Article
Chemistry, Applied
Sze Ying Lee, Bing-Lan Liu, Jun-Yi Wu, Yu-Kaung Chang
Summary: In this study, a weak ion-exchange membrane (P-COOH) was synthesized through alkaline hydrolysis and successfully used for the purification of lysozyme from chicken egg white with a high recovery yield of 98%. The scalability of the purification technique was demonstrated by achieving consistent results with different feedstock loading volumes and operating conditions.
Article
Biochemical Research Methods
Myriam Belen Gonzalez Viacava, Carlos Leonidas Leiva, Maria Eugenia Tavarone, Jose Christian Dokmetjian, Osvaldo Cascone, Pablo Chacana, Matias Fingermann
Summary: This article describes a three-step process for Fab' production from crude egg yolk water-soluble fractions using scalable and simple low-cost technologies. The process can achieve high purity of Fab' with a overall process yield comparable to medicinal products produced from mammalian antibodies.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Emanuel Peplau, Francesco De Rose, Andreas Eichinger, Sybille Reder, Markus Mittelhaeuser, Giorgia Scafetta, Markus Schwaiger, Wolfgang A. Weber, Armando Bartolazzi, Calogero D'Alessandria, Arne Skerra
Summary: The study developed a PASylated humanized alpha hGal3 Fab for diagnostic imaging of thyroid cancer, achieving high sensitivity and specificity imaging of tumors through effective immunohistochemical staining and radiotracer labeling.
SCIENTIFIC REPORTS
(2021)
Article
Green & Sustainable Science & Technology
Beixin Xia, Tong Tian, Yan Gao, Mingyue Zhang, Yunfang Peng
Summary: This paper proposes an efficient multi-objective dynamic dispatching method for Interbay Automated Material Handling Systems (AMHS) in Semiconductor Wafer Fabrication Systems (SWFS). The method adjusts vehicle-load assignments according to the real-time situation of the system, resulting in improved performance compared to previous methods.
Article
Biochemical Research Methods
Lucas K. Kimerer, Ben Niu, Timothy M. Pabst, Weiguo Zhai, Alan K. Hunter, Giorgio Carta
Summary: The study on elution and adsorptive behavior of a bivalent bispecific antibody and associated fragments on two cation exchange chromatography media revealed that configurational flexibility affects multi-peak elution behavior, while mass transfer limitations lead to broader peaks that obscure the multi-peak elution behavior.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Pharmacology & Pharmacy
Amarande Murisier, Valentina D'Atri, Sebastian Pirner, Vincent Larraillet, Szabolcs Fekete, Matthew Lauber, Davy Guillarme
Summary: This study explores the benefits and limitations of using ultra-short RPLC columns for the characterization of complex bispecific antibodies under prolonged thermal stress. The results show that alternative organic modifiers can be used without excessive pressure in RPLC mode, but they do not improve selectivity compared to acetonitrile. Spectroscopic detector allows for fast and high-quality separations, while mass spectrometry detection leads to performance loss due to dispersion effects. Therefore, improving the interface between LC and MS is necessary to mitigate band broadening.
Review
Pharmacology & Pharmacy
Maria Camilla Operti, Alexander Bernhardt, Silko Grimm, Andrea Engel, Carl Gustav Figdor, Oya Tagit
Summary: Nanomedicines based on PLGA carriers show great potential for biomedical research, but are not yet available on the global market. Challenges include scaling up production and successful translation into clinical practice, requiring advancements in manufacturing techniques and quality control methods.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Food Science & Technology
Gabor Endre, Babett Edit Nagy, Daniel Hercegfalvi, Csenge Kasuba, Csaba Vagvolgyi, Andras Szekeres
Summary: Aflatoxins (AFs) are secondary metabolites that cause diseases in animals and humans. Regulations set concentration limits for AFs in EU food and feed products, requiring pure forms for reference standards. Improvements were made to a liquid-liquid chromatographic method to enhance purification and increase the yield of pure AFs. Through a scale-up process, approximately 2.2 g of AFs could be purified using 8.2 L of solvent in a 250 mL rotor, while 7.8 g of AFs could be prepared using 31 L of solvent in a 1000 mL rotor.
Article
Biochemical Research Methods
Lucas K. Kimerer, Timothy M. Pabst, Alan K. Hunter, Giorgio Carta
Summary: The adsorption kinetics of mAb is controlled by pore diffusion, while BiSAb's structure complexity leads to multiple binding forms influenced by buffer conditions and temperature. Temperature affects both BiSAb pore diffusivity and the interconversion kinetics between binding forms.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biotechnology & Applied Microbiology
David Humbird
Summary: The analysis reveals that due to various limitations in animal cell production, the current costs of mass producing cultured meat products are high, making it difficult to replace conventional meat. Improving metabolic efficiency and developing low-cost plant hydrolysates as media do help address the issue, but there are still challenges ahead.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Pharmacology & Pharmacy
Steven A. Ross, Andrew P. Hurt, Milan Antonijevic, Nicolaos Bouropoulos, Adam Ward, Pat Basford, Mark McAllister, Dennis Douroumis
Summary: The aim of the study was to manufacture and scale up the theophylline-nicotinamide pharmaceutical cocrystals using hot-melt extrusion. Critical processing parameters were identified, and high purity cocrystals were formed. The quality of the cocrystals remained unchanged under accelerated stability conditions.
Article
Pharmacology & Pharmacy
Marcel Franke, Thomas Riedel, Robin Meier, Carsten Schmidt, Peter Kleinebudde
Summary: The aim of this study was to compare different scale-up strategies in twin-screw wet granulation and investigate the impact of the selected strategy on granule and tablet properties for a defined formulation. Three different scale-up strategies were introduced based on differences in process parameters and their resulting effects on various aspects. Despite major differences in process parameters, granule and tablet properties were similar after milling on both scales and with all applied strategies.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Engineering, Biomedical
Debbie L. L. Ho, Stacey Lee, Jianyi Du, Jonathan D. D. Weiss, Tony Tam, Soham Sinha, Danielle Klinger, Sean Devine, Art Hamfeldt, Hope T. T. Leng, Jessica E. E. Herrmann, Mengdi He, Lee G. G. Fradkin, Tze Kai Tan, David Standish, Peter Tomasello, Donald Traul, Noushin Dianat, Rukmini Ladi, Quentin Vicard, Kishore Katikireddy, Mark A. A. Skylar-Scott
Summary: By combining sustainable culture of billions of human cells and bioprinting of cellular bioinks, this study demonstrates an approach to tissue engineering at organ-scale. The research optimized the suspension culture and successfully scaled it up, while maintaining pluripotency, and then used 3D bioprinting to differentiate the pluripotent aggregates into different cell lines.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Jungsoon Lee, Zhuyun Liu, Wen-Hsiang Chen, Junfei Wei, Rakhi Kundu, Rakesh Adhikari, Joanne Altieri Rivera, Portia M. Gillespie, Ulrich Strych, Bin Zhan, Peter J. Hotez, Maria Elena Bottazzi
Summary: The study presented the development and scale-up optimization of production and purification process for RBD219-N1C1 protein antigen against SARS-CoV-2, with the selected process yielding high purity and recovery rate of the product. Different chromatographic purification methods were compared, and the best process was chosen based on analytical studies. An optimized production process was proposed, which can generate high-quality RBD219-N1C1 protein antigen at a low cost and scalable level.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Medicine, Research & Experimental
Desislava Yanakieva, Lukas Pekar, Andreas Evers, Markus Fleischer, Stephan Keller, Dirk Mueller-Pompalla, Lars Toleikis, Harald Kolmar, Stefan Zielonka, Simon Krah
Summary: The study expands the cFAE toolbox by incorporating VHH-modules, enabling the generation of tri- and tetraspecific molecules with flexible valencies. The manufacturability and physicochemical characteristics of these molecules were assessed, demonstrating the utility of this approach and its potential in therapeutic applications.