Article
Cell Biology
Pascale Monzo, Michele Crestani, Yuk Kien Chong, Andrea Ghisleni, Katharina Hennig, Qingsen Li, Nikolaos Kakogiannos, Monica Giannotta, Cristina Richichi, Tania Dini, Elisabetta Dejana, Paolo Maiuri, Martial Balland, Michael P. Sheetz, Giuliana Pelicci, Beng Ti Ang, Carol Tang, Nils C. Gauthier
Summary: The study found that invasive cells are more rigid and have higher mechanical properties, and the expression of FMN1 is associated with invasiveness. FMN1 also promotes motility in other cancer and normal cell lines, increasing fitness parameters.
DEVELOPMENTAL CELL
(2021)
Review
Immunology
Angeliki Datsi, Ruediger V. Sorg
Summary: Dendritic cell vaccination (DCV) is a promising immunotherapy for treating glioblastomas, with numerous trials showing potential but some controlled studies failing to detect a survival benefit. Further exploration is needed to fully understand the potential of this therapeutic approach.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Laura Lucia Trebucq, Georgina Alexandra Cardama, Pablo Lorenzano Menna, Diego Andres Golombek, Juan Jose Chiesa, Luciano Marpegan
Summary: The study revealed circadian modulation in the efficacy of 1A-116, likely through RAC1 pathway rhythmicity, suggesting that a chronopharmacological approach is a feasible strategy to improve glioblastoma treatment.
Article
Biochemistry & Molecular Biology
Kumi Sakoe, Norifumi Shioda, Tohru Matsuura
Summary: Spastin is a microtubule-severing AAA ATPase that plays important roles in cell division and neurogenesis, with mutations in the SPAST gene leading to neurodegenerative disorders. The two main isoforms of Spastin, M1 and M87, differ in their N-terminal amino acids and have alternative splicing variants. A newly identified NES region containing exon4 regulates the subcellular localization of Spastin in coordination with NLS controlled by phosphorylation state.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Safwan Alomari, Irma Zhang, Adrian Hernandez, Caitlin Y. Kraft, Divyaansh Raj, Jayanidhi Kedda, Betty Tyler
Summary: Glioblastoma is a common malignant brain tumor with poor prognosis, and there is a need for effective therapeutics. Drug repurposing is a potential strategy, with some drugs showing efficacy against GBM cell lines. However, the blood-brain barrier may limit their effectiveness.
Article
Multidisciplinary Sciences
Qing Guo, Shuai Shen, Gefei Guan, Chen Zhu, Cunyi Zou, Jingyuan Cao, Wen Cheng, Xiaoyan Xu, Juanhan Yu, Zhiguo Lin, Guoli Wang, Ling Chen, Peng Cheng, Anhua Wu
Summary: Glioblastoma (GBM) shares common signaling pathways involving TIM-3, an immune checkpoint, between tumor and non-tumor cells. The study reveals that TIM-3 in glioma cells not only regulates the malignant behaviors of glioma cells but also induces macrophage migration and transition to an anti-inflammatory/protumorigenic phenotype through the TIM-3/IL6 signal. Blocking this feedback loop may provide a novel therapeutic strategy for GBM.
Review
Chemistry, Multidisciplinary
Shuangqi Yu, Lijie Chen, Hongyu Xu, Shengrong Long, Jiazhi Jiang, Wei Wei, Xing Niu, Xiang Li
Summary: The diagnosis and treatment of glioblastoma face several obstacles, but combining multiple methods can provide benefits. Nanomaterials and drug combinations can improve treatment outcomes and reduce side effects. This review discusses the progress and challenges of glioblastoma diagnosis and treatment, as well as the potential of nanomedicine in this field.
FRONTIERS IN CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Alanah Varricchio, Sunita A. Ramesh, Andrea J. Yool
Summary: Glioblastoma multiforme is a leading cause of brain cancer-related deaths worldwide, with cells capable of infiltrating healthy brain tissue rapidly. Targeting cellular motility pathways may help slow tumor dispersal and provide a longer time window for frontline treatments. Expressions of Aquaporins (AQPs) and voltage-gated ion channels in GBM cells could be pharmacological targets to restrain cell migration, potentially enhancing therapeutic effectiveness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Jed de Ruiter Swain, Evdokia Michalopoulou, Evan K. Noch, Michael J. Lukey, Linda Van Aelst
Summary: This review discusses the mechanisms of intercellular metabolite exchange in the healthy brain and how it is exploited by glioblastoma for tumor growth and modulation of the tumor microenvironment. The authors highlight the potential of targeting metabolic dependencies as a therapeutic avenue against this aggressive brain cancer.
GENES & DEVELOPMENT
(2023)
Review
Pharmacology & Pharmacy
Nikhil Pandey, Pavlos Anastasiadis, Christine P. Carney, Pranjali P. Kanvinde, Graeme F. Woodworth, Jeffrey A. Winkles, Anthony J. Kim
Summary: This review focuses on drug delivery strategies and nanotherapeutic technologies targeting brain-invading cancer cells and highlights the importance of treatments for invasive cells.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Oncology
Ziwei Li, Xiangqi Meng, Pengfei Wu, Caijun Zha, Bo Han, Lulu Li, Nan Sun, Tengfei Qi, Jie Qin, Yangong Zhang, Kaifu Tian, Shupeng Li, Changxiao Yang, Lejia Ren, Jianguang Ming, Pandeng Wang, Yifei Song, Chuanlu Jiang, Jinquan Cai
Summary: This study found that lnc-TALC could be transmitted to tumor-associated macrophages (TAM) via exosomes, promoting M2 polarization and increasing resistance of brain tumors to TMZ chemotherapy. The results suggest that the interaction mediated by lnc-TALC between GBM cells and microglia can reduce the efficacy of chemotherapy, indicating potential combination therapy strategies to overcome drug resistance.
CANCER IMMUNOLOGY RESEARCH
(2021)
Review
Cell Biology
Emily E. S. Brettschneider, Masaki Terabe
Summary: Glioblastoma is an aggressive and deadly cancer, and immunotherapies have not made significant progress. NKT cells may play a key immunoregulatory role in glioblastoma, but their specific function is still unclear.
Article
Neurosciences
Siqi Xia, Huaijun Chen, Tianchi Tang
Summary: This study found that patients with brain tumors, gliomas, and glioblastomas have a lower risk of Alzheimer's disease (AD) compared to patients with other tumors. Glioblastoma was significantly associated with a lower risk of AD compared to other tumors.
JOURNAL OF ALZHEIMERS DISEASE
(2023)
Article
Multidisciplinary Sciences
Ken Miyaguchi, Hongqiang Wang, Keith L. Black, Stephen L. Shiao, Rongfu Wang, John S. Yu
Summary: Efficient activation of T cells with tumor-associated antigens (TAAs) to glioblastoma (GBM) stem cells was achieved through the use of three different antigen sources loaded onto dendritic cells (DCs). An activated T cell (ATC) protocol was developed and optimized for a phase I clinical trial to target GSCs. The synthetic peptide pool loading method showed significantly increased cytotoxicity towards target cells.
SCIENTIFIC REPORTS
(2023)
Article
Oncology
Melodie Davy, Laurie Genest, Christophe Legrand, Oceane Pelouin, Guillaume Froget, Vincent Castagne, Tristan Rupp
Summary: Grade 4 gliomas and glioblastomas are the most common and aggressive brain tumors. Temozolomide, the current standard treatment, has varying responses depending on the tumor type, while Fingolimod, an immunomodulatory drug, may need further evaluation for its potential activity. The study highlights the need for new therapies to improve survival and quality of life for glioblastoma patients.
Article
Microscopy
Nejc Umek, Jiri Janacek, Erika Cvetko, Ida Erzen
Summary: Thick tissue sections in skeletal muscle research may experience horizontal deformation, impacting study results significantly. Our study showed that thick skeletal muscle sections dilate horizontally and shrink in the Z direction, with the magnitude of horizontal dilation associated with Z direction shrinkage. Optimizing histological protocols to minimize Z-axis collapse is important for result integrity.
JOURNAL OF MICROSCOPY
(2021)
Review
Cell Biology
Roger Karlsson, Pavel Draber
Summary: The last two decades have seen a significant increase in cell biology data, especially in studies of the dynamic organization of the microfilament and microtubule systems in animal cells. Recent research has focused on the actin regulator profilin 1 as a control component of microfilament-microtubule cross-talk.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Martin Capek, Michaela Blazikova, Ivan Novotny, Helena Chmelova, David Svoboda, Barbora Radochova, Jiri Janacek, Ondrej Horvath
Summary: This article proposes using discrete wavelet transform (DWT) for filtering super-resolved microscopic images, addressing the issue of potential resolution loss with traditional filtration techniques. The newly developed plugin in the Fiji software package demonstrates the important applications of DWT in image processing.
IMAGE ANALYSIS & STEREOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Anze Jerman, Jiri Janacek, Ziga Snoj, Nejc Umek
Summary: This study utilized virtual reality technology for planning minimally invasive surgical approaches, using solid angle as a measure of size. The findings demonstrate the feasibility of evaluating and comparing different minimally invasive approaches using solid angle as a valid measure.
IMAGE ANALYSIS & STEREOLOGY
(2021)
Review
Oncology
Pavel Draber, Eduarda Draberova
Summary: The dysfunction of microtubule nucleation in cancer cells alters the cytoskeleton organization and cellular physiology, with a focus on dysregulation of the gamma-tubulin ring complex (gamma-TuRC) proteins. Recent research on the high-resolution structure of gamma-TuRC provides new insights into the mechanism of microtubule nucleation, potentially offering new drug targets for cancer chemotherapy.
Article
Cell Biology
Anastasiya Klebanovych, Stanislav Vinopal, Eduarda Draberova, Vladimira Sladkova, Tetyana Sulimenko, Vadym Sulimenko, Vera Vosecka, Libor Macurek, Agustin Legido, Pavel Draber
Summary: ER distribution relies on microtubules, and disruption of ER homeostasis activates the unfolded protein response, leading to ER remodeling. UFL1 and C53 interact with gamma-tubulin ring complex proteins and regulate microtubule nucleation in response to ER stress. These findings reveal a novel mechanism for alleviating ER stress through stimulation of centrosomal microtubule nucleation.
Review
Cell Biology
Vadym Sulimenko, Eduarda Draberova, Pavel Draber
Summary: This article reviews the role of gamma-tubulin in microtubule nucleation and its potential functions. Recent studies have provided insights into the structure, activation, and centrosomal recruitment of the gamma-tubulin ring complex (gamma-TuRC), shedding light on the molecular mechanism of microtubule nucleation. Furthermore, gamma-tubulin may also have specific nuclear functions and be involved in cross-talk between microtubules and microfilaments at the centrosome.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Anatomy & Morphology
Veronika Olejnickova, Peter Uriel Hamor, Jiri Janacek, Martin Bartos, Eva Zabrodska, Barbora Sankova, Alena Kvasilova, Hana Kolesova, David Sedmera
Summary: This study reveals the essential role of trabeculae in intraventricular impulse spreading and conduction patterning in the early endothermic heart. Lack of trabeculae leads to a failure in conduction parameters differentiation and impacts the cardiac pumping function.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Ecology
Martina Gregorovicova, Martin Bartos, Bjarke Jensen, Jiri Janacek, Bryan Minne, Jiri Moravec, David Sedmera
Summary: This study focused on the group Anguimorpha and found a correlation between heart morphology, ecological niche, and phylogenetic position.
ECOLOGY AND EVOLUTION
(2022)
Article
Neurosciences
Stanislav Vinopal, Sebastian Dupraz, Eissa Alfadil, Thorben Pietralla, Shweta Bendre, Michael Stiess, Sven Falk, German Camargo Ortega, Nicola Maghelli, Iva M. Tolic, Jiri Smejkal, Magdalena Goetz, Frank Bradke
Summary: Cortical projection neurons polarize and form an axon while migrating radially. The centrosome plays a crucial role in distinguishing the processes of radial migration and axon formation. Dysregulation of centrosomal microtubule nucleation affects radial migration but not axon formation in rodents. This finding provides insight into neuronal migratory defects in human cortical dysgeneses caused by mutations in g-tubulin without majorly affecting axonal tracts.
Article
Multidisciplinary Sciences
Kamila Novotna Floriancicova, Athanasios Baltzis, Jiri Smejkal, Michaela Czernekova, Lukasz Kaczmarek, Jan Maly, Cedric Notredame, Stanislav Vinopal
Summary: Tardigrades, also known as water bears, are able to survive extreme environmental conditions through reversible morphological changes and cryptobiosis. In this study, the molecular composition of tardigrade tubulins, which are important components of the microtubule cytoskeleton, was analyzed and characterized. The results support the phylogenetic placement of tardigrades within the Panarthropoda clade.
SCIENTIFIC REPORTS
(2023)
Meeting Abstract
Immunology
David Habart, Barbora Radochova, Martin Capek, Sarah Suergiu, Zuzana Berkova, Eva Fabryova, Klara Zacharovova, Jiri Janacek, Frantisek Saudek
Article
Multidisciplinary Sciences
Zuzana Zmudova, Zuzana Sanderova, Michaela Liegertova, Stanislav Vinopal, Regina Herma, Ludek Susicky, Monika Mullerova, Tomas Strasak, Jan Maly
Summary: This study evaluated the biodistribution and toxicity of a novel methoxyphenyl phosphonium carbosilane dendrimer in cancer cell cultures and zebrafish embryos. The dendrimer showed high cytotoxicity in cancer cells and distributed within the vascular and gastrointestinal systems in vivo.
SCIENTIFIC REPORTS
(2023)
Article
Biology
Michaela Nejedla, Anastasiya Klebanovych, Vadym Sulimenko, Tetyana Sulimenko, Eduarda Draberova, Pavel Draber, Roger Karlsson
Summary: Profilin 1 is a crucial actin regulator that interacts with microtubules and influences microtubule growth, thus facilitating the coordination of actin and microtubule organization.
LIFE SCIENCE ALLIANCE
(2021)