Article
Agriculture, Multidisciplinary
Min Jeong Kim, Chang Ki Shim, Jong-Ho Park
Summary: This study focused on the biological control of late blight in eco-friendly potato cultivation in Korea. The AFB2-2 strain showed the ability to inhibit the growth of plant pathogens and demonstrated high control efficacy in experiments. The genetic sequences of AFB2-2 strain were found to be highly similar to other Bacillus species.
PLANT PATHOLOGY JOURNAL
(2021)
Article
Plant Sciences
Nisha Rokaya, Anita Paneru, Ram Devi Timila, Shambhu Prasad Dhital, Ram Kumar Shrestha, Gopal Bahadur Kc, Hira Kaji Manandhar
Summary: This study evaluated and screened 39 isolates of Trichoderma spp. for their efficacy against potato late blight. Among them, 15 isolates significantly reduced the lesion size of late blight. The TL1-2A isolate was identified as a potential candidate for the integrated management of potato late blight in Nepal, as it significantly reduced disease severity and increased plant growth and tuber yield in field experiments.
JOURNAL OF PHYTOPATHOLOGY
(2023)
Article
Plant Sciences
Ki-Beom Moon, Su-Jin Park, Ji-Sun Park, Hyo-Jun Lee, Seung Young Shin, Soo Min Lee, Gyung Ja Choi, Sang-Gyu Kim, Hye Sun Cho, Jae-Heung Jeon, Yong-Sam Kim, Youn-Il Park, Hyun-Soon Kim
Summary: This study established an efficient CRISPR/Cas9 genome editing protocol for potato, targeting the susceptibility gene SR4 to develop Phytophthora infestans resistant mutants. The co-expression of StEDS1 and StPAD4 induced the accumulation of salicylic acid (SA) in the mutants, enhancing their resistance to the pathogen. However, high SA content inhibited the growth of mutants and induced a dwarf phenotype.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yongfei Jian, Shun Feng, Airong Huang, Zhiming Zhu, Jiaomei Zhang, Shicai Tang, Liang Jin, Maozhi Ren, Pan Dong
Summary: In this study, it was found that Diallyl trisulfide (DATS) can inhibit the growth of Phytophthora infestans and reduce its pathogenicity on potato leaves and tubers. Moreover, DATS can induce overall resistance of potato tubers by increasing catalase (CAT) activity. Transcriptome analysis revealed differentially expressed genes (DEGs) and miRNAs (DEMs) in response to DATS treatment, with several metabolic pathways being affected. These findings provide new insights into the biocontrol of potato late blight using DATS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Amira A. Al Harethi, Qais Y. M. Abdullah, Hala J. Al Jobory, Samar A. Al Aquil, Ramadan A. Arafa
Summary: This study successfully isolated and identified Phytophthora infestans in Yemen using advanced methods, providing fundamental data for the prevention and control of late blight.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Jingyu Zhu, Xue Tang, Yining Sun, Yan Li, Yajie Wang, Yusong Jiang, Huanhuan Shao, Bin Yong, Honghao Li, Xiang Tao
Summary: This study used untargeted metabolomics to investigate the changes in metabolites during the infection process of Phytophthora infestans in potato cultivars. The results showed that metabolic differences mainly emerged after 48 hours postinoculation. Comparisons between the resistant and susceptible cultivars identified metabolites related to resistance, including higher levels of salicylic acid in the resistant cultivar. These metabolites play crucial roles in cell wall thickening and have antibacterial and antifungal activities. This study reveals the metabolomic responses of potatoes to P. infestans and provides insights into the compatible and incompatible interactions between potatoes and P. infestans.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Xinyi Hu, Kristian Persson Hoden, Zhen Liao, Anna Asman, Christina Dixelius
Summary: Phytophthora spp. cause damage to plants by using effector proteins and small RNAs. The study found that the sRNA encoded by P. infestans can affect potato mRNA, expanding our understanding of the strategies used by this species to facilitate infection.
Article
Biochemistry & Molecular Biology
Zhihua Wu, Haichen Cui, Zhining Sun, Huirong Liu
Summary: The study identified Myxococcus xanthus strain B25-I-1 with strong antagonistic activity against Phytophthora infestans, indicating its potential as a biological control agent against potato late blight. The active extract produced by B25-I-1 showed inhibitory effects on the growth and reproductive structures of P. infestans, making it a promising candidate for the development of biological pesticides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Agronomy
Amanda C. Saville, Federico La Spada, Roberto Faedda, Quirico Migheli, Bruno Scanu, Paolo Ermacora, Giovanna Gilardi, Giorgia Fedele, Vitorrio Rossi, Nicolo Lenzi, Antonino Testa, Mohamed Bechir Allagui, Marwa Moumni, Enza Dongiovanni, Fatma Zohra Rekad, David E. L. Cooke, Antonella Pane, Santa O. Cacciola, Jean B. Ristaino
Summary: This genetic study of Phytophthora infestans samples collected from Italy, Algeria, and Tunisia identified four clonal lineages and highlighted potential new sources of introduction into Italy.
Article
Biochemistry & Molecular Biology
Xiaoqing Huang, Ziyue You, Yang Luo, Chengji Yang, Jie Ren, Yanlin Liu, Guangjing Wei, Pan Dong, Maozhi Ren
Summary: Chitosan shows significant inhibition on the mycelial growth and spore germination of Phytophthora infestans, reducing its resistance to adverse conditions and having synergistic effects with pesticides. It also induces resistance in potatoes to the pathogen, with transcriptome analysis showing effects on cell growth and key pathways. This study provides new insights for the development of chitosan as an eco-friendly solution for controlling potato late blight.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Kangkai Zheng, Jie Lu, Jie Li, Yin Yu, Jia Zhang, Ziwei He, Omayma M Ismail, Jie Wu, Xuejun Xie, Xiaobo Li, Guangyuan Xu, Daolong Dou, Xiaodan Wang
Summary: The study demonstrated that chitosan can enhance potato resistance and reduce Phytophthora infestans infection by activating important plant defence response pathways. In addition, chitosan triggers pattern recognition immune responses, induces systemic resistance, and alters gene expression related to plant defence in potato. These findings provide insights into chitosan-mediated activation of the immune system in potato and offer a potential eco-friendly strategy for controlling late blight in the field.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Genevieve Leger, Amy Novinscak, Adrien Biessy, Simon Lamarre, Martin Filion
Summary: Different PCA-producing Pseudomonas spp. strains exhibited varying levels of biocontrol activity against Phytophthora infestans in potato tuber bioassays. The quantities of PCA produced did not correlate with biocontrol activity, suggesting the presence of other genetic determinants potentially involved in controlling the pathogen. Genome mining identified siderophores, cyclic lipopeptides, and non-ribosomal peptide synthase and polyketide synthase hybrid clusters as potential factors in the biocontrol of Phytophthora infestans by phenazine-producing pseudomonads.
Article
Biotechnology & Applied Microbiology
Liang Jin, Shun Feng, Shicai Tang, Pan Dong, Zhengguo Li
Summary: This study used Streptomyces strains and biochar for the biological control of Potato Late Blight (PLB). In vitro experiments demonstrated that the six Streptomyces strains could effectively inhibit the growth and sporangia germination of Phytophthora infestans. In vivo tests showed that the six Streptomyces strains significantly reduced the infection of P. infestans on potato tubers. Combined with biochar, the inhibition rate of Streptomyces strains against P. infestans increased by 1.9% to 46.1%. Field trials conducted over two years demonstrated that the combination of Streptomyces strains and biochar reduced the disease index by 10% to 26%, exhibiting superior disease control compared to using either agent alone. Additionally, a single application of biochar did not significantly decrease disease incidence but enriched the rhizosphere with beneficial bacterium Rhizobium. The results highlight the potential of biological control of PLB using a combination of Streptomyces strains and biochar.
BIOLOGICAL CONTROL
(2023)
Article
Plant Sciences
Chenchen Wang, Hongjuan Gao, Zhaohui Chu, Changquan Ji, Yang Xu, Weilin Cao, Shumei Zhou, Yunzhi Song, Hongmei Liu, Changxiang Zhu
Summary: The study identified StLTP10 as a positive regulator of resistance to Phytophthora infestans in potato by enhancing the expression of defense-related genes. StLTP10 was found to physically interact with an ABA receptor and a wound-induced protein kinase, influencing stomatal closure and protein abundance, respectively. These findings provide insight into the molecular mechanisms underlying plant immune responses and suggest potential targets for enhancing broad-spectrum resistance in potato.
MOLECULAR PLANT PATHOLOGY
(2021)
Review
Agriculture, Multidisciplinary
Suo-meng Dong, Shao-qun Zhou
Summary: This article summarizes the molecular pathogenesis of potato late blight and current control strategies, emphasizing the importance of continuous improvement in efficient and sustainable management.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)