Article
Genetics & Heredity
Kevin Santos da Silva, Larissa Glugoski, Marcelo Ricardo Vicari, Augusto Cesar Paes de Souza, Renata Coelho Rodrigues Noronha, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi
Summary: The Ancistrus genus demonstrates extensive chromosomal diversity, including the occurrence of heteromorphic sex chromosomes and unstable repetitive sequences. These findings provide important insights into the evolution and genetic mechanisms of the Ancistrus genus.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Diogo Cavalcanti Cabral-de-Mello, Frantisek Marec
Summary: Repetitive DNAs are a large portion of eukaryote genomes, posing challenges in genome assembly due to the similarity between these repeats. The FISH technique is powerful for physical mapping of sequences on chromosomes, but there is a need for a universal and affordable protocol. Testing a FISH protocol on various insects and arthropods showed high quality results, indicating its potential utility in studying the evolution and role of repetitive DNA in arthropod genomes.
MOLECULAR GENETICS AND GENOMICS
(2021)
Article
Agronomy
Kai Wang, Hui Zhang, Haris Khurshid, Ayman Esh, Caiwen Wu, Qinnan Wang, Nathalie Piperidis
Summary: This paper reviews the application and progress of cytogenetic techniques in the genus Saccharum, and discusses the future applications of cytogenetics in genomic studies and breeding of sugarcane and other plants with complex genomes.
Article
Biology
Bohuslav Janousek, Roman Gogela, Vaclav Bacovsky, Susanne S. Renner
Summary: This study provides valuable data on the development, grouping, and divergence of sex-linked genes in the chromosomes of the sisters Coccinia grandis and Coccinia schimperi, contributing to the field of academic research.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Tao Zhang, Guanqing Liu, Hainan Zhao, Guilherme T. Braz, Jiming Jiang
Summary: Oligonucleotide (oligo)-fluorescence in situ hybridization (FISH) has become a new generation of FISH technique in plant molecular cytogenetics research. Chorus2 software effectively selects single-copy oligos, removes repetitive elements, and allows the development of FISH probes from plant species without an assembled genome, broadening its application in molecular cytogenetics research.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Zoology
Mauro Nirchio, Maria Cecilia Masache, Fabilene Gomes Paim, Marcelo de Bello Cioffi, Orlando Moreira Filho, Ramiro Barriga, Claudio Oliveira, Anna Rita Rossi
Summary: Parodontidae is a small group of Neotropical characiform fishes with three genera and 32 species. This study describes the karyotype of S. wagneri, revealing a diploid chromosome number of 2n = 54 and the presence of ZZ/ZW type sex chromosomes. The data suggest a conservative karyotype structure in Parodontidae and hint at an ancient origin of the sex chromosomes in this group of fishes.
NEOTROPICAL ICHTHYOLOGY
(2021)
Review
Genetics & Heredity
Vladimir E. Gokhman
Summary: This comprehensive review summarizes the main approaches, techniques, and results of chromosome studies in parasitic wasps. It discusses the distribution of chromosome numbers in this group, the use of karyotype analysis and morphometric study for identification and differentiation, and the detection of interspecific differences using banding and staining techniques. It also highlights the use of fluorescence in situ hybridization (FISH) for physical mapping and suggests potential for genome evolution studies using chromosome microdissection combined with FISH.
Article
Biotechnology & Applied Microbiology
Dariusz Kadluczka, Ewa Grzebelus
Summary: The study revealed the presence of CentDc repeats in the genomes of 26 Daucus species belonging to both Daucus I and II subclades, as well as in one closely related outgroup species. The number of chromosome pairs with CentDc signals varied among different Daucus taxa, but the centromeric localization was conserved. Detailed chromosome measurements in 16 accessions provided insights into the intra- and interspecific karyological relationships among them.
Review
Biochemistry & Molecular Biology
Lismeri Wuicik Merfort, Mateus de Oliveira Lisboa, Luciane Regina Cavalli, Carmem Maria Sales Bonfim
Summary: Fanconi Anemia is a genomic instability disease characterized by sensitivity to DNA cross-linking agents and chromosomal abnormalities. Diagnosis relies on cytogenetic analysis, which reveals chromosomal breaks induced by cross-linking agents. Understanding the genetic abnormalities and monitoring patients' prognosis is important for clinical management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Genetics & Heredity
Martina Johnson Pokorna, Radka Reifova
Summary: B chromosomes are additional chromosomes found in many eukaryotic organisms, believed to originate from rearrangements and duplications of standard chromosomes. They exhibit unstable inheritance in populations and are often associated with mechanisms like meiotic drive. Despite previously being considered nonessential, recent studies have shown that B chromosomes can play significant roles in processes such as sex determination and pathogenicity.
FRONTIERS IN GENETICS
(2021)
Article
Ecology
G. Gouvi, A. Gariou-Papalexiou, Antonios A. Augustinos, E. Drosopoulou, George Tsiamis, Kostas Bourtzis, A. Zacharopoulou
Summary: The availability of polytene chromosomes and the development of polytene chromosome maps have greatly facilitated genetic analysis in Diptera and understanding of chromosomal organization. By comparing polytene chromosomes of different tephritid species, chromosomal rearrangements, mainly inversions, have been identified, potentially serving as a useful diagnostic tool for genetic and taxonomic studies across closely and distantly related species. The cytogenetic analysis of Zeugodacus tau and Zeugodacus cucurbitae, along with in situ hybridization experiments, confirm the genetic closeness between these two species and support their current taxonomic placement within the Zeugodacus genus.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2022)
Review
Zoology
Robert B. Angus, David Sadilek, Fatma Shaarawi, Hayley Dollimore, Hsing-Che Liu, Matthias Seidel, Vit Sykora, Martin Fikacek
Summary: This study provides a comprehensive summary of karyotype data for various genera and species of water scavenger beetles, revealing that the majority of Hydrophilidae are diploid and sexually reproducing, with distinct sex chromosome patterns.
ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY
(2021)
Article
Oncology
Daniel Mettman, Azhar Saeed, Janna Shold, Raquele Laury, Andrew Ly, Irfan Khan, Shivani Golem, Mojtaba Olyaee, Maura O'Neil
Summary: The study found that UroVysion was the most sensitive method, with all methods having high specificity. Cytology, forceps biopsies, and fine needle aspiration were also proven to have certain sensitivity and specificity for detecting malignancy in pancreatobiliary strictures. The most effective approach to maintaining high sensitivity while preserving specificity was limiting malignant interpretation to cases with 5+ cells showing the same polysomic signal pattern and/or loss of one or both 9p21 signals.
Article
Genetics & Heredity
Alexander Kostmann, Lukas Kratochvil, Michail Rovatsos
Summary: Scincoidea is a reptilian clade with diversity in sex-determining systems. It has been debated whether skinks have sex chromosomes variations or even lack of sex chromosomes. While ZZ/ZW sex chromosomes have been identified in some scincoidean lizards, they are poorly differentiated and often undetectable by cytogenetic methods. However, this study reports the presence of ZZ/ZW sex chromosomes in the plated lizard Zonosaurus madagascariensis, suggesting a different pattern of sex chromosome evolution in this species.
Article
Developmental Biology
Halida Thanveer Asana Marican, Lucas W. H. Sun, Hongyuan Shen
Summary: This study describes a simple method for establishing well-separated metaphases from individual zebrafish embryos, providing a new approach for investigating DNA damage, chromosome aberrations, and genomic instability.
Article
Plant Sciences
Sukhonthip Ditcharoen, Sudarat Khensuwan, Kriengkrai Seetapan, Phongphan Soonthornvipat, Chatmongkon Suwannapoom, Krit Pinthong, Sampan Tongnunui, Marcelo de Bello Cioffi, Thomas Liehr, Alongklod Tanomtong, Weerayuth Supiwong
Summary: The first chromosomal analysis of Salween shovelhead catfish (Sperata acicularis) revealed a diploid chromosome number of 56 with a karyotype composed of different types of chromosomes and unidentified sex chromosomes. Additionally, the distribution patterns of microsatellite repeats differed on the chromosomes.