Article
Biochemistry & Molecular Biology
Anella Saggese, Rosa Giglio, Nicola D'Anzi, Loredana Baccigalupi, Ezio Ricca
Summary: Spore formers are ubiquitous microorganisms that can form spores in the gastrointestinal tract of animals, and they have beneficial effects on the gut, such as antimicrobial and antibiofilm activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Laura M. Brutscher, Claudia Borgmeier, Sean M. Garvey, Jessica L. Spears
Summary: In this study, the safety of Bacillus subtilis BS50 was assessed through preclinical analyses. The results showed that BS50 genome does not encode any known toxins, is susceptible to commonly prescribed antibiotics, and does not have any negative effects on the viability and permeability of human intestinal epithelial cells. Therefore, BS50 has a preclinical safety profile similar to commercial probiotic strains and poses little risk to human health.
Article
Nutrition & Dietetics
Jessica L. Spears, Richard Kramer, Andrey Nikiforov, Marisa O. Rihner, Elizabeth A. Lambert
Summary: The safety and tolerability of the unique strain Bacillus subtilis MB40 was assessed through in silico, in vitro, and in vivo studies. The study confirmed the absence of undesirable genetic elements encoding toxins and antibiotic resistance, and found that MB40 was well tolerated in both rats and healthy human volunteers. Based on the research, it was concluded that Bacillus subtilis MB40 does not pose any human health concerns under the tested conditions.
Article
Microbiology
Maria Vittoria, Anella Saggese, Rachele Isticato, Loredana Baccigalupi, Ezio Ricca
Summary: Thirty-two aerobic spore former strains isolated from healthy children's intestinal samples were analyzed for hemolytic and antibiotic-resistant activities. Four selected non-hemolytic strains, sensitive to all relevant antibiotics, were evaluated for their probiotic potentials in silico and in vitro. Genomic and physiological analysis showed that these strains do not pose safety concerns and have the potential to be used as an alternative to antibiotics.
Article
Biochemistry & Molecular Biology
Michael A. DiCandia, Adrianne N. Edwards, Joshua B. Jones, Grace L. Swaim, Brooke D. Mills, Shonna M. McBride
Summary: The study reveals that Spo0A in Clostridioides difficile interacts with similar proteins as in Bacillus, despite the absence of corresponding proteins in the C. difficile genome. Mutation analysis shows that several conserved residues in Spo0A affect the sporulation ability of C. difficile. Furthermore, the phosphorylation site of Spo0A and its impact on activity are determined.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Microbiology
Anella Saggese, Giovanni Di Gregorio Barletta, Maria Vittoria, Giuliana Donadio, Rachele Isticato, Loredana Baccigalupi, Ezio Ricca
Summary: The CotG protein in the Bacillus subtilis spore coat controls spore permeability through its central region of positively charged tandem repeats. The presence of central repeats is a common feature in CotG-like proteins in most Bacillus species. This study contributes to the understanding of the structure and function of bacterial spores.
Review
Microbiology
Emilia Ghelardi, Ana Teresa Abreu y Abreu, Christian Boggio Marzet, Guillermo Alvarez Calatayud, Marcos Perez, Ana Paula Moschione Castro
Summary: Bacillus clausii is a probiotic with good tolerance and beneficial properties for human health. Although widely used, its advantages are not fully disseminated. Some properties of Bacillus clausii may bring more health benefits in other therapeutic areas.
Article
Biotechnology & Applied Microbiology
Jiajia Li, Jie Yang, Weishan Xin, Sirui Wu, Xujuan Wang, Chuanfa Wang, Zhong Zhang
Summary: Combining High-pressure Thermal Treatment (HPTT) and Potassium Sorbate (PS) has a stronger spore inactivation effect. The combination treatment resulted in a higher inactivation rate of Bacillus subtilis spores compared to HPTT treatment alone. It also caused changes in spore permeability, size, inner membrane damage, and reduced enzyme activity. Consequently, the combination of HPTT and PS is proposed as a promising strategy for Bacillus subtilis spore inactivation.
Article
Microbiology
Su-Hyeon Kim, Gashaw Assefa Yehuala, Won Yeong Bang, Jungwoo Yang, Young Hoon Jung, Mi-Kyung Park
Summary: The present study evaluated the safety of Bacillus subtilis (BS) IDCC1101, a newly isolated strain from Cheonggukjang in Korea. Genome sequencing revealed the presence of secondary metabolites, but antibiotic resistance and virulence genes were not transferable. BS IDCC1101 showed susceptibility to most antibiotics, utilized a wide range of carbohydrates, and exhibited various enzyme activities. It did not produce lactate or show hemolytic activity. Furthermore, no toxicity was observed in HaCaT cells and rats. These findings suggest that BS IDCC1101 can be safely used for industrial applications.
Article
Veterinary Sciences
Aoyun Li, Meng Wang, Yu Zhang, Zhengrong Lin, Mengen Xu, Lei Wang, Muhammad Fakhar-e-Alam Kulyar, Jiakui Li
Summary: This study investigated the genome and genetic properties of Bacillus subtilis derived from yaks, contributing to understanding the potential prebiotic mechanism of probiotics from a genetic perspective.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Fisheries
Sukanta K. Nayak
Summary: Microorganisms, particularly probiotics like Bacillus species, play a vital role in reducing the use of antibiotics and drugs in aquaculture. They improve growth, nutrition, immunity, and disease resistance in aquatic species, making them a valuable tool for sustainable and safe aquaculture practices.
REVIEWS IN AQUACULTURE
(2021)
Article
Microbiology
Ida Szmigiel, Damian Konkol, Mariusz Korczynski, Marcin Lukaszewicz, Anna Krasowska
Summary: This study found that the addition of 3% fermented rapeseed meal led to beneficial histological changes in the ceca of broiler chickens, inhibited the growth of pathogens, and stimulated the growth of probiotic bacteria.
Article
Biochemistry & Molecular Biology
A. Vetrakova, R. Kaliankova Chovanova, R. Rechtorikova, D. Krajcikova, I. Barak
Summary: In this study, recombinant spores displaying the receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein on their surface were prepared as a possible tool for developing an oral vaccine against the SARS-CoV-2 virus. The RBD was attached to the spore coat proteins and its surface expression was confirmed using SDS-PAGE, western blot, and fluorescence microscopy.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Rachele Isticato
Summary: Mucosal vaccines have advantages such as inducing adaptive immunity and needle-less vaccinations, but their development is currently limited. Innovative antigens, efficient adjuvants, and delivery systems are crucial for effective mucosal vaccines. Bacterial spores have shown promise as antigen vehicles due to their unique structure and ability to induce protective immune responses when mucosally administered.
Article
Biotechnology & Applied Microbiology
Ke Bi, Yue Liu, Weishan Xin, Jie Yang, Bianfei Zhang, Zhong Zhang
Summary: The combined heat and e-polylysine treatments effectively inactivated Bacillus subtilis spores, causing damage to cell membranes and increased permeability. Microscopy analysis revealed significant changes in spore morphology, while Fourier transform infrared spectra indicated alterations in the inner membrane and changes in the structure of peptidoglycan layer, as well as protein and nucleic acids denaturation.
Article
Biochemistry & Molecular Biology
Imen Ben Slimene, Olfa Tabbene, Dorra Gharbi, Bacem Mnasri, Jean Marie Schmitter, Maria-Camino Urdaci, Ferid Limam
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2015)
Article
Microbiology
Rebeca Martin, Borja Sanchez, Maria C. Urdaci, Philippe Langella, Juan E. Suarez, Luis G. Bermudez-Humaran
Article
Geriatrics & Gerontology
Marie Lefevre, Silvia M. Racedo, Gabrielle Ripert, Beatrice Housez, Murielle Cazaubiel, Corinne Maudet, Peter Juesten, Philippe Marteau, Maria C. Urdaci
Article
Immunology
P. Carasi, S. M. Racedo, C. Jacquot, D. E. Romanin, M. A. Serradell, M. C. Urdaci
JOURNAL OF IMMUNOLOGY RESEARCH
(2015)
Review
Endocrinology & Metabolism
Melanie Le Barz, Fernando F. Anhe, Thibaut V. Varin, Yves Desjardins, Emile Levy, Denis Roy, Maria C. Urdaci, Andre Marette
DIABETES & METABOLISM JOURNAL
(2015)
Article
Microbiology
Gabrielle Ripert, Silvia M. Racedo, Anne-Marie Elie, Claudine Jacquot, Philippe Bressollier, Maria C. Urdaci
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2016)
Article
Chemistry, Physical
Alexandre Cunha, Anne-Marie Elie, Laurent Plawinski, Ana Paula Serro, Ana Maria Botelho do Rego, Amelia Almeida, Maria C. Urdaci, Marie-Christine Durrieu, Rui Vilar
APPLIED SURFACE SCIENCE
(2016)
Article
Medicine, Legal
Marie Lefevre, Silvia M. Racedo, Muriel Denayrolles, Gabrielle Ripert, Thomas Desfougeres, Alexandra R. Lobach, Ryan Simon, Fanny Pelerin, Peter Justen, Maria C. Urdaci
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2017)
Article
Immunology
Paula Carasi, Silvia Maria Racedo, Claudine Jacquot, Anne Marie Elie, Maria de los Angeles Serradell, Maria C. Urdaci
FRONTIERS IN IMMUNOLOGY
(2017)
Article
Cell Biology
Gabriela Uribe, Romain Villeger, Philippe Bressollier, Rachel N. Dillard, Daniel L. Worthley, Timothy C. Wang, Don W. Powell, Maria C. Urdaci, Irina V. Pinchuk
CELLULAR MICROBIOLOGY
(2018)
Article
Microbiology
Maria C. Urdaci, Marie Lefevre, Guylene Lafforgue, Christel Cartier, Bertrand Rodriguez, Jean Fioramonti
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Food Science & Technology
Paula Carasi, Claudine Jacquot, David E. Romanin, Anne-Marie Elie, Graciela L. De Antoni, Maria C. Urdaci, Maria de los Angeles Serradell
INTERNATIONAL DAIRY JOURNAL
(2014)
Article
Agriculture, Dairy & Animal Science
Paula Carasi, Nicolas M. Ambrosis, Graciela L. De Antoni, Philippe Bressollier, Maria C. Urdaci, Maria de los Angeles Serradell
JOURNAL OF DAIRY RESEARCH
(2014)
Article
Biochemistry & Molecular Biology
Melanie Le Barz, Noemie Daniel, Thibault V. Varin, Sabrine Naimi, Veronique Demers-Mathieu, Genevieve Pilon, Julie Audy, Emilie Laurin, Denis Roy, Maria C. Urdaci, Daniel St-Gelais, Ismail Fliss, Andre Marette
Article
Zoology
Maria C. Urdaci, Catherine Taverny, Anne-Marie Elie, Pierre Elie