Article
Biochemistry & Molecular Biology
Wei Zhou, Priyapan Posri, Taifo Mahmud
Summary: Nature produces a wide variety of natural compounds through biomanufacturing, sometimes giving rise to hybrid natural products such as the NFAT-133/conglobatin hybrids. Study of the polyketide biosynthetic pathways in Streptomyces pactum ATCC 27456 revealed the structures and biosynthetic mechanisms involved.
ACS CHEMICAL BIOLOGY
(2021)
Article
Immunology
Ruchi Sankhwar, Abhishek Kumar, Shilpi Yadav, Vineeta Singh, Ravi Kr Gupta
Summary: This study aims to isolate, identify and characterize a natural antimicrobial agent against Staphylococcus aureus from natural resources. A potential strain of Streptomyces griseus, RM-1(13), was isolated from Himalayan soils and its active component, Emycin-E, was identified through purification and analysis. Emycin-E showed activity against various Gram-positive and Gram-negative pathogens and inhibited biofilm formation of S. aureus.
MICROBIAL PATHOGENESIS
(2023)
Article
Plant Sciences
Maya Rima, Asma Chbani, Christine Roques, Fatima El Garah
Summary: This study explores the potential antibiofilm activity of extracts derived from three types of algae against Staphylococcus aureus biofilm. Four extracts were found to significantly inhibit biofilm formation, and one extract demonstrated a significant reduction in surface hydrophobicity of S. aureus.
Article
Microbiology
Yunfeng Xu, Weiping Guo, Denglin Luo, Peiyan Li, Jinle Xiang, Junliang Chen, Xiaodong Xia, Qinggang Xie
Summary: This study aimed to investigate the antibiofilm effects of punicalagin against Staphylococcus aureus. The results showed that punicalagin significantly reduced the biomass and metabolic activity of biofilms, decreased the number of viable cells, and damaged the structure of biofilms. The antibiofilm mechanism partly involved the modification of cell surface hydrophobicity. These findings suggest that punicalagin has the potential to be developed as an alternative to control S. aureus biofilms.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Weiping Guo, Yunfeng Xu, Yangyang Yang, Jinle Xiang, Junliang Chen, Denglin Luo, Qinggang Xie
Summary: Oleuropein has the potential to inhibit the formation of Staphylococcus aureus biofilm and reduce bacterial viability.
Article
Biochemistry & Molecular Biology
Yeseul Kim, Sanghun Kim, Kiu-Hyung Cho, Jin-Hyung Lee, Jintae Lee
Summary: Bacterial biofilm formation can be effectively inhibited by cinnamaldehyde and its derivatives, especially 4-nitrocinnamaldehyde. These compounds exhibit strong antibiofilm activities against UPEC and S. aureus, making them promising candidates for the treatment of biofilm-associated diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Caiyun Li, Le Li, Luyao Huang, Aiying Li
Summary: The study reveals that ActVI-ORFA is a pleiotropic regulatory factor that controls multiple physiological pathways, including secondary metabolite production, probably via an indirect mode. The regulatory mechanism of ActVI-ORFA remains unclear, but it is known to positively control the production of actinorhodin in Streptomyces coelicolor.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Sreejita Ghosh, Somdutta Mondol, Dibyajit Lahiri, Moupriya Nag, Tanmay Sarkar, Siddhartha Pati, Soumya Pandit, Abdullah A. Alarfaj, Mohamad Faiz Mohd Amin, Hisham Atan Edinur, Muhammad Rajaei Ahmad Mohd Zain, Rina Rani Ray
Summary: Tinospora cordifolia, a medicinal plant, has been found to have antimicrobial, anti-inflammatory, anti-diabetic, and anti-cancer properties. The silver nanoparticles made from the leaf extract of T. cordifolia were more effective than the phytoextract in reducing the biofilm of Staphylococcus aureus.
FRONTIERS IN CHEMISTRY
(2023)
Article
Infectious Diseases
Jiaman Xu, Quan Lin, Maokun Sheng, Ting Ding, Bing Li, Yan Gao, Yulong Tan
Summary: Food contamination caused by bacteria seriously affects public health. Nanotechnology is being used to combat biofilm formation, and this study successfully prepared cinnamaldehyde-chitosan nanoparticles (CSNP-CAs) which showed higher efficacy in removing Staphylococcus aureus biofilms compared to free drugs. The combination of cinnamaldehyde and CSNPs demonstrated the potential of this composite as an efficient therapeutic agent against biofilm.
Article
Microbiology
Jingyi Yu, Lulin Rao, Lingling Zhan, Yan Zhou, Yinjuan Guo, Xiaocui Wu, Zengqiang Song, Fangyou Yu
Summary: The novel small-molecule ZY-214-4 has the potential to prevent or treat Staphylococcus aureus infections by inhibiting biofilm formation and cell aggregation, without cytotoxicity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Yong-Guy Kim, Jin-Hyung Lee, Sangbum Lee, Young-Kyung Lee, Buyng Su Hwang, Jintae Lee
Summary: The antibiofilm efficacy of six phorbaketals isolated from the Korean marine sponge Phorbas sp. was investigated, showing that phorbaketal B and phorbaketal C effectively inhibit biofilm formation by Staphylococcus aureus strains. Additionally, phorbaketal B also inhibits staphyloxanthin production, which protects microbes from reactive oxygen species, and both compounds inhibit the expression of biofilm-related genes hla and nuc1.
Article
Microbiology
Nilton A. S. Neto, Jose T. A. Oliveira, Tawanny K. B. Aguiar, Leandro P. Bezerra, Levi A. C. Branco, Felipe P. Mesquita, Cleverson D. T. Freitas, Pedro F. N. Souza
Summary: This study investigated the antibiofilm activity of synthetic antimicrobial peptides (SAMPs) in combination with the antibiotic ciprofloxacin against Staphylococcus aureus. The results demonstrated that certain peptides in combination with ciprofloxacin effectively inhibited biofilm formation and degraded preformed biofilms. These active peptides formed pores in the bacterial cellular membrane, enhancing the antibacterial activity of ciprofloxacin.
Article
Biotechnology & Applied Microbiology
Stefana Dukanovi, Tea Ganic, Branka Loncarevic, Stefana Cvetkovic, Biljana Nikolic, Dina Tenji, Danijela Randjelovic, Dragana Mitic-Culafic
Summary: This study demonstrated the novel antibiofilm activity of emodin against Staphylococcus aureus, with strong antibacterial effects and inhibition of biofilm formation. Emodin was also found to affect biofilm structure, respiration, and gene expression, indicating multiple pathways of action against the pathogen.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Yaqian Liu, Pengfei She, Lanlan Xu, Lihua Chen, Yimin Li, Shasha Liu, Zehao Li, Zubair Hussain, Yong Wu
Summary: Penfluridol has shown strong bactericidal activity against Staphylococcus aureus and its clinical isolates, inhibiting biofilm formation and eradicating persister cells. It has demonstrated significant efficacy in mouse infection models and minimal cytotoxicity and hemolysis on human cells, suggesting its potential as a novel antimicrobial agent against bacterial infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
M. Dante Formagio, J. V. de Oliveira Silva, L. Fortunato Prohmann, P. A. Zanetti Campanerut-Sa, I. R. Grenier Capoci, E. Seki Kioshima Cotica, J. M. Graton Mikcha
Summary: This study evaluated the antibacterial and antibiofilm activities of the synthetic compound LMM6 against Staphylococcus aureus. The results showed that LMM6 exhibited significant inhibitory effects on the growth of S. aureus and the formation of biofilms, making it a potential innovative molecule for controlling S. aureus.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Hiroko Kobayashi, Ryo Suzuki, Kei Sato, Takatoshi Ogami, Hiroshi Tomozawa, Masahito Tsubata, Koji Ichinose, Masaki Aburada, Wataru Ochiai, Kiyoshi Sugiyama, Tsutomu Shimada
JOURNAL OF NATURAL MEDICINES
(2018)
Article
Biochemistry & Molecular Biology
Thadee Grocholski, Keith Yamada, Jari Sinkkonen, Heli Tirkkonen, Jarmo Niemi, Mikko Metsa-Ketela
ACS CHEMICAL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Makoto Hashimoto, Takaaki Taguchi, Kazuki Ishikawa, Ryuichiro Mori, Akari Hotta, Susumu Watari, Kazuaki Katakawa, Takuya Kumamoto, Susumu Okamoto, Koji Ichinose
Article
Multidisciplinary Sciences
Riccardo Provenzani, Paola San-Martin-Galindo, Ghada Hassan, Ashenafi Legehar, Aleksi Kallio, Henri Xhaard, Adyary Fallarero, Jari Yli-Kauhaluoma
Summary: Biofilms are challenging in healthcare as they can resist antibiotics, necessitating the development of novel anti-biofilm agents. A study found that some multisubstituted pyrimidine compounds effectively inhibit biofilm formation and kill pre-formed biofilms of Staphylococcus aureus strains. These compounds also showed antibacterial activity against planktonic cells of the staphylococcal strains.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Xiaofeng Cai, Takaaki Taguchi, Huili Wang, Megumi Yuki, Megumi Tanaka, Kai Gong, Jinghua Xu, Yiming Zhao, Koji Ichinose, Aiying Li
Summary: A new C-glycosyltransferase Med-8 is essential for the biosynthesis of medermycin, recognizing a rare deoxyaminosugar. Genes involved in the biosynthesis of angolosamine were proven functional for C-glycosylation. A C-glycosylation cassette composed of these genes could convert a proposed substrate into a C-glycosylated product.
ACS CHEMICAL BIOLOGY
(2021)
Article
Microbiology
Ines Reigada, Paola San-Martin-Galindo, Shella Gilbert-Girard, Jacopo Chiaro, Vincenzo Cerullo, Kirsi Savijoki, Tuula A. Nyman, Adyary Fallarero, Ilkka Miettinen
Summary: When two clinically relevant bacterial pathogens, Staphylococcus aureus and Pseudomonas aeruginosa, co-culture in a biofilm, P. aeruginosa shows higher motility and lower pigment production, while S. aureus exhibits increased persistence due to lower abundances of proteins related to cell wall biosynthesis and cell division. Both species also display higher antimicrobial tolerance when co-cultured. This study provides insights into the dynamics of both the surfaceome and exoproteome of the dual-species biofilms.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xiaofeng Cai, Caiyun Li, Koji Ichinose, Yali Jiang, Ming Liu, Huili Wang, Caixia Gong, Le Li, Juan Wan, Yiming Zhao, Qing Yang, Aiying Li
Summary: Med-ORF10, a single-domain protein of unknown function, plays a regulatory role in medermycin biosynthesis pathway by controlling the expression of genes encoding tailoring enzymes. Its overexpression leads to increased medermycin production and has pleiotropic effects on more targets. However, it is suggested that Med-ORF10 regulates medermycin biosynthesis indirectly, as no direct interaction with target promoter DNA was detected.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Matej Zore, Shella Gilbert-Girard, Ines Reigada, Jayendra Z. Patel, Kirsi Savijoki, Adyary Fallarero, Jari Yli-Kauhaluoma
Summary: By synthesizing 28 novel fingolimod derivatives and evaluating their efficacy against various bacteria, it was found that some derivatives demonstrated improved antibacterial activity against Staphylococcus aureus and other Gram-negative bacteria, with no apparent change in cytotoxicity on human cells. Compound 43 and 55, in particular, showed a broader spectrum of antibacterial activity possibly due to structural modifications.
Article
Biochemistry & Molecular Biology
Takuya Kumamoto, Mika Kainuma, Azusa Takahashi, Yoshika Matsuo, Kazuaki Katakawa, Takaaki Taguchi, Koji Ichinose
Summary: This article reports the total synthesis of 6-deoxydihydrokalafungin (DDHK) and its epimer, epi-DDHK, as well as a semisynthetic approach from (S)-DNPA. Different reaction sequences were used to successfully obtain these two compounds.
Article
Materials Science, Multidisciplinary
Paola San-Martin-Galindo, Emil Rosqvist, Stiina Tolvanen, Ilkka Miettinen, Kirsi Savijoki, Tuula A. Nyman, Adyary Fallarero, Jouko Peltonen
Summary: Investigating microbial response to different surfaces requires versatile parameters, including surface chemistry and topography. Surface roughness parameters influence microbial viability and exopolysaccharide abundance, with valley structures potentially increasing viability.
MATERIALS & DESIGN
(2021)
Article
Biochemistry & Molecular Biology
Kazuki Ishikawa, Masaki Ishii, Takashi Yaguchi, Toshiaki Katada, Koji Ichinose, Shinya Ohata
Summary: The nuclear factor-kappa B (NF-kappa B) signaling pathway plays a crucial role in inflammatory responses and cancer pathogenesis. In this study, the alkaloid aszonalenin and its analog epi-aszonalenin B were identified as inhibitors of NF-kappa B activity, making them potential therapeutic compounds for supratentorial ependymomas (ST-EPN-RELA).
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
Kazuki Ishikawa, Makoto Hashimoto, Kunpei Komatsu, Takaaki Taguchi, Susumu Okamoto, Koji Ichinose
Summary: Actinorhodin (ACT) is an antibiotic produced by Streptomyces coelicolor A3(2), which has been widely studied as a model organism for antibiotic biosynthesis and regulation. The study found that ActVI-ORF2 is a stereospecific enoyl reductase that converts (S)-DNPA to DDHK.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Microbiology
Matej Zore, Shella Gilbert-Girard, Paola San-Martin-Galindo, Ines Reigada, Leena Hanski, Kirsi Savijoki, Adyary Fallarero, Jari Yli-Kauhaluoma, Jayendra Z. Patel
Summary: This study reveals the potential of etrasimod as a repurposed antibacterial compound against Gram-positive bacteria, displaying inhibitory activity and synergistic effect with gentamicin, while also showing potential to inhibit biofilm formation in certain Gram-negative bacteria.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Makoto Hashimoto, Susumu Watari, Takaaki Taguchi, Kazuki Ishikawa, Takuya Kumamoto, Susumu Okamoto, Koji Ichinose
Summary: In this study, we identified two enzymes, ActVA-ORF4 and ActVA-ORF3, involved in the last step of ACT biosynthesis. ActVA-ORF4 catalyzes the intermolecular C-C bond formation using DHK-OH as the substrate, while ActVA-ORF3 is a quinone-forming enzyme that produces the coupling substrate DHK-OH and the final product ACT.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Food Science & Technology
Kirsi Savijoki, Paola San-Martin-Galindo, Katriina Pitkaenen, Minnamari Edelmann, Annika Sillanpaeae, Cim van der Velde, Ilkka Miettinen, Jayendra Z. Patel, Jari Yli-Kauhaluoma, Mataleena Parikka, Adyary Fallarero, Pekka Varmanen
Summary: By studying the effects of metabolic end-products of food-associated lactobacilli and propionibacteria on biofilm formation of Gram-negative pathogenic bacteria, it was found that these end-products can disrupt the quorum sensing system of the pathogenic bacteria, leading to the inhibition of biofilm formation.