Screening and large-scale expression of membrane proteins in mammalian cells for structural studies

X-Ray Structure of Acid-Sensing Ion Channel 1–Snake Toxin Complex Reveals Open State of a Na+-Selective Channel

(2014) Isabelle Baconguis et al.   CELL

NMDA receptor structures reveal subunit arrangement and pore architecture

(2014) Chia-Hsueh Lee et al.   NATURE

The structural basis of R-spondin recognition by LGR5 and RNF43

(2013) P.-H. Chen et al.   GENES & DEVELOPMENT

Structure of the TRPV1 ion channel determined by electron cryo-microscopy

(2013) Maofu Liao et al.   NATURE

X-ray structure of dopamine transporter elucidates antidepressant mechanism

(2013) Aravind Penmatsa et al.   NATURE

The mechanism of shared but distinct CSF-1R signaling by the non-homologous cytokines IL-34 and CSF-1

(2012) Heli Liu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Identification of a novel enhancer that binds Sp1 and contributes to induction of cold-inducible RNA-binding protein (cirp) expression in mammalian cells

(2012) Yasuhiko Sumitomo et al.   BMC BIOTECHNOLOGY

Overexpression of membrane proteins in mammalian cells for structural studies

(2012) Juni Andréll et al.   MOLECULAR MEMBRANE BIOLOGY

Structural plasticity and dynamic selectivity of acid-sensing ion channel–spider toxin complexes

(2012) Isabelle Baconguis et al.   NATURE

Molecular mechanism of ATP binding and ion channel activation in P2X receptors

(2012) Motoyuki Hattori et al.   NATURE

Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells

(2012) Sarika Chaudhary et al.   Nature Protocols

A Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Protein Precrystallization Screening

(2012) Motoyuki Hattori et al.   STRUCTURE

Principles of activation and permeation in an anion-selective Cys-loop receptor

(2011) Ryan E. Hibbs et al.   NATURE

Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II

(2011) X. Deupi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Snapshots of Full-Length Jak1, a Transmembrane gp130/IL-6/IL-6Rα Cytokine Receptor Complex, and the Receptor-Jak1 Holocomplex

(2011) Patrick J. Lupardus et al.   STRUCTURE

Evaluation of the Virus Counter® for rapid baculovirus quantitation

(2010) Matthew M. Ferris et al.   JOURNAL OF VIROLOGICAL METHODS

Maltose–neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins

(2010) Pil Seok Chae et al.   NATURE METHODS

A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line

(2009) Ralph F. Hopkins et al.   BIOTECHNIQUES

Efficient gene delivery into mammalian cells by recombinant baculovirus containing a hybrid cytomegalovirus promoter/Semliki Forest virus replicon

(2009) Yongfei Pan et al.   JOURNAL OF GENE MEDICINE

Pore architecture and ion sites in acid-sensing ion channels and P2X receptors

(2009) Eric B. Gonzales et al.   NATURE

X-ray structure, symmetry and mechanism of an AMPA-subtype glutamate receptor

(2009) Alexander I. Sobolevsky et al.   NATURE

Structural basis of receptor sharing by interleukin 17 cytokines

(2009) Lauren K Ely et al.   NATURE IMMUNOLOGY

An efficient platform for screening expression and crystallization of glycoproteins produced in human cells

(2009) Jeffrey E Lee et al.   Nature Protocols

The titerless infected-cells preservation and scale-up (TIPS) method for large-scale production of NO-sensitive human soluble guanylate cyclase (sGC) from insect cells infected with recombinant baculovirus

(2009) David J. Wasilko et al.   PROTEIN EXPRESSION AND PURIFICATION

An improved in vitro and in vivo Sindbis virus expression system through host and virus engineering

(2009) Toey Nivitchanyong et al.   VIRUS RESEARCH

Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures

(2008) Gaurav Backliwal et al.   BIOTECHNOLOGY AND BIOENGINEERING

Mild Hypothermia Improves Transient Gene Expression Yields Several Fold in Chinese Hamster Ovary Cells

(2008) S. Wulhfard et al.   BIOTECHNOLOGY PROGRESS

Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor

(2008) F. Magnani et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conformational thermostabilization of the  1-adrenergic receptor in a detergent-resistant form

(2008) M. J. Serrano-Vega et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies

(2008) Abhiram Dukkipati et al.   PROTEIN EXPRESSION AND PURIFICATION

Cloning and pharmacological characterization of the guinea pig P2X7receptor orthologue

(2007) E Fonfria et al.   BRITISH JOURNAL OF PHARMACOLOGY