Article
Cell Biology
Dmytro Puchkov, Paul Markus Mueller, Martin Lehmann, Claudia Matthaeus
Summary: This study describes a novel method combining super-resolution light microscopy and platinum replica transmission electron microscopy (CLEM-PREM) for analyzing plasma membrane sheets in terms of their structural details, curvature, and associated protein composition. The implementation of super-resolution stimulated emission depletion (STED) microscopy reduces sample preparation time and technical challenges for imaging and analysis. Additionally, scanning electron microscopy (SEM) imaging overcomes technical challenges associated with replica preparation and transfer on a TEM grid.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Chemistry, Physical
Nadine Eissmann, Uwe Muehle, Uwe Gaitzsch, Gunnar Walther, Thomas Weissgaerber, Bernd Kieback
Summary: The effect of titanium addition beyond the solubility limit was investigated to demonstrate the precipitation hardening possibility of the Cantor alloy CoCrFeMnNi. Following appropriate heat treatment, a significant increase in hardness was detected due to nanometer-sized precipitates identified through TEM analysis.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Cell Biology
Maryna Ivanchenko, Artur A. Indzhykulian, David P. Corey
Summary: Hair cells in the vertebrate inner ear have actin-filled protrusions called stereocilia which mediate mechanosensitivity. Hereditary deafness is often linked to abnormalities in stereocilia bundles, making them essential for understanding genetic hearing loss models. Electron microscopy techniques, such as TEM and SEM, are crucial for studying stereocilia morphology and protein localization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Nanoscience & Nanotechnology
Inbal Weisbord, Tamar Segal-Peretz
Summary: BCP is a model system for studying and utilizing self-assembly in soft matter. Electron microscopy is a leading method for characterizing the three-dimensional structure of BCPs. This article discusses the principles, strengths, weaknesses, and challenges of two main 3D EM methods: transmission EM tomography and slice and view scanning EM tomography. It also reviews current and new cutting-edge EM methods that have the potential to expand our understanding of BCPs in the future.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Baris Yavas, Cain J. Hung, Mingxuan Li, Rainer J. Hebert, S. Pamir Alpay, Mark Aindow
Summary: Laser glazing in a powder bed fusion instrument was used to evaluate the microstructure of an Al85Cu6Fe3Cr6 alloy, revealing the presence of I-phase quasicrystals, Al2Cu, and FCC Al. Laser tracks produced under different conditions showed a fine and uniform phase mixture with over 70% I-phase, a thin film of Al2Cu, and no cracking or macrosegregation. The reasons for the formation of I-phase instead of the previously reported D-phase in this alloy were discussed.
SCRIPTA MATERIALIA
(2023)
Article
Chemistry, Physical
M. A. Mazo, A. C. Caballero, J. Rubio
Summary: In this study, cheap, abundant, and easily-obtained carbon fillers such as graphite and carbon black were successfully incorporated into a silicon oxycarbide matrix to obtain dense crack-free bulk composites with enhanced electrical and thermal conductivities. Raman parameters were crucial for understanding the electronic and/or phonon transport in the composites.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Chemistry, Physical
Munmun Basak, Md Lutfor Rahman, Md Farid Ahmed, Bristy Biswas, Nahid Sharmin
Summary: Cobalt ferrite nanoparticles were synthesized using co-precipitation technique with different precipitating agents, affecting the structure and size distribution. Various analysis methods showed highly correlated results, with SSP method being the most compatible.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
Review
Chemistry, Multidisciplinary
Asia Matatyaho Ya'akobi, Yeshayahu Talmon
Summary: Cryo-TEM and cryo-SEM are widely used in the study of aqueous systems, but face technical challenges when applied to nonaqueous liquid systems. Innovations in instrumental developments have advanced cryo-EM methodologies, overcoming some of the limitations in imaging nonaqueous systems.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Article
Materials Science, Multidisciplinary
Thomas Willum Hansen, Marc Willinger
Summary: Catalysis is a highly complex phenomenon involving fundamental processes on multiple length scales. The understanding of the full-scale complexity of catalysis is still limited, and the experimental documentation of how atomic-scale processes influence long-range order in materials is lacking. Elucidating the link between atomic-scale structural dynamics and collective behavior could lead to a deeper understanding and further optimization of catalysts and processes.
Article
Biochemical Research Methods
Lucy M. Collinson, Carles Bosch, Anwen Bullen, Jemima J. Burden, Raffaella Carzaniga, Cheng Cheng, Michele C. Darrow, Georgina Fletcher, Errin Johnson, Kedar Narayan, Christopher J. Peddie, Martyn Winn, Charles Wood, Ardan Patwardhan, Gerard J. Kleywegt, Paul Verkade
Summary: Volume electron microscopy (vEM) is a set of techniques that can reveal the 3D structure of cells and tissues at depths of at least 1 micrometer. An emerging grassroots community effort is rapidly showcasing the impact of vEM technology in life sciences and clinical research.
Review
Green & Sustainable Science & Technology
Akmaral Agibayeva, Mert Guney, Ferhat Karaca, Aiganym Kumisbek, Jong Ryeol Kim, Egemen Avcu
Summary: This review aims to comprehensively assess the toxicity and composition of particle-bound pollutants, evaluate different methods for characterizing particles, and provide recommendations for enhancing human health risk assessment. The study found that the cellular response to particles is complex and requires a combination of morphological and chemical data for a comprehensive assessment. The recommended method for single-particle analysis is SEM-EDS, followed by dry or wet chemical analysis techniques for studying the bulk chemistry of particles. Using bioaccessible/soluble fractions can improve the accuracy of human health risk assessment.
Article
Biochemistry & Molecular Biology
Samridhi Pathak, Nayan Deori, Aditi Sharma, Shirisha Nagotu, Avinash Kale
Summary: Tuberculosis is a serious infectious disease that affects millions of people globally every year, especially in tropical countries like India. The treatment of tuberculosis using first-line drugs can lead to drug-resistant strains and side effects such as muscle wasting and malnutrition. This study investigates the effects of these major tuberculosis drugs on actin polymerization, which plays a central role in muscle contraction. Biophysical approaches, in vivo and in vitro experiments, and computational simulations were used to understand the mechanism of muscle wasting in tuberculosis. The findings provide valuable insights into the interaction between tuberculosis drugs and actin filaments.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemical Research Methods
Ruohua Shi, Keyan Bi, Kai Du, Lei Ma, Fang Fang, Lingyu Duan, Tingting Jiang, Tiejun Huang
Summary: Cell membrane segmentation in electron microscopy (EM) images is an important task. Current approaches perform well on low-resolution datasets but struggle with high-resolution datasets. Through eye movement and perceptual consistency experiments, we found that humans are more sensitive to membrane structure and can tolerate misalignment. Based on these findings, we propose a computational framework that incorporates human perception characteristics, including the perceptual Hausdorff distance (PHD) evaluation metric and a PHD-guided segmentation network (PS-Net).
Article
Chemistry, Applied
Vivek K. Bajpai, InWha Park, Imran Khan, Fanar Hamad Alshammari, Pradeep Kumar, Lei Chen, MinKyun Na, Yun Suk Huh, Young-Kyu Han, Shruti Shukla
Summary: The protoberberine alkaloid THBA showed significant antioxidant potential and antimicrobial activity against Bacillus cereus, disrupting the membrane integrity and inhibiting biofilm formation. It also reduced ROS levels in fibroblast cells and decreased CFU counts in raw rice, suggesting its potential as a natural antioxidant and antimicrobial agent.
Article
Chemistry, Physical
Maxim Ozerov, Vitaly Sokolovsky, Sergey Nadezhdin, Ekaterina Zubareva, Nadezhda Zherebtsova, Nikita Stepanov, Lujun Huang, Sergey Zherebtsov
Summary: The TiZrNb metal-matrix composites reinforced by in-situ formed borides were produced using different amounts of TiB2. The composites exhibited varying microstructures and mechanical properties with the increase in boride content. Load transfer strengthening was found to be the main contributor to the overall strength of the composite. The biocompatibility evaluation suggested a decrease in the proliferation rate of mesenchymal stem cells with an increase in boride content.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Article
Materials Science, Multidisciplinary
Vikas Mittal, Tehsin Akhtar, Nadejda Matsko
MACROMOLECULAR MATERIALS AND ENGINEERING
(2015)
Article
Materials Science, Multidisciplinary
Vikas Mittal, Ali U. Chaudhry, Nadejda B. Matsko
PHILOSOPHICAL MAGAZINE
(2016)
Article
Materials Science, Composites
Fakhruddin Patwary, Nadejda Matsko, Vikas Mittal
POLYMER COMPOSITES
(2018)
Article
Pharmacology & Pharmacy
Martin Wolf, Victoria Klang, Maria Halper, Constanze Stix, Thomas Heuser, Harald Kotisch, Claudia Valenta
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2017)
Article
Pharmacology & Pharmacy
Lisa Binder, Eva Maria Kulovits, Romana Petz, Johanna Ruthofer, Dieter Baurecht, Victoria Klang, Claudia Valenta
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2018)
Article
Pharmacology & Pharmacy
Martin Wolf, Florian Reiter, Thomas Heuser, Harald Kotisch, Victoria Klang, Claudia Valenta
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2018)
Article
Chemistry, Physical
Vikas Mittal, Tehsin Akhtar, Gisha Luckachan, Nadejda Matsko
COLLOID AND POLYMER SCIENCE
(2015)
Article
Pharmacology & Pharmacy
Martin Wolf, Victoria Klang, Tatjana Stojcic, Carola Fuchs, Michael Wolzt, Claudia Valenta
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2018)
Article
Materials Science, Composites
Vikas Mittal, Nadejda Matsko
JOURNAL OF THERMOPLASTIC COMPOSITE MATERIALS
(2014)
Article
Polymer Science
Vikas Mittal, Gisha Elizabeth Luckachan, Nadejda B. Matsko
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2014)
Article
Materials Science, Multidisciplinary
Nadejda B. Matsko, Franz P. Schmidt, Ilse Letofsky-Papst, Artem Rudenko, Vikas Mittal
MICROSCOPY AND MICROANALYSIS
(2014)
Article
Engineering, Chemical
Vikas Mittal, Gisha E. Luckachan, Boril Chernev, Nadejda B. Matsko
POLYMER ENGINEERING AND SCIENCE
(2015)
Article
Chemistry, Physical
N. B. Matsko, V. Mittal
Article
Dermatology
Astrid Pany, Victoria Klang, Marion Brunner, Johanna Ruthofer, Elisabeth Schwarz, Claudia Valenta
SKIN PHARMACOLOGY AND PHYSIOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Sebastijan Kovacic, Nadejda B. Matsko, Gregor Ferk, Christian Slugovc
ACTA CHIMICA SLOVENICA
(2014)
Article
Microscopy
Shengli Zhang, Ying Wang, Bowei Wang, Yi Zeng, Jiani Li, Xingyue Wang, Cuihua Hu, Zhankun Weng, Zuobin Wang
Summary: Curcumin was found to have effects on the activity, migration, and mitochondrial membrane potential of malignant hepatocytes and their mitochondria. The findings from both optical microscopy and atomic force microscopy studies confirm these effects on cell morphology and biomechanics.
Article
Microscopy
Rutika Sansaria, Krishanu Dey Das, Alwin Poulose
Summary: This paper presents a method for quantifying Golgi dispersion images and classifying them using machine learning models. By applying automated image processing and segmentation, extracting features, and using different classifiers, accurate classification of Golgi images is achieved.
Article
Microscopy
Marjorie da Silva, Cintia Eleonora Lopes Justino, Fernando Henrique Carnimeo, Fernando Barbosa Noll, Johan Billen
Summary: This article describes the secretory glands in females of two species of Myzinum and discusses their homology with other glands in the Hymenoptera order. The study finds that the three glands, instead of the commonly believed two, are present in Myzinum species. However, the limited research on the structure, function, and evolution of female accessory glands in Hymenoptera prevents conclusive statements.
Article
Microscopy
Ping Hao, Wen Yin, Xi Chen, Shuangshuang Qin, Yue Yu, Yuan Yuan, Xiaoyu Quan, Bing Hu, Shouhai Chen, Yi Wu
Summary: This study confirmed the secretion of nano-scale exosomes-multivesicular bodies and the interaction of high endothelial cells (HECs) with lysosomes and mitochondria in the small intestine of laying birds. Immunohistochemistry and immunofluorescence results showed the strong immunoreactivity of exosome-related proteins and autophagic-related proteins in the lamina propria of the small intestine. These findings reveal an innovative communication method among HECs in the small intestine.
Article
Microscopy
Stefan Talu, Robert S. Matos, Henrique Duarte da Fonseca Filho, Daniela Predoi, Simona Liliana Iconaru, Carmen Steluta Ciobanu, Liliana Ghegoiu
Summary: This study developed composite layers based on magnesium-doped hydroxyapatite and chitosan, and demonstrated their good biocompatibility and antifungal effects against osteosarcoma cells. The attachment and growth of the cells on the composite layers were promoted, while the growth of Candida albicans was inhibited. The morphological analysis revealed complex 3D patterns on the cancer cell surfaces on the magnesium-containing composite layers.
