Article
Food Science & Technology
Jichao Huang, Kairan Sheng, Yali Zhang, Mengmeng Song, Ahtisham Ali, Tianran Huang, Ming Huang
Summary: This study identified a new sterilization combination consisting of a germinant (AGFNa-1) and cold plasma treatment that effectively deactivated Bacillus licheniformis spores. The combination not only reduced the spore count, but also disrupted the inner membrane structure and inactivated the bacteria.
Article
Biochemistry & Molecular Biology
Charlotte Da Silva, Camille Lamarche, Carole Pichereaux, Emmanuelle Mouton-Barbosa, Gauthier Demol, Sebastien Boisne, Etienne Dague, Odile Burlet-Schiltz, Flavien Pillet, Marie-Pierre Rols
Summary: Low-energy electron beams (LEEB) are a safe and practical sterilization solution for industrial applications. To address the limitations of LEEB, we developed a low-energy pulsed electron beam generator (LEPEB) that can effectively and efficiently eradicate bacteria in a wide range of industrial applications.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Sophie Blinker, Jocelyne Vreede, Peter Setlow, Stanley Brul
Summary: Bacillus subtilis forms dormant spores upon nutrient depletion, with GerA as the major germinant receptor. GerA consists of three subunits and activation by L-alanine requires all three subunits. Structural predictions for the integral membrane protein GerAB indicate it is an alpha-helical transmembrane protein containing a water channel. Free L-alanine binds transiently to specific sites on GerAB in molecular dynamics simulations, providing a starting point for understanding L-alanine mediated signaling by GerAB in spore germination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Juan Wen, Jan P. P. M. Smelt, Norbert O. E. Vischer, Arend L. de Vos, Peter Setlow, Stanley Brul
Summary: The heat activation and heat damage profile of Bacillus subtilis spores were determined in this study. Heat activation at 40 to 70 degrees C promoted spore germination, while heat inactivation and damage to germination-related molecules occurred at 70 degrees C.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Immunology
Joe James, Stephanie M. Meyer, Huynh A. Hong, Chau Dang, Ho T. Y. Linh, William Ferreira, Paidamoyo M. Katsande, Linh Vo, Daniel Hynes, William Love, Ashley C. Banyard, Simon M. Cutting
Summary: This study found that nasal dosing using heat-stable spores can reduce clinical symptoms of influenza infection and decrease viral shedding in animals. This approach may have important implications for influenza prophylaxis in both humans and animals.
Article
Food Science & Technology
Yaru Pang, Ruobin Wu, Tianlin Cui, Zequn Zhang, Li Dong, Fang Chen, Xiaosong Hu
Summary: This study investigated a method of Bacillus subtilis spore inactivation using high pressure, moderate temperature, and the addition of antimicrobial peptide LK. The spores showed cortex hydrolysis and inner membrane damage, leading to a significant reduction in spore counts. Proteomics analysis revealed that the treatment induced changes in the abundance of specific proteins involved in genetic information processing, metabolism, cellular processes, and environmental information processing.
Article
Biotechnology & Applied Microbiology
Jiajia Li, Jie Yang, Weishan Xin, Sirui Wu, Xujuan Wang, Chuanfa Wang, Zhong Zhang
Summary: Combining High-pressure Thermal Treatment (HPTT) and Potassium Sorbate (PS) has a stronger spore inactivation effect. The combination treatment resulted in a higher inactivation rate of Bacillus subtilis spores compared to HPTT treatment alone. It also caused changes in spore permeability, size, inner membrane damage, and reduced enzyme activity. Consequently, the combination of HPTT and PS is proposed as a promising strategy for Bacillus subtilis spore inactivation.
Article
Food Science & Technology
Rosa Heydenreich, Alessia I. Delbruck, Alexander Mathys
Summary: High pressure processing has the potential to inactivate bacterial spores with minimal thermal input. This study used flow cytometry to explore the physiological state of high pressure treated spores, and found that post-treatment conditions significantly influenced the spores' physiological state.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Engineering, Chemical
Yongji Zhang, Yejing Chen, Jie Qiu, Lingling Zhou
Summary: This study demonstrates that the combined treatment of UV irradiation and sodium persulfate can effectively disinfect microorganisms in drinking water. Both UV irradiation and sodium persulfate can individually inactivate bacteria, but the combined treatment method is the most effective.
DESALINATION AND WATER TREATMENT
(2022)
Article
Microbiology
Marketa Husakova, Michaela Plechata, Barbora Branska, Petra Patakova
Summary: The study found that the addition of nitrite alone had no effect on spore germination, but when combined with pickling salt, it effectively inhibited the germination of Clostridium spores. Red yeast rice (RYR) extract was able to completely suppress the germination of Clostridium spores, while having a lesser effect on Bacillus spores.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Rasmus Oberg, Tobias Dahlberg, Dmitry Malyshev, Magnus Andersson
Summary: This study investigates the use of isotope labeling and Raman microscopy as a rapid and cost-effective method for monitoring spore metabolic activity and sterilization verification. The appearance of a Raman peak related to C-D bonds in the spores, along with the observation of the first cell division, suggests low metabolic activity during germination. Additionally, the addition of 30% heavy water did not affect the germination and cell growth rate of the spores.
Article
Food Science & Technology
Si Qin, Minqi Zhou, Zifeng Wang, Pengfei Li, Shihai Huang, Jianzong Meng
Summary: This study investigated the effects of pulsed electric field (PEF) treatment on Aspergillus niger, a source of enzymes, on spore germination and the production of lipase and glucoamylase. The results showed that specific PEF parameters could promote spore germination rate and enzyme activities. Input energy density had a gradual impact on spore germination and enzyme activities, but electric field strength was more decisive.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2023)
Article
Microbiology
Shuang Lu, Xianyin Liao, Li Zhang, Ying Fang, Meixian Xiang, Xiaohua Guo
Summary: The study found that L-alanine is effective in enhancing the probiotic function of Bacillus in animal production by promoting intestinal health, offering a potential alternative to antibiotics in feed.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Food Science & Technology
Lihua Fan, Yanru Zhang, Balarabe Bilyaminu Ismail, Aliyu Idris Muhammad, Guoliang Li, Donghong Liu
Summary: Bacterial spores are resistant to harsh environmental conditions, but can be induced to germinate using specific germinants. The germination-inactivation strategy has been validated in food and has sparked interest in maximizing spore germination. However, there are still uncertainties and unknown signal transduction mechanisms involved in germination. Sensitive detection of germination biomarkers and optimal timing for subsequent inactivation are crucial for the successful implementation of the strategy.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
B. G. Rodriguez-Mendez, A. N. Hernandez-Arias, D. G. Gutierrez-Leon, R. Lopez-Callejas, A. Mercado-Cabrera, B. Jaramillo-Sierra, R. Pena-Eguiluz, R. Valencia-Alvarado, D. Alcantara-Diaz
Summary: This study examined the effects of voltage and oxygen on bacterial inactivation in water using pulsed dielectric barrier discharge under atmospheric pressure. The results showed that there was a minimum voltage requirement for inactivating E. coli and S. typhi samples, with significantly higher inactivation rates observed with the presence of oxygen.
BIOELECTROCHEMISTRY
(2021)
Article
Food Science & Technology
Kwang-Il Kim, Sang-Yoon Lee, Soo-Jin Kim, Jae-Hee Seo, Joong-Kyu Lee, Jung-Kue Shin, Hyung-Yong Cho, Mi-Jung Choi
KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES
(2016)
Article
Food Science & Technology
Yun-hee Hwang, Gorae Kim, Jung-Kue Shin, Seokhoon Lee, Yu Ryang Pyun, Hyung-Yong Cho
FOOD SCIENCE AND BIOTECHNOLOGY
(2017)
Article
Microbiology
Ashagrie Gibtan, Kyounghee Park, Mingyeong Woo, Jung-Kue Shin, Dong-Woo Lee, Jae Hak Sohn, Minjung Song, Seong Woon Roh, Sang-Jae Lee, Han-Seung Lee
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Food Science & Technology
Chan-Ick Cheigh, Mi-Hyun Park, Myong-Soo Chung, Jung-Kue Shin, Young-Seo Park
Article
Engineering, Chemical
Mun-Sil Choi, Chan-Ick Cheigh, Eun-Ae Jeong, Jung-Kue Shin, Myong-Soo Chung
JOURNAL OF FOOD ENGINEERING
(2010)
Article
Food Science & Technology
Seung Young Lee, Won Kyu Lee, Joung Woo Lee, Myong-Soo Chung, Se-Wook Oh, Jung-Kue Shin, Sea C. Min
Summary: The development of a large-scale PPU system for microbial decontamination of particulate foods has shown enhanced microbial inactivation effects, with the maximum reduction achieved at 7 minutes with 200g samples. The PPU treatment synergistically inactivated microorganisms while maintaining the quality properties of rice germ, suggesting its potential use for microbial decontamination while minimizing alterations in quality.
FOOD AND BIOPROCESS TECHNOLOGY
(2021)
Article
Food Science & Technology
Mun-Sil Choi, Chan-Ick Cheigh, Eun-Ae Jeong, Jung-Kue Shin, Ji-Yong Park, Kyung-Bin Song, Jong-Hyun Park, Ki-Sung Kwon, Myong-Soo Chung
FOOD SCIENCE AND BIOTECHNOLOGY
(2009)
Article
Food Science & Technology
Beom-Seok Song, Mi-Jung Kim, Jin-Gyu Park, Jae-Hun Kim, Duk-Jin Kim, Sang-Bae Han, Jung-Kue Shin, Myung-Woo Byun, Ju-Woon Lee
FOOD SCIENCE AND BIOTECHNOLOGY
(2008)
Article
Food Science & Technology
Jung-Kue Shin, Kwan-Jae Jung, Yu-Ryang Pyun, Myong-Soo Chung
FOOD SCIENCE AND BIOTECHNOLOGY
(2007)
Article
Biotechnology & Applied Microbiology
SJ Yoon, MA Yu, GS Sim, ST Kwon, JK Hwang, JK Shin, IH Yeo, YR Pyun
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2002)
