Article
Agronomy
Chengjie Tu, Pei Xu, Runhua Han, Jing Luo, Letian Xu
Summary: This study assessed the stability of seven commonly used reference genes in Plagiodera versicolora and analyzed their expression under different biotic and abiotic conditions. The results showed that RPS18 and EF1A were the most reliable reference genes in different developmental stages and treatments.
Article
Entomology
Xudong Zhao, Yishu Geng, Tianyi Hu, Yongang Zhao, Suling Yang, Dejun Hao
Summary: The study evaluated the stability of eight reference genes in H. cunea under different conditions and verified the expression of the target gene. The results suggest that these reference genes can be used for gene expression studies in H. cunea and provide a reference for selecting reference genes in other Lepidoptera species.
Article
Physiology
Mengyi Li, Xinan Li, Chao Wang, Qiuchi Li, Saige Zhu, Yunhui Zhang, Xiangrui Li, Fengshan Yang, Xun Zhu
Summary: This study identified suitable qRT-PCR reference gene combinations for R. padi under diverse experimental conditions, improving the accuracy of qRT-PCR analyses and facilitating the standardization of qRT-PCR programs.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yajing Wu, Jiao Zhou, Yaning Liu, Yutong Gu, Hongxia Zhang, Faheem Ahmad, Guochang Wang, Lili Ren
Summary: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a powerful technique for studying gene expression, but it is important to use stable reference genes under different experimental conditions. In this study, we analyzed the stability of eight candidate reference genes in the pine wood nematode (PWN) across different temperature and developmental stages. We confirmed the stability of two genes and found that EF1 gamma was the most stable gene under different conditions.
APPLIED SCIENCES-BASEL
(2022)
Article
Engineering, Biomedical
Qiang Song, Lu Dou, Wenjin Zhang, Yang Peng, Man Huang, Mengyuan Wang
Summary: Novel and reliable RG combinations for breast cancer research were identified using a public RNA-seq dataset, providing a solid foundation for accurate normalization of qRT-PCR results across different breast cancer tissues and cells.
BIOMEDICAL ENGINEERING ONLINE
(2021)
Article
Microbiology
Ning Zhao, Junnan Xu, Lingxia Jiao, Mengzhen Qiu, Jie Zhang, Xinyuan Wei, Mingtao Fan
Summary: This study identified new candidate reference genes and determined the optimal multiple reference gene combinations under different acidic conditions.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Plant Sciences
Umair Ahmed, Qi Xie, Xueping Shi, Bo Zheng
Summary: This article reviews the importance of developing appropriate reference genes (RGs) for accurately quantifying gene expression levels, and discusses the strategies and processes for developing RGs using different techniques and methods. The most commonly used RGs in horticultural plants and their development strategies are also discussed in detail. The information provided in this article will help in designing effective strategies for developing RGs in horticultural plants.
CRITICAL REVIEWS IN PLANT SCIENCES
(2022)
Article
Multidisciplinary Sciences
Long Ma, Ting Jiang, Xiangya Liu, Haijun Xiao, Yingchuan Peng, Wanna Zhang
Summary: This study evaluated the expression stabilities of eight candidate reference genes under different conditions and recommended the optimal suites of reference genes for tissue expression analysis, developmental stages, thermal stress, and pesticide exposure. The findings of this work would facilitate molecular studies of the brassica leaf beetle Phaedon brassicae.
Article
Biochemistry & Molecular Biology
Xue Bai, Tao Chen, Yuan Wu, Mingyong Tang, Zeng-Fu Xu
Summary: This study identified a series of reference genes in tiger nut that can serve as internal controls for gene expression studies, providing convenience for qRT-PCR analysis. Among the various tissues, UCE2, UBL5, and Rubisco showed more stable expression patterns, with TUB4 and UCE2 identified as optimal reference genes for tuber development stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Yao Dai, Yumeng Cao, Jens Kohler, Aiping Lu, Shaohua Xu, Haiyun Wang
Summary: This study identified OAZ1 and hsa-miR-6835-3p as the most reliable individual reference genes for mRNA and miRNA quantification in cancer exosomes. A combination of reference genes - OAZ1/SERF2/MPP1 for mRNA and hsa-miR-6835-3p/hsa-miR-4468-3p for miRNA analyses is recommended for superior accuracy.
Article
Plant Sciences
Abhishek Singh Chauhan, Madhu Tiwari, Yuvraj Indoliya, Shashank Kumar Mishra, Umesh Chandra Lavania, Puneet Singh Chauhan, Debasis Chakrabarty, Rudra Deo Tripathi
Summary: This study used transcriptome data of vetiver roots to identify reference genes and validated them under various abiotic stresses. The findings of this study are important for identifying suitable reference genes in transcriptomic and molecular data analysis concerning various phenotypes related to abiotic stress and developmental aspects.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2023)
Article
Multidisciplinary Sciences
Conglong Lian, Bao Zhang, Jingfan Yang, JinXu Lan, Hao Yang, Kaihua Guo, Jingjing Li, Suiqing Chen
Summary: This study evaluated the expression stability of 12 candidate reference genes in different tissues and under different abiotic stresses in Isodon rubescens. The results identified the most stable reference genes for different tissues and treatments, and highlighted the importance of using appropriate reference genes in gene expression studies. This study provides valuable information for gene functional research in I. rubescens.
SCIENTIFIC REPORTS
(2022)
Article
Horticulture
Yuanjie Zhou, Hui Xia, Xinling Liu, Zhiyi Lin, Yuqi Guo, Honghong Deng, Jin Wang, Lijin Lin, Qunxian Deng, Xiulan Lv, Kunfu Xu, Dong Liang
Summary: Reference genes were analyzed using qRT-PCR in kiwifruit under different conditions, and the optimal reference genes were determined. The study assessed gene expression stability and determined the minimum number of reference genes required for normalization using three common software. The results showed that different combinations of reference genes were needed for different genotypes, tissues, developmental stages, and hormone treatments in kiwifruit, providing a new and reliable option for analyzing gene expression patterns.
Article
Biochemistry & Molecular Biology
Xiu-Mei Dong, Wei Zhang, Shi-Bao Zhang
Summary: This study analyzed the expression profiles of anthocyanin and carotenoids biosynthesis genes in Cymbidium lowianum using qRT-PCR. Reference gene selection and validation were conducted before normalization of gene expression. The study provides reference genes in Cymbidium lowianum and valuable information for understanding the molecular mechanisms of flower color formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Nirmal Kumar Sampathkumar, Venkat Krishnan SundaramID, Prakroothi S. Danthi, Rasha Barakat, Shiden Solomon, Mrityunjoy Mondal, Ivo Carre, Tatiana El Jalkh, Aida Padilla-Ferrer, Julien Grenier, Charbel Massaad, Jacqueline C. Mitchell
Summary: The choice of reference genes heavily influences the assessment of differential gene expression by qPCR. Recent studies suggest using RNA-Seq to identify stable genes and statistical approaches to determine the best reference genes. However, this study demonstrates that the statistical approach to determine the best reference genes from commonly used candidates is more important than preselecting stable genes from RNA-Seq data.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Horticulture
Peng Jia, Rui Yan, Yuan Wang, Feng-huan Gao, Yang Liu, Qing-long Dong, Hao-an Luan, Xue-mei Zhang, Han Li, Su-ping Guo, Guo-hui Qi
Summary: The KNOX gene plays crucial roles in plant development, and this study identified 19 KNOX gene members in kiwifruit. One of the genes, AcKNOX11, is selectively expressed in flower buds and shoots, and its expression can be induced by ABA and melatonin. Functional analysis revealed that AcKNOX11 delays flowering, reduces plant height, alters leaf shape, and inhibits seed germination through ABA-melatonin interaction.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Dan Hua, Wen-Shu Chen, Rong-Yu Rao, Xu-Feng Chen, Huan-Huan Chen, Ning-Wei Lai, Lin-Tong Yang, Xin Ye, Li -Song Chen
Summary: This study revealed that nitrogen deficiency can lead to increased production of reactive oxygen species (ROS) and methylglyoxal (MG), but also enhances the ability of leaves and roots to detoxify these molecules. The effects of nitrogen deficiency on ROS and MG generation and their detoxification systems were found to be different in leaves and roots, with roots being more affected.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Shenghan Yang, Chonlong Chio, Wensheng Qin, Yanxi Pei, Guangpeng Pei, Yuen Zhu, Hua Li
Summary: This study evaluates the immobilization and deactivation of pathogen-produced enzymes and toxic metabolites by biochar in tomato Fusarium wilt and finds that biochar can effectively prevent disease occurrence.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Deborah Pagliaccia, Michelle Ortiz, Michael Rodriguez, Sophia Abbott, Agustina De Francesco, Madison Amador, Valeria Lavagi, Benjamin Maki, Francesca Hopkins, Jonathan Kaplan, Samantha Ying, Georgios Vidalakis
Summary: This study investigates the effectiveness of organic soil amendments (bokashi, biochar, and their combination) in promoting soil health, nutrient availability, and plant growth. The results show that these amendments can significantly alter soil parameters and have positive effects on soil and plant health.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yu-Jeong Kwon, Min Jeong Hong, Minkyu Park, Dawon Jeon, Gah-Hyun Lim, Sungyul Chang, Dong-Hwan Kim, Jin-Baek Kim, Jundae Lee, Yeong Deuk Jo
Summary: Flowing LOCUS T (FT)-like genes play crucial roles in flowering induction in plants. Pepper FT-like genes are mostly expressed in leaves and interact with FLOWERING LOCUS D, promoting or delaying flowering. The unique evolutionary process of pepper FT-like genes provides insights for improving productivity.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Ziwen Su, Juan Yan, Binbin Zhang, Meng Sun, Zhixiang Cai, Zhijun Shen, Ruijuan Ma, Mingliang Yu
Summary: Bagging is a crucial cultivation measure that affects the quality of peach fruit by regulating exposure to light. This study investigated the effects of bagging on the content of phenolic compounds in peach fruit and identified the most sensitive compounds to bagging treatment. The study also explored the regulatory role of bagging on phenolic synthetic-related genes. The results showed that bagging affected the accumulation of different phenolics in different peach cultivars and light intensity played a significant role in the accumulation of anthocyanins and other phenolic substances in peach fruit. Additionally, the expression of structural genes and transcription factors related to phenolic synthesis was regulated by bagging and light conditions. These findings provide a foundation for understanding the regulation mechanism of light on the synthesis of phenolic compounds in peach fruit.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yanfang Zhang, Xinzhi Cui, Zhilei Du, Xiulian Li, Bangdi Liu, Meng Liu, Xiangquan Zeng, Fengjun Guo, Xinguang Fan, Shuyang Sun
Summary: In this study, melatonin treatment effectively improved the aroma quality of apricots during low temperature storage by maintaining firmness and soluble solids content, inhibiting ethylene production, and regulating enzyme activities related to aroma synthesis.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Sang-Mo Kang, Shifa Shaffique, Md. Injamum-Ul-Hoque, Sarah Owdah Alomrani, Yong-Sung Park, In-Jung Lee
Summary: Salinity is a global environmental problem that affects the physiology and morphology of plants. Melatonin has been found to improve photosynthesis, antioxidant activities, and seedling characteristics in milk thistle plants exposed to salinity stress, thereby enhancing their salt tolerance.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Nadia Borgini, Haifa Benmoussa, Mohamed Ghrab, Mehdi Ben Mimoun
Summary: This study investigates the agroclimatic requirements of Prunus species cultivars growing in warm areas using Partial Least Squares regression. The findings reveal that the chilling and heat requirements of the cultivars appear discontinuous, with overlaps or transition periods between the two phases. The warm mean temperatures occurring during the chilling period are the main determinant of the flowering of the studied cultivars.
SCIENTIA HORTICULTURAE
(2024)
Review
Horticulture
Kayise Hypercia Maseko, Thierry Regnier, Belinda Meiring, Obiro Cuthbert Wokadala, Tonna Ashim Anyasi
Summary: Bananas are a significant tropical fruit with diverse cultivars, providing essential minerals, vitamins, and phytochemicals. The Cavendish group is preferred, but identifying suitable cultivars and maturity stages is crucial for various applications.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Godfrey Ouma, Joshua Wanyama, Isa Kabenge, Joseph Jjagwe, Mukulu Diana, John Muyonga
Summary: Irrigation plays a crucial role in increasing eggplant yields amidst climate change, but the deficit irrigation level for optimal growth remains uncertain. This study examined the effect of deficit drip irrigation on eggplant performance and found that irrigating at 75% field capacity maximizes yield and water saving in sandy clay loam soil.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Andrea Marcone, Giorgio Impollonia, Michele Croci, Henri Blandinieres, Stefano Amaducci
Summary: This study used Sentinel-2 satellite images to estimate biophysical and biochemical parameters of spinach crops and found that canopy-level parameters were estimated more accurately. The estimation of the canopy-scale parameter AGB using MCARI showed the highest accuracy, while the estimation of the leaf-scale parameter LCC using NDWI showed the lowest accuracy. At the field scale, the validation results for AGB estimation using SR were the best.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Chengsheng Gong, Guangjun Guo, Baogui Pan, Changzhou Gao, Xianwei Zhu, Shubin Wang, Jinbing Liu, Weiping Diao
Summary: This study investigated the role of key cytokines and metabolic factors in the formation of plant-type traits in pepper. Through integrated analysis of RNA-seq and metabolite determination, the study identified the low content of gibberellin and high content of auxin as important factors causing plant dwarfing, and jasmonic acid as a metabolic factor affecting branch traits. A total of 131 candidate genes involved in metabolite synthesis and the formation of plant-type traits were identified.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Yue Ma, Jia-Chen Hu, Yang Yu, Xin Cheng, Yan-Li Du, Qiang Zhao, Ji-Dao Du
Summary: Cadmium and drought stress significantly inhibited the growth of adzuki bean seedling plants, with the combined stress showing the highest degree of inhibition. Cd+D treatment reduced Cd accumulation in adzuki bean seedling roots. The treatment altered antioxidant enzyme activities and cell cycle phases in the roots, leading to changes in reactive oxygen species content.
SCIENTIA HORTICULTURAE
(2024)
Article
Horticulture
Lucette Adet, Danae M. A. Rozendaal, Arthur Tapi, Pieter A. Zuidema, Philippe Vaast, Niels P. R. Anten
Summary: This study investigated the effects of water stress on cocoa trees and found that they are highly sensitive to water stress, with different genotypes exhibiting varied responses. Potassium application did not mitigate the negative effects of water stress. There was significant variation among genotypes in terms of leaf and crown traits, and positive interactions between genotype and irrigation were observed. These findings provide insights into the acclimation strategies of cocoa trees and can be useful for selecting drought-tolerant genotypes.
SCIENTIA HORTICULTURAE
(2024)
