Article
Education, Scientific Disciplines
Swarnendu Basak, Hae-Ji Kang, Ki-Back Chu, Judy Oh, Fu-Shi Quan
Summary: A simple, rapid, and accurate plaque assay method using immunostaining was established for visualizing plaques in Sf9 cells infected with recombinant baculoviruses. Variations in cell seeding densities led to differences in final plaque quantification, highlighting the importance of standardizing cell concentrations in baculovirus-related studies.
Article
Immunology
Keon-Woong Yoon, Ki-Back Chu, Hae-Ji Kang, Min-Ju Kim, Gi-Deok Eom, Jie Mao, Su-Hwa Lee, Md Atique Ahmed, Fu-Shi Quan
Summary: This study found that oral immunization with Toxoplasma gondii vaccines induced a similarly effective immune response as intranasal or intramuscular immunizations, providing a protective effect against lethal infections in mice.
Article
Microbiology
Leonardo Assis da Silva, Monique da Rocha Queiroz Lima, Brenda Rabello de Camargo, Dyeferson Kened da Silva Coelho Guimaraes, Anabele Azevedo Lima Barbastefano, Raquel Curtinhas de Lima, Paulo Vieira Damasco, Rivaldo Venancio da Cunha, Luiz Jose de Souza, Elzinandes Leal de Azeredo, Luzia Maria De-Oliveira-Pinto, Tatsuya Nagata, Daniel M. P. Ardisson-Araujo, Flavia Barreto dos Santos, Bergmann Morais Ribeiro
Summary: CHIKV is a globally distributed arbovirus, and its laboratory diagnosis is mainly performed by molecular and serological assays. This research developed an ELISA test based on a multi-epitope recombinant protein for specific detection of anti-CHIKV antibodies, showing high sensitivity and specificity with no cross-reactivity.
Article
Immunology
Manon M. J. Cox
Summary: The insect cell expression system, proposed as the preferred biosecurity strategy for vaccine production, has limitations that need to be addressed for effective use in future pandemics.
Article
Multidisciplinary Sciences
Alessandra Pratt, Nile Eckermann, Shankar Rengasamy Venugopalan, Lina Moreno Uribe, Lauren Barlow, Matthew Nonnenmann
Summary: The aim of this study was to quantify the aerosol concentrations of bacteriophage MS2 near the oral cavity and behind the dental practitioner's face shield during a simulated orthodontic debanding procedure. The results showed that debanding generates live virus aerosol and face shields do not provide significant protection from virus aerosol, but they do offer some protection against splatter during the procedure.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Muhammed Muhsin Varikkodan, Chun-Chung Chen, Tzong-Yuan Wu
Summary: Chikungunya virus (CHIKV) is a global threat due to its high contagion rate and lack of effective remedies. Two BEVS-based approaches were developed to screen anti-CHIKV drugs, with one involving cell fusion in insect cells and the other using a replicon system in human cells to identify potential inhibitory compounds. The results suggest that BEVS could be a valuable tool for drug screening against CHIKV.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Chemical
Carina Bannach, Daniel Ruiz Buck, Genna Bobby, Leo P. Graves, Sainan Li, Adam C. Chambers, Elizabeth Gan, Raquel Arinto-Garcia, Robert D. Possee, Linda A. King
Summary: Autographa californica nucleopolyhedrovirus is an efficient expression vector for recombinant proteins in insect cells. The study found that using the AUA codon may slightly improve protein yield, while the AUU codon may lead to leaky translation.
Article
Biochemical Research Methods
Nikolaus Virgolini, Ryan Hagan, Ricardo Correia, Marco Silvano, Sofia Fernandes, Paula M. Alves, Colin Clarke, Antonio Roldao, Ines A. Isidro
Summary: The insect cell-baculovirus expression vector system (IC-BEVS) is an alternative platform for efficient production of recombinant AAV for gene therapy. Understanding the biological mechanisms of IC-BEVS is crucial for optimizing the system and improving product yield and quality.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Immunology
Kiju Kim, Tae-Wook Hahn
Summary: The study evaluated the protective immune responses of an experimental PCV2d-based subunit vaccine in pigs and found that the vaccine significantly increased PCV2b- or PCV2d-specific IgG and neutralizing antibody levels without interference by maternal antibodies. Additionally, vaccination with the PCV2d-based subunit vaccine led to a significant increase in serum IL-4 levels and a reduction in PCV2d viremia levels compared to the control group.
Article
Agronomy
Sana Bashir, Sumaira Farrakh, Tayyaba Yasmin, Aish Muhammad, Tasmia Bashir, Hakim Manghwar, Freddy Mora-Poblete, Shehzad Iqbal, Alaa Baazeem, Muhammad Zeeshan Hyder
Summary: Banana bunchy top disease is the most devastating viral disease of bananas. The study found that there were variations in the copy number and transcript levels of the virus in different tissues, which can assist in sensitive disease diagnosis and management.
Article
Agriculture, Multidisciplinary
Wang Peng-fei, Wang Ming, Shi Zhi-bin, Sun Zhen-zhao, Wei Li-li, Liu Zai-si, Wang Shi-da, He Xi-jun, Wang Jing-fei
Summary: This study developed an indirect ELISA assay using a recombinant pB602L protein as the antigen, which showed no cross-reaction with the anti-sera against common pig pathogens in China. The coincidence of this assay with a commercial ELISA kit was 95%. Additionally, the assay was used to detect antibody responses in vaccinated pigs against a specific ASFV strain.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Immunology
Mark R. Bruder, Marc G. Aucoin
Summary: The use of CRISPR-Cas9 technology to introduce indel mutations in the baculovirus expression vector system (BEVS) effectively blocked the release of virus particles and improved the production of virus-like particles (VLPs).
Article
Microbiology
Youpeng Fan, Junhong Wei, Wei Wang, Chunfeng Li, Guoqing Pan, Timothy Keiffer, Jialing Bao, Zeyang Zhou
Summary: The use of a baculovirus-silkworm expression system allows for efficient production of the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. By constructing a recombinant baculovirus and infecting silkworms, a high yield of the target foreign protein can be obtained. However, the viral infection rate of the recombinant baculovirus needs to be improved.
Article
Microbiology
Patrick T. Keiser, Manu Anantpadma, Hilary Staples, Ricardo Carrion, Robert A. Davey
Summary: Efforts to develop effective therapies against filoviruses involve quantifying viable virus amounts using plaque, TCID50, and focus assays. A computer-based automated image-processing method showed focus-forming unit (FFU) assays to be more accurate and faster compared to plaque and TCID50 assays, making it more efficient for high-throughput screening of treatment samples.
Article
Virology
Alexander Malogolovkin, Andrew Davies, Sherif Abouelhadid, Adeline Kerviel, Polly Roy, Andrew K. Falconar
Summary: Researchers constructed and characterized Zika virus-like particles (Zika VLPs) to study the interaction between ZIKV and antibodies. These VLPs showed typical virus morphology and the importance of glycosylation of the E glycoprotein was demonstrated. A new construct showed higher efficacy and protein concentration, making it a potential candidate for vaccine trials.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)
