Article
Gastroenterology & Hepatology
Luz del Carmen Martinez-Sanchez, Phuong Anh Ngo, Rashmita Pradhan, Lukas-Sebastian Becker, David Boehringer, Despina Soteriou, Marketa Kubankova, Christine Schweitzer, Tatyana Koch, Veronika Thonn, Lena Erkert, Iris Stolzer, Claudia Guenther, Christoph Becker, Benno Weigmann, Monika Klewer, Christoph Daniel, Kerstin Amann, Stefan Tenzer, Raja Atreya, Martin Bergo, Cord Brakebusch, Alastair J. M. Watson, Jochen Guck, Ben Fabry, Imke Atreya, Markus F. Neurath, Rocio Lopez-Posadas
Summary: Abnormal cell shedding is associated with intestinal barrier dysfunction and inflammatory bowel diseases. Deletion of PGGTase and RAC1 in intestinal epithelial cells leads to cell overcrowding and epithelial leakage, resulting in chronic intestinal inflammation. RAC1 plays a crucial role in regulating cytoskeletal dynamics, cell mechanics, and intestinal cell shedding.
Review
Biochemistry & Molecular Biology
Rina Fujiwara-Tani, Shiori Mori, Ruiko Ogata, Rika Sasaki, Ayaka Ikemoto, Shingo Kishi, Masuo Kondoh, Hiroki Kuniyasu
Summary: CLDN4 is a crucial component of tight junctions in epithelial cells and is overexpressed in many epithelial malignancies. Changes in CLDN4 expression are associated with epigenetic factors, inflammation, and growth factor signaling. CLDN4 plays a role in maintaining the tumor microenvironment and acts as a barrier to anticancer drug entry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Lucas Heils, Martina Schneemann, Ralf Gerhard, Joerg-Dieter Schulzke, Roland Buecker
Summary: The study investigated the impact of Clostridioides difficile binary toxin (CDT) on the intestinal epithelial barrier and found that sublethal concentrations of CDT induced barrier dysfunction with increased permeability and redistribution of tight junction proteins. This suggests that CDT may contribute to diarrhea by compromising the intestinal barrier function.
Article
Food Science & Technology
Yunika Mayangsari, Mayu Okudaira, Chinatsu Mano, Yuki Tanaka, Osamu Ueda, Tomohiro Sakuta, Yoshiharu Suzuki, Yoshinari Yamamoto, Takuya Suzuki
Summary: Research showed that DMF can enhance intestinal TJ barrier integrity by increasing the expression of occludin and claudin-1, while reducing the expression of claudin-2. This regulation involves the induction of occludin protein translation via mTOR and silencing Cldn2 mRNA via miR-16-5p.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Biochemistry & Molecular Biology
Pradeep K. Shukla, Avtar S. Meena, Joseph F. Pierre, RadhaKrishna Rao
Summary: This study reveals that the glucocorticoid receptor (GR) plays a crucial role in alcohol-induced gut barrier dysfunction and systemic response. The absence of GR in intestinal epithelial cells leads to resistance to alcohol-induced symptoms such as microbiota dysbiosis, endotoxemia, and systemic inflammation, while the absence of GR in hepatocytes results in protection against liver damage. This study identifies the intestinal epithelial GR as a potential therapeutic target for alcohol-associated tissue injury.
Review
Gastroenterology & Hepatology
Clara Abraham, Maria T. Abreu, Jerrold R. Turner
Summary: This review focuses on the role of pattern recognition receptors in the inflammatory response and regulation of epithelial barrier in inflammatory bowel disease. It explores the cytokine networks that contribute to inflammation, regulation of paracellular permeability, epithelial damage, up-regulation of epithelial proliferation, and restitutive processes. Patient samples are used in studies and potential pathways for a more comprehensive treatment approach for patients with inflammatory bowel disease are speculated.
Article
Cell Biology
Sandra Chanez-Paredes, Armando Montoya-Garcia, Karla F. Castro-Ochoa, Julio Garcia-Cordero, Leticia Cedillo-Barron, Mineko Shibayama, Porfirio Nava, Sven Flemming, Nicolas Schlegel, Alexis M. Gautreau, Hilda Vargas-Robles, Ricardo Mondragon-Flores, Michael Schnoor
Summary: The Arp2/3 inhibitory protein arpin plays a crucial role in maintaining the integrity of the intestinal epithelial barrier (IEB) and its expression is regulated by inflammatory factors. Lower levels of arpin can trigger dysfunction of IEB during inflammation. Treatment with the Arp2/3-specific inhibitor CK666 can alleviate inflammation-induced permeability barriers, suggesting a potential therapeutic strategy for IEB-related disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, General & Internal
Chun-Yan Yeung, Jen-Shiu Chiang Chiau, Mei-Lein Cheng, Wai-Tao Chan, Chuen-Bin Jiang, Szu-Wen Chang, Chia-Yuan Liu, Ching-Wei Chang, Hung-Chang Lee
Summary: In this study, an animal model was established by feeding mice a VD-deficient diet to investigate its effects on gut integrity and zonulin expression. The results showed that VD deficiency could lead to impaired barrier properties, suggesting that sufficient VD may help maintain intestinal epithelial integrity and prevent mucosal barrier dysfunction.
FRONTIERS IN MEDICINE
(2021)
Article
Microbiology
Xiaodan Shi, Wei Xu, Xinghua Che, Jiawen Cui, Xinchi Shang, Xiaohua Teng, Zhiying Jia
Summary: The research found that aquatic organisms such as fish can accumulate high concentrations of arsenic (As), which has toxic effects on fish. However, whether the intestinal flora are involved in As damage to fish intestinal tissues and the underlying process are unclear.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Shen Zhuang, Ke Ming, Ning Ma, Jinrui Sun, Dehai Wang, Mingxing Ding, Yi Ding
Summary: A specific polysaccharide fraction (POPZ) from Portulaca oleracea L. was found to inhibit LPS-induced barrier injury and inflammatory responses in porcine intestinal epithelial monolayer, possibly through inactivating TLR4/NF-κB pathway and activating EGF/EGFR pathway.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Nanoscience & Nanotechnology
Kun Man, Jiafeng Liu, Khang Minh Phan, Kai Wang, Jung Yeon Lee, Xiankai Sun, Michael Story, Debabrata Saha, Jun Liao, Hamid Sadat, Yong Yang
Summary: Mechanical stretch is critical for the function and homeostasis of cells and tissues. However, the effects of stretch dimensionality on cell behavior are often overlooked. This study develops cell culture platforms that mimic different types of mechanical stretches and shows that stretch dimensionality influences cell morphology and interactions.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Ya-Nan Gao, Song-Li Li, Xue Yang, Jia-Qi Wang, Nan Zheng
Summary: The study found that lactoferrin (LF) can reduce the increased intestinal permeability caused by aflatoxin M1 (AFM1) and repair the damaged intestinal barrier. In addition, the research revealed that LF acts through regulating multiple pathways related to intestinal cell survival and intestinal integrity. These findings suggest that LF may be a potential option for treating compromised intestinal barrier.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Camila Daza-Leon, Arlen P. Gomez, Diana Alvarez-Mira, Loren Carvajal-Diaz, Gloria Ramirez-Nieto, Andres Sanchez, Jose I. Vargas, Liliana Betancourt
Summary: The objective of this study was to evaluate the effect of propolis supplementation in the diet of broilers. The results showed that the inclusion of Colombian propolis can improve productive performance, physiological parameters, and gene expression associated with intestinal integrity in broilers.
Article
Biochemistry & Molecular Biology
Mio Nakashima, Natsuko Goda, Takeshi Tenno, Ayaka Kotake, Yuko Inotsume, Minako Amaya, Hidekazu Hiroaki
Summary: Modulation of tight junction integrity by small molecules is important for drug delivery. Baicalin, baicalein, quercetin, and hesperetin have been shown to open tight junctions in MDCK II cells, but the mechanisms for hesperetin and quercetin are still unclear. This study compared the effects of hesperetin and quercetin on cell proliferation, morphological changes, and tight junction integrity. Hesperetin and quercetin had opposite effects on cell viability, with hesperetin promoting proliferation and quercetin suppressing it. Only quercetin induced a change in cell shape. Both hesperetin and quercetin downregulated the subcellular localization of claudin-2, but only quercetin downregulated its expression. Hesperetin was shown to directly bind to the first PDZ domain of ZO-1, while quercetin did not. The TGF beta pathway partially contributed to hesperetin-induced cell proliferation, while the MEK pathway was not involved in the effects of either flavonoid. These findings provide insight into the use of hesperetin or quercetin as naturally occurring absorption enhancers via the paracellular route.
Article
Ophthalmology
Aihua Hou, Safiah Mohamed Ali, Evelyn Png, Walter Hunziker, Louis Tong
Summary: The purpose of this study was to investigate the role of TG-2 in corneal barrier function and its regulation of epithelial junctional proteins and transcription factors. Corneas from TG-2-/- mice showed lower trans-epithelial electrical resistance and increased central corneal thickness compared to TG-2+/+ mice. TG-2-/- mice also exhibited a faster increase in fluorescence in the anterior chamber after ocular exposure to FITC-dextran. The expression of Claudin-1 protein and transcript levels were reduced in the cornea of TG-2-/- mice and in TG-2 knockdown human corneal epithelial cells.
Article
Medicine, Research & Experimental
Joanne C. Masterson, Kathryn A. Biette, Juliet A. Hammer, Nathalie Nguyen, Kelley E. Capocelli, Bejan J. Saeedi, Rachel F. Harris, Shahan D. Fernando, Lindsay B. Hosford, Caleb J. Kelly, Eric L. Campbell, Stefan F. Ehrentraut, Faria N. Ahmed, Hiroshi Nakagawa, James J. Lee, Eoin N. McNamee, Louise E. Glover, Sean P. Colgan, Glenn T. Furuta
JOURNAL OF CLINICAL INVESTIGATION
(2019)
Article
Pathology
Brian S. Robinson, Bejan Saeedi, Connie M. Arthur, Josh Owens, Crystal Naudin, Nourine Ahmed, Liping Luo, Rheinallt Jones, Andrew Neish, Sean R. Stowell
AMERICAN JOURNAL OF PATHOLOGY
(2020)
Article
Cell Biology
Bejan J. Saeedi, Ken H. Liu, Joshua A. Owens, Sarah Hunter-Chang, Mary C. Camacho, Richard U. Eboka, Bindu Chandrasekharan, Nusaiba F. Baker, Trevor M. Darby, Brian S. Robinson, Rheinallt M. Jones, Dean P. Jones, Andrew S. Neish
Article
Multidisciplinary Sciences
Ruth X. Wang, J. Scott Lee, Eric L. Campbell, Sean P. Colgan
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Obstetrics & Gynecology
D. A. Crosby, L. E. Glover, E. P. Brennan, P. Kelly, P. Cormican, B. Moran, F. Giangrazi, P. Downey, E. E. Mooney, B. J. Loftus, F. M. McAuliffe, M. Wingfield, C. O'Farrelly, D. J. Brennan
HUMAN REPRODUCTION
(2020)
Article
Cell Biology
Alexander S. Dowdell, Ian M. Cartwright, Matthew S. Goldberg, Rachael Kostelecky, Tyler Ross, Nichole Welch, Louis E. Glover, Sean P. Colgan
MOLECULAR BIOLOGY OF THE CELL
(2020)
Article
Medicine, General & Internal
David A. Crosby, Louise E. Glover, Paul Downey, Eoghan E. Mooney, Fionnuala M. McAuliffe, Cliona O'Farrelly, Donal J. Brennan, Mary Wingfield
Summary: In this study, there were no differences in implantation rates, clinical pregnancy rates, or live birth rates between nulliparous women who underwent a accurately timed mid-luteal scratch biopsy prior to ART and those who did not undergo the procedure.
IRISH JOURNAL OF MEDICAL SCIENCE
(2021)
Article
Obstetrics & Gynecology
David A. Crosby, Louise E. Glover, Paul Downey, Eoghan E. Mooney, Fionnuala M. McAuliffe, Cliona O'Farrelly, Donal J. Brennan, Mary Wingfield
Summary: This study aimed to predict pregnancy outcomes in assisted reproduction treatment by evaluating endometrial thickness, morphology, and uterine artery Doppler parameters. Results showed that ultrasound assessment of the endometrium did not differentiate between patients who would have a subsequent clinical pregnancy.
Review
Multidisciplinary Sciences
Zsuzsanna Kovacs, Louise Glover, Fiona Reidy, John MacSharry, Radka Saldova
Summary: This review aims to summarize minimally invasive or non-invasive diagnostic methods for endometriosis and discuss the potential of novel disease biomarkers of microbial or glycan origin. Efforts have been made to develop accurate diagnostic methods, considering associations between microbial health, hormonal imbalance, and endometriosis. Glycomics could provide valuable biomarkers for innovative diagnostics in this debilitating disease.
JOURNAL OF ADVANCED RESEARCH
(2021)
Review
Nutrition & Dietetics
Theo Wallimann, Caroline H. T. Hall, Sean P. Colgan, Louise E. Glover
Summary: Oral supplementation of creatine monohydrate may be effective in reducing or alleviating symptoms in patients with inflammatory bowel diseases, and current pilot clinical trials will provide valuable insights for potential larger studies in the future.
Article
Biochemistry & Molecular Biology
Brittany R. Jenkins, Nathan A. Blaseg, Heather M. Grifka-Walk, Benjamin Deuling, Steve D. Swain, Eric L. Campbell, Seth T. Walk, Douglas J. Kominsky
Summary: IL-10 signaling plays a crucial role in regulating intestinal mucosal homeostasis by modulating the balance of proliferation and differentiation in IECs. Depletion of IL-10 signaling leads to a shift towards goblet cell differentiation, increased proliferation, and minor changes in apoptosis. Further research is needed to explore the detailed mechanisms underlying these cellular processes.
Review
Obstetrics & Gynecology
Maebh Horan, Louise Glover, Mary Wingfield
Summary: The management of endometriomas in women wishing to preserve their fertility is complex, with surgery potentially impacting ovarian reserve. Assisted reproduction and oocyte cryopreservation play important roles in the evolving treatment of this disease. It is crucial to consider future fertility when navigating various management strategies for endometriomas.
INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS
(2022)
Editorial Material
Multidisciplinary Sciences
Agnieszka A. Kendrick, Jenna R. Christensen
Summary: Recent research revealed that ARL8 acts as an adaptor protein, connecting lysosomes to both kinesin for anterograde movement and dynein for retrograde movement, providing crucial insights into the regulation of bidirectional organelle movement in cells.
NATURE COMMUNICATIONS
(2022)
Review
Nutrition & Dietetics
Apeksha Shah, Ajanta J. J. Raman, Dustin R. R. Neel, Caleb J. J. Kelly
Summary: This review highlights the contributions of women in the field of nutrition through analysis of biographical articles published in The Journal of Nutrition. It explores factors that facilitated and hindered their careers and emphasizes their scientific contributions.
CURRENT NUTRITION REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Eva P. Karasmanis, Janice M. Reimer, Agnieszka A. Kendrick, Kendrick H. V. Nguyen, Jennifer A. Rodriguez, Joey B. Truong, Indrajit Lahiri, Samara L. Reck-Peterson, Andres E. Leschziner
Summary: The contact sites between Lis1 and dynein, known as 'Chi', play a crucial role in regulating the activity of dynein. This study provides insights into the mechanism of how Lis1 relieves the autoinhibited state of dynein.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
