Article
Multidisciplinary Sciences
Parker Smith, Martin Schuster
Summary: Quorum sensing is a widespread cell density-dependent signaling mechanism in bacteria. This study demonstrates the role of antiactivators in preventing self-sensing in the quorum sensing system of Pseudomonas aeruginosa.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Sahana Vasudevan, Parthasarathy Srinivasan, Prasanna Neelakantan, John Bosco Balaguru Rayappan, Adline Princy Solomon
Summary: In this study, a novel photoluminescence-based assay using cysteamine-capped titanium oxide nanoparticles was developed for the rapid and sensitive diagnosis of microbial infections. The system showed a maximum relative response of 98% for AHLs produced by Pseudomonas aeruginosa, with a linear detection range of 10-160 nM in simulated host urine. The efficacy and specificity of the system in detecting AHLs produced by P. aeruginosa were confirmed in a temporal manner.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ankita Chopra, Dattatray Mongad, Surekha Satpute, Pranab Behari Mazumder, Praveen Rahi
Summary: The study aimed to isolate and characterize tea rhizobacteria that produce quorum sensing molecules and exhibit multiple plant growth-promoting activities. Thirty-one isolates were obtained from the tea rhizosphere, and two isolates (RTE1 and RTE4) were selected for their ability to inhibit tea fungal pathogens and produce AHL molecules. Genome-based analysis confirmed RTE1 as Burkholderia cepacia and RTE4 as Pseudomonas aeruginosa. Detailed characterization of the AHLs produced by the isolates revealed different chain lengths. Confocal microscopy showed biofilm formation by RTE1 and RTE4. Several genes involved in quorum sensing, plant growth promotion, and inducing systemic resistance were identified. Based on their functional traits, B. cepacia RTE1 and P. aeruginosa RTE4 are promising candidates for enhancing the health and growth of tea plantations.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Raman Pachaiappan, Tharun Prasanna Rajamuthu, Ananya Sarkar, Pradiksha Natrajan, Nagasathiya Krishnan, Meenakumari Sakthivelu, Palaniyandi Velusamy, Palaniappan Ramasamy, Subash C. B. Gopinath
Summary: Flavonoids and isoflavonoids are effective inhibitors of biofilm formation and virulence factor production in Pseudomonas aeruginosa, with acacetin exhibiting the strongest inhibition capacity. These compounds downregulate the expression of virulence genes, making them potential targets for anti-quorum sensing strategies.
PROCESS BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Kyle E. Harvey, Shiqi Tang, Emily K. LaVigne, Evan P. S. Pratt, Gregory H. Hockerman
Summary: In this study, the researchers found that RyR2 is required for the regulation of insulin content and glucose-stimulated insulin secretion in INS-1 cells, partly through its interaction with IRBIT. The experiments showed that the absence of RyR2 led to a decrease in store-operated Ca2+ entry and PLC activity, as well as an increase in cellular PIP2 levels. Furthermore, RyR2 was found to regulate beta-cell electrical activity by controlling Ca-v current density and SK channel activation.
Article
Medicine, Research & Experimental
V. S. Cherepushkina, T. E. Mironova, V. N. Afonyushkin, V. Yu Koptev, E. Nefedova, N. A. Donchenko, A. S. Dimova
Summary: The study investigated the impact of early accumulation of N-3-oxo-dodecanoyl-homoserine lactone on Pseudomonas aeruginosa reproduction, biofilm formation, and elastase activity. It was found that at a concentration of 25 μM, the lactone slowed down bacterial cell proliferation and promoted biofilm formation, while at a concentration of 0.75 μM, elastase activity was significantly increased.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Flavio Ballante, Maria V. Turkina, Maria Ntzouni, Karl-Eric Magnusson, Elena Vikstrom
Summary: Quorum sensing (QS) is a cell-cell communication system used by bacteria to sense population density and regulate collective behaviors. In this study, four new AHL analogs were designed and evaluated as quorum-sensing inhibitors (QSIs). The compounds were found to reduce the production of signal molecules and certain virulence traits in Pseudomonas aeruginosa.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Review
Microbiology
Yobouet Ines Kouakou, Robert J. Lee
Summary: Bitter and sweet taste receptors, initially found in taste cells on the tongue, have been identified in cells throughout the body, indicating a broader chemosensory role. These receptors regulate various bodily processes and are also used by mammalian cells to monitor bacterial communications. They report information about microbial population density based on the chemical composition of the extracellular environment. This review summarizes current knowledge on bacterial activation of taste receptors and highlights remaining important questions in this field.
Review
Gastroenterology & Hepatology
Jean-Paul Motta, John L. Wallace, Andre G. Buret, Celine Deraison, Nathalie Vergnolle
Summary: This article discusses the natural organization of microorganisms on gastrointestinal surfaces, microbiota taxonomy, biogeographical localization, and trans-kingdom interactions within the biofilm habitat. The importance of the host-biofilm relationship in gut homeostasis and disease is evaluated, along with the role of biofilms in gut homeostasis and pathological processes. Further research on biofilm properties and communication could provide insights into gut physiology and potential therapeutic options for gastrointestinal diseases.
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY
(2021)
Article
Biophysics
Madison Rizzo, Eric Baggs, Abu Sayeed Chowdhury, Rajesh Nagarajan, Lisa Rose Warner
Summary: The N-acyl-L-homoserine lactone (AHL) quorum sensing is important for regulating virulence in Pseudomonas aeruginosa. LasI and RhlI AHL synthases use acyl carrier proteins to synthesize specific QS signals. Only ACP(1) carrier protein is regulated by quorum sensing in P. aeruginosa. In this study, the backbone resonance assignments of ACP(1) were determined to understand its role in AHL quorum sensing signal synthesis.
BIOMOLECULAR NMR ASSIGNMENTS
(2023)
Review
Chemistry, Multidisciplinary
Ivan Tochitsky, Michael A. Kienzler, Ehud Isacoff, Richard H. Kramer
Article
Biochemistry & Molecular Biology
Tong Xiao, Cheri M. Ackerman, Elizabeth C. Carroll, Shang Jia, Adam Hoagland, Jefferson Chan, Bao Thai, Christine S. Liu, Ehud Y. Isacoff, Christopher J. Chang
NATURE CHEMICAL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Michael H. Berry, Amy Holt, Autoosa Salari, Julia Veit, Meike Visel, Joshua Levitz, Krisha Aghi, Benjamin M. Gaub, Benjamin Sivyer, John G. Flannery, Ehud Y. Isacoff
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Johannes Morstein, Rose Z. Hill, Alexander J. E. Novak, Suihan Feng, Derek D. Norman, Prashant C. Donthamsetti, James A. Frank, Takeshi Harayama, Benjamin M. Williams, Abby L. Parrill, Gabor J. Tigyi, Howard Riezman, Ehud Y. Isacoff, Diana M. Bautista, Dirk Trauner
NATURE CHEMICAL BIOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Prashant C. Donthamsetti, Johannes Broichhagen, Vojtech Vyklicky, Cherise Stanley, Zhu Fu, Meike Visel, Joshua L. Levitz, Jonathan A. Javitch, Dirk Trauner, Ehud Y. Isacoff
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Multidisciplinary Sciences
Chris H. Habrian, Joshua Levitz, Vojtech Vyklicky, Zhu Fu, Adam Hoagland, Isabelle McCort-Tranchepain, Francine Acher, Ehud Y. Isacoff
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Ziwei Li, Qinrong Zhang, Shih-Wei Chou, Zachary Newman, Raphael Turcotte, Ryan Natan, Qionghai Dai, Ehud Y. Isacoff, Na Ji
Article
Biochemistry & Molecular Biology
Andreas Bock, Paolo Annibale, Charlotte Konrad, Annette Hannawacker, Selma E. Anton, Isabella Maiellaro, Ulrike Zabel, Sivaraj Sivaramakrishnan, Martin Falcke, Martin J. Lohse
Review
Physiology
Akiyuki Taruno, Kengo Nomura, Tsukasa Kusakizako, Zhongming Ma, Osamu Nureki, J. Kevin Foskett
Summary: Recent research has made significant progress in understanding the molecular mechanisms behind taste transduction, including the identification of specific taste sensors and components for each taste sensation. The discovery of unconventional chemical synapses and insights into the structures of ion channels have provided new perspectives in the field of taste sensation and neurotransmission.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Horia Vais, Riley Payne, Usha Paudel, Carmen Li, J. Kevin Foskett
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Oncology
Cristina Aguirre-Portoles, Riley Payne, Aspen Trautz, J. Kevin Foskett, Christopher A. Natale, John T. Seykora, Todd W. Ridky
Summary: Testosterone signaling through ZIP9 mediates some of the sex differences in melanoma, and drugs that target AR can be repurposed to block ZIP9 and inhibit melanoma in males.
Article
Biochemistry & Molecular Biology
Selma E. Anton, Charlotte Kayser, Isabella Maiellaro, Katarina Nemec, Jan Moeller, Andreas Koschinski, Manuela Zaccolo, Paolo Annibale, Martin Falcke, Martin J. Lohse, Andreas Bock
Summary: Cells use independent cAMP nanodomains to send different signals, which form self-sufficient cell signaling units. Each nanodomain contains a highly localized cAMP pool that is protected from other receptors and cell compartments. The gradients of local cAMP concentrations define the size of each individual nanodomain. The coexistence of many such nanodomains allows a single cell to operate multiple independent cellular signals simultaneously.
Article
Multidisciplinary Sciences
Katarina Nemec, Hannes Schihada, Gunnar Kleinau, Ulrike Zabel, Eugene O. Grushevskyi, Patrick Scheerer, Martin J. Lohse, Isabella Maiellaro
Summary: This study discovered that receptor-activity-modifying protein 2 (RAMP2) acts as a specific allosteric modulator of parathyroid hormone 1 receptor (PTH1R), shifting it to a preactivated state and affecting its downstream signaling. These findings provide a new avenue for modulating GPCR function and drug design.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Zhongming Ma, Usha Paudel, J. Kevin Foskett
Summary: The intensity of taste is strongly influenced by temperature, but there is a lack of comprehensive understanding about the physiological, hedonic, and commercial implications of this relationship. This study used patch-clamp electrophysiology to investigate the effects of temperature on taste-bud cell electrical excitability, revealing that temperature significantly affects the electrical activity of type II taste-bud cells. These findings provide a mechanism for understanding how temperature influences taste sensitivity and perception.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Review
Physiology
Isabella Maiellaro, Roberto Barbaro, Rosa Caroppo, Matilde Colella
CURRENT OPINION IN PHYSIOLOGY
(2020)