Article
Biochemistry & Molecular Biology
Ting Wang, Li Liu, Josef Voglmeir
Summary: Monoclonal antibodies have been successful in treating diseases, with recent research focused on the functional role and production methods of antibody N-glycosylation.
CARBOHYDRATE RESEARCH
(2022)
Review
Biotechnology & Applied Microbiology
Le Gao, Yi Jiang, Kai Hong, Xiaoyi Chen, Xin Wu
Summary: Protein glycosylation is a complex posttranslational modification process. Cellulases from fungi contain N-glycosylation and O-glycosylation. This study discusses the potential roles of glycosylation on cellulase characteristics and function. Glycoengineering can be used to control cellulase glycosylation by modifying the 3D structure through specially distributed glycans. Understanding the regulatory role of cellulase glycosylation will enable synthetic biology approaches for commercial cellulase development.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Mikel Garcia-Alija, Berre van Moer, Diego E. Sastre, Tala Azzam, Jonathan J. Du, Beatriz Trastoy, Nico Callewaert, Eric J. Sundberg, Marcelo E. Guerin
Summary: Antibody-based drugs, including IgG monoclonal antibodies, are widely used for treating cancer, autoimmune, and infectious diseases. Different glycoforms attached to antibodies can affect their efficacy, stability, and effector functions. Therefore, various antibody engineering strategies have been developed to produce antibodies with tailored glycoforms.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Multidisciplinary Sciences
Mingji Li, Xiaolu Zheng, Sudhanshu Shanker, Thapakorn Jaroentomeechai, Tyler D. Moeller, Sophia W. Hulbert, Ilkay Kocer, Josef Byrne, Emily C. Cox, Qin Fu, Sheng Zhang, Jason W. Labonte, Jeffrey J. Gray, Matthew P. DeLisa
Summary: The shotgun scanning glycomutagenesis strategy allows for rapid determination of glycosylatable sites in proteins and systematic investigation of the effects of glycan conjugation on protein structure and function. Experiment results show that stability and activity of many glycovariants are significantly altered by N-linked glycans, depending on the precise location of the modification.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Diana Chinyere Anyaogu, Anders Holmgaard Hansen, Jakob Blaesbjerg Hoof, Natalia Majewska, Fabiano Jares Contesini, Jackson T. Paul, Kristian Fog Nielsen, Timothy John Hobley, Shuang Yang, Hui Zhang, Michael Betenbaugh, Uffe Hasbro Mortensen
Summary: Filamentous fungi have the potential to secrete proteins efficiently for low-cost production, but the differences in N-glycosylation compared to humans pose a significant barrier for producing therapeutic proteins with appropriate glycosylation. Reprogramming the glycosylation pathway of fungi, such as Aspergilli, can help in decorating proteins with glycans that are more suitable for human acceptance. Further strain optimization may be necessary in order to achieve the desired glycan structures.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Shiva Izadi, Ulrike Vavra, Stanislav Melnik, Clemens Gruenwald-Gruber, Esther Foederl-Hoebenreich, Markus Sack, Kurt Zatloukal, Josef Gloessl, Eva Stoeger, Lukas Mach, Alexandra Castilho, Richard Strasser
Summary: Researchers engineered ACE2-Fc by modifying its glycan structure and obtained an ACE2-Fc variant with enhanced virus-neutralization activity, which shows promise as a potential drug candidate against coronavirus infections.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Oncology
Irene van der Haar Avila, Britt Windhouwer, Sandra J. J. van Vliet
Summary: Gangliosides are sialylated glycolipids that play a crucial role in cellular signaling. Changes in their composition during malignant transformation are associated with negative effects on patient survival. Gangliosides released by cancer cells in the tumor microenvironment have immunosuppressive activities and promote tumor progression. Understanding the role of gangliosides in immunomodulation and their potential as therapeutic targets is important for future immunotherapy regimens.
CANCER AND METASTASIS REVIEWS
(2023)
Article
Biotechnology & Applied Microbiology
Pavlos Kotidis, Roberto Donini, Johnny Arnsdorf, Anders Holmgaard Hansen, Bjorn Gunnar Rude Voldborg, Austin W. T. Chiang, Stuart M. Haslam, Michael Betenbaugh, Ioscani Jimenez del Val, Nathan E. Lewis, Frederick Krambeck, Cleo Kontoravdi
Summary: Researchers constructed a comprehensive glycosylation reaction network, CHOGlycoNET, based on 200 glycan datasets from CHO cell lines. They used machine learning and dimensionality reduction techniques to explore the distribution of mannosidase and glycosyltransferase enzymes in the Golgi apparatus and identify key reactions. CHOGlycoNET can accelerate glycomodel development and predict the effect of glycoengineering strategies.
METABOLIC ENGINEERING
(2023)
Review
Chemistry, Multidisciplinary
Kris Dammen-Brower, Paige Epler, Stanley Zhu, Zachary J. Bernstein, Paul R. Stabach, Demetrios T. Braddock, Jamie B. Spangler, Kevin J. Yarema
Summary: This article discusses the important role of glycosylation in therapeutic proteins and provides examples of drugs that have been improved through glycoengineering. As glycoengineering strategies develop, deliberate modification of therapeutic protein glycosylation will become more prevalent.
FRONTIERS IN CHEMISTRY
(2022)
Review
Microbiology
Linda A. Lieberman
Summary: Outer membrane vesicles (OMVs) derived from Gram-negative bacteria have various important roles and applications, including bacterial pathogenesis, cell-to-cell communication, gene transfer, quorum sensing, and maintenance of bacterial fitness. OMVs can be modified to express antigens, making them suitable for immunization and potential drug delivery. This review highlights the manipulation of OMVs, their use as vaccines, and novel engineering approaches to expand their applications.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Agnieszka Wyszynska, Rafal Jabluszewski
Summary: Bacterial glycoconjugates play diverse biological roles, with glycosyltransferases being key enzymes for glycosylation. Oligosaccharyltransferases, with broad substrate specificity, can be utilized to produce glycoconjugate vaccines.
ADVANCEMENTS OF MICROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Seth D. Ludwig, Zachary J. Bernstein, Christian Agatemor, Kris Dammen-Brower, Jeffrey Ruffolo, Jonah M. Rosas, Jeremy D. Post, Robert N. Cole, Kevin J. Yarema, Jamie B. Spangler
Summary: This study introduces a new glycoengineering strategy, leveraging atypical glycosylation patterns in variable domains of antibodies, to modulate the biological activities of therapeutic antibodies. Glycosylation sites were added to two monoclonal antibodies and computational tools were used to install N-glycosylation consensus sequences, creating glycovariants. Almost all the glycovariants were successfully glycosylated at the new sites without affecting the antibody's native function. Certain glycovariants exhibited modified activities compared to the parent antibody, demonstrating the potential of this strategy to modulate the biological function of antibodies involved in multi-component receptor systems.
Article
Biochemistry & Molecular Biology
Yen-Pang Hsu, Martin Frank, Debopreeti Mukherjee, Vladimir Shchurik, Alexey Makarov, Benjamin F. Mann
Summary: The glycans of SARS-CoV-2 spike protein play functional roles in the infection process and are highly conserved. Glycosylation of the receptor-binding domain (RBD) of the spike protein can be controlled and simplified through glycoengineering, affecting its behavior and interaction with host receptors. The antibody S309 can neutralize the impact of different RBD glycoforms on receptor binding affinity.
Article
Biotechnology & Applied Microbiology
Lukas Eidenberger, Florian Eminger, Alexandra Castilho, Herta Steinkellner
Summary: This study investigated the capability of three commonly used transient expression vectors to express a multi-component protein with controlled and modified N-glycosylation. The results showed that all three expression vectors produced similar quantities of targeted N-glycosylation, demonstrating the universal application of plant-based glycoengineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Julia Koenig-Beihammer, Ulrike Vavra, Yun-Ji Shin, Christiane Veit, Clemens Gruenwald-Gruber, Yasmin Gillitschka, Jasmin Huber, Manuela Hofner, Klemens Vierlinger, Dieter Mitteregger, Andreas Weinhaeusel, Richard Strasser
Summary: Glycosylation plays an important role in the infectivity and immune evasion of viral envelope proteins. The heavily glycosylated spike protein of SARS-CoV-2 is influenced by host-derived glycan modifications, which impact immunogenic epitopes, virus-cell interaction, and virus transmission. In this study, recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) were produced with blood group antigens using an in vivo glycoengineering approach in Nicotiana benthamiana plants. The glycosylation variants with blood group antigens were shown to be functional and could potentially provide protection against virus infection in non-infected RBD-negative blood group O individuals.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Lemonia Birikaki, Stephanie Pradeau, Sylvie Armand, Bernard Priem, Luis Marquez-Dominguez, Julio Reyes-Leyva, Gerardo Santos-Lopez, Eric Samain, Hugues Driguez, Sebastien Fort
CHEMISTRY-A EUROPEAN JOURNAL
(2015)
Article
Biotechnology & Applied Microbiology
Melanie Leroux, Bernard Priem
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Emeline Richard, Laurine Buon, Sophie Drouillard, Sebastien Fort, Bernard Priem
Article
Veterinary Sciences
Gerardo Santos-Lopez, Maria T. Borraz-Arguello, Luis Marquez-Dominguez, Juan Carlos Flores-Alonso, Humberto Ramirez-Mendoza, Bernard Priem, Sebastien Fort, Veronica Vallejo-Ruiz, Julio Reyes-Leyva, Irma Herrera-Carnacho
RESEARCH IN VETERINARY SCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Ludovic Bastide, Bernard Priem, Sebastien Fort
CARBOHYDRATE RESEARCH
(2011)
Article
Biochemistry & Molecular Biology
Helene Barreteau, Emeline Richard, Sophie Drouillard, Eric Samain, Bernard Priem
CARBOHYDRATE RESEARCH
(2012)
Article
Biochemistry & Molecular Biology
Elif Yavuz, Carola Maffioli, Karin Ilg, Markus Aebi, Bernard Priem
GLYCOCONJUGATE JOURNAL
(2011)
Article
Chemistry, Multidisciplinary
Stefan Kaufmann, Karin Ilg, Alireza Mashaghi, Marcus Textor, Bernard Priem, Markus Aebi, Erik Reimhult
Article
Multidisciplinary Sciences
Claire Debarnot, Yoan R. Monneau, Veronique Roig-Zamboni, Vincent Delauzun, Christine Le Narvor, Emeline Richard, Jerome Henault, Adeline Goulet, Firas Fadel, Romain R. Vives, Bernard Priem, David Bonnaffe, Hugues Lortat-Jacob, Yves Bourne
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Melanie Leroux, Julie Michaud, Eric Bayma, Sylvie Armand, Sophie Drouillard, Bernard Priem
Article
Biotechnology & Applied Microbiology
Sebastian Cerminati, Melanie Leroux, Pablo Anselmi, Salvador Peiru, Juan C. Alonso, Bernard Priem, Hugo G. Menzella
Summary: Hyaluronic acid, a valuable glycosaminoglycan, is widely used in health and cosmetic fields. Research shows that Bacillus subtilis could be a cost-effective host for HA production with potential economic benefits.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Correction
Biotechnology & Applied Microbiology
Sebastian Cerminati, Melanie Leroux, Pablo Anselmi, Salvador Peiru, Juan C. Alonso, Bernard Priem, Hugo G. Menzella
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Emeline Richard, Carlo Pifferi, Michele Fiore, Eric Samain, Audrey Le Gouellec, Sebastien Fort, Olivier Renaudet, Bernard Priem
Article
Chemistry, Applied
Bernard Priem, Julien Peroux, Philippe Colin-Morel, Sophie Drouillard, Sebastien Fort
CARBOHYDRATE POLYMERS
(2017)