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Biochemistry & Molecular Biology
Joell L. Solan, Sunil R. Hingorani, Paul D. Lampe
Summary: Pancreatic ductal adenocarcinoma (PDA) is characterized by aggressive nature, high metastasis, and strong desmoplasia. Connexin protein Cx43 plays a crucial role in regulating PDA progression through modulation of cell behaviors and gap junction assembly. Mutation in Cx43 phosphorylation sites can significantly impact cancer progression, leading to extended lifespan and reduced metastasis in mouse models. Moreover, stromal gap junctions and associated signaling events mediated by Cx43 may facilitate the metastatic capacity of primary tumors.
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Cell Biology
Vincent Archambault, Jingjing Li, Virginie Emond-Fraser, Myreille Larouche
Summary: In most animal cell types, the disassembly of the interphase nucleus during mitotic entry is well-studied, but the mechanisms of nuclear reassembly at the end of mitosis are less understood. Recent research has made progress in deciphering the roles of dephosphorylation events in promoting nuclear envelope reassembly, chromosome decondensation, kinetochore disassembly, and interphase chromatin organization. The precise roles and regulation of protein phosphatases in this process, particularly the PP1 and PP2A groups, are being elucidated.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
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Biochemistry & Molecular Biology
Kazuhiko S. K. Uchida, Minji Jo, Kota Nagasaka, Motoko Takahashi, Norihisa Shindo, Katsushi Shibata, Kozo Tanaka, Hiroshi Masumoto, Tatsuo Fukagawa, Toru Hirota
Summary: The spindle-assembly checkpoint delays anaphase onset in response to microtubule vacancy at kinetochores. Kinetochore stretching is found to be essential for checkpoint silencing by promoting PP1 phosphatase recruitment. Lower frequency of kinetochore stretching is associated with prolonged metaphase in cancer cell lines with chromosomal instability.
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Biochemistry & Molecular Biology
Babhrubahan Roy, Simon J. Y. Han, Adrienne N. Fontan, Soubhagyalaxmi Jema, Ajit P. Joglekar
Summary: Accurate chromosome segregation during cell division requires proper attachment between the spindle apparatus and the chromosomes. This is ensured by the combined activity of the spindle assembly checkpoint (SAC) and an error correction mechanism. Research has shown that Aurora B kinase directly promotes the production of the mitotic checkpoint complex (MCC) and works downstream of Mps1 in promoting SAC signaling.
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Cell Biology
Luciano Gama Braga, Diogjena Katerina Prifti, Chantal Garand, Pawan Kumar Saini, Sabine Elowe
Summary: Accurate chromosome alignment during metaphase is crucial for proper chromosome segregation in cell division, and tools to measure alignment errors are important for understanding chromosome segregation defects. This study developed and validated a method to quantify chromosome misalignment, which will allow sensitive and systematic analysis of chromosome segregation defects in mitotic cells.
MOLECULAR BIOLOGY OF THE CELL
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Cell Biology
Hiromi Maekawa, Shen Jiangyan, Kaoru Takegawa, Gislene Pereira
Summary: The mitotic exit network (MEN) is important for terminating mitosis in yeasts, but its molecular mechanisms differ between different species.
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Biology
Yuichi Murase, Masahiko Yamagishi, Naoyuki Okada, Mika Toya, Junichiro Yajima, Takahiro Hamada, Masamitsu Sato
Summary: Microtubule shortening is crucial for chromosome movement during cell division. In this study, the researchers demonstrated that Dis1, an ortholog of TOG/XMAP215 in fission yeast, plays a role in promoting microtubule shortening. This finding suggests an unconventional function of Dis1 in driving chromosome movement.
COMMUNICATIONS BIOLOGY
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Cell Biology
Amrita Kumari, Chandan Kumar, Rajaiah Pergu, Megha Kumar, Sagar P. Mahale, Neeraj Wasnik, Sivaram V. S. Mylavarapu
Summary: Mitotic phosphorylation of LIC1-CTD at its three cdk1 sites is essential for proper mitotic progression, dynein loading onto prometaphase kinetochores, and spindle assembly checkpoint inactivation in human cells. Phosphorylation also promotes the binding of Pin1 to Hook2dynein-Nde1-Lis1 complexes, affecting various aspects of mitotic processes.
JOURNAL OF CELL BIOLOGY
(2021)
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Biochemistry & Molecular Biology
Ana Margarida Gomes, Bernardo Orr, Marco Novais-Cruz, Filipe De Sousa, Joana Macario-Monteiro, Carolina Lemos, Cristina Ferras, Helder Maiato
Summary: Defects in chromosome alignment may lead to chromosomal/genomic instability, further driving the division of cancer cells. This study uncovers the distinct mechanism of misaligned chromosomes and reveals genetic conditions predisposing to these events.
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Genetics & Heredity
Majid Nikpay
Summary: This study integrated GWAS data and used Mendelian randomization to identify 107 methylation sites associated with 84 metabolites. The majority of associations were with lipids, which was significantly higher than expected. The study also identified 82 genes that mediate the impact of methylation sites on metabolites and cardiometabolic traits.
FRONTIERS IN GENETICS
(2023)
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Virology
Mackenzie L. Anderson, Owen M. Sullivan, Sarah L. Nichols, Liam Kaylor, Deborah F. Kelly, Sarah McDonald Esstman
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JOURNAL OF VIROLOGY
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Biochemistry & Molecular Biology
John Robert Davis, Anna P. Ainslie, John J. Williamson, Ana Ferreira, Alejandro Torres-Sanchez, Andreas Hoppe, Federica Mangione, Matthew B. Smith, Enrique Martin-Blanco, Guillaume Salbreux, Nicolas Tapon
Summary: This research on Drosophila reveals that the development of adult abdominal epidermis is driven by cell divisions initiated by enzymes secreted by neighboring larval epidermal cells. The study also shows that the development involves changes in the tissue microenvironment and a rapid exit from the cell cycle.
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Cell Biology
Rachel L. Flores, Zachary E. Peterson, Alex Zelter, Michael Riffle, Charles L. Asbury, Trisha N. Davis
Summary: The study reveals that interactions between Ndc80 complex and Dam1 complex through three distinct interacting regions play a vital role in supporting both short-term and long-term tip-coupling during mitosis. Phosphorylation by Aurora B kinase may modulate these interactions and impact the accuracy of mitosis.
JOURNAL OF CELL BIOLOGY
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Cell Biology
Judith Mueller, Monique Furlan, David Settele, Benjamin Grupp, Nils Johnsson
Summary: Muller et al. have discovered the role of septin sumoylation in cell separation. Sumoylated septins recruit the Fir1-Skt5 complex, which stimulates chitin synthase III for septum formation. This study sheds light on the significance of septin sumoylation in cell separation, which was previously unclear.
JOURNAL OF CELL BIOLOGY
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Genetics & Heredity
Majid Nikpay, Sepehr Ravati, Ruth McPherson
Summary: This study identified DNA methylation sites that impact the proteome and made the results available as freeware for biological insight. The research demonstrated that epigenomic modifications at DNA sites can influence protein levels, with methylation of certain sites contributing to the onset of diseases.
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Biochemistry & Molecular Biology
Ryan A. Henry, Yin-Ming Kuo, Vikram Bhattacharjee, Timothy J. Yen, Andrew J. Andrews
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Oncology
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CANCER BIOLOGY & THERAPY
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Neil Beeharry, Jerome B. Rattner, Juliane P. Caviston, Tim Yen
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Neil Beeharry, Eugenia Banina, James Hittle, Natalia Skobeleva, Vladimir Khazak, Sean Deacon, Mark Andrake, Brian L. Egleston, Jeffrey R. Peterson, Igor Astsaturov, Timothy J. Yen
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V. Bhattacharjee, Y. Zhou, T. J. Yen
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Biochemistry & Molecular Biology
W. C. Earnshaw, R. C. Allshire, B. E. Black, K. Bloom, B. R. Brinkley, W. Brown, I. M. Cheeseman, K. H. A. Choo, G. P. Copenhaver, J. G. DeLuca, A. Desai, S. Diekmann, S. Erhardt, M. Fitzgerald-Hayes, D. Foltz, T. Fukagawa, R. Gassmann, D. W. Gerlich, D. M. Glover, G. J. Gorbsky, S. C. Harrison, P. Heun, T. Hirota, L. E. T. Jansen, G. Karpen, G. J. P. L. Kops, M. A. Lampson, S. M. Lens, A. Losada, K. Luger, H. Maiato, P. S. Maddox, R. L. Margolis, H. Masumoto, A. D. McAinsh, B. G. Mellone, P. Meraldi, A. Musacchio, K. Oegema, R. J. O'Neill, E. D. Salmon, K. C. Scott, A. F. Straight, P. T. Stukenberg, B. A. Sullivan, K. F. Sullivan, C. E. Sunkel, J. R. Swedlow, C. E. Walczak, P. E. Warburton, S. Westermann, H. F. Willard, L. Wordeman, M. Yanagida, T. J. Yen, K. Yoda, D. W. Cleveland
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Biochemistry & Molecular Biology
Allison M. Michal, Christopher H. So, Neil Beeharry, Haripriya Shankar, Rouzbeh Mashayekhi, Timothy J. Yen, Jeffrey L. Benovic
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Biochemistry & Molecular Biology
Kexi Wang, Brianne Sturt-Gillespie, James C. Hittle, Dawn Macdonald, Gordon K. Chan, Tim J. Yen, Song-Tao Liu
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Liang Hu, Harilaos Filippakis, Haomin Huang, Timothy J. Yen, Ole V. Gjoerup
JOURNAL OF VIROLOGY
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Cell Biology
Matthew K. Martz, Elda Grabocka, Neil Beeharry, Timothy J. Yen, Philip B. Wedegaertner
MOLECULAR BIOLOGY OF THE CELL
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Gregor M. Balaburski, Julia I. -Ju Leu, Neil Beeharry, Seth Hayik, Mark D. Andrake, Gao Zhang, Meenhard Herlyn, Jessie Villanueva, Roland L. Dunbrack, Tim Yen, Donna L. George, Maureen E. Murphy
MOLECULAR CANCER RESEARCH
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Biochemistry & Molecular Biology
V. Ratushny, H. B. Pathak, N. Beeharry, N. Tikhmyanova, F. Xiao, T. Li, S. Litwin, D. C. Connolly, T. J. Yen, L. M. Weiner, A. K. Godwin, E. A. Golemis
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Esther Eytan, Kexi Wang, Shirly Miniowitz-Shemtov, Danielle Sitry-Shevah, Sharon Kaisari, Tim J. Yen, Song-Tao Liu, Avram Hershko
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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Haomin Huang, Michael Lampson, Andrey Efimov, Timothy J. Yen