Article
Chemistry, Medicinal
Lisa J. Alcock, Yunchao Chang, Jamie A. Jarusiewicz, Marisa Actis, Stanley Nithianantham, Anand Mayasundari, Jaeki Min, Dylan Maxwell, Jeremy Hunt, Brandon Smart, Jun J. Yang, Gisele Nishiguchi, Marcus Fischer, Charles G. Mullighan, Zoran Rankovic
Summary: The study describes the design and synthesis of JAK2/3 PROTACs and their potency in patient-derived ALL cells. SJ10542 showed high selectivity and potency, making it a promising candidate for the treatment of hematological malignancies.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Pharmacology & Pharmacy
N. P. Syamprasad, Nitin Madje, Jayasri Bachannagari, Arun Kumar Jannu, Siddhi Jain, Kalyani Tene, P. A. Shantanu, V. G. M. Naidu, Naveen Chella
Summary: The current therapy for gastrointestinal stromal tumors is limited in its efficacy and resistance. In this study, the potential of the signal transducer and activator of transcription 3 (STAT3) inhibitor, niclosamide, to treat gastrointestinal stromal tumors was explored. Nanocrystals of niclosamide were developed to improve solubility and bioavailability. The study demonstrated that niclosamide induced dose-dependent cytotoxicity, mitochondrial damage, and apoptotic-like cell death in gastrointestinal stromal tumor cells. Niclosamide nanocrystals showed enhanced anticancer activity compared to pure niclosamide. Furthermore, niclosamide nanocrystals exhibited better tumor suppression, indicating higher bioavailability. This study suggests that nanocrystals could be a promising delivery strategy for niclosamide in treating gastrointestinal stromal tumors.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Maria S. M. Mendes, Marguerita E. Rosa, Joao A. P. Coutinho, Mara G. Freire, Francisca A. e Silva
Summary: This study proposes a method for pretreating human serum using aqueous biphasic systems to improve the accuracy of pentraxin-3 analysis. The research reveals that a system composed of polyethylene glycol is capable of efficiently depleting high abundance proteins and extracting biomarkers from human serum, further validating its potential as a diagnosis/prognosis biomarker.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Xiangshan Xu, Yang Yang, Guofeng Wang, Yu Yin, Shuo Han, Donghan Zheng, Shaobo Zhou, Yuanyuan Zhao, Yong Chen, Yuanzhe Jin
Summary: The study revealed that low shear stress can suppress the expression of mechanosensitive miR-181b-5p in HUVECs, leading to an increase in NLRP3 inflammasome-dependent pyroptosis. Inhibition of STAT-3 gene expression at the posttranscriptional level can alleviate NLRP3 inflammasome-dependent pyroptosis. Regulation of the expression levels of mechanosensitive miR-181b-5p and STAT-3 may be a potential strategy to control atherosclerosis induced by shear stress.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Iqra Batool, Naim Bajcinca
Summary: In this paper, a mathematical analysis is performed on a proposed nonlinear, multiscale mathematical model that describes the dynamics of cell populations and cell-cycle proteins. The model shows the impact of cell cycle dynamics on the evolution of cell population in a tissue. The well-posedness of the model is studied, steady-state solutions are derived, and conditions for the stability of steady-state solutions are found. Numerical simulations are performed to understand the effects of parameters on the model's nonlinear dynamics.
Article
Biochemistry & Molecular Biology
Ming Zhang, Jianmei Lai, Qianlong Wu, Jia Lai, Jingyao Su, Bing Zhu, Yinghua Li
Summary: Hepatocarcinoma is a common digestive system tumor worldwide with no effective treatment. Naringenin, isolated from citrus fruits, has shown anticancer effects. This study investigated the molecular mechanisms and oxidative stress implications of naringenin-induced cytotoxicity in HepG2 cells. Naringenin induced apoptosis in HepG2 cells through multiple pathways, enhanced cytotoxic effects, triggered reactive oxygen species, inhibited JAK-2/STAT-3 signaling pathways, and activated caspase-3. These findings suggest that naringenin could be a promising candidate for cancer therapy.
Article
Oncology
Giulia Veltri, Federica Lovisa, Giuliana Cortese, Marta Pillon, Elisa Carraro, Simone Cesaro, Massimo Provenzi, Salvatore Buffardi, Samuela Francescato, Alessandra Biffi, Barbara Buldini, Valentino Conter, Valentina Serafin, Lara Mussolin
Summary: T-cell lymphoblastic lymphoma (T-LBL) and lymphoblastic leukemia (T-ALL) share common features but have different phosphoproteomic profiles. A proteomic signature of six proteins can distinguish stage IV T-LBL from T-ALL, and AKT hyperphosphorylation alone can distinguish stage IV T-LBL from both T-ALL and stage III T-LBL.
FRONTIERS IN ONCOLOGY
(2022)
Article
Electrochemistry
Dipika Meghnani, Rajendra Kumar Singh
Summary: In this study, a biphase O'3/O3 layered NaNi0.815Co0.15Al0.035O2 cathode material (Na-NCA) is synthesized using different chemical routes. The Na-NCA cathode prepared using hydrothermal method shows better sodium ion kinetics and electrochemical performance compared to other methods.
ELECTROCHIMICA ACTA
(2022)
Article
Immunology
Zhenpeng Dai, Tanya Sezin, Yuqian Chang, Eunice Y. Lee, Eddy Hsi Chun Wang, Angela M. Christiano
Summary: This study found that the JAK inhibitor Ifidancitinib can effectively promote hair regrowth in AA mice. Ifidancitinib can alleviate AA-associated inflammation and reduce the number of T cells associated with the pathogenesis of AA. The study also found that the expression of co-inhibitory receptor PD-1 on effector/memory CD8(+) T cells increased, while IFN-gamma production decreased in Ifidancitinib-treated mice. The study suggests that selective induction of T cell exhaustion using a JAK inhibitor may be one of the mechanisms underlying the success of this treatment strategy in reversing autoimmune diseases such as AA.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Y. Song, D. H. Yoon, H. Yang, J. Cao, D. Ji, Y. Koh, H. Jing, H. Eom, J. Kwak, W. Lee, J. Lee, H. Shin, J. Jin, M. Wang, Z. Yang, W. S. Kim, J. Zhu
Summary: Golidocitinib, an oral selective JAK1 inhibitor, demonstrates promising antitumor activity in patients with r/r PTCLs. It shows acceptable safety profile and tolerability in this phase I study, supporting its further investigation as a potential treatment option for r/r PTCLs.
ANNALS OF ONCOLOGY
(2023)
Article
Pharmacology & Pharmacy
Dindyal Mandal, Sandeep Lohan, Muhammad Imran Sajid, Abdulelah Alhazza, Rakesh Kumar Tiwari, Keykavous Parang, Hamidreza Montazeri Aliabadi
Summary: In this study, several linear peptides containing tryptophan (W) and arginine (R) residues were designed and synthesized. These peptides were attached to lipophilic fatty acyl chains, cholesterol, or PEG. The newly developed peptides efficiently protected siRNA against early degradation and successfully delivered siRNA into cells, resulting in significant downregulation of target proteins.
Article
Biochemical Research Methods
Lisa Janssen, Gabriele Sadowski, Christoph Brandenbusch
Summary: The main challenge in implementing highly efficient multi-phase whole-cell biocatalytic processes industrially is the formation of stable Pickering-type emulsions, which hinders downstream processing. By using the phenomenon of catastrophic phase inversion (CPI), efficient phase separation can be achieved within minutes. To demonstrate the applicability of CPI as an innovative process step, a fully automated lab-scale prototype was designed and constructed. The knowledge of the CPI point is crucial for successfully destabilizing the inverted state of emulsion.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Hae-in Lee, Hui-Ju Kim, Sungsin Jo, Seung Cheol Shim, Tae-Hwan Kim, Eun Jeong Won, Tae-Jong Kim
Summary: Ankylosing spondylitis (AS) is a chronic inflammatory rheumatic disease characterized by increased Th17 cells. IL-6 plays a crucial role in regulating inflammation and regulatory T cells in AS.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Agricultural Engineering
Qiang Chen, Mingzhao Guo, Yuexin Bi, Ge Qu, Zhoutong Sun, Yujun Wang, Guangsheng Luo
Summary: This study achieved efficient synthesis of S-(4-chlorophenyl)-(pyridin-2-yl) methanol (S-CPMA) by using recombinant Escherichia coli as a whole-cell catalyst and developing a microreaction system. This approach reduced the reaction time and increased the yield by over 70%.
BIORESOURCE TECHNOLOGY
(2021)
Article
Pathology
Michael R. McCarthy, Christin A. Tiegs-Heiden, Charles D. Sturgis, Amy A. Swanson, Sounak Gupta, Judith Jebastin Thangaiah, Christopher P. Hartley
Summary: Biphasic squamoid alveolar renal cell carcinoma is a rare variant of papillary renal cell carcinoma with distinct histomorphology and immunophenotype. The cytomorphologic features of this type of carcinoma have not been described. This study correlates the cytologic features with histomorphology and immunophenotype, providing new insights into this disease.
ANNALS OF DIAGNOSTIC PATHOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Kumuda C. Das, Harish Muniyappa, Venkatesh Kundumani-Sridharan, Jaganathan Subramani
Summary: Research has shown that cancer cells overexpressing thioredoxin (Trx) exhibit enhanced apoptosis in response to daunomycin, while cells overexpressing redox-inactive mutant Trx were not effectively killed. Furthermore, increased levels of Trx specifically potentiate anthracycline toxicity, but not with other topoisomerase II inhibitors such as etoposide.
CARDIOVASCULAR TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Monoranjan Boro, Suresh Govatati, Raj Kumar, Nikhlesh K. Singh, Prahalathan Pichavaram, James G. Traylor, A. Wayne Orr, Gadiparthi N. Rao
Summary: This study revealed the mechanism of thrombin-induced depletion of ABCA1 levels, showing that CSN3 plays a crucial role in protecting ABCA1 from degradation, thus promoting cholesterol efflux and suppressing foam cell formation. Depletion of CSN3 levels in WD-fed ApoE(-/-)mice significantly lowered ABCA1 levels and exacerbated atherosclerotic plaque formation, highlighting the potential anti-atherogenic role of CSN3.
CELL DEATH AND DIFFERENTIATION
(2021)
Correction
Biochemistry & Molecular Biology
Monoranjan Boro, Suresh Govatati, Raj Kumar, Nikhlesh K. Singh, Prahalathan Pichavaram, James G. Traylor, A. Wayne Orr, Gadiparthi N. Rao
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Hematology
Venkatesh Kundumani-Sridharan, Jaganathan Subramani, Cade Owens, Kumuda C. Das
Summary: The study identified Nrg1 beta as a RIPC factor that interacts with endothelial ErbB2 to prevent degradation and decrease myocardial apoptosis through other molecular pathways. This finding is crucial for understanding the protective mechanism of RIPC.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Jaganathan Subramani, Venkatesh Kundumani-Sridharan, Kumuda C. Das
Summary: In the context of myocardial ischemia-reperfusion injury, it has been discovered that SG-eNOS undergoes degradation through a specific mechanism, preventing further tissue damage but also causing irreversible loss of eNOS and NO availability.
CIRCULATION RESEARCH
(2021)
Review
Chemistry, Multidisciplinary
Rahul Sharma, Deepti Sharma, Linda D. Hazlett, Nikhlesh K. Singh
Summary: Nanoscience and nanotechnology have revolutionized environmental sciences, particularly in the field of ocular tissue engineering and regenerative ophthalmology. The use of innovative nanomaterials offers promising prospects for improving eye health, but there are challenges and future directions that should be considered for further development in this area.
Review
Biochemistry & Molecular Biology
Geetika Kaur, Nikhlesh K. Singh
Summary: Retinal neurodegeneration, commonly manifested as apoptosis or impaired function of photoreceptors, is a major cause of irreversible vision loss and blindness. This review summarizes the extensive research conducted on the pathogenesis and treatment of retinal degenerative diseases, including age-related macular degeneration, inherited retinal dystrophies, retinitis pigmentosa, Stargardt macular dystrophy, and Leber congenital amaurosis. The role of inflammation in retinal degeneration is highlighted, and various therapeutic approaches, such as gene therapy and stem cell-based therapies, are evaluated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Correction
Biochemistry & Molecular Biology
Sivareddy Kotla, Nikhlesh K. Singh, Daniel Kirchhofer, Gadiparthi N. Rao
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Correction
Biochemistry & Molecular Biology
Sivareddy Kotla, Nikhlesh K. Singh, Gadiparthi N. Rao
Article
Biology
Deepti Sharma, Shivantika Bisen, Geetika Kaur, Eric C. Van Buren, Gadiparthi N. Rao, Nikhlesh K. Singh
Summary: This study discovered that genetic deletion or siRNA-mediated downregulation of IL-33 can reduce pathological neovascularization in a mouse model of oxygen-induced retinopathy without affecting normal retinal repair. It was also found that IL-33 induces angiogenesis through the NFkappaB-Jagged1-Notch1 signaling pathway. Additionally, IL-33 interacts with BRCA1-associated protein 1 (BAP1) and Numb to enhance the de-ubiquitination and stabilization of Notch1 intracellular domain.
COMMUNICATIONS BIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Geetika Kaur, Shailendra Kumar Verma, Deepak Singh, Nikhlesh K. Singh
Summary: Cell signaling is crucial for cell survival and tolerance towards stressful conditions. It involves receptor molecules that sense and receive signals, such as hormones, light, antigens, odorants, and neurotransmitters. Heterotrimeric G-proteins play a key role in transmitting signals from G-protein-coupled receptors (GPCRs) to control biochemical activities within cells. This review article discusses the functions and regulation of G-protein signaling and their importance in cardiovascular diseases.
BIOENGINEERING-BASEL
(2023)
Review
Pharmacology & Pharmacy
Anamika Sharma, Nikhlesh K. K. Singh
Summary: Retinopathy refers to eye disorders caused by damage to the retina's vascular system, leading to vision loss and sometimes blindness. Recent advancements in high-throughput sequencing have facilitated the discovery of lncRNAs, which are critical regulators of biological processes. Bioinformatics has identified lncRNAs that may have a role in retinal disorders, and using lncRNA transcripts for diagnosis and therapy can provide long-term benefits compared to traditional medicines. This article discusses different lncRNAs that influence various retinopathies and how they can be utilized for identification and treatment.
Article
Biology
Geetika Kaur, Deepti Sharma, Shivantika Bisen, Chandra Sekhar Mukhopadhyay, Katherine Gurdziel, Nikhlesh K. K. Singh
Summary: Vascular adhesion molecules, specifically VCAM-1, have been found to play a crucial role in retinal neovascularization and can be regulated by IL-33. The study also highlights the regulatory role of VCAM-1-JunB-IL-8 signaling in retinal endothelial cell sprouting and angiogenesis.
COMMUNICATIONS BIOLOGY
(2023)