Article
Biochemistry & Molecular Biology
Wenjuan Yu, Ramesh Singh, Zhao Wang, Bert W. O'Malley, Ping Yi
Summary: TRAF4-mediated ubiquitination of IRS-1 is physiologically relevant and crucial for IGF signal transduction, as it promotes an atypical K29-linked ubiquitination at the C-terminal end of IRS-1, affecting downstream AKT and ERK phosphorylation and breast cancer cell proliferation. The nonproteolytic ubiquitination of IRS-1 is a key step in conveying IGF-1 stimulation from IGFR to IRS-1.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Oncology
Liangzi Cao, Hao Li, Xiaofang Liu, Yubang Wang, Bowen Zheng, Chengzhong Xing, Naijin Zhang, Jingwei Liu
Summary: This study investigated the molecular alterations and clinical relevance of NEDD4 E3 ligase family genes in 33 cancer types. The findings revealed increased expression of NEDD4 members in pancreatic cancer and decreased expression in thyroid cancer. NEDD4 family genes exhibited a range of mutation frequencies, with HECW1 and HECW2 showing relatively high mutation rates. Breast cancer presented with a high frequency of NEDD4 copy number amplification. Additionally, NEDD4 family members were involved in various pathways such as p53, Akt, apoptosis, and autophagy, and their expression correlated with the survival of cancer patients.
Article
Biotechnology & Applied Microbiology
Yuan Qi, Yan Tang, Lu Yin, Keke Ding, Cuimei Zhao, Wenwen Yan, Yi'an Yao
Summary: In this study, it was found that miR-129-5p targeted Smurf1 to inhibit PTEN ubiquitination and enhance PTEN expression, thereby improving cardiac function in chronic heart failure rats.
Article
Biochemistry & Molecular Biology
Anirban Dasgupta, Payel Mondal, Sambit Dalui, Chandrima Das, Siddhartha Roy
Summary: The plant homeodomain (PHD) finger of UBR7 is essential for its association with E2 UbcH6 and consequent ubiquitin transfer to histone H2B. The mechanism of ubiquitin transfer by UBR7 differs markedly from that of RNF20.
Article
Pharmacology & Pharmacy
Shu-Ze Zheng, Xiao-Wen Zhang, Xiao-Min Song, Zhuo Yang, Lu Yao, Peng-Fei Tu, Ke-Wu Zeng
Summary: This study discovered a natural small-molecule compound ECL that inhibits microglia-mediated neuroinflammation by editing the monoubiquitination of histone H2B. ECL modifies the previously undisclosed K46 site in H2B and recruits the E3 ubiquitin ligase to promote H2Bub1 at K120, leading to disruption of AP-1 binding to proinflammatory genes in microglia.
PHARMACOLOGICAL RESEARCH
(2022)
Review
Immunology
Luiz Pedro Souza-Costa, Josiane Teixeira Andrade-Chaves, Juvana Moreira Andrade, Vivian Vasconcelos Costa, Luis Henrique Franco
Summary: Innate immunity is the body's first line of defense against infections, but uncontrolled or aberrant inflammatory responses can lead to tissue damage and chronic inflammatory diseases. This review focuses on the importance of ubiquitination in regulating innate immune signaling and inflammation, highlighting the role of the protein Smurf1 and its potential as a therapeutic target for infectious and inflammatory conditions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Kelong Ma, Rundong Shu, Hongtao Liu, Jiaqi Fu, Zhao-Qing Luo, Jiazhang Qiu
Summary: Legionella pneumophila, a pathogenic bacteria, can create an environment conducive to its replication by exploiting host cell signaling pathways and the ubiquitin system. In this study, we identified a novel effector protein, Lug15, which functions as an E3 ligase and remodels the Legionella phagosome.
Article
Plant Sciences
Bo Lu, Jianhua Li, MingTai Gui, Lei Yao, Mingsong Fan, Xunjie Zhou, Deyu Fu
Summary: SalB protects cardiomyocytes from I/R-induced injury by regulating the TRIM8/GPX1 axis. TRIM8 overexpression promotes cardiomyocyte injury, which can be alleviated by SalB. In addition, TRIM8 negatively regulates the expression of the antioxidant enzyme GPX1.
PHARMACEUTICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anubrita Das, Martina Foglizzo, Prasanth Padala, Jingyi Zhu, Catherine L. Day
Summary: In this study, direct evidence is provided to support the essential role of TRAF trimers in signaling. The study demonstrates that TRAF6 trimers bind Lys63-linked ubiquitin chains to promote their processive assembly.
Article
Biochemistry & Molecular Biology
Me-Sun Kim, Seo-Rin Ko, Yu Jin Jung, Kwon-Kyoo Kang, Yung-Jo Lee, Yong-Gu Cho
Summary: Plants produce stress-resistant substances in response to abiotic stress, and a protein conversion mechanism helps break down damaged proteins. The study focused on the function of OsPUB7 gene in Oryza sativa and found that OsPUB7 plays a role in drought and salinity stress resistance. The interaction between OsPUB7 and other stress-related genes suggests its potential as a target for breeding and future research on drought tolerance in rice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Hongbiao Ran, Chunyan Li, Ming Zhang, Jincheng Zhong, Hui Wang
Summary: Ubiquitination, a post-transcriptional modification, plays a crucial role in protein degradation and is involved in various physiological and pathological processes, including animal adipogenesis. The E3 ligases have been found to regulate adipogenesis, but their role in the recognition and connection between substrate and ubiquitin during ubiquitination has been overlooked. This review summarizes the regulatory and modification targets of E3 ligases in animal adipogenesis, explains the regulatory mechanisms in lipogenic pathways, and analyzes existing positive results, providing valuable research directions for further understanding the regulatory mechanisms of E3 ligases in animal adipogenesis.
Article
Biochemistry & Molecular Biology
Huihui Liu, Simiao Liu, Hong Yu, Xiahe Huang, Yingchun Wang, Liang Jiang, Xiangbing Meng, Guifu Liu, Mingjiang Chen, Yanhui Jing, Feifei Yu, Bing Wang, Jiayang Li
Summary: In this study, the authors characterized and reconstituted the rice SCF E3 ligase in vitro, and also developed an engineered system with interchangeable F-box proteins. This work provides a powerful platform for studying the mechanisms of multisubunit SCF E3 ligases in eukaryotes.
Article
Biochemistry & Molecular Biology
Busra Turgu, Amal El-Naggar, Melanie Kogler, Luigi Tortola, Hai-Feng Zhang, Mariam Hassan, Michael M. Lizardo, Sonia H. Y. Kung, Wan Lam, Josef M. Penninger, Poul H. Sorensen
Summary: HACE1 destabilizes mTOR by targeting RAC1 within mTOR-associated complexes, leading to decreased mTORC1 and mTORC2 activity. HACE1 deficiency results in enhanced mTORC1/2 activity, which can be reversed by inhibiting RAC1. These findings reveal a unique ubiquitin-dependent process to control the activity of mTOR signaling complexes.
Article
Biochemistry & Molecular Biology
Zhiheng Deng, Huasong Ai, Maoshen Sun, Zebin Tong, Yunxiang Du, Qian Qu, Liying Zhang, Ziyu Xu, Shixian Tao, Qiang Shi, Jia-Bin Li, Man Pan, Lei Liu
Summary: This study elucidates the mechanism of H2B monoubiquitylation and highlights the critical role of nucleosomal DNA in mediating E3 ligase recognition.
Article
Chemistry, Multidisciplinary
Francesca Munari, Luca Mollica, Carlo Valente, Francesca Parolini, Elham Ataie Kachoie, Giorgio Arrigoni, Mariapina D'Onofrio, Stefano Capaldi, Michael Assfalg
Summary: The study reveals that E3 ligase CHIP can ubiquitinate Tau protein at multiple sites, even in the absence of molecular chaperones, providing mechanistic insight into the chaperone-independent engagement of a disordered protein by its E3 ligase.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Cell Biology
Ying E. Zhang
CURRENT OPINION IN CELL BIOLOGY
(2018)
Article
Immunology
Eric Tu, Cheryl P. Z. Chia, Weiwei Chen, Dunfang Zhang, Sang A. Park, Wenwen Jin, Dandan Wang, Maria-Luisa Alegre, Ying E. Zhang, Lingyun Sun, WanJun Chen
Article
Biochemistry & Molecular Biology
Yi Tang, Liu-Ya Tang, Xuan Xu, Cuiling Li, Chuxia Deng, Ying E. Zhang
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2018)
Article
Biochemistry & Molecular Biology
Veenu Tripathi, Jee-Hye Shin, Christina H. Stuelten, Ying E. Zhang
Article
Biochemistry & Molecular Biology
Kun Zhu, Yi Tang, Xuan Xu, Hien Dang, Liu-Ya Tang, Xiang Wang, Xin Wei Wang, Ying E. Zhang
Article
Multidisciplinary Sciences
Erine H. Budi, Steven Hoffman, Shaojian Gao, Ying E. Zhang, Rik Derynck
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Frank Stappenbeck, Feng Wang, Liu-Ya Tang, Ying E. Zhang, Farhad Parhami
Article
Cell Biology
Ji-Hyeon Lee, Jose Manuel Mellado-Gil, Young Jae Bahn, Sushrut M. Pathy, Ying E. Zhang, Sushil G. Rane
CELL DEATH & DISEASE
(2020)
Article
Biochemistry & Molecular Biology
Miao Yu, Xiaoyan Shi, Mengmeng Ren, Lu Liu, Hao Qi, Chi Zhang, Junhua Zou, Xiaoyan Qiu, Wei-Guo Zhu, Ying E. Zhang, Wengong Wang, Jianyuan Luo
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Liu-Ya Tang, Adam Thomas, Ming Zhou, Ying E. Zhang
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Review
Cell Biology
Christina H. Stuelten, Ying E. Zhang
Summary: TGF-beta plays a dual role in tumors, acting as an inhibitor of early-stage tumorigenesis while promoting late-stage tumor progression and metastasis. Through its ability to alter cell differentiation, TGF-beta induces transitions of various cell types, contributing to the progression of epithelial tumors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Feng Wang, Frank Stappenbeck, Liu-Ya Tang, Ying E. Zhang, Simon T. Hui, Aldons J. Lusis, Farhad Parhami
Summary: Inflammatory responses are crucial for protecting against infections and maintaining health. Many diseases involve chronic inflammation, but current anti-inflammatory drugs often have adverse effects for long-term use. Therefore, the development of effective and non-toxic anti-inflammatory drugs is important. A semi-synthetic oxysterol called Oxy210 and its analogs have been found to exhibit anti-inflammatory effects in both animal models and cell experiments, making them potential candidates for future therapeutic development against inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Liu-Ya Tang, Marie Spezia, Ting Chen, Jee-Hye Shin, Feng Wang, Frank Stappenbeck, Andres M. Lebensohn, Farhad Parhami, Ying E. Zhang
Summary: The study revealed that oxysterols Oxy186 and Oxy210 can inhibit proliferation of NSCLC cells, with Oxy186 exhibiting potent inhibition of tumor growth. The mechanism of action involves inhibiting the WNT/beta-catenin signaling pathway.
CELL AND BIOSCIENCE
(2022)
Article
Pathology
Christina H. Stuelten, Nicolas Melis, Bhagawat Subramanian, Yi Tang, Megan Kimicata, John P. Fisher, Roberto Weigert, Ying E. Zhang
Summary: This study found that Smurf2 plays an important role in cutaneous wound healing. Loss of Smurf2 exacerbates early inflammation in the wounds and leads to narrower wounds but with greater breaking strength. In addition, loss of Smurf2 also increases the linearization of collagen bundles in normal and wounded skin.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Multidisciplinary Sciences
Sang-A Park, Yun-Ji Lim, Wai Lim Ku, Dunfang Zhang, Kairong Cui, Liu-Ya Tang, Cheryl Chia, Peter Zanvit, Zuojia Chen, Wenwen Jin, Dandan Wang, Junji Xu, Ousheng Liu, Fu Wang, Alexander Cain, Nancy Guo, Hiroko Nakatsukasa, Chuan Wu, Ying E. Zhang, Keji Zhao, WanJun Chen
Summary: This study reveals that the differentiation of IL-9-producing Th9 cells is regulated by TGF-beta and IL-4 signals through pSmad3L-Ser(213), and the transcription factors DBP and E2F8 have opposing effects on Il9 gene transcription.
NATURE COMMUNICATIONS
(2022)