Article
Plant Sciences
Florence Ngo Ngwe, Sonja Siljak-Yakovlev
Summary: We studied the sex chromosomes of Dioscorea dumetorum in Cameroon and found that one pair of chromosomes is sex chromosomes. This research contributes to our understanding of the sex determination mechanism in D. dumetorum (standard sex-determining XX/XY system).
Article
Agriculture, Dairy & Animal Science
Marcello Mezzasalma, Elvira Brunelli, Gaetano Odierna, Fabio Maria Guarino
Summary: The study provides the first karyotype description of eight Uroplatus species, revealing chromosome diversity in terms of chromosome number, heterochromatin composition, and NORs localization. Chromosome reduction is proposed to have occurred through translocations of microchromosomes on larger chromosomes, with independent sex chromosome diversification in different genera.
Article
Genetics & Heredity
Kevin Santos da Silva, Larissa Glugoski, Marcelo Ricardo Vicari, Augusto Cesar Paes de Souza, Renata Coelho Rodrigues Noronha, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi
Summary: The Ancistrus genus demonstrates extensive chromosomal diversity, including the occurrence of heteromorphic sex chromosomes and unstable repetitive sequences. These findings provide important insights into the evolution and genetic mechanisms of the Ancistrus genus.
FRONTIERS IN GENETICS
(2022)
Article
Zoology
Leandro Ranucci, Carlos A. Fernandes, Luciana A. Borin-Carvalho, Isabel C. Martins-Santos, Ana L. B. de Portela-Castro
Summary: This study presents the first cytogenetic data of two allopatric populations of Moenkhausia bonita and one of M. forestii, revealing differences in chromosome number, karyotype formula, and B chromosomes between the two species. These distinctions may contribute as a reference in taxonomic studies of this genus, providing insights into the evolutionary and cytotaxonomic aspects.
NEOTROPICAL ICHTHYOLOGY
(2021)
Article
Multidisciplinary Sciences
Rosio G. Schneider, Juan M. Ferro, Ivana N. Reinko, Juan M. Boeris, Dario E. Cardozo, Diego Baldo
Summary: Cytogenetic analyses on Leptodactylus bufonius in Argentina revealed a karyotype of 2n = 2x = 22 and FN = 44, with secondary constrictions in chromosome pair 8 and a sex-linked pericentromeric band in chromosome pair 4. The presence of heteromorphic XY sex chromosomes in L. bufonius is the second case within the genus Leptodactylus and the fifth within the family Leptodactylidae.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2021)
Article
Multidisciplinary Sciences
Sevgi Unal Karakus
Summary: This study examined the karyotypes and chromosomal banding characteristics of three endemic Gobio species from Anatolia, Turkey, revealing that all species had a diploid chromosome number of 2n = 50 and a karyotype composed of one uniarmed and 24 biarmed chromosomes. The C-positive heterochromatin was located on the pericentromeres of most chromosomes, with nucleolus organizer regions located terminally on the p arms of one submetacentric chromosome pair in all three species. No morphologically differentiated sex chromosomes were detected in the study.
IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A-SCIENCE
(2021)
Article
Cell Biology
Carlos Alexandre Fernandes, Margarida Maria de Rossi Vieira
Summary: This study presents the first cytogenetic description of A. ellisi, a species of Apteronotus found in the upper Paraguay River basin. The results show that A. ellisi has 52 chromosomes, including different types of chromosomes and the absence of heteromorphic sex chromosomes. The presence of NORs and heterochromatic regions can be used as cytogenetic markers for A. ellisi, and centric fusions/fissions appear to play important roles in the karyotype evolution and differentiation among Apteronotus species.
Article
Genetics & Heredity
Alexander Kostmann, Lukas Kratochvil, Michail Rovatsos
Summary: Scincoidea is a reptilian clade with diversity in sex-determining systems. It has been debated whether skinks have sex chromosomes variations or even lack of sex chromosomes. While ZZ/ZW sex chromosomes have been identified in some scincoidean lizards, they are poorly differentiated and often undetectable by cytogenetic methods. However, this study reports the presence of ZZ/ZW sex chromosomes in the plated lizard Zonosaurus madagascariensis, suggesting a different pattern of sex chromosome evolution in this species.
Article
Biochemistry & Molecular Biology
Diogo Cavalcanti Cabral-de-Mello, Frantisek Marec
Summary: Repetitive DNAs are a large portion of eukaryote genomes, posing challenges in genome assembly due to the similarity between these repeats. The FISH technique is powerful for physical mapping of sequences on chromosomes, but there is a need for a universal and affordable protocol. Testing a FISH protocol on various insects and arthropods showed high quality results, indicating its potential utility in studying the evolution and role of repetitive DNA in arthropod genomes.
MOLECULAR GENETICS AND GENOMICS
(2021)
Article
Zoology
Lucas Henrique Bonfim Souza, Bruno Cansancao Silva, Caroline Correia Costa, Antonio Domingos Brescovit, Matheus Pires Rinca, Ana Lucia Dias, Douglas Araujo
Summary: This study presents the first cytogenetic analysis of Deinopidae and reveals interpopulational variation in chromosomal and sex chromosome composition of Deinopis spiders, with some populations exhibiting chromosomal fusion events.
Article
Plant Sciences
Sudarat Khensuwan, Weerayuth Supiwong, Chatmongkon Suwannapoom, Phichaya Buasriyot, Sitthisak Jantarat, Weera Thongnetr, Nawarat Muanglen, Puntivar Kaewmad, Pasakorn Saenjundaeng, Kriengkrai Seetapan, Thomas Liehr, Alongklod Tanomtong
Summary: In this study, the karyotype and chromosomal characteristics of Hypsibarbus malcolmi and H. wetmorei were examined. The results showed differences in chromosome numbers, karyotypes, and chromosomal features between the two species. Additionally, using microsatellite probes, substantial genomic divergence was observed between them.
COMPARATIVE CYTOGENETICS
(2023)
Article
Agronomy
Kai Wang, Hui Zhang, Haris Khurshid, Ayman Esh, Caiwen Wu, Qinnan Wang, Nathalie Piperidis
Summary: This paper reviews the application and progress of cytogenetic techniques in the genus Saccharum, and discusses the future applications of cytogenetics in genomic studies and breeding of sugarcane and other plants with complex genomes.
Article
Biology
Bohuslav Janousek, Roman Gogela, Vaclav Bacovsky, Susanne S. Renner
Summary: This study provides valuable data on the development, grouping, and divergence of sex-linked genes in the chromosomes of the sisters Coccinia grandis and Coccinia schimperi, contributing to the field of academic research.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Tao Zhang, Guanqing Liu, Hainan Zhao, Guilherme T. Braz, Jiming Jiang
Summary: Oligonucleotide (oligo)-fluorescence in situ hybridization (FISH) has become a new generation of FISH technique in plant molecular cytogenetics research. Chorus2 software effectively selects single-copy oligos, removes repetitive elements, and allows the development of FISH probes from plant species without an assembled genome, broadening its application in molecular cytogenetics research.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Olga G. Silkova, Yulia N. Ivanova, Dina B. Loginova, Lilia A. Solovey, Elena A. Sycheva, Nadezhda I. Dubovets
Summary: This study observed karyotype reorganization in three groups of wheat-rye hybrids based on FISH and C-banding. Aberrations including aneuploidy and translocations were detected, with some plants showing competitiveness in forming bivalents. However, meiotic restitution did not contribute to fertility or increased ploidy in subsequent generations.
Article
Ecology
Fabio P. de Sa, Celio F. B. Haddad, Miranda M. Gray, Vanessa K. Verdade, Maria Tereza C. Thome, Miguel T. Rodrigues, Kelly R. Zamudio
Article
Evolutionary Biology
Thais H. Condez, Celio F. B. Haddad, Kelly R. Zamudio
BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY
(2020)
Article
Multidisciplinary Sciences
Carla S. Cassini, Pedro P. G. Taucce, Thiago R. de Carvalho, Antoine Fouquet, Mirco Sole, Celio F. B. Haddad, Paulo C. A. Garcia
Article
Biodiversity Conservation
Wesley J. Neely, Sasha E. Greenspan, Luisa P. Ribeiro, Tamilie Carvalho, Renato A. Martins, David Rodriguez, Jason R. Rohr, Celio F. B. Haddad, Luis Felipe Toledo, C. Guilherme Becker
BIOLOGICAL CONSERVATION
(2020)
Article
Evolutionary Biology
Boris L. Blotto, Mariana L. Lyra, Monica C. S. Cardoso, Miguel Trefaut Rodrigues, Iuri R. Dias, Euvaldo Marciano-Jr, Francisco Dal Vechio, Victor G. D. Orrico, Reuber A. Brandao, Clodoaldo Lopes de Assis, Amanda S. F. Lantyer-Silva, Mike G. Rutherford, Giussepe Gagliardi-Urrutia, Mirco Sole, Diego Baldo, Ivan Nunes, Rodrigo Cajade, Ambrosio Torres, Taran Grant, Karl-Heinz Jungfer, Helio R. da Silva, Celio F. B. Haddad, Julian Faivovich
Summary: The research on South American and West Indian Casque-headed Treefrogs revealed the diverse morphology and life histories of the species. A phylogenetic analysis was conducted, leading to the identification of new species and the suggestion of taxonomic work needed in certain clades. The study emphasized the nonmonophyly of some recognized species groups and highlighted the multiple independent origins of certain breeding behaviors.
Article
Cell Biology
Marcelo J. da Silva, Raquel Fogarin Destro, Thiago Gazoni, Hideki Narimatsu, Paulo S. Pereira dos Santos, Celio F. B. Haddad, Patricia P. Parise-Maltempi
CYTOGENETIC AND GENOME RESEARCH
(2020)
Article
Zoology
Pedro P. G. Taucce, Jamile S. Nascimento, Camila C. Trevisan, Felipe S. F. Leite, Diego J. Santana, Celio F. B. Haddad, Marcelo F. Napoli
JOURNAL OF HERPETOLOGY
(2020)
Article
Zoology
N. C. Pupin, F. Brusquetti, C. F. B. Haddad
JOURNAL OF ZOOLOGY
(2020)
Article
Ecology
Jose Wagner Ribeiro, Tadeu Siqueira, Graziella V. DiRenzo, Carolina Lambertini, Mariana L. Lyra, Luis Felipe Toledo, Celio F. B. Haddad, C. Guilherme Becker
Article
Biochemistry & Molecular Biology
Carla Martins Lopes, Delio Baeta, Alice Valentini, Mariana Lucio Lyra, Ariadne Fares Sabbag, Joao Luiz Gasparini, Tony Dejean, Celio Fernando Basptista Haddad, Kelly Raquel Zamudio
Summary: The study successfully used environmental DNA (eDNA) metabarcoding to detect DNA traces of 30 target amphibian species in Brazil, including four declined species, two locally disappeared species, and one species not seen since 1968. This highlights the potential of eDNA in biodiversity monitoring and conservation biology for evaluating the persistence and distribution of threatened species.
Article
Evolutionary Biology
Victor G. D. Orrico, Taran Grant, Julian Faivovich, Mauricio Rivera-Correa, Marco A. Rada, Mariana L. Lyra, Carla S. Cassini, Paula H. Valdujo, Walter E. Schargel, Denis J. Machado, Ward C. Wheeler, Cesar Barrio-Amoros, Daniel Loebmann, Jiri Moravec, Juliana Zina, Mirco Sole, Marcelo J. Sturaro, Pedro L. V. Peloso, Pablo Suarez, Celio F. B. Haddad
Summary: This study focused on the phylogeny of the hyline tribe Dendropsophini, confirming the monophyly of the tribe and the reciprocal monophyly of the genera Dendropsophus and Xenohyla. The research identified nine species groups within Dendropsophini, with some species groups showing paraphyletic relationships. The study also discussed the evolution of oviposition site selection, revealing multiple instances of independent evolution of terrestrial egg clutches in the evolutionary history of Dendropsophus.
Article
Zoology
Felipe Silva de Andrade, Isabelle Aquemi Haga, Mariana Lucio Lyra, Thiago Gazoni, Victor Zaracho, Celio Fernando Baptista Haddad, Luis Felipe Toledo, Ariovaldo Antonio Giaretta
Summary: In recent years, there has been an increase in taxonomic knowledge of Pseudopaludicola, allowing for a better understanding of the geographic distributions of species within this genus. This study accessed new data to evaluate the geographic distribution of P. boliviana and several species within the P. saltica group, providing new records and insights.
STUDIES ON NEOTROPICAL FAUNA AND ENVIRONMENT
(2022)
Article
Zoology
Marcus Thadeu T. Santos, Rafael F. Magalhaes, Rodrico B. Ferreira, Stenio E. Vittorazzi, Iuri R. Dias, Felipe S. F. Leite, Luciana B. Lourenco, Fabricio R. Santos, Celio F. B. Haddad, Paulo C. A. Garcia
HERPETOLOGICAL MONOGRAPHS
(2020)
Article
Biodiversity Conservation
Camila Zornosa-Torres, Guilherme Augusto-Alves, Mariana L. Lyra, Jose Cassimiro da Silva Junior, Paulo C. A. Garcia, Felipe Leite, Vanessa Verdade, Miguel T. Rodrigues, Joao Luiz Gasparini, Celio F. B. Haddad, Luis Felipe Toledo
Article
Biochemistry & Molecular Biology
Luiza Rieder Cholak, Celio F. B. Haddad, Patricia P. Parise-Maltempi
GENETICS AND MOLECULAR BIOLOGY
(2020)
Article
Microscopy
Shengli Zhang, Ying Wang, Bowei Wang, Yi Zeng, Jiani Li, Xingyue Wang, Cuihua Hu, Zhankun Weng, Zuobin Wang
Summary: Curcumin was found to have effects on the activity, migration, and mitochondrial membrane potential of malignant hepatocytes and their mitochondria. The findings from both optical microscopy and atomic force microscopy studies confirm these effects on cell morphology and biomechanics.
Article
Microscopy
Rutika Sansaria, Krishanu Dey Das, Alwin Poulose
Summary: This paper presents a method for quantifying Golgi dispersion images and classifying them using machine learning models. By applying automated image processing and segmentation, extracting features, and using different classifiers, accurate classification of Golgi images is achieved.
Article
Microscopy
Marjorie da Silva, Cintia Eleonora Lopes Justino, Fernando Henrique Carnimeo, Fernando Barbosa Noll, Johan Billen
Summary: This article describes the secretory glands in females of two species of Myzinum and discusses their homology with other glands in the Hymenoptera order. The study finds that the three glands, instead of the commonly believed two, are present in Myzinum species. However, the limited research on the structure, function, and evolution of female accessory glands in Hymenoptera prevents conclusive statements.
Article
Microscopy
Ping Hao, Wen Yin, Xi Chen, Shuangshuang Qin, Yue Yu, Yuan Yuan, Xiaoyu Quan, Bing Hu, Shouhai Chen, Yi Wu
Summary: This study confirmed the secretion of nano-scale exosomes-multivesicular bodies and the interaction of high endothelial cells (HECs) with lysosomes and mitochondria in the small intestine of laying birds. Immunohistochemistry and immunofluorescence results showed the strong immunoreactivity of exosome-related proteins and autophagic-related proteins in the lamina propria of the small intestine. These findings reveal an innovative communication method among HECs in the small intestine.
Article
Microscopy
Stefan Talu, Robert S. Matos, Henrique Duarte da Fonseca Filho, Daniela Predoi, Simona Liliana Iconaru, Carmen Steluta Ciobanu, Liliana Ghegoiu
Summary: This study developed composite layers based on magnesium-doped hydroxyapatite and chitosan, and demonstrated their good biocompatibility and antifungal effects against osteosarcoma cells. The attachment and growth of the cells on the composite layers were promoted, while the growth of Candida albicans was inhibited. The morphological analysis revealed complex 3D patterns on the cancer cell surfaces on the magnesium-containing composite layers.