RNA-Seq is not required to determine stable reference genes for qPCR normalization

Unbiased RNA-Seq-driven identification and validation of reference genes for quantitative RT-PCR analyses of pooled cancer exosomes

(2021) Yao Dai et al.   BMC GENOMICS

Transcriptional profiling of mouse peripheral nerves to the single-cell level to build a sciatic nerve ATlas (SNAT)

(2021) Daniel Gerber et al.   eLife

Alzheimer’s-associated PLCγ2 is a signaling node required for both TREM2 function and the inflammatory response in human microglia

(2020) Benjamin J. Andreone et al.   NATURE NEUROSCIENCE

Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of Alzheimer’s disease

(2020) Amanda McQuade et al.   Nature Communications

Identification of stable senescence-associated reference genes

(2019) Alejandra Hernandez-Segura et al.   AGING CELL

Optimal use of statistical methods to validate reference gene stability in longitudinal studies

(2019) Venkat Krishnan Sundaram et al.   PLoS One

Recurrent functional misinterpretation of RNA-seq data caused by sample-specific gene length bias

(2019) Shir Mandelboum et al.   PLOS BIOLOGY

GPR56/ADGRG1 regulates development and maintenance of peripheral myelin

(2018) Sarah D. Ackerman et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Systematic Selection of Reference Genes for the Normalization of Circulating RNA Transcripts in Pregnant Women Based on RNA-Seq Data

(2017) Stephen Chim et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

RNA-seq reveals more consistent reference genes for gene expression studies in human non-melanoma skin cancers

(2017) Van L.T. Hoang et al.   PeerJ

A Highly Efficient Human Pluripotent Stem Cell Microglia Model Displays a Neuronal-Co-culture-Specific Expression Profile and Inflammatory Response

(2017) Walther Haenseler et al.   Stem Cell Reports

Use of RNA-seq data to identify and validate RT-qPCR reference genes for studying the tomato-Pseudomonas pathosystem

(2017) Marina A. Pombo et al.   Scientific Reports

Benchmarking of RNA-sequencing analysis workflows using whole-transcriptome RT-qPCR expression data

(2017) Celine Everaert et al.   Scientific Reports

Experimental validation of methods for differential gene expression analysis and sample pooling in RNA-seq

(2015) Anto P. Rajkumar et al.   BMC GENOMICS

Reference Gene Validation for RT-qPCR, a Note on Different Available Software Packages

(2015) Ward De Spiegelaere et al.   PLoS One

With Reference to Reference Genes: A Systematic Review of Endogenous Controls in Gene Expression Studies

(2015) Joanne R. Chapman et al.   PLoS One

Identification of reference genes for qRT-PCR in human lung squamous-cell carcinoma by RNA-Seq

(2014) Cheng Zhan et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Selection and evaluation of novel reference genes for quantitative reverse transcription PCR (qRT-PCR) based on genome and transcriptome data in Brassica napus L.

(2014) Hongli Yang et al.   GENE

RNA-seq differential expression studies: more sequence or more replication?

(2013) Yuwen Liu et al.   BIOINFORMATICS

Careful Selection of Reference Genes Is Required for Reliable Performance of RT-qPCR in Human Normal and Cancer Cell Lines

(2013) Francis Jacob et al.   PLoS One

Human housekeeping genes, revisited

(2013) Eli Eisenberg et al.   TRENDS IN GENETICS

Alternative expression analysis by RNA sequencing

(2010) Malachi Griffith et al.   NATURE METHODS

Unconstrained mining of transcript data reveals increased alternative splicing complexity in the human transcriptome

(2010) I. G. Mollet et al.   NUCLEIC ACIDS RESEARCH

Transcript length bias in RNA-seq data confounds systems biology

(2009) Alicia Oshlack et al.   Biology Direct

Mapping identifiers for the integration of genomic datasets with the R/Bioconductor package biomaRt

(2009) Steffen Durinck et al.   Nature Protocols

Selection of Reference Genes for Quantitative Real-time RT-PCR Studies in Mouse Brain

(2008) Enrica Boda et al.   JOURNAL OF MOLECULAR NEUROSCIENCE

Analyzing real-time PCR data by the comparative CT method

(2008) Thomas D Schmittgen et al.   Nature Protocols