Article
Chemistry, Applied
Zhaobin Guo, Cheng Chen, Guoyuan Ma, Qunli Yu, Li Zhang
Summary: Water distribution has a significant impact on meat quality, with proteins playing a crucial role in this process. Proton NMR was used to detect water distribution and its relation to protein properties. Three populations of water were identified: bound water, immobilized water, and free water. The changes in these water populations were correlated with water-holding capacity in aging muscles. Cattle exhibited higher water-holding capacity compared to yak. Additionally, postmortem aging affected muscle protein oxidation, denaturation, and degradation, which in turn influenced water distribution and retention.
Article
Medicine, Legal
Alessio Battistini, Daniele Capitanio, Paolo Bailo, Manuela Moriggi, Stefano Tambuzzi, Cecilia Gelfi, Andrea Piccinini
Summary: In this study, skeletal muscle tissue from pigs was degraded at different temperatures to identify proteins useful for postmortem interval estimation. PDLIM7, TPM1, and ATP2A2 were validated as potential markers for PMI assessment. The use of mass spectrometry analysis increased the number of identified protein species, expanding the panel for PMI evaluation.
FORENSIC SCIENCE INTERNATIONAL
(2023)
Article
Medicine, Legal
J. Geissenberger, B. Ehrenfellner, F. C. Monticelli, Stefan Pittner, Peter Steinbacher
Summary: The estimation of postmortem interval is crucial in forensic work, and muscle protein degradation analysis has shown potential in providing key markers for time constraints. Research indicates that using dismembered body parts as proxies for muscle protein analysis is effective in investigating influencing factors and establishing standardized models.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2021)
Article
Medicine, Legal
Stefan Pittner, Veronika Merold, Sven Anders, Larissa Lohner, Jens Amendt, Miriam Klinger, Roland Hausmann, Steffen Kissling, Fabio Monticelli, Janine Geissenberger, Angela Zissler, Peter Steinbacher
Summary: This study aimed to establish an easily replicable standardized procedure for sampling and processing of postmortem protein degradation, and investigate its reliability and limitations for routine application. The practical application was simulated by collecting tissue samples from three European forensic institutes and analyzing them according to the established protocol.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2022)
Article
Food Science & Technology
Hong Xiao, Jiao Yu, Linlu Song, Mengyue Hu, Hong Guo, Yong Xue, Changhu Xue
Summary: This study investigates the reasons behind the firmness of fish flesh and the easy separation of fish flesh after curing and fermentation. The results suggest that the increase in hardness and chewiness, along with changes in protein and muscle tissue structure, contribute to the firmness and easy separation of fermented sea bass meat. Pressure and curing dehydration play a key role in these changes.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Medicine, Legal
Janine Geissenberger, S. Pittner, B. Ehrenfellner, L. Jakob, W. Stoiber, F. C. Monticelli, P. Steinbacher
Summary: Recent research has shown that analyzing the degradation of postmortem muscle proteins can help determine the time since death in forensic investigations. This study used frozen and thawed pig limbs to investigate the effects of freezing and thawing on protein degradation and concluded that freezing and thawing has no significant impact on the degradation behavior.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Yayuan Yang, Jieyuan Yang, Jibing Ma, Qunli Yu, Ling Han
Summary: The study identified differentially expressed proteins associated with energy metabolism and tenderness in yak muscle tissue during postmortem aging. The proteins were categorized into cell structural, glycogen metabolic, energy reserve metabolic, and cellular polysaccharide metabolic proteins, with functions related to carbon metabolism, glycolysis, and amino acid biosynthesis. This research provides valuable insights into the molecular processes of energy metabolism in yak muscle tissue for future investigations.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Ya He, Haodong Yu, Ziyi Zhang, Jinying Zhang, Shengchao Kang, Xuezhen Zhang
Summary: This study investigated the effects of chronic hypoxia on the growth performance, antioxidant capacity, and protein turnover of largemouth bass. The results showed that chronic hypoxia decreased the growth performance, increased antioxidant capacity, and caused muscle atrophy due to altered protein turnover. Maintaining dissolved oxygen concentration above 60% DO saturation is suggested for better fish growth in aquaculture.
Article
Chemistry, Applied
Huan Yu, Jing Xie
Summary: The use of orthogonal ultrasonic wave in the freezing process of fish can effectively improve the quality by enhancing the utilization rate of ultrasonic. SEM images showed that the dual frequency orthogonal ultrasonic assisted freezing group (DOUAF-40 (H) 20 (V)) had more uniform and dense muscle tissue. Additionally, this group reduced the oxidation of myofibrin and duration of lipid oxidation in fish.
Article
Chemistry, Applied
Xueyuan Bai, Feng Yin, Ang Ru, Wei Tian, Jiahui Li, Guiyan Zhang, Qingwen Chen, Rong Chai, Kang Xiao, Chaozhi Zhu, Gaiming Zhao
Summary: There are correlations between myofiber types and postmortem redox characteristics in yak muscles. Different muscles have specific myofiber type compositions and redox characteristics. Type I myofiber is positively correlated with oxidation and antioxidative activity, while negatively correlated with lipid peroxidation and lactic dehydrogenase activity. Different processing methods are required for different muscles to prevent meat oxidation and produce standardized products.
Article
Environmental Sciences
Tania Charette, Maikel Rosabal, Marc Amyot
Summary: The study identified associations between metals and muscle lipids and proteins, which could help explain changes in bioaccumulation patterns within fish and individuals, with potential implications for fish toxicology and human consumption guidelines.
Article
Chemistry, Applied
Man-Man Yu, Ying-Chen Fan, Xin-Ran Zhang, De-Yang Li, Yu-Xin Liu, Da-Yong Zhou, Bei-Wei Zhu
Summary: Quantitative proteomic analysis revealed that texture changes in abalone muscles during boiling were caused by protein oxidation, denaturation, aggregation, and degradation, with the degradation of structural proteins leading to decreases in shear force and hardness.
Article
Agriculture, Multidisciplinary
Xin Yang, Weiqing Lan, XinYu Zhao, Ai Lang, Jing Xie
Summary: Chitosan-grafted chlorogenic acid (CS-g-CA) can protect the activity of antioxidant enzymes and inhibit lipid oxidation by slowing down the production of lipid oxidation products. It also delays protein oxidation by inhibiting oxidation product generation and stabilizing protein structure. It could be used as a promising preservative for seafood.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Fisheries
Anusara Kwankijudomkul, Ha Thanh Dong, Siwaporn Longyant, Paisarn Sithigorngul, Pongsak Khunrae, Triwit Rattanarojpong, Saengchan Senapin
Summary: A unique protein, HP33, was discovered in the Vibrio harveyi strain causing SDMND in Asian sea bass, which may serve as a potential target for developing vaccines and immunostimulants for SDMND in Asian sea bass.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Cell Biology
Michael L. Rossetti, Robert J. Tomko, Bradley S. Gordon
Summary: The study revealed that hypogonadism contributes to limb skeletal muscle atrophy by increasing rates of muscle protein breakdown and raising markers of the autophagy pathway. Various regulatory signals, such as ULK1 phosphorylation, JNK activation, and p53-regulated gene expression, were found to play a role in this process following androgen depletion. These novel changes shed light on the mechanisms involving autophagy in limb skeletal muscle after androgen deprivation.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Immunology
Silvia Torrecillas, Genciana Terova, Alex Makol, Antonio Serradell, Victoria Valdenegro-Vega, Marisol Izquierdo, Felix Acosta, Daniel Montero
Summary: The study aimed to evaluate the effects of plant-based oligosaccharides and phytogenic feed additives on the oxidative stress status and mucosal health of the gills of juvenile European sea bass. Results showed that these additives can protect the gill epithelia of sea bass from oxidative stress by modulating the expression of oxidative enzyme-related genes and reducing the density of PCNA+ cells in the gills.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Environmental Sciences
Federico Moroni, Fernando Naya-Catala, M. Carla Piazzon, Simona Rimoldi, Josep Calduch-Giner, Alberto Giardini, Ines Martinez, Fabio Brambilla, Jaume Perez-Sanchez, Genciana Terova
Summary: The addition of nisin-producing Lactococcus lactis in gilthead sea bream had effects on growth performance, transcriptional response, and microbiota, without colonization in the host's intestinal mucosa. The probiotic in the diets modulated the gut microbiota of fish, leading to host modifications without necessarily colonizing the intestine mucosa.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Parasitology
Grazia Biosa, Piero Bonelli, Salvatore Pisanu, Stefania Ghisaura, Cinzia Santucciu, Angela Peruzzu, Giovanni Garippa, Sergio Uzzau, Giovanna Masala, Daniela Pagnozzi
Summary: The proteome of hydatid cyst fluids of Echinococcus granulosus and other ovine parasites were analyzed, and potential diagnostic biomarkers for cystic echinococcosis (CE) were identified, which can predict CE in sheep and differentiate between different types of parasites.
Review
Veterinary Sciences
Anna Giagu, Martina Penati, Sara Traini, Simone Dore, Maria Filippa Addis
Summary: Mastitis is a major disease in dairy farming, and its detection is crucial for effective treatment. Non-enzymatic milk proteins have been investigated as alternative biomarkers for mastitis, and immunoassay techniques have been developed for their detection. However, there is significant heterogeneity among studies, highlighting the need for standardized criteria.
VETERINARY RESEARCH COMMUNICATIONS
(2022)
Article
Immunology
M. Filippa Addis, Salvatore Pisanu, Valentina Monistero, Alessandra Gazzola, Martina Penati, Joel Filipe, Susanna Di Mauro, Paola Cremonesi, Bianca Castiglioni, Paolo Moroni, Daniela Pagnozzi, Sebastiana Tola, Renata Piccinini
Summary: This study characterized the secretome of Staphylococcus aureus strains belonging to two different genotypes. The results revealed that the secretome of each genotype differed significantly, with one genotype showing a propensity for immune evasion and chronicity, while the other genotype showed a tendency for cellular damage and inflammation. These findings highlight the importance of extracellular virulence factors in S. aureus pathogenesis and provide insights into the relationship between genotypes and epidemiological behavior.
Article
Veterinary Sciences
Elisa Azara, Carla Maria Longheu, Sonia Attene, Silvana Sanna, Marco Sale, Maria Filippa Addis, Sebastiana Tola
Summary: Considerable variation in biofilm formation ability was observed among NAS isolates from ovine and human samples, with S. epidermidis being the best biofilm producer.
BMC VETERINARY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Antonio Serradell, Daniel Montero, Alvaro Fernandez-Montero, Genciana Terova, Alex Makol, Victoria Valdenegro, Felix Acosta, Maria Soledad Izquierdo, Silvia Torrecillas
Summary: The study evaluates the potential of two functional additives (GMOS and PHYTO) as boosters of gill endogenous antioxidant capacity in European sea-bass. The results show that both additives attenuate fish stress response and improve antioxidant status, indicating their potential to reduce the negative effects of stress in European sea bass.
Article
Agriculture, Dairy & Animal Science
Giovanni S. Molinari, Michal Wojno, Genciana Terova, Macdonald Wick, Hayden Riley, Jeffrey T. Caminiti, Karolina Kwasek
Summary: The use of adult Largemouth Bass muscle hydrolysates as a protein source for larval fish did not improve their growth performance. Further research is needed to determine the optimal protein source and form to reduce reliance on live feed during the larval stage of Largemouth Bass.
Article
Veterinary Sciences
Elisa Azara, Antonio Carlo Foddai, Carla Maria Longheu, Maria Filippa Addis, Sebastiana Tola
Summary: Staphylococcus aureus is a common pathogen associated with mastitis in sheep, affecting both animal welfare and milk production. Prevention and control measures, including good farm management and biosecurity practices, are crucial. Vaccination can help in preventing and eradicating diseases.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Review
Agriculture, Dairy & Animal Science
Imam Hasan, Simona Rimoldi, Giulio Saroglia, Genciana Terova
Summary: Aquaculture is a rapidly growing industry, but the use of fishmeal as a feed ingredient is not sustainable in the long-term. Insect meals and probiotics have emerged as potential alternatives to fishmeal and have shown positive effects on fish growth and health.
Article
Agriculture, Dairy & Animal Science
Antonio Serradell, Daniel Montero, Genciana Terova, Simona Rimoldi, Alex Makol, Felix Acosta, Aline Bajek, Pierrick Haffray, Francois Allal, Silvia Torrecillas
Summary: Husbandry practices in aquaculture may cause stress and oxidative damage to fish tissues. Functional ingredients can reinforce the fish's antioxidant response and stress tolerance. Selective breeding strategies have also shown a correlation between fish growth and stress reactiveness, which is important for species domestication.
Article
Agriculture, Dairy & Animal Science
Elisabetta Antuofermo, Massimo Orioles, Claudio Murgia, Giovanni P. Burrai, Martina Penati, Chiara Gottardi, Marta Polinas, Donatella Volpatti, Marco Galeotti, Maria Filippa Addis
Summary: In recent years, fish research has made significant advancements, especially in aquaculture, the ornamental fish industry, and biomedical studies. Immunohistochemistry has become crucial in fish research, aiding in physiological and pathological investigations. However, there is a need for validating antibodies specifically for fish species to ensure accuracy and reliability in immunohistochemistry analyses. This study systematically evaluated the reactivity of commonly used antibodies on four fish species and emphasized the importance of species-specific antibodies and proper validation for accurate immunohistochemistry analyses in fish research.
Article
Veterinary Sciences
Nives Maria Rosa, Martina Penati, Sara Fusar-Poli, Maria Filippa Addis, Sebastiana Tola
Summary: This study used MALDI-TOF MS and gap PCR-RFLP techniques to identify bacteria causing intramammary infection in small ruminants. The results showed a high level of agreement between these two methods in identifying the most prevalent non-aureus staphylococci and streptococci. Therefore, gap PCR-RFLP can be a viable alternative for identification when MALDI-TOF MS is not available.
VETERINARY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Chiara Ceccotti, Ilaria Biasato, Laura Gasco, Christian Caimi, Sara Bellezza Oddon, Simona Rimoldi, Fabio Brambilla, Genciana Terova
Summary: In this study, a feeding trial was conducted with rainbow trout to evaluate the bioavailability and efficacy of five different forms of methionine. The results showed that different forms of methionine had no effects on growth performance but led to changes in the whole-body composition of the fish. Additionally, specific forms of methionine affected hepatic methionine metabolism through different molecular pathways.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)