David R. Soto-Pantoja, David D. Roberts
Summary: Thrombospondin-1 has diverse roles in cancer biology, including regulating angiogenesis, immune suppression, and genotoxic stress responses. Challenges remain in harnessing its therapeutic potential in cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Jiayu Wang, Tao Sun, Quchang Ouyang, Yiqun Han, Binghe Xu
Summary: This study investigated the safety and preliminary efficacy of TQB2450, an anti-PD-L1 antibody, combined with anlotinib, a multi-kinase inhibitor, in advanced TNBC. The results showed that the combination therapy of TQB2450 and anlotinib as a chemotherapy-free treatment demonstrated promising efficacy with a manageable safety profile for previously treated advanced TNBC patients.
Article
Pharmacology & Pharmacy
Yahan Ju, Zhimin Tang, Xiaochan Dai, Huiqin Gao, Jing Zhang, Yan Liu, Yanan Yang, Ni Ni, Dandan Zhang, Yuyao Wang, Na Sun, Luqiao Yin, Min Luo, Jianhua Zhang, Ping Gu
Summary: Thrombospondin-1 has dual anti-inflammatory and anti-angiogenic properties, making it a promising agent for protecting against blue light-induced retinal damage and retinal degenerative disorders that are pathologically associated with inflammatory and angiogenic processes.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Cell Biology
Sze-Wan Shan, Chi-Wai Do, Thomas Chuen Lam, Hoi-Lam Li, W. Daniel Stamer, Chi-Ho To
Summary: The selective ROCK inhibitor Y39983 induces actin remodeling, decreases cell motility and transepithelial resistance in human TM cells. Proteomic analysis identified 20 significantly altered proteins after Y39983 treatment, involving pathways such as p53, integrin signaling, and cytoskeletal regulation by Rho GTPase. Downregulation of TSP1 by Y39983 and its antagonists led to reduced cell migration and increased outflow facility.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Medicine, Research & Experimental
Ju Huang, Congcong Wang, Yixuan Hou, Yuanyuan Tian, Yanru Li, Haiying Zhang, Lihong Zhang, Wei Li
Summary: This review discusses the differences between vasculogenic mimicry (VM) and the classical tumor angiogenesis model, as well as the impact of VM on prognosis and survival rates in patients with highly aggressive cancer. The review also highlights the limitations of traditional anti-tumor angiogenesis therapy and the important role of thrombospondin 2 (THBS2) in angiogenesis. Furthermore, the review explores the mechanisms by which THBS2 participates in and regulates tumor VM through the activation of the PI3K/AKT/mTOR signaling pathway.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cell Biology
Xiaojing Cui, Cuiping Zhang, Fang Wang, Xinghui Zhao, Shuxia Wang, Jinpeng Liu, Daheng He, Chi Wang, Feng-Chun Yang, Sheng Tong, Ying Liang
Summary: Hematopoietic stem cells (HSCs) have sex differences in their function and hematopoiesis. Deletion of latexin (Lxn) gene promoted HSC survival and repopulation capacity in female mice, but showed no effect in male mice. Male-specific high expression of microRNA 98-3p (miR98-3p) contributed to the suppression of Thbs1 gene in male HSCs, leading to the abrogation of Lxn's functional effect on HSCs and hematopoiesis. These findings reveal a regulatory mechanism involving sex-chromosome-related microRNA and its differential control of Lxn-Thbs1 signaling in hematopoiesis, contributing to the understanding of sex dimorphism in hematopoiesis.
Article
Oncology
Jack Lawler
Summary: Considerable progress has been made in understanding angiogenesis in normal and tumor tissue. The success of anti-angiogenic treatments has been limited so far, but it is expected to improve with the development of therapies targeting other pathways and a better understanding of resistance.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Chi-Ho Lee, Wai-Kay Seto, David Tak-Wai Lui, Carol Ho-Yi Fong, Helen Yilin Wan, Chloe Yu-Yan Cheung, Wing-Sun Chow, Yu-Cho Woo, Man-Fung Yuen, Aimin Xu, Karen Siu-Ling Lam
Summary: Circulating TSP2 level is associated with both the presence and development of advanced fibrosis in patients with type 2 diabetes and NAFLD, suggesting that it may be a potentially useful prognostic biomarker for liver fibrosis progression.
Article
Chemistry, Multidisciplinary
Stefan Woerner, Bernhard N. Bohnert, Matthias Woern, Mengyun Xiao, Andrea Janessa, Andreas L. Birkenfeld, Kerstin Amann, Christoph Daniel, Ferruh Artunc
Summary: High doses of aprotinin exert nephrotoxic effects in healthy mice by accumulating in the tubular system, inhibiting proximal tubular function, and counterregulatory stimulation of ENaC-mediated sodium transport. Under a low-salt diet, aprotinin impaired sodium preservation, stimulated excessive hyperaldosteronism, and unexpectedly, activated ENaC.
ACTA PHARMACOLOGICA SINICA
(2022)
Review
Biochemistry & Molecular Biology
Maximilian Ackermann, Hans-Joachim Anders, Rostyslav Bilyy, Gary L. Bowlin, Christoph Daniel, Rebecca De Lorenzo, Mikala Egeblad, Timo Henneck, Andres Hidalgo, Markus Hoffmann, Bettina Hohberger, Yogendra Kanthi, Mariana J. Kaplan, Jason S. Knight, Jasmin Knopf, Elzbieta Kolaczkowska, Paul Kubes, Moritz Leppkes, Aparna Mahajan, Angelo A. Manfredi, Christian Maueroeder, Norma Maugeri, Ioannis Mitroulis, Luis E. Munoz, Teluguakula Narasaraju, Elisabeth Naschberger, Indira Neeli, Lai Guan Ng, Marko Z. Radic, Konstantinos Ritis, Patrizia Rovere-Querini, Mirco Schapher, Christine Schauer, Hans-Uwe Simon, Jeeshan Singh, Panagiotis Skendros, Konstantin Stark, Michael Stuerzl, Johan van der Vlag, Peter Vandenabeele, Ljubomir Vitkov, Maren von Koeckritz-Blickwede, Cansu Yanginlar, Shida Yousefi, Alexander Zarbock, Georg Schett, Martin Herrmann
Summary: SARS-CoV-2 infection poses a major threat to multiple organs, with severe cases showing dysregulated immune responses. The imbalance between neutrophil extracellular trap (NET) formation and degradation plays a central role in the pathophysiology of severe COVID-19 cases. Therapeutic strategies targeting dysregulated NET formation or degradation may benefit severe COVID-19 patients.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Cell Biology
Bernhard N. Bohnert, Irene Gonzalez-Menendez, Thomas Doerffel, Jonas C. Schneider, Mengyun Xiao, Andrea Janessa, M. Zaher Kalo, Birgit Fehrenbacher, Martin Schaller, Nicolas Casadei, Kerstin Amann, Christoph Daniel, Andreas L. Birkenfeld, Florian Grahammer, Lahoucine Izem, Edward F. Plow, Leticia Quintanilla-Martinez, Ferruh Artunc
Summary: In susceptibility to doxorubicin-induced nephropathy, the interaction between the C6418T SNP in the Prkdc gene and Plg plays a crucial role, following a hierarchical two-hit process in 129S1/SvImJ mice.
DISEASE MODELS & MECHANISMS
(2021)
Article
Cell Biology
Florian Tesch, Florian Siegerist, Eleonora Hay, Nadine Artelt, Christoph Daniel, Kerstin Amann, Uwe Zimmermann, Panagiotis Kavvadas, Olaf Grisk, Christos Chadjichristos, Karlhans Endlich, Christos Chatziantoniou, Nicole Endlich
Summary: Research has shown that the up-regulation of CLDN5 (a tight junction protein) in injured podocyte foot processes is associated with changes in their structure, potentially serving as a biomarker for predicting early foot process effacement.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Urology & Nephrology
Wiebke Pirschel, Antonio N. Mestekemper, Bianka Wissuwa, Nadine Krieg, Sarah Kroller, Christoph Daniel, Florian Gunzer, Emanuela Tolosano, Michael Bauer, Kerstin Amann, Stefan H. Heinemann, Sina M. Coldewey
Summary: Thrombotic microangiopathy, hemolysis and acute kidney injury are typical characteristics of HUS. This study investigated the impact of haptoglobin and hemopexin on HUS. The results showed that haptoglobin deficiency decreased survival rate, while hemopexin deficiency reduced kidney inflammation and thrombotic microangiopathy. Low dose haptoglobin administration attenuated kidney damage. Signs of kidney hemoglobin degradation were observed in HUS.
KIDNEY INTERNATIONAL
(2022)
Article
Immunology
Janina Niederreiter, Christine Eck, Tajana Ries, Arndt Hartmann, Bruno Maerkl, Maike Buettner-Herold, Kerstin Amann, Christoph Daniel
Summary: Complement deposition is increased in the lungs and kidneys of critically ill COVID-19 patients, suggesting its involvement in exacerbating the inflammatory response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, General & Internal
Maike Buettner-Herold, Nathalie Krieglstein, Teresa Chuva, Kaija Minuth, Frederick Pfister, Christoph Daniel, Monika Klewer, Anke Buttner, Fulvia Ferrazzi, Simone Bertz, Kerstin Amann
Summary: This study investigated the significance of light chain (LC) restriction or crystals (LC-R/C) in proximal tubules in the spectrum of LC-induced nephropathies. LC-R/C was found in a quarter of cases analyzed and was strongly associated with cast-NP, suggesting that a high load of clonal LC may be responsible for this phenomenon.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, General & Internal
Eva Vonbrunn, Miriam Angeloni, Maike Buettner-Herold, Janina Mueller-Deile, Katharina Heller, Erik Bleich, Stefan Soellner, Kerstin Amann, Fulvia Ferrazzi, Christoph Daniel
Summary: This study explored the use of gene expression analysis of zero-time biopsies to predict complications in renal transplantation. The results showed marked differences in gene expression profiles between living and deceased donors, with some differentially expressed genes correlating with cold ischemia time. Additionally, immunoglobulin expression was found to be altered in transplants with rejection and previous acute renal failure. Further investigation is needed to confirm these findings in larger cohorts and to clarify the role of early immunoglobulin upregulation in zero-time biopsies.
FRONTIERS IN MEDICINE
(2022)
Article
Genetics & Heredity
Markus Toelle, Cornelia Henkel, Jaqueline Herrmann, Christoph Daniel, Milen Babic, Mengdi Xia, Anna M. Schulz, Kerstin Amann, Markus van der Giet, Mirjam Schuchardt
Summary: This study evaluated an adenine-based uremic mouse model to study medial vessel calcification and senescence-associated secretory phenotype (SASP) changes, providing a basis for understanding molecular pathogenesis and potential therapeutic interventions.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2022)
Article
Cell Biology
Eva-Marie Bichlmayer, Lina Mahl, Leo Hesse, Eric Pion, Victoria Haller, Andreas Moehwald, Christina Hackl, Jens M. Werner, Hans J. Schlitt, Siegfried Schwarz, Philipp Kainz, Christoph Brochhausen, Christian Groeger, Felix Steger, Oliver Koelbl, Christoph Daniel, Kerstin Amann, Andre Kraus, Bjorn Buchholz, Thiha Aung, Silke Haerteis
Summary: Autosomal dominant polycystic kidney disease (ADPKD) is a genetic disorder that leads to the progressive enlargement of kidney cysts and renal failure. Current models for studying human cyst growth and drug trials are limited. In this study, a chorioallantoic membrane (CAM) model was used to culture renal tissue from ADPKD patients and mouse kidney slices, and successfully evaluated cystic tissue growth. The CAM model may provide a valuable platform for bridging the gap between animal studies and clinical trials of human cyst growth.
Article
Pathology
Nassim Bouteldja, David Laurin Hoelscher, Barbara Mara Klinkhammer, Roman David Buelow, Johannes Lotz, Nick Weiss, Christoph Daniel, Kerstin Amann, Peter Boor
Summary: This research proposes a novel concept of stain augmentation to develop stain-independent CNNs for digital pathology. It outperforms state-of-the-art techniques and enables segmentation on immunohistochemical stainings without additional annotations, achieving performance comparable to the annotated stain. Examples of application in inflammation and fibrosis quantification are presented, indicating the effectiveness of stain augmentation in deep-learning segmentation algorithms.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Oncology
Eva Vonbrunn, Maike Buettner-Herold, Kerstin Amann, Christoph Daniel
Summary: Kidney transplantation is a life-saving strategy for end-stage renal disease patients, but long-term organ survival remains a challenge due to surgical techniques, immunosuppression, and organ shortage. The complement system, part of the innate immune system, plays a crucial role in transplantation-related pathological events. Dysregulation or activation of the complement system can lead to tissue damage and inflammation. This review discusses the role of the complement system in transplantation and potential therapeutic approaches.
Article
Biotechnology & Applied Microbiology
Michaela Alexandra Anna Fuchs, Julia Schrankl, Charlotte Wagner, Christoph Daniel, Armin Kurtz, Katharina Anna-Elisabeth Broeker
Summary: This study characterizes the cellular origin of Penk expression in renal fibrosis models and provides fundamental information about the potential new biomarker Penk.
Article
Multidisciplinary Sciences
Sambhasan Banerjee, Julian Baur, Christoph Daniel, Peter Benedikt Pfeiffer, Manuel Hitzenberger, Lukas Kuhn, Sebastian Wiese, Johan Bijzet, Christian Haupt, Kerstin U. Amann, Martin Zacharias, Bouke P. C. Hazenberg, Gunilla T. Westermark, Matthias Schmidt, Marcus Fandrich
Summary: This study reports the cryo-EM structures of AA amyloid fibrils from two patients with vascular AA amyloidosis, indicating that different disease variants in systemic amyloidosis are associated with different fibril structures.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Salvador Cazorla-Vazquez, Peter Koesters, Simone Bertz, Frederick Pfister, Christoph Daniel, Mark Dedden, Sebastian Zundler, Tilman Jobst-Schwan, Kerstin Amann, Felix B. Engel
Summary: In this study, the expression of Gpr126 in zebrafish, mice, and humans during kidney development and adulthood was characterized using RNAscope technology. The results showed that Gpr126 was enriched in the epithelial lineage during nephrogenesis and persisted in various cell types in the adult kidney. Single-cell RNA sequencing analysis revealed that Gpr126 expression was detected in a distinct ionocyte sub-population in zebrafish, which is involved in acid secretion, pH buffering, and calcium regulation. The expression patterns of Gpr126 and related genes in mouse kidneys were highly similar. These findings provide insights into the spatio-temporal expression and potential physiological role of Gpr126 in the kidney.