Review
Nutrition & Dietetics
Jeyakumar Balakrishnan, Suganya Kannan, Ambujam Govindasamy
Summary: Docosahexaenoic acid (DHA) is crucial for brain function and cognitive development, and deficiency can lead to neurodegenerative disorders. Recent studies suggest that lysophosphatidylcholine (lysoPC) form of DHA is better absorbed by the brain, and supplementation with this form can decrease the risk of such disorders.
NUTRITION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Elisabeth M. Haberl, Thomas S. Weiss, Georg Peschel, Kilian Weigand, Nikolai Koehler, Josch K. Pauling, Juergen J. Wenzel, Marcus Hoering, Sabrina Krautbauer, Gerhard Liebisch, Christa Buechler
Summary: The study revealed differences in lipid metabolism between liver cancer tissues and non-tumor liver tissues, as well as in the impact of HBV and HCV infections on hepatic lipid concentrations. The tumor suppressor protein p53 showed a negative correlation with free cholesterol in tumors, indicating its role in cholesterol metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Spectroscopy
Mikael Fabritius, Baoru Yang
Summary: Analysis of triacylglycerol (TG) and phospholipid sn-positional isomers can be done through direct separation or quantification of regioisomer ratios using mass spectrometry. Direct chromatography separation is being replaced by mass spectrometry due to limitations. However, challenges arise from the large number of isobaric and isomeric lipid species in natural samples and the lack of available regiopure standards. Optimization algorithms and fragmentation models are useful for analysis of TG regioisomers.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Biochemical Research Methods
Zari Saadati Nezhad, Juan Pablo Salazar, Rachel S. Pryce, Lisa M. Munter, Pierre Chaurand
Summary: Cholesterol is crucial for animal life and is dysregulated in many diseases. A new method using silver-assisted laser desorption ionization mass spectrometry imaging (AgLDI MSI) has been developed for absolute quantification of cholesterol in brain samples, providing better insight into the role of cholesterol in disease onset and progression.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Leonidas Mavroudakis, Kyle D. Duncan, Ingela Lanekoff
Summary: A new method incorporating crown ether molecules in the solvent for nano-DESI MSI allows for quantitative imaging of alkali metal ions and endogenous lipids/metabolites. Applied to an ischemic stroke model, this method revealed dynamic tissue sodium and potassium concentrations, along with changes in lipids, neurotransmitters, and amino acids.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Changfeng Hu, Jingyi Song, Wumeng Jin, Wenqiang Wang, Hongjin Bai, Cuiyun Wu, Lirong Shen
Summary: Walnut oil, rich in polyunsaturated fatty acids, has many health benefits. The synthesis and accumulation of walnut oil in the embryo development process may be influenced by a special mechanism for triacylglycerol biosynthesis. Shotgun lipidomics analysis revealed that triacylglycerol synthesis in walnut kernels occurred before 84 days after flowering and was enhanced between 84 and 98 days. Acyl editing through phosphatidylcholine played a role in the flux of fatty acids for triacylglycerol synthesis. Therefore, triacylglycerol biosynthesis in walnut kernels is directly linked to lipid metabolism.
Article
Biochemical Research Methods
Hyun Jun Jang, Minh Uyen Thi Le, Jeong Hyang Park, Chang Geon Chung, Jin Gyeong Shon, Ga Seul Lee, Jeong Hee Moon, Sung Bae Lee, Joon Sig Choi, Tae Geol Lee, Sohee Yoon
Summary: Changes in phospholipid distribution in ALS-induced Drosophila brain tissue can be investigated through MALDI-MSI, revealing early disease indicators of ALS; Increasing culture time leads to differences in lipid profiles according to phospholipids.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Multidisciplinary Sciences
Gianluca Sighinolfi, Samantha Clark, Landry Blanc, Daniela Cota, Boutayna Rhourri-Frih
Summary: Studies have shown that obesity is closely related to changes in brain structure and function. Through experiments on mice, it was found that a high-fat diet can rapidly alter the brain lipidome, with some changes persisting even after weight loss.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yang Bai, Xiaofang Zhu, Xinya Guo, Wenhua Zhang, Guozheng Zhang, Huatao Chen, Qun Zhang
Summary: The study isolated and characterized two AAPTs, GmAAPT1 and GmAAPT2, from soybeans, which showed strong similarity in amino acid contents and expression patterns and were found to localize to the endoplasmic reticulum and Golgi apparatus. In vitro enzymatic analyses revealed that GmAAPT1 and GmAAPT2 contributed to PC and PE synthesis, playing a role in regulating seed TAG and polyunsaturated fatty acid levels. Overexpression of GmAAPT1 and GmAAPT2 in Arabidopsis resulted in decreased seed TAG and polyunsaturated fatty acids levels, leading to reduced seed germination under freezing stress. This suggests that GmAAPTs mediate TAG metabolism and negatively regulate seed freezing tolerance.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Multidisciplinary Sciences
Huizhen Ma, Xiaoning Zhang, Ling Liu, You Huang, Si Sun, Ke Chen, Qi Xin, Pengfei Liu, Yuxing Yan, Yili Wang, Yuan Li, Haile Liu, Ruoli Zhao, Kexin Tan, Xinzhu Chen, Xun Yuan, Yonghui Li, Ying Liu, Haitao Dai, Changlong Liu, Hao Wang, Xiao-Dong Zhang
Summary: To address the issue of low bioactivity in most near-infrared-II (NIR-II) fluorophores, researchers have developed atomically precise Au-22 clusters with strong NIR-II fluorescence and high catalytic activities. The newly developed Au21Cu1 clusters show significantly higher antioxidant, catalase-like, and superoxide dismutase-like activities than Au-22 clusters, with negligible fluorescence loss. These clusters can be used for three-dimensional imaging of cisplatin-induced renal injury and inhibit oxidative stress and inflammation in a mouse model.
Article
Medicine, Research & Experimental
Sophie Strindberg, Jakob Plum, Charlotte Bagger, Christian Janfelt, Anette Mullertz
Summary: Mapping the distribution of a prodrug (fenofibrate) through the rat gastrointestinal tract using MALDI-MSI revealed hydrolysis of fenofibrate to the active drug in the stomach. The study also found the presence of taurocholate and phospholipids in the lumen of the small intestine.
MOLECULAR PHARMACEUTICS
(2021)
Article
Food Science & Technology
Xin Wang, Yuning Chen, Yue Liu, Lei Ouyang, Ruonan Yao, Zhihui Wang, Yanping Kang, Liying Yan, Dongxin Huai, Huifang Jiang, Yong Lei, Boshou Liao
Summary: The spatial distribution of lipids and other key components in three peanut cultivars was investigated using the MALDI-MSI technique. The study revealed a non-homogeneous spatial distribution of metabolites, which is related to the different functions of particular tissue regions. These findings have implications for the development of oil crops with higher oil yields and improvement of food processing.
Article
Biochemistry & Molecular Biology
Jan Hahn, Manuela Moritz, Hannah Voss, Penelope Pelczar, Samuel Huber, Hartmut Schlueter
Summary: Recent studies have shown that nanosecond infrared lasers are effective for tissue sampling and homogenization, suitable for mass spectrometric proteomics research. Liquid chromatography tandem mass spectrometry analysis revealed significant abundance differences in proteins between two tissue types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Laura M. Cole, Joshua Handley, Emmanuelle Claude, Catherine J. Duckett, Hardeep S. Mudhar, Karen Sisley, Malcolm R. Clench
Summary: The MALDI-MSI technique was used to image lipid and metabolite distribution in FFPE whole eye sections containing UM, highlighting LPC lipid distribution in tumor regions. LA-ICP-MS imaging on UM sections revealed increased copper in the tumor periphery and intratumoral zinc in tissue from patients with poor prognosis. These preliminary findings suggest that multi-modal MSI may offer insights into the role of trace metals in cancer metastasis.
Article
Biochemical Research Methods
Assim A. Alfadda, Hicham Benabdelkamel, Amany A. Fathaddin, Ibrahim O. Alanazi, Nidia Lauzon, Pierre Chaurand, Afshan Masood
Summary: Obesity is associated with thyroid dysfunction and abnormal protein distribution in thyroid tissue. A study analyzed protein changes between lean and obese patients using MALDI imaging mass spectrometry technique. The study found differences in protein levels in thyroid tissue between lean and obese patients, suggesting a potential link between obesity and thyroid disorders.
JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Karin A. Zemski Berry, Robert M. Barkley, Joseph J. Berry, Joseph A. Hankin, Emmy Hoyes, Jeffery M. Browns, Robert C. Murphy
ANALYTICAL CHEMISTRY
(2017)
Article
Immunology
Caroline Turcotte, Simona Zarini, Stephanie Jean, Cyril Martin, Robert C. Murphy, David Marsolais, Michel Laviolette, Marie-Renee Blanchet, Nicolas Flamand
JOURNAL OF IMMUNOLOGY
(2017)
Article
Dermatology
Kathleen A. Harrison, Eric Romer, Jonathan Weyerbacher, Jesus A. Ocana, Ravi P. Sahu, Robert C. Murphy, Lisa E. Kelly, Townsend A. Smith, Christine M. Rapp, Christina Borchers, David R. Cool, Gengxin Li, Richard Simman, Jeffrey B. Travers
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Toshiaki Okuno, Miguel A. Gijon, Simona Zarini, Sarah A. Martin, Robert M. Barkley, Christopher A. Johnson, Mai Ohba, Takehiko Yokomizo, Robert C. Murphy
JOURNAL OF LIPID RESEARCH
(2018)
Article
Biochemical Research Methods
James P. Di Giovanni, Robert M. Barkley, David N. M. Jones, Joseph A. Hankin, Robert C. Murphy
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2018)
Review
Chemistry, Analytical
Robert C. Murphy
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Robert C. Murphy, Giancarlo Folco
JOURNAL OF LIPID RESEARCH
(2019)
Review
Biochemistry & Molecular Biology
Valerie B. O'Donnell, Maceler Aldrovandi, Robert C. Murphy, Gerhard Kroenke
Article
Biochemistry & Molecular Biology
Andrei Kornilov, Paul D. Kennedy, Maceler Aldrovandi, Andrew J. A. Watson, Christine Hinz, Bryan Harless, Joseph Colombo, Kirk M. Maxey, Victoria J. Tyrrell, Matthew Simon, Varinder K. Aggarwal, William E. Boeglin, Alan R. Brash, Robert C. Murphy, Valerie B. O'Donnell
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemical Research Methods
Robert C. Murphy
JOURNAL OF MASS SPECTROMETRY
(2020)
Article
Biochemistry & Molecular Biology
Jae-Yeon Choi, Raymond Black, Hee-Jung Lee, James Di Giovanni, Robert C. Murphy, Choukri Ben Mamoun, Dennis R. Voelker
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Cell Biology
Bailey A. Loving, Maoping Tang, Mikaela C. Neal, Sachi Gorkhali, Robert Murphy, Robert H. Eckel, Kimberley D. Bruce
Summary: As individuals age, dysfunctional microglia may contribute to the onset of neurodegenerative diseases through impaired phagocytosis, cholesterol efflux, and enhanced secretion of pro-inflammatory cytokines. Specifically, microglia lacking lipoprotein lipase exhibit a pro-inflammatory lipid profile, increased cholesterol ester content, and reduced cholesterol efflux, leading to excessive accumulation of lipid droplets. Activation of PPAR pathways by agonists can rescue this phenotype, suggesting a potential therapeutic strategy for aging and neurodegenerative diseases.
Article
Immunology
Robert C. Murphy, Sven Hammarstrom, Bengt Samuelsson
JOURNAL OF IMMUNOLOGY
(2018)
Article
Chemistry, Analytical
Robert C. Murphy, Toshiaki Okuno, Christopher A. Johnson, Robert M. Barkleyte
ANALYTICAL CHEMISTRY
(2017)
Meeting Abstract
Biochemistry & Molecular Biology
Miguel A. Gijon, Ann-Charlotte Almstrand, Chris A. Johnson, Robert C. Murphy, Simona Zarini