Subsets of CD4+, CD8+, and CD25hiLymphocytes Are in General Not Influenced by Isolation and Long-Term Cryopreservation

An Integrated Workflow To Assess Technical and Biological Variability of Cell Population Frequencies in Human Peripheral Blood by Flow Cytometry

(2017) Julie G. Burel et al.   JOURNAL OF IMMUNOLOGY

The effect of cellular isolation and cryopreservation on the expression of markers identifying subsets of regulatory T cells

(2016) Weiying Zhang et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

(2015) Christopher P. Corkum et al.   BMC IMMUNOLOGY

The effects of freeze/thawing on the function and phenotype of CD4+ lymphocyte subsets in normal individuals and patients with systemic lupus erythematosus

(2015) Vanessa da Silva Ferreira et al.   CRYOBIOLOGY

Recommendations for the evaluation of specimen stability for flow cytometric testing during drug development

(2015) Lynette Brown et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Stable Phenotype Of B-Cell Subsets Following Cryopreservation and Thawing of Normal Human Lymphocytes Stored in a Tissue Biobank

(2014) Simon Mylius Rasmussen et al.   CYTOMETRY PART B-CLINICAL CYTOMETRY

Temperature fluctuations during deep temperature cryopreservation reduce PBMC recovery, viability and T-cell function

(2013) Anja Germann et al.   CRYOBIOLOGY

Validation of cell-based fluorescence assays: practice guidelines from the ICSH and ICCS - part II - preanalytical issues

(2013) Bruce H. Davis et al.   CYTOMETRY PART B-CLINICAL CYTOMETRY

Challenges in cryopreservation of regulatory T cells (Tregs) for clinical therapeutic applications

(2013) Karolina Golab et al.   INTERNATIONAL IMMUNOPHARMACOLOGY

Expression of IL-7 receptor in human peripheral regulatory T cells

(2012) Maciej Ciebiada et al.   Archives of Medical Science

FLOW CYTOMETRIC LYMPHOCYTE SUBSET ANALYSIS USING MATERIAL FROM FROZEN WHOLE BLOOD

(2012) Iftikhar Alam et al.   JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY

Cryopreservation modulates the detection of regulatory T cell markers

(2011) Sebastian Sattui et al.   CYTOMETRY PART B-CLINICAL CYTOMETRY

Comparison of cryopreservation methods on T-cell responses to islet and control antigens from type 1 diabetic patients and controls

(2011) B. Brooks-Worrell et al.   DIABETES-METABOLISM RESEARCH AND REVIEWS

Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society

(2010) R. Mallone et al.   CLINICAL AND EXPERIMENTAL IMMUNOLOGY

Different Proliferative Potential and Migratory Characteristics of Human CD4+ Regulatory T Cells That Express either CD45RA or CD45RO

(2010) N. J. Booth et al.   JOURNAL OF IMMUNOLOGY

Optimization and Limitations of Use of Cryopreserved Peripheral Blood Mononuclear Cells for Functional and Phenotypic T-Cell Characterization

(2009) A. Weinberg et al.   Clinical and Vaccine Immunology

Functional Delineation and Differentiation Dynamics of Human CD4+ T Cells Expressing the FoxP3 Transcription Factor

(2009) Makoto Miyara et al.   IMMUNITY

How and when to pick up the best signals from markers associated with T-regulatory cells?

(2009) Anna Kivling et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

(2009) Eyad Elkord   JOURNAL OF IMMUNOLOGICAL METHODS

Cryopreservation does not alter the frequency of regulatory T cells in peripheral blood mononuclear cells

(2009) D. Van Hemelen et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Optimal Blood Mononuclear Cell Isolation Procedures for Gamma Interferon Enzyme-Linked Immunospot Testing of Healthy Swedish and Tanzanian Subjects

(2008) C. Nilsson et al.   Clinical and Vaccine Immunology

Phenotype and function of human T lymphocyte subsets: Consensus and issues

(2008) Victor Appay et al.   CYTOMETRY PART A

Phenotypic classification of human CD4+ T cell subsets and their differentiation

(2008) R. Okada et al.   INTERNATIONAL IMMUNOLOGY