Article
Fisheries
Yaojia Zhou, Ping Ouyang, Yu Tao, Lizi Yin, Kaiyu Wang, Yi Geng, Weiming Lai, Defang Chen, Hongrui Guo, Jing Fang, Zhengli Chen, Li Tang, Xiaoli Huang
Summary: This study revealed the regulatory role of CyHV-3 vTNFRs in host immune response and cell apoptosis, indicating that vTNFRs participate in virus invasion by up-regulating the expression levels of certain immune-related factors and inducing cell apoptosis.
AQUACULTURE REPORTS
(2021)
Article
Engineering, Biomedical
Hao Yang, Heng Li, Fen Yang, Ze Tao, Qiuxiao Shi, Tianshan She, Yanru Feng, Zhao Li, Jie Chen, Yi Zhong, Tao Su, Wengjuan Zeng, Yong Zhang, Shisheng Wang, Lan Li, Tingting Long, Dan Long, Jingqiu Cheng, Hong Zhu, Xiaofeng Lu
Summary: Increasing the valency of death receptor agonist by promoting higher-order receptor clustering is an efficient way to induce tumor cell apoptosis. However, currently available strategies to improve the clustering ability of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) often result in large proteins with poor tumor penetration. In this study, we demonstrate that covalent protein ligation using small molecular superglues can assemble higher-order TRAIL variants, leading to significantly increased apoptosis induction in vitro and in vivo.
Article
Neurosciences
Peipei Guan, Di Zhu, Pu Wang
Summary: This study demonstrates that meloxicam inhibits neuronal apoptosis by deactivating TNFRSF25, leading to the improvement of memory loss in Alzheimer's disease mice.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Endocrinology & Metabolism
Maki Murakoshi, Tomohito Gohda, Hiroko Sakuma, Terumi Shibata, Eri Adachi, Chiaki Kishida, Saki Ichikawa, Takeo Koshida, Nozomu Kamei, Yusuke Suzuki
Summary: High levels of PGRN and TNFR1 are associated with poor renal prognosis in patients with type 2 diabetes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Daniel J. Lightwood, Rebecca J. Munro, John Porter, David McMillan, Bruce Carrington, Alison Turner, Anthony Scott-Tucker, Elizabeth S. Hickford, Antje Schmidt, David Fox, Alison Maloney, Tom Ceska, Tim Bourne, James O'Connell, Alastair D. G. Lawson
Summary: TNF can be inhibited by small molecules that stabilize the TNF trimer in an asymmetric conformation. The authors also developed a monoclonal antibody that selectively binds this inactive form of TNF, enabling both target engagement assessment and structural characterization of TNF binding to TNF receptor 1.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
K. M. A. Zinnah, Sang-Youel Park
Summary: The study demonstrated the mechanism behind the synergistic anticancer effect of amitriptyline and TRAIL, showing that amitriptyline increases TRAIL-induced apoptosis by upregulating death receptors DR4 and DR5. Inhibition of autophagy by amitriptyline was also shown to enhance DR4 and DR5 expression.
Review
Oncology
Hojjat Alizadeh Zeinabad, Eva Szegezdi
Summary: TRAIL, as a promising anticancer drug with low toxicity, has not been successfully translated into a therapeutic molecule due to its short in vivo half-life and tumor cells' resistance. Nanotechnology shows potential to overcome these limitations and offers better solutions.
Article
Chemistry, Multidisciplinary
Hyeonwoo Je, Gi-Hoon Nam, Gi Beom Kim, Wonjun Kim, Soo Rin Kim, In-San Kim, Eun Jung Lee
Summary: TRAIL shows promising anti-tumor activity, but faces challenges such as resistance and delivery issues. A nanocage has been developed to efficiently deliver TRAIL and a re-sensitizing drug (DOX) to overcome TRAIL-resistant tumors, demonstrating potential as an effective antitumor agent.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
Tianshan She, Fen Yang, Shiyuan Chen, Hao Yang, Ze Tao, Huimin Xing, Jie Chen, Huansheng Chang, Hongyu Lu, Tao Su, Youmei Jin, Yi Zhong, Jingqiu Cheng, Hong Zhu, Xiaofeng Lu
Summary: The clinical application of TRAIL is limited by its inefficient induction of apoptosis in tumor cells. Superglue-mediated hyperoligomerization of TRAIL can increase its valency and improve its efficacy. In this study, minimal superglue peptide pairs were fused to the TRAIL promoter to create superglue-fusion TRAIL variants. These variants showed high expression and trimerization similar to native TRAIL. With the help of Snoopligase or SpyStapler, these variants could be crosslinked into hexavalent TRAIL variants. The hexavalent SnHexaTR variant produced by Snoopligase showed the highest yield and exhibited 10-40 times greater cytotoxicity than native TRAIL in tumor cells. It also had a longer half-life and greater tumor uptake, leading to the eradication of tumor xenografts. Hexavalent SnHexaTR, as a novel anticancer agent candidate, has great potential for clinical application in cancer therapy.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Oncology
Shihai Liu, Jing Qiu, Guifang He, Weitai He, Changchang Liu, Duo Cai, Huazheng Pan
Summary: The interaction between TRAIL and IER3 can induce apoptotic death of HCC cells and inhibit their proliferation and migration by inhibiting Wnt/beta-catenin signaling. This TRAIL/IER3/beta-catenin axis may serve as a viable therapeutic target for HCC patients.
CANCER CELL INTERNATIONAL
(2021)
Article
Medicine, Research & Experimental
Wen-juan Jiang, Chuan-ting Xu, Chang-lin Du, Jia-hui Dong, Song-bing Xu, Bing-feng Hu, Rui Feng, Dan-dan Zang, Xiao-ming Meng, Cheng Huang, Jun Li, Tao-tao Ma
Summary: This study revealed a novel cell communication mechanism between tubular epithelial cells and macrophages in diabetic nephropathy. The communication is mediated by extracellular vesicles enriched with LRG1 and TRAIL, leading to worsened kidney damage and activation of inflammation.
Article
Pharmacology & Pharmacy
Yu Ren, Xue Wang, Shuaishuai Huang, Yangkai Xu, Guobin Weng, Rui Yu
Summary: In this study, we found that alternol sensitized renal carcinoma cells to TRAIL-induced apoptosis by inhibiting antiapoptotic proteins, upregulating DR5 levels, ROS generation, and the CHOP pathway, thus enhancing TRAIL-mediated apoptosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Bingyu Sun, Yongqiang Liu, Danhua He, Jinke Li, Jiawei Wang, Wulin Wen, Ming Hong
Summary: Current chemotherapy agents have severe cytotoxicity to normal cells, resulting in decreased quality of life. Traditional Chinese medicine ingredients have shown potential to sensitize tumor cells to TRAIL-induced apoptosis, offering new insights for cancer treatment.
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2021)
Article
Medicine, Research & Experimental
Nihal Karakas, Daniel Stuckey, Esther Revai-Lechtich, Khalid Shah
Summary: The study revealed that using IL13-PE or ENb-PE could upregulate TRAIL death receptors, suppress the expression of anti-apoptotic proteins, and sensitize highly resistant GBM cells to TRAIL-mediated apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Oncology
Min Huang, Cheng Yi, Xian-Zhou Huang, Juan Yan, Li-Jia Wei, Wei-Ju Tang, Shou-Chun Chen, Ying Huang
Summary: TRAIL-Mu3 exhibits stronger antitumor effects on pancreatic cancer cells compared with TRAIL by enhancing the apoptotic signaling pathway.
Article
Biochemistry & Molecular Biology
Sven O. Dahms, Kornelia Hardes, Torsten Steinmetzer, Manuel E. Than
Article
Biochemistry & Molecular Biology
Elfriede Dall, Julia C. Hollerweger, Sven O. Dahms, Haissi Cui, Katharina Haeussermann, Hans Brandstetter
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Hematology
Sven O. Dahms, Fatih Demir, Pitter F. Huesgen, Karina Thorn, Hans Brandstetter
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2019)
Article
Chemistry, Medicinal
Thuy Van Lam Van, Teodora Ivanova, Kornelia Hardes, Miriam Ruth Heindl, Rory E. Morty, Eva Boettcher-Friebertshaeuser, Iris Lindberg, Manuel E. Than, Sven O. Dahms, Torsten Steinmetzer
Article
Chemistry, Analytical
Wai Tuck Soh, Fatih Demir, Elfriede Dall, Andreas Perrar, Sven O. Dahms, Maithreyan Kuppusamy, Hans Brandstetter, Pitter F. Huesgen
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Elfriede Dall, Florian B. Zauner, Wai Tuck Soh, Fatih Demir, Sven O. Dahms, Chiara Cabrele, Pitter F. Huesgen, Hans Brandstetter
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Chemistry, Medicinal
Thuy Van Lam van, Miriam Ruth Heindl, Christine Schlutt, Eva Boettcher-Friebertshaeuser, Ralf Bartenschlager, Gerhard Klebe, Hans Brandstetter, Sven O. Dahms, Torsten Steinmetzer
Summary: Furin activates viral glycoproteins, and inhibiting it can prevent virus replication. New inhibitors targeting furin have been developed, showing potent antiviral activity in cell culture.
ACS MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Sven O. Dahms, Tanja Haider, Gerhard Klebe, Torsten Steinmetzer, Hans Brandstetter
Summary: The pro-protein convertase furin is an important enzyme that activates surface proteins of many viruses, including SARS-CoV-2. Inhibitors targeting furin can effectively suppress viral replication, showing broad application potential. Targeting furin in its OFF state can serve as a valuable strategy for structure-based development of PC-selective small-molecule inhibitors.
ACS CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Physical
Elfriede Dall, Vesna Stanojlovic, Fatih Demir, Peter Briza, Sven O. Dahms, Pitter F. Huesgen, Chiara Cabrele, Hans Brandstetter
Summary: Legumain, a dual protease-ligase, plays a key role in the generation of antigenic peptides and can catalyze ligation and cyclization of linear peptides at near-neutral pH conditions. The enzyme's activity can be significantly enhanced through conformational stabilization and engineering prime substrate recognition sites. This research provides a basis for engineering legumain proteases and ligases, with potential applications in biotechnology and drug development.
Article
Biochemistry & Molecular Biology
Sven O. Dahms, Gisela Schnapp, Martin Winter, Frank H. Buttner, Marco Schlepuetz, Christian Gnamm, Alexander Pautsch, Hans Brandstetter
Summary: (3,5-dichlorophenyl)pyridine-derived furin inhibitors have been reported to have high cellular potency and antiviral activity against SARS-CoV-2. In this study, the binding mechanism of this inhibitor class was characterized using structural, biophysical, and biochemical methods. The discovered furin conformation offers new opportunities for structure-based drug discovery.
ACS CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Vartika Mishra, Diane B. Re, Virginia Le Verche, Mariano J. Alvarez, Alessandro Vasciaveo, Arnaud Jacquier, Paschalis-Tomas Doulias, Todd M. Greco, Monica Nizzardo, Dimitra Papadimitriou, Tetsuya Nagata, Paola Rinchetti, Eduardo J. Perez-Torres, Kristin A. Politi, Burcin Ikiz, Kevin Clare, Manuel E. Than, Stefania Corti, Harry Ischiropoulos, Francesco Lotti, Andrea Califano, Serge Przedborski
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Medicinal
Teodora Ivanova, Kornelia Hardes, Stephanie Kallis, Sven O. Dahms, Manuel E. Than, Sebastian Kuenzel, Eva Boettcher-Friebertshaeuser, Iris Lindberg, Guan-Sheng Jiao, Ralf Bartenschlager, Torsten Steinmetzer
Correction
Biochemistry & Molecular Biology
Sven O. Dahms, Guan-Sheng Jiao, Manuel E. Than
ACS CHEMICAL BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Ankita Chadda, Alexander G. Kozlov, Binh Nguyen, Timothy M. Lohman, Eric A. Galburt
Summary: In this study, it was found that the DNA damage response in Mycobacterium tuberculosis differs from well-studied model bacteria. The DNA repair helicase UvrD1 in Mtb is activated through a redox-dependent process and is closely associated with the homo-dimeric Ku protein. Additionally, Ku protein is shown to stimulate the helicase activity of UvrD1.
JOURNAL OF MOLECULAR BIOLOGY
(2024)