Controlled Fab-arm exchange for the generation of stable bispecific IgG1

Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface

(2014) Steven M Lewis et al.   NATURE BIOTECHNOLOGY

Knobs-into-holes antibody production in mammalian cell lines reveals that asymmetric afucosylation is sufficient for full antibody-dependent cellular cytotoxicity

(2013) Whitney Shatz et al.   mAbs

Production of stable bispecific IgG1 by controlled Fab-arm exchange

(2013) Michael J Gramer et al.   mAbs

Protein engineering and the use of molecular modeling and simulation: The case of heterodimeric Fc engineering

(2013) Thomas Spreter Von Kreudenstein et al.   METHODS

A simple, robust and highly efficient transient expression system for producing antibodies

(2013) Tom Vink et al.   METHODS

Boosting ADCC and CDC activity by Fc engineering and evaluation of antibody effector functions

(2013) Christian Kellner et al.   METHODS

A Heterodimeric Fc-Based Bispecific Antibody Simultaneously Targeting VEGFR-2 and Met Exhibits Potent Antitumor Activity

(2013) H.-J. Choi et al.   MOLECULAR CANCER THERAPEUTICS

Bispecific antibodies with natural architecture produced by co-culture of bacteria expressing two distinct half-antibodies

(2013) Christoph Spiess et al.   NATURE BIOTECHNOLOGY

Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange

(2013) A. F. Labrijn et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generating Bispecific Human IgG1 and IgG2 Antibodies from Any Antibody Pair

(2012) Pavel Strop et al.   JOURNAL OF MOLECULAR BIOLOGY

A novel bispecific antibody format enables simultaneous bivalent and monovalent co-engagement of distinct target antigens

(2012) Gregory L. Moore et al.   mAbs

Dual targeting strategies with bispecific antibodies

(2012) Roland Kontermann   mAbs

Species-Specific Determinants in the IgG CH3 Domain Enable Fab-Arm Exchange by Affecting the Noncovalent CH3-CH3 Interaction Strength

(2011) A. F. Labrijn et al.   JOURNAL OF IMMUNOLOGY

Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies

(2011) W. Schaefer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhancing Antibody Fc Heterodimer Formation through Electrostatic Steering Effects

(2010) Kannan Gunasekaran et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular construction and optimization of anti-human IL-1α/β dual variable domain immunoglobulin (DVD-IgTM) molecules

(2010) Chengbin Wu et al.   mAbs

SEEDbodies: fusion proteins based on strand-exchange engineered domain (SEED) CH3 heterodimers in an Fc analogue platform for asymmetric binders or immunofusions and bispecific antibodies†

(2010) Jonathan H. Davis et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Formulation Development of Antibodies Using Robotic System and High-Throughput Laboratory (HTL)

(2009) Hui Zhao et al.   JOURNAL OF PHARMACEUTICAL SCIENCES