Article
Microbiology
Mira M. Shenouda, Ryan S. Noyce, Stephen Z. Lee, Jun Li Wang, Yi-Chan Lin, Nicole A. Favis, Megan A. Desaulniers, David H. Evans
Summary: Poxvirus genomes possess hairpin structures that play a crucial role in genome packaging and replication. Through mutant vaccinia virus construction and experimental testing, it was found that the hairpin structure, rather than its sequence, determines its function. Mismatches within the hairpin were shown to be critical for genome packaging, and the study also provided insights into the arrangement of VACV flip and flop telomeres.
Review
Immunology
Rakesh Kulkarni, Erik A. C. Wiemer, Wen Chang
Summary: Lipid rafts are important components of cell membranes and play crucial roles in cellular processes and signaling. Both pathogens and hosts utilize lipid rafts to survive and initiate antimicrobial responses. This review focuses on the enrichment of major vault protein in lipid rafts during vaccinia virus infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
James P. Cwick, Jonathan E. Owen, Irina Kochetkova, Kyle S. Hain, Nick Van Horssen, Matthew P. Taylor
Summary: Most viruses utilize superinfection exclusion (SIE) to conserve resources and control population dynamics. This study provides new insights into how alphaherpesviruses limit sequential infection through the undercharacterized gD-independent SIE, which differs between PRV and HSV-1.
MICROBIOLOGY SPECTRUM
(2022)
Article
Gastroenterology & Hepatology
Maika S. Deffieu, Camille M. H. Clement, Cristina M. Dorobantu, Emma Partiot, Yonis Bare, Orestis Faklaris, Benjamin Riviere, Nilda Vanesa Ayala-Nunez, Thomas F. Baumert, Philippe Ronde, Yves Mely, Vincent Lucansky, Raphael Gaudin
Summary: This study provides robust evidence that HCV particles interact with OCLN away from TJs and sheds mechanistic insights regarding the manipulation of transmembrane receptor localization by extracellular virus particles.
Review
Biochemistry & Molecular Biology
Simon Verdonck, Josephine Nemegeer, Peter Vandenabeele, Jonathan Maelfait
Summary: Cell death, including necroptosis and pyroptosis, is an important part of the antiviral immune response, eliminating virus-infected cells and promoting immune development through the release of inflammatory mediators. Viruses employ evasion mechanisms to suppress these cell death pathways and antagonize host immunity.
TRENDS IN MICROBIOLOGY
(2022)
Review
Virology
Pedro J. de Pablo, Carmen San Martin
Summary: Understanding the assembly and disassembly of adenoviruses is challenging due to their complexity. Cryoelectron microscopy and atomic force microscopy have provided insights into the structure and function of adenoviruses, particularly the roles of core proteins.
CURRENT OPINION IN VIROLOGY
(2022)
Article
Virology
Andrew Tak, Jeffrey L. Americo, Ulrike S. Diesterbeck, Bernard Moss
Summary: Experimental evolution showed adaptive mutations in viruses lacking the O3 gene, with variants containing F9L, L5R, and D8L mutations exhibiting higher virus titers. The F9L mutation appeared earlier in three independent passages than the L5R and D8L mutations. Further analysis revealed that the adaptive F9L mutants had increased infectivity, faster cell entry, and enhanced EFC assembly.
JOURNAL OF VIROLOGY
(2021)
Article
Cell Biology
Neha Gupta, Cristina Soriano-Ubeda, Paula Stein, Virginia Savy, Brian N. Papas, Goli Ardestani, Ingrid Carvacho, Dominique Alfandari, Carmen J. Williams, Rafael A. Fissore
Summary: TRPM7 is a crucial chanzyme for embryo development. It maintains magnesium (Mg2+) homeostasis, prevents oxidative stress, and promotes gene expression necessary for developmental progression and cell-lineage specification.
Article
Microbiology
Kevin Hervouet, Martin Ferrie, Maliki Ankavay, Claire Montpellier, Charline Camuzet, Virginie Alexandre, Aicha Dembele, Cecile Lecoeur, Arnold Thomas Foe, Peggy Bouquet, David Hot, Thibaut Vausselin, Jean-Michel Saliou, Sophie Salome-Desnoulez, Alexandre Vandeputte, Laurent Marsollier, Priscille Brodin, Marlene Dreux, Yves Rouille, Jean Dubuisson, Cecile-Marie Aliouat-Denis, Laurence Cocquerel
Summary: Hepatitis E virus (HEV) is a common cause of acute viral hepatitis worldwide and can become chronic in immunocompromised individuals. The ORF2 protein encoded by the HEV genome plays a crucial role in the virus lifecycle and interactions with the host. This study identified an Arginine-Rich Motif (ARM) in the N-terminal region of ORF2 that controls the fate and functions of different isoforms of ORF2. The ARM regulates nuclear translocation, signal peptide topology, glycosylation, and host cell membrane interactions of ORF2.
Article
Biochemistry & Molecular Biology
Sophie L. Winter, Gonen Golani, Fabio Lolicato, Melina Vallbracht, Keerthihan Thiyagarajah, Samy Sid Ahmed, Christian Luechtenborg, Oliver T. Fackler, Britta Bruegger, Thomas Hoenen, Walter Nickel, Ulrich S. Schwarz, Petr Chlanda
Summary: During Ebola virus entry into host cells, the virus disassembles and releases the nucleocapsid into the cytosol. This process is driven by the weakening of viral protein 40 (VP40) interactions caused by low endosomal pH. The disassembly of VP40 acts as a molecular switch coupling viral matrix uncoating to membrane fusion.
Article
Biology
Carina R. Buettner, Radovan Spurny, Tibor Fuezik, Pavel Plevka
Summary: CV-A6 has become the primary causative agent of hand, foot, and mouth disease worldwide. Unlike other enteroviruses, CV-A6 uses altered particles to infect cells. The structure of CV-A6 virion is similar to other enteroviruses and plays a crucial role in infection.
COMMUNICATIONS BIOLOGY
(2022)
Article
Plant Sciences
Eun-Ha Kim, Roshan Sharma Poudyal, Kyeong-Ryeol Lee, Hami Yu, Eunji Gi, Hyun Uk Kim
Summary: Recent studies have demonstrated the important role of CPSFL1/PITP7 in photoautotrophic growth and chloroplast vesicle formation. This study further investigated the functional roles of CPSFL1/PITP7 using A. thaliana mutants and found that PITP7 transcript levels were closely related to mutant phenotypes. The study also identified the binding affinity of PITP7 to PIPs and proposed a possible role for PITP7 in membrane trafficking of hydrophobic ligands.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Biochemistry & Molecular Biology
Quanquan Chen, Yingmei Guo, Jie Zhang, Nannan Zheng, Jie Wang, Yan Liu, Jiawen Lu, Sihan Zhen, Xuemei Du, Li Li, Junjie Fu, Guoying Wang, Riliang Gu, Jianhua Wang, Yunjun Liu
Summary: Maize (Zea mays) kernel size is determined by numerous genes, and in this study, the role of RNA polymerases in kernel development was investigated. The Dek701 mutant exhibited delayed endosperm development but normal vegetative growth and flowering transition. The Dek701 gene encoded a common subunit to RNA polymerases I, II, and III, and its loss-of-function mutation affected the function of all three polymerases and altered gene transcription related to RNA biosynthesis, phytohormone response, and starch accumulation. Transcriptional regulation of Dek701 by the transcription factor Opaque2 was also observed, providing valuable insights into the regulatory network of endosperm development in maize.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Cell Biology
Panpan Wang, Xi Chen, Yuanyuan Wang, Congcong Jia, Xinyao Liu, Ying Wang, Haifeng Wu, Huaibin Cai, Han-Ming Shen, Weidong Le
Summary: Deficiency of VMP1 in midbrain dopaminergic neurons leads to neuronal loss and impaired autophagic flux, resulting in various cellular abnormalities that affect neuronal survival and axonal homeostasis.
CELL DEATH & DISEASE
(2021)
Article
Plant Sciences
Lisha Zhang, Yang Liu, Gang Wei, Ting Lei, Jingwen Wu, Lintao Zheng, Honglei Ma, Guanghua He, Nan Wang
Summary: The integrity of pollen wall structures is crucial for pollen development and maturity in rice. In this study, a male-sterile mutant pwa1 with defective pollen wall structure and sterile pollen was characterized. The gene LOC_Os01g55094, encoding a coiled-coil domain-containing protein, was identified as PWA1. PWA1 interacted with the transcription factor TIP2 and played a role in regulating the formation of pollen wall and mature pollen in rice.
Article
Clinical Neurology
Nicolas Dzamko, Amanda M. Gysbers, Rina Bandopadhyay, Marc F. Bolliger, Akiko Uchino, Ye Zhao, Masaki Takao, Sandrine Wauters, Wilma D. J. van de Berg, Junko Takahashi-Fujigasaki, R. Jeremy Nichols, Janice L. Holton, Shigeo Murayama, Glenda M. Halliday
MOVEMENT DISORDERS
(2017)
Correction
Genetics & Heredity
J. William Langston, Birgitt Schuele, Linda Rees, R. Jeremy Nichols, Carrolee Barlow
Article
Biochemical Research Methods
Matthew D. Greseth, Dominique C. Carter, Scott S. Terhune, Paula Traktman
MOLECULAR & CELLULAR PROTEOMICS
(2017)
Article
Virology
Maciej W. Czarnecki, Paula Traktman
Article
Biochemistry & Molecular Biology
Alexia F. Kalogeropulou, Jing Zhao, Marc F. Bolliger, Anna Memou, Shreya Narasimha, Tyler P. Molitor, William H. Wilson, Hardy J. Rideout, R. Jeremy Nichols
BIOCHEMICAL JOURNAL
(2018)
Article
Virology
Xiangzhi Meng, Thomas Kaever, Bo Yan, Paula Traktman, Dirk M. Zajonc, Bjoern Peters, Shane Crotty, Yan Xiang
Article
Virology
Leonardo C. de Oliveira, Bruno S. A. F. Brasil, Bethany Unger, Giliane S. Trindade, Jonatas S. Abrahao, Erna G. Kroon, Paula Traktman, Claudio A. Bonjardim
Article
Multidisciplinary Sciences
Aye M. Mon, A. Craig MacKinnon, Paula Traktman
Article
Clinical Neurology
Farzaneh Atashrazm, Deborah Hammond, Gayathri Perera, Marc F. Bolliger, Elie Matar, Glenda M. Halliday, Birgitt Schule, Simon J. G. Lewis, R. Jeremy Nichols, Nicolas Dzamko
MOVEMENT DISORDERS
(2019)
Article
Biochemistry & Molecular Biology
Chun-Xiang Wu, Jingling Liao, Yangshin Park, Xylena Reed, Victoria A. Engel, Neo C. Hoang, Yuichiro Takagi, Steven M. Johnson, Mu Wang, Mark Federici, R. Jeremy Nichols, Ruslan Sanishvili, Mark R. Cookson, Quyen Q. Hoang
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Virology
Conor W. Templeton, Paula Traktman
Summary: Poxviruses remain of significant interest because of their continuing clinical relevance, their utility for the development of vaccines and oncolytic therapies, and their illustration of fundamental principles of viral replication and virus/cell interactions. These viruses are unique in that they replicate exclusively in the cytoplasm of infected mammalian cells, providing novel challenges for DNA viruses.
JOURNAL OF VIROLOGY
(2022)
Article
Biology
Jui-Tung Liu, Caren Doueiry, Yu-lin Jiang, Josef Blaszkiewicz, Mary Paige Lamprecht, James A. A. Heslop, Yuri K. K. Peterson, Juliana Debrito Carten, Paula Traktman, Yang Yuan, Salman R. R. Khetani, Waleed O. O. Twal, Stephen A. A. Duncan
Summary: iPSC-derived hepatocytes were used to identify small molecule inhibitors of apoB secretion without causing steatosis. These inhibitors show effectiveness in reducing apoB secretion in cultured hepatocytes and humanized livers in mice. This research provides a potential solution for treating FH patients with hoFH mutations, with the identified small molecules having a distinct chemical structure from existing cholesterol lowering drugs.
COMMUNICATIONS BIOLOGY
(2023)
Article
Virology
Juliana Debrito Carten, Matthew Greseth, Paula Traktman
Summary: An enduring mystery in poxvirology is the mechanism by which virion morphogenesis is accomplished. A30.5 and L2 are two small regulatory proteins that are essential for this process. Previous studies have shown that vaccinia A30.5 and L2 localize to the ER and interact during infection, but how they facilitate morphogenesis is unknown. To interrogate the relationship between A30.5 and L2, we generated inducible complementing cell lines (CV1-HA-L2; CV1-3xFLAG-A30.5) and deletion viruses (v Delta L2; v Delta A30.5). Loss of either protein resulted in a block in morphogenesis and a significant (> 100-fold) decrease in infectious viral yield.
JOURNAL OF VIROLOGY
(2022)
Article
Clinical Neurology
Ye Zhao, Gayathri Perera, Junko Takahashi-Fujigasaki, Deborah C. Mash, Jean Paul G. Vonsattel, Akiko Uchino, Kazuko Hasegawa, R. Jeremy Nichols, Janice L. Holton, Shigeo Murayama, Nicolas Dzamko, Glenda M. Halliday
Article
Cell & Tissue Engineering
Max A. Cayo, Sunil K. Mallanna, Francesca Di Furio, Ran Jing, Lauren B. Tolliver, Matthew Bures, Amanda Urick, Fallon K. Noto, Evanthia E. Pashos, Matthew D. Greseth, Maciej Czarnecki, Paula Traktman, Wenli Yang, Edward E. Morrisey, Markus Grompe, Daniel J. Rader, Stephen A. Duncan