Article
Chemistry, Analytical
Lanyu Liu, Qian Liu, Yan Wang, Yu Yuan, Feifan Xie
Summary: This study aimed to evaluate different combinations of polyanion-metal ion based solution system for phospholipids removal and elimination of matrix effects in human plasma. The optimized ZrOCl2-Ludox/DSS systems provided efficient phospholipids removal and adequate analyte recovery, and they also correctly eliminated ion suppression or enhancement of the non-polar and polar drugs.
ANALYTICA CHIMICA ACTA
(2023)
Review
Medical Laboratory Technology
Philippe Massonnet, Elodie Grifnee, Jordi Farre-Segura, Justine Demeuse, Loreen Huyghebaert, Thomas Dubrowski, Patrice Dufour, Matthieu Schoumacher, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: Immunocapture is a mature method for sample preparation in quantitation of peptides and proteins. This review provides an overview of clinical applications of immunocapture methods, including protocols with and without enzymatic digestion. The advantages and limitations of these approaches are discussed, along with challenges related to mass spectrometer selection. The potential of these techniques in proteomics-based clinical medicine is demonstrated, paving the way for personalized medicine.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Medical Laboratory Technology
Stephanie Rankin-Turner, Liam M. Heaney
Summary: Mass spectrometry (MS) has become a powerful and versatile tool in clinical laboratory, with advancements in biomarker detection, protein measurement, therapeutic drug monitoring, and automation leading to potential revolution in clinical testing.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemical Research Methods
Allison Brookhart, Mahika Arora, Michael McCullagh, Ian D. Wilson, Robert S. Plumb, Johannes P. C. Vissers, Nikunj Tanna
Summary: Mobile phase selection is crucial in LC-MS studies, affecting various aspects such as retention, selectivity, ionization, and detection limits. This study evaluated the effect of solvent composition on ESI response for 240 small molecular weight drugs. Surface area and surface charge-related features were found to be the main factors influencing ESI response. The composition of the mobile phase had a less significant impact, with some compounds showing a pH effect.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Runmin Yang, Jingjing Ma, Shu Zhang, Yu Zheng, Lusheng Wang, Daming Zhu
Summary: This study introduces a new storage format called mzMD for MS datasets and presents an algorithm to query this format for summarizing a given data window. Experimental results demonstrate the high speed and stability of mzMD in retrieving high-quality data window summaries.
Article
Biochemical Research Methods
Yang Tang, Michael Van Parys, Abigail Walker, Alexandra Drelick, Xiaorong Liang, Brian Dean, Liuxi Chen
Summary: In this study, a LC-MS/MS surrogate matrix assay was developed for accurate urea quantitation in various biological matrices. The assay demonstrated reliable and robust performance, meeting bioanalysis requirements for clinical studies. The potential of the assay for urea quantitation in other physiological matrices was also explored.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Chemistry, Multidisciplinary
Hao Gu, Guoxin Dai, Siyu Liu, Yanbing Zhai, Qian Xu, Hongqiang Wu, Wei Xu
Summary: Liquid chromatography tandem mass spectrometry (LC-MS/MS) is an important analytical technique in clinical diagnostics. A compact LC-MS instrument was developed, consisting of an assembled liquid chromatograph and a miniature ion trap mass spectrometer. The use of LC improved the mixture analysis capability and detection sensitivity of the miniature mass spectrometer. This compact LC-MS instrument shows potential for clinical diagnosis.
CHINESE CHEMICAL LETTERS
(2023)
Article
Marine & Freshwater Biology
Fernanda R. Jacinavicius, Vanessa Geraldes, Kelly Fernandes, Camila M. Crnkovic, Watson A. Gama, Ernani Pinto
Summary: This study tested the effects of different cyanobacterial strains on zebrafish larvae and analyzed their metabolomic profiles. The results showed that some strains caused morphological abnormalities, while others did not. Metabolomics also revealed the diverse compounds produced by cyanobacteria. The effects of cyanobacterial metabolites on living organisms and their associated risks are still poorly understood.
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Armaghan Shafaei, Veronica Vamathevan, Jessica Pandohee, Nathan G. Lawler, David Broadhurst, Mary C. Boyce
Summary: This study presents a method for determining short-chain fatty acids in serum using high-resolution mass spectrometry and gas chromatography to identify isomeric interferences, which are overcome through a combination of chromatographic separation and judicious choice of MS fragment ion. The LODs and LOQs of SCFAs using this method were in the range 1 to 7 ng mL(-1) and 3 to 19 ng mL(-1), with a recovery ranging from 94 to 114% over the three concentration ranges tested.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yuan-Chih Chen, Hsin-Yi Wu, Chih-Wei Chang, Pao-Chi Liao
Summary: Conjugation reactions play a critical role in human metabolism, but identifying conjugated metabolites is challenging. In this study, a strategy called PDMS2E-DIA was proposed for comprehensive profiling of the glucuronide-conjugated metabolome. It successfully identified a large number of glucuronide-conjugated metabolites in human urine samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Sakshi Gautam, Alireza Banazadeh, Byeong Gwan Cho, Mona Goli, Jieqiang Zhong, Yehia Mechref
Summary: Researchers successfully achieved efficient separation of glycan isomers using mesoporous graphitized carbon (MGC) material in a 1 cm-long column, showing reproducibility and stability in both capillary columns and packed nanospray emitters.
ANALYTICAL CHEMISTRY
(2021)
Article
Plant Sciences
Trevor B. Romsdahl, Jean-Christophe Cocuron, Mackenzie J. Pearson, Ana Paula Alonso, Kent D. Chapman
Summary: This study introduces a stepwise targeted lipidomics approach to characterize polar and non-polar lipid classes. By using hydrophilic interaction chromatography and reverse phase chromatography, changes among different lipid molecular species were found, and potential metabolic explanations were discussed.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
John Quinones, Rommy Diaz, Jorge F. Beltran, Lidiana Velazquez, David Cancino, Erwin Munoz, Patricio Dantagnan, Adrian Hernandez, Nestor Sepulveda, Jorge G. Farias
Summary: This study analyzed the effects of different feeds on the lipid composition of juvenile Oncorhynchus mykiss muscle and found that the inclusion of Brassica napus oil significantly increased certain lipid levels, while Durvillaea antarctica meal had a less significant impact on lipid variation.
Article
Plant Sciences
Ilaria Chiocchio, Nerea Perez Andres, Redouan Adam Anaia, Nicole M. van Dam, Fredd Vergara
Summary: There are qualitative and quantitative differences in the steroidal glycosides profile between primary and adventitious roots of Solanum dulcamara, which can be used to evaluate the bioactivity of these molecules in belowground ecosystems.
Article
Oncology
Jenny L. Pokorny, David Calligaris, Shiv K. Gupta, Dennis O. Iyekegbe, Dustin Mueller, Katrina K. Bakken, Brett L. Carlson, Mark A. Schroeder, Debra L. Evans, Zhenkun Lou, Paul A. Decker, Jeanette E. Eckel-Passow, Vincenzo Pucci, Bennett Ma, Stuart D. Shumway, William F. Elmquist, Nathalie Y. R. Agar, Jann N. Sarkaria
CLINICAL CANCER RESEARCH
(2015)
Article
Microbiology
Vincenzo Summa, Steven W. Ludmerer, John A. McCauley, Christine Fandozzi, Christine Burlein, Giuliano Claudio, Paul J. Coleman, Jillian M. DiMuzio, Marco Ferrara, Marcello Di Filippo, Adam T. Gates, Donald J. Graham, Steven Harper, Darla J. Hazuda, Carolyn McHale, Edith Monteagudo, Vincenzo Pucci, Michael Rowley, Michael T. Rudd, Aileen Soriano, Mark W. Stahlhut, Joseph P. Vacca, David B. Olsen, Nigel J. Liverton, Steven S. Carroll
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2012)
Article
Biochemistry & Molecular Biology
M. Emilia Di Francesco, Salvatore Avolio, Marco Pompei, Silvia Pesci, Edith Monteagudo, Vincenzo Pucci, Claudio Giuliano, Fabrizio Fiore, Michael Rowley, Vincenzo Summa
BIOORGANIC & MEDICINAL CHEMISTRY
(2012)
Article
Multidisciplinary Sciences
Robert N. Booher, Harold Hatch, Brian M. Dolinski, Thi Nguyen, Lauren Harmonay, Ali-Samer Al-Assaad, Mark Ayers, Michael Nebozhyn, Andrey Loboda, Heather A. Hirsch, Theresa Zhang, Bin Shi, Carrie E. Merkel, Minilik H. Angagaw, Yaolin Wang, Brian J. Long, Xianlu Q. Lennon, Nathan Miselis, Vincenzo Pucci, James W. Monahan, Junghoon Lee, Anna Georgieva Kondic, Eun Kyung Im, David Mauro, Rebecca Blanchard, Gary Gilliland, Stephen E. Fawell, Leigh Zawel, Alwin G. Schuller, Peter Strack
Article
Biochemical Research Methods
Kevin P. Bateman, Lucinda Cohen, Bart Emary, Vincenzo Pucci
Article
Biochemical Research Methods
Lauren Stevenson, Fabio Garofolo, Binodh DeSilva, Isabelle Dumont, Suzanne Martinez, Mario Rocci, Lakshmi Amaravadi, Margarete Brudny-Kloeppel, Adrien Musuku, Brian Booth, Catherine Dicaire, Laura Wright, Laurence Mayrand-Provencher, Mike Losauro, Dominique Gouty, Mark Arnold, Surendra Bansal, Sherri Dudal, Dawn Dufield, Jeff Duggan, Christopher Evans, Eric Fluhler, Stephanie Fraser, Boris Gorovits, Sam Haidar, Roger Hayes, Stacy Ho, Richard Houghton, Rafiqul Islam, Rand Jenkins, Noriko Katori, Surinder Kaur, Marian Kelley, Magnus Knutsson, Jean Lee, Hanlan Liu, Steve Lowes, Mark Ma, Alvydas Mikulskis, Heather Myler, Bob Nicholson, Timothy Olah, Eric Ormsby, Shefali Patel, Vincenzo Pucci, Chad Ray, Gary Schultz, Judy Shih, Ronald Shoup, Craig Simon, An Song, Joao Tavares Neto, Valerie Theobald, Theingi Thway, Jason Wakelin-Smith, Jian Wang, Laixin Wang, Jan Welink, Emma Whale, Eric Woolf, Raymond Xu
Article
Biochemical Research Methods
Yang Xu, Eric J. Woolf, Nancy G. B. Agrawal, Prajakti Kothare, Vincenzo Pucci, Kevin P. Bateman
Article
Multidisciplinary Sciences
Johnny Kopinja, Raquel S. Sevilla, Diane Levitan, David Dai, Amy Vanko, Edward Spooner, Chris Ware, Robert Forget, Kun Hu, Astrid Kral, Peter Spacciapoli, Richard Kennan, Lata Jayaraman, Vincenzo Pucci, Samanthi Perera, Weisheng Zhang, Christian Fischer, Michael H. Lam
SCIENTIFIC REPORTS
(2017)
Article
Chemistry, Medicinal
Ping Liu, Liping Wang, Byron G. DuBois, Vincent J. Colandrea, Rongqiang Liu, Jiaqiang Cai, Xiaoxing Du, Weiro Quan, William Morris, Jianwu Bai, Bimjhana Bishwokarma, Mangeng Cheng, Jennifer Piesvaux, Kallol Ray, Carla Alpert, Chi-Sung Chiu, Mark Zielstorff, Joseph M. Metzger, Liming Yang, Dennis Leung, Candice Alleyne, Stella H. Vincent, Vincenzo Pucci, Xiaofang Li, Alejandro Crespo, Dominique Stickens, Jeffrey J. Hale, Feroze Ujjainwalla, Christopher J. Sinz
ACS MEDICINAL CHEMISTRY LETTERS
(2018)
Review
Biochemical Research Methods
Padmanabhan Eangoor, Sharmistha Das, Vincenzo Pucci
Article
Oncology
Samanthi A. Perera, Johnny E. Kopinja, Yanhong Ma, Eric S. Muise, Jason Laskey, Kalyan Chakravarthy, Yiping Chen, Long Cui, Jeremy Presland, Manjiri Sathe, Sarah Javaid, Ellen C. Minnihan, Heidi M. Ferguson, Jennifer Piesvaux, Bo-Sheng Pan, Shuxia Zhao, Sharad K. Sharma, Hyun Chong Woo, Vincenzo Pucci, Ian Knemeyer, Saso Cemerski, Jared Cumming, B. Wesley Trotter, Archie Tse, Anuradha Khilnani, Sheila Ranganath, Brian J. Long, David Jonathan Bennett, George H. Addona
Summary: STING agonists can enhance anti-PD-1 immune profile, promote tumor control, and have potential therapeutic value for patients with aggressive tumors.
MOLECULAR CANCER THERAPEUTICS
(2022)
Review
Pharmacology & Pharmacy
Simone Esposito, Laura Orsatti, Vincenzo Pucci
Summary: This review discusses the evolution of technology and strategy in elucidating peptide subcutaneous catabolism. Modern bioanalytical technologies and bioinformatics platforms have enabled in silico, in vitro, and in vivo tools for characterising peptide catabolism and guiding the design of peptides with improved subcutaneous bioavailability. More predictive models are desired to improve the correlation between subcutaneous catabolism and other factors driving subcutaneous absorption.
Article
Chemistry, Medicinal
Esther Torrente, Valentina Fodale, Alina Ciammaichella, Federica Ferrigno, Jesus M. Ontoria, Simona Ponzi, Ilaria Rossetti, Alessio Sferrazza, Jerome Amaudrut, Antonino Missineo, Simone Esposito, Simone Palombo, Martina Nibbio, Mauro Cerretani, Monica Bisbocci, Antonella Cellucci, Annalise di Marco, Cristina Alli, Vincenzo Pucci, Carlo Toniatti, Alessia Petrocchi
Summary: Protein tyrosine phosphatase SHP2 acts as an oncogenic protein by regulating various cytokine receptors and receptor tyrosine kinase signaling pathways. Here, we present a new series of SHP2 allosteric inhibitors with an imidazopyrazine 6,5-fused heterocyclic system as the central scaffold, which exhibit strong enzymatic and cellular potency. Compound 8, a highly potent SHP2 allosteric inhibitor, was identified through structure-activity relationship studies, showing novel stabilizing interactions compared to known SHP2 inhibitors. Further optimization led to the discovery of analogue 10, which demonstrates excellent potency and promising pharmacokinetic properties in rodents.
ACS MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Medicinal
Esther Torrente, Valentina Fodale, Alina Ciammaichella, Federica Ferrigno, Jesus M. Ontoria, Simona Ponzi, Ilaria Rossetti, Alessio Sferrazza, Jerome Amaudrut, Antonino Missineo, Simone Esposito, Simone Palombo, Martina Nibbio, Mauro Cerretani, Monica Bisbocci, Antonella Cellucci, Annalise di Marco, Cristina Alli, Vincenzo Pucci, Carlo Toniatti, Alessia Petrocchi
Summary: Protein tyrosine phosphatase SHP2 is an oncogenic protein that regulates multiple signaling pathways. This study identified a new series of SHP2 allosteric inhibitors with a central scaffold of imidazopyrazine 6,5-fused heterocyclic system, showing good potency in enzyme and cellular assays. Compound 8, a highly potent SHP2 allosteric inhibitor, was identified through SAR studies and X-ray analyses revealed novel stabilizing interactions. Further optimization led to the discovery of analogue 10, which exhibits excellent potency and promising pharmacokinetic profile in rodents.
ACS MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Oncology
Yun Wang, Long Cui, Peter Georgiev, Latika Singh, Yanyan Zheng, Ying Yu, Jeff Grein, Chunsheng Zhang, Eric S. Muise, David L. Sloman, Heidi Ferguson, Hongshi Yu, Cristina St. Pierre, Pranal J. Dakle, Vincenzo Pucci, James Baker, Andrey Loboda, Doug Linn, Christopher Brynczka, Doug Wilson, Brian B. Haines, Brian Long, Richard Wnek, Svetlana Sadekova, Michael Rosenzweig, Andrew Haidle, Yongxin Han, Sheila H. Ranganath
Summary: The study demonstrates that the EP4 inhibitor MF-766 synergizes with anti-PD-1 therapy to enhance anti-tumor activity by modulating infiltration profiles of myeloid cells, NK cells, cDCs, and T-cells in the tumor microenvironment (TME).
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
