Article
Chemistry, Applied
Megan C. Y. Ooi, Xiaojie Zhang, Christopher M. Beaudry, Juyun Lim, Michael H. Penner
Summary: The study focuses on developing economical approaches for the preparation and characterization of prebiotic oligosaccharides with different compositions and degrees of polymerization (DP). Food-grade fructooligosaccharides of DP 3 and 4, galactooligosaccharides of DP 3 and 4, and xylooligosaccharides of DP 2-4 were successfully prepared through chromatographic fractionation. Methods for assessing the purity, identity, total carbohydrate content, moles per unit mass, and DP of the oligosaccharides were also presented.
Article
Chemistry, Applied
Toren S. Andrewson, Laura E. Martin, Juyun Lim, Michael H. Penner
Summary: Flash chromatography with microcrystalline cellulose stationary phases and aqueous ethanol mobile phases have potential for producing food-grade oligosaccharides, but caution must be taken to avoid contamination. This study presents a strategy to mitigate contamination by washing the stationary phase with appropriate aqueous ethanol solutions before oligosaccharide fractionation.
Article
Chemistry, Multidisciplinary
Wanyi Xie, Shixuan He, Shaoxi Fang, Rong Tian, Liyuan Liang, Deqiang Wang
Summary: This study proposes a nanopipette-based method assisted by PEI-oBA to detect neutral polysaccharides with different degrees of polymerization. The combination of PEI-oBA and the target molecule enhances the electrophoretic force and exclusion volume, resulting in nanopore detection with a high signal-to-noise ratio.
Article
Integrative & Complementary Medicine
Hyang-Im Baek, Ki-Chan Ha, Yu Kyung Park, Je Hyeon Lee, Eun Joo Kim, Hye-Jeong Ko, Jong Cheon Joo
Summary: This study found that the use of NAOs significantly reduces visceral fat area and the ratio of visceral-subcutaneous fat area. Compared to the placebo group, the NAOs group showed smaller increases in weight and BMI. Additionally, the NAOs group had an increase in high-density lipoprotein-cholesterol levels. No clinical changes were observed for any safety biomarkers.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2023)
Article
Chemistry, Analytical
Yan Li, Xiaofan Tang, Yinhai Li, Weilong Zhao, Shengwei Guo, Chunmiao Bo
Summary: In this study, a developed chromatographic stationary phase combined the high selectivity of mixed-mode retention with a temperature-responsive property to enhance separation efficiency. The results showed that the stationary phase could achieve multiple interactions towards different types of analytes on one column, and the separation selectivity could be adjusted by changing the column temperature.
ANALYTICAL METHODS
(2023)
Article
Engineering, Chemical
I Mueller, A. Seidel-Morgenstern, C. Hamel
Summary: A commercial galacto-oligosaccharide (GOS) syrup with prebiotic properties was purified using simulated moving bed (SMB) chromatography to separate undesired side products induced by synthesis reaction mechanism. The chosen system allows pseudo binary separation of GOS syrup mixture, producing highly purified products with purities up to >99%, indicating significant potential for scale-up.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Chemistry, Analytical
Yi Zheng, Jingyu Yan, Cuiyan Cao, Yanfang Liu, Dongping Yu, Xinmiao Liang
Summary: Polysaccharides are widely present in nature, ranging from monocytic microorganisms to higher animals, and have been found to have various biological activities in recent decades. However, natural polysaccharides have the characteristics of large molecular weight, diverse composition, and complex structure, making their purification and structural analysis challenging in research. Chromatography, as a powerful separation technique, plays an indispensable role in the separation and structural analysis of natural polysaccharides, especially in the purification of polysaccharides, separation of hydrolysates, and analysis of monosaccharide composition. This review summarizes the separation mechanisms and applications of different chromatographic methods in polysaccharide studies, and discusses the advantages and drawbacks of various chromatography methods.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Chia Yen Liew, Hong-Sheng Luo, Ting-Yi Yang, An-Ti Hung, Bryan John Abel Magoling, Charles Pin-Kuang Lai, Chi-Kung Ni
Summary: This study re-examines high mannose N-glycans in multicellular eukaryotes using a new mass spectrometry method, and identifies many previously unreported isomers. A database is constructed for rapid identification of high mannose N-glycan isomers.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jinxi Cheng, Xiaoyan Hou, Qiang Cui, Guanghui Shen, Shanshan Li, Qingying Luo, Man Zhou, Hong Chen, Zhiqing Zhang
Summary: This study successfully isolated and purified hydroxyl-alpha-sanshool, the main alkylamide produced by Z. armatum. The method used was simple, rapid, economical, and effective, providing a new approach for the separation of high-purity hydroxyl-alpha-sanshool.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Applied
Shuang Lu, Kai Na, Jiani Wei, Li Zhang, Xiaohua Guo
Summary: Alginate oligosaccharides (AOS), degradation products of brown algae, have diverse biological activities including anti-inflammatory, antimicrobial, antioxidant, and antitumor properties. Enzymatic hydrolysis of alginate produces different types of AOS, with the hybrid form HAOS being the most extensively studied. Systematic studies on the structures and biological activities of AOS are necessary for precise application.
CARBOHYDRATE POLYMERS
(2022)
Article
Food Science & Technology
Silke L. Pittrof, Larissa Kaufhold, Anja Fischer, Daniel Wefers
Summary: This study comparatively investigated the product patterns obtained from the incubation of linear as well as O3- and O4-branched dextrans with different dextranases, aiming to understand the differences in their hydrolysis products.
Article
Biochemical Research Methods
Vanessa Wong, Laura Pascual Fernandez, Praveen Kallamvalli Illam Sankaran, Alexander Holt, Rahul Mishra, Vaishali Sinha, Viv Lindo
Summary: This study presents a three-step approach to detect, confirm, and characterize partially reduced fragment species in an IgG1 monoclonal antibody. Microchip capillary electrophoresis and high-performance size exclusion chromatography were used for fast screening, while capillary electrophoresis-sodium dodecyl sulphate and reverse phase high-performance liquid chromatography coupled with mass spectrometry were employed for confirmation and characterization. The study provides a systematic strategy for process development and investigation of anomalous events in manufacturing, and compares modern and conventional analytical techniques for fragment analysis.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Analytical
Rakesh Chandrakant Prabhu, Arthanareeswari Maruthapillai, Gopal Chandru Senadi
Summary: The article describes the development of a fast, selective, and precise analytical method using a statistically driven stage-wise AQbD approach with proven orthogonality. Challenging the method with orthogonality proved its robustness, in line with ICH guidelines for validation.
ANALYTICAL CHEMISTRY
(2021)
Article
Polymer Science
Elena Trofimchuk, Valeria Ostrikova, Olga Ivanova, Marina Moskvina, Anna Plutalova, Tatyana Grokhovskaya, Anna Shchelushkina, Alexander Efimov, Elena Chernikova, Shenghua Zhang, Vladimir Mironov
Summary: The degradation of polylactide (PLA) films of different structures under conditions of controlled composting has been studied. The researchers demonstrated that PLA can undergo degradation within one month in a substrate that simulates standard industrial composting. Regardless of the initial structure of the samples, the number-average molecular weight (Mn) decreased to 4 kDa while the degree of crystallinity increased to about 70% after 21 days of composting. Addition of an inoculant to the standard substrate resulted in accelerated degradation of the PLA samples for one week due to abiotic hydrolysis. These findings confirm that industrial composting can potentially address the issue of PLA plastic disposal.
Article
Chemistry, Physical
Chen Ma, Yumin Liu, Lili Zhang, Liu Qian, Yiming Zhao, Ying Tian, Qianru Wu, Dong Li, Nan Zhao, Xueting Zhang, Liantao Xin, Huaping Liu, Pengxiang Hou, Chang Liu, Maoshuai He, Jin Zhang
Summary: In this study, a porous magnesia support rhenium catalyst was proposed for bulk synthesis of SWNTs, and it was found that the produced SWNTs are dominant in (n,n-1) and (n,n-2) species. Furthermore, SWNTs with different diameter ranges were separated using a multicolumn chromatography method.
Article
Biochemistry & Molecular Biology
Bin Wei, Sijia Wang, Yakun Wang, Songze Ke, Weihua Jin, Jianwei Chen, Huawei Zhang, Jiadong Sun, Susanne M. Henning, Jian Wang, Hong Wang
Summary: The study found that a high-fat diet affects the body weight and fasting blood glucose of high-fat DIO mice, as well as altering the host-microbial co-metabolism and gut microbiota composition. In contrast, in mice resistant to high-fat DIO, the high-fat diet did not increase body weight but did significantly increase fasting blood glucose.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Article
Chemistry, Applied
Qi-Wu Zhong, Tao-Shun Zhou, Wen-Hui Qiu, Ya-Kun Wang, Qiao-Li Xu, Song-Ze Ke, Si-Jia Wang, Wei-Hua Jin, Jian-Wei Chen, Hua-Wei Zhang, Bin Wei, Hong Wang
Summary: The brown seaweed Undaria pinnatifida polysaccharides have shown potential hypoglycemic effects by reducing postprandial and induced hyperglycemia through improved glucose uptake and insulin resistance. These polysaccharides could be a promising agent for diabetes treatment.
Article
Pharmacology & Pharmacy
Yu Yi, Jianming Hu, Shenwei Ding, Jianfeng Mei, Xudong Wang, Yanlu Zhang, Jianshu Chen, Guoqing Ying
Summary: A feasible strategy for directing protein immobilization on silica magnetic beads for ligand fishing based on SpyTag/SpyCatcher (ST/SC)-mediated anchoring is presented. This method allows for the preparation of diverse protein-functionalized SMBs for drug screening and target finding.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Jianfeng Mei, Xia Wu, Sujing Zheng, Xiang Chen, Zhuliang Huang, Yichun Wu
Summary: In this study, the beta-glucosidase gene bglS from Streptomyces sp. RW-2 was successfully cloned and expressed, enabling efficient biotransformation of CuBg to CuB with a conversion rate of 87.6%, leading to an increase in CuB concentration from 13.6 to 20.2 g/L in C. melo pedicel extracts. This research provides a high-efficiency technology for producing CuB from its glycoside through biotransformation.
Article
Biotechnology & Applied Microbiology
Jianfeng Mei, Xia Wu, Sujing Zheng, Yu Yi, Xudong Wang, Guoqing Ying
Summary: Bilirubin, a valuable Chinese medicine, is currently produced from pig bile, but due to raw material shortage, its price has increased. This study explores the use of biotransformation to produce bilirubin using recombinant E. coli expressing biliverdin reductase. With optimized conditions, the conversion yield reached 72.3%. This provides a potential alternative process for bilirubin production.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Oncology
Hong Shen, Qiqi Zhao, Bilian Chen, Chao Li, Jue Li, Han Sun, Yu Chen, Wanqin Chen, Yu Yi, Jianfeng Mei, Yanlu Zhang, Guoqing Ying
Summary: This study aimed to establish an enzyme-linked immunosorbent assay (ELISA)-based method to detect nicarbazin drug residues with high sensitivity and specificity, and wide applicability. The research reports on the detection methods of nicarbazin residues are mainly based on large-scale instruments such as high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry-mass spectrometry (LC/MS/MS) and so on. However, in the practical monitoring and detection application, the rapid, sensitive, efficient and accurate detection of nicarbazin residues is becoming more and more urgent. This method, which uses a specific antibody against the nicarbazin metabolic product DNC, enables rapid quantitative detection. Our new ELISA-based method should facilitate the development of assays to monitor and detect agricultural and veterinary drug residues.
ANNALS OF TRANSLATIONAL MEDICINE
(2022)
Article
Nutrition & Dietetics
Hong Shen, Chao Li, Han Sun, Wanqin Chen, Bilian Chen, Yu Yi, Jianfeng Mei, Yanlu Zhang, Guoqing Ying
Summary: An anti-diclazuril monoclonal antibody (mAb) with high sensitivity and specificity was developed for detecting diclazuril residues. The method showed excellent performance in terms of detection limit, sensitivity, and recovery rate.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Haohua Liu, Yue Cai, Yuteng Chu, Xiaojie Yu, Fuhang Song, Hong Wang, Huawei Zhang, Xuanrong Sun
Summary: Chrysomycin A, derived from marine microorganisms, has shown significant in vitro inhibitory effect against methicillin-resistant Staphylococcus aureus (MRSA). To overcome its poor water solubility, a chrysomycin A cream was developed for the treatment of skin infections. The cream had a smooth and lustrous surface, met the particle size requirements, and exhibited long-term stability. In vitro and in vivo studies demonstrated that the cream had similar anti-MRSA activity to the commercially available mupirocin, suggesting its potential as an effective topical delivery system for skin infection treatment.
Article
Chemistry, Physical
Jianfeng Mei, Xiang Chen, Pingya Wang, Yichun Wu, Yu Yi, Guoqing Ying
Summary: This study presents an alternative method for taxifolin production by extracting astilbin from plants, which is more readily available.
Article
Biochemical Research Methods
Yu Yi, Kefan Shi, Shenwei Ding, Jianming Hu, Cheng Zhang, Jianfeng Mei, Guoqing Ying
Summary: In this study, a facile and efficient protein affinity-ligand oriented-immobilization strategy based on SpyTag/SpyCatcher chemistry was developed for bioactive compound screening. The feasibility of this method was verified using GFP and PqsA as model proteins. The results showed that this strategy can immobilize protein ligands in one-step and screen compounds that interact specifically with the ligands.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Guishun Bai, Yang Yang, Xingyue Wang, Jiamin Wu, Hong Wang, Xinyi Ye, Xiaoze Bao
Summary: A DBU-promoted cascade reaction for the synthesis of polysubstituted arenes was reported, featuring mild reaction conditions, wide substrate scope, and high yield. The enantioselective version of this reaction was explored to produce chiral biaryl atropisomers.
Article
Biochemical Research Methods
Yu Yi, Mengyuan Cui, Shupeng Song, Cheng Zhang, Jianfeng Mei, Guoqing Ying
Summary: In this study, a fusion protein ZZ-Mfp-5 was constructed and expressed, which successfully achieved directional immobilization of antibodies and capture of antigens in solid-phase immunoassays. This strategy significantly improved the stability, sensitivity, and reproducibility of the detection process.
JOURNAL OF IMMUNOLOGICAL METHODS
(2023)
Review
Chemistry, Medicinal
Wei Wang, Liting Gu, Jiahao Wang, Xubin Hu, Bin Wei, Huawei Zhang, Hong Wang, Jianwei Chen
Summary: In the post-antibiotic era, the rapid development of antibiotic resistance and the shortage of available antibiotics have created a new health-care crisis. Small-molecule antimicrobial peptides, with their rich structural diversity and innovative antimicrobial mechanisms, have emerged as promising candidates for next-generation antibiotics. In this article, we present a collection of small-molecule antimicrobial peptides derived from marine microorganisms over the past fifteen years, categorizing them into three classes based on their structural features. We provide information on their sources, structures, and antimicrobial spectrums, as well as discussing the structure activity relationships and mechanisms of action of some compounds.
Article
Pharmacology & Pharmacy
Jian feng Mei, Shao fen Cai, Yu Yi, Xu dong Wang, Guo qing Ying
Summary: Serrapeptase has excellent fibrinolytic activity and can be used as a health food or candidate drug for the prevention or treatment of thrombotic diseases.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Biophysics
Yu Yi, Ting Huang, Yunxia Xu, Jianfeng Mei, Yanlu Zhang, Xudong Wang, Jianshu Chen, Guoqing Ying
Summary: Chitosan and its derivatives have great potential for use in wound dressings due to their biocompatibility, biodegradability, antimicrobial activity, hemostatic effects, and wound healing properties. In this study, a drug-loaded hydrogel was prepared by mixing N-glycosylated chitosan with polyvinyl alcohol, and the performance of the hydrogel was evaluated. The blended hydrogel showed excellent antimicrobial properties and wound healing ability.
JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS
(2022)
