Article
Chemistry, Multidisciplinary
A. R. Ortiz Moreno, R. Li, K. Wu, R. Schirhagl
Summary: Lipid peroxidation is a process where reactive oxygen species attack lipids within cell membranes, leading to toxic and carcinogenic products. Understanding this process at nanoscale resolution is important and challenging due to the short lifespan and reactivity of the radical intermediates involved. In this study, lipid peroxidation of three artificial lipid bilayers on a diamond substrate was investigated using a diamond quantum sensing method called T-1-relaxometry, allowing for in situ measurements and imaging of radical intermediates.
Article
Chemistry, Physical
Niki Baccile, Anyssa Derj, Cedric Boissiere, Vincent Humblot, Ariane Deniset-Besseau
Summary: The development of supported glycosylated lipid layers using microbial biosurfactants provides a versatile and robust method for preparing defect-free and homogeneous lipid monolayers. The method offers precise control of thickness below 10 nm and is compatible with aqueous solutions. Characterization techniques show that the glycosylated SLM are uniformly thick at 2.8 +/- 1.0 nm and defect-free at nanometer to centimeter scales.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Multidisciplinary
Rachel J. Goodband, Colin D. Bain, Margarita Staykova
Summary: This study compares the fusion of giant lipid and block-copolymer vesicles on different substrates and characterizes the properties of membrane patches and the formation of continuous supported membranes. Polymer membranes have lower fluidity and adjacent patches fail to fuse spontaneously, possibly due to hydration repulsion forces, which can be weakened by increasing the temperature.
Article
Chemistry, Physical
Alessandra Luchini, Federica Sebastiani, Frederik Gronbaek Tidemand, Krishna Chaithanya Batchu, Mario Campana, Giovanna Fragneto, Marite Cardenas, Lise Arleth
Summary: Peptide discs, resembling 10 nm discoidal lipid bilayers, can be used as precursors for Supported Lipid Bilayers (SLBs) without the need to alter any experimental variables, allowing for faster formation compared to vesicle fusion. SLBs can be successfully formed from both synthetic and natural lipid mixtures, with traces of 18A peptide at the support surface having no effect on the SLB structure.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Biophysics
Autumn A. Anthony, Osman Sahin, Murat Kaya Yapici, Daniel Rogers, Aurelia R. Honerkamp-Smith
Summary: This study systematically measured the coefficient of interleaflet friction in supported lipid membranes using a microfluidic technique. The results showed that this technique provides reproducible measurements of the friction coefficient and is sensitive enough to detect differences in friction between different lipid membranes. Adding cholesterol significantly increased interleaflet friction. Additionally, fluid shear stress was found to reversibly induce the gel phase in supported lipid bilayers close to the gel-transition temperature.
BIOPHYSICAL JOURNAL
(2022)
Article
Physics, Multidisciplinary
Shi Yu, Jianqiao Zhao, Ruizhi Chu, Xiao Li, Guoguang Wu, Xianliang Meng
Summary: This study presents mesoscale models for the anomalous diffusion of a polymer chain on a heterogeneous surface. The simulation results show that bead-spring chains exhibit sub-diffusion on charged lipid bilayers, while a simulated 17 base pairs double stranded DNA exhibits normal diffusion. The energy landscape experienced by short DNA chains is less heterogeneous compared to long DNA chains, resulting in different diffusion behaviors.
Article
Chemistry, Multidisciplinary
Hui Yin, Arielle C. Mensch, Christian A. Lochbaum, Isabel U. Foreman-Ortiz, Emily R. Caudill, Robert J. Hamers, Joel A. Pedersen
Summary: Supported lipid bilayers are important model systems for studying the interactions of proteins, peptides, and nanoparticles with biological membranes. The properties of the solid substrate, such as topography and coating, can affect the formation and properties of supported phospholipid bilayers. The interactions of nanoparticles with supported lipid bilayers depend on the substrate composition.
Article
Polymer Science
Che-Lun Chin, Lu-Jan Huang, Zheng-Xian Lu, Wei-Chun Weng, Ling Chao
Summary: This study demonstrates that the water absorption ability of dried PAAm hydrogel can facilitate the formation of hydrogel-SLBs. The high quality of the hydrogel surface is crucial for SLB formation, and minimizing oxygen exposure during drying can prevent the formation of an oxidized crust.
Article
Chemistry, Multidisciplinary
Claudia Lubrano, Ugo Bruno, Chiara Ausilio, Francesca Santoro
Summary: This study investigates the short-term depression of a biomembrane-based organic electrochemical transistor (OECT) through supported lipid bilayer mediation. The ionic barrier behavior of the lipid bilayer is utilized to enhance the neuromorphic operation of the OECT. These biomimetic neuromorphic devices pave the way for in vitro platforms resembling synapses to study and characterize synaptic plasticity loss in neurodegenerative diseases.
ADVANCED MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Nathan E. Thomas, Wei Feng, Katherine A. Henzler-Wildman
Summary: This study presents a new method for measuring transport stoichiometry using solid supported membrane electrophysiology (SSME), which greatly improves throughput and allows verification of specific transport ratios for various ion-coupled transporters.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Rashad Kariuki, Rowan Penman, Saffron J. Bryant, Rebecca Orrell-Trigg, Nastaran Meftahi, Russell J. Crawford, Chris F. McConville, Gary Bryant, Kislon Voitchovsky, Charlotte E. Conn, Andrew J. Christofferson, Aaron Elbourne
Summary: Nanomaterials have the potential to revolutionize biological and biomedical research, and this study provides detailed insights into how gold nanoparticles interact with phospholipid membranes. The study shows that the nanoparticles can be absorbed and internalized by fluid-phase membranes, and partially embed into gel-phase membranes, causing perturbations throughout the bilayers.
Article
Chemistry, Physical
Massimiliano Galluzzi, Lorenzo Marfori, Stefania Asperti, Alessandro De Vita, Matteo Giannangeli, Alessandro Caselli, Paolo Milani, Alessandro Podesta
Summary: Research has shown that Ionic Liquids (ILs) can restructure lipid bilayers by forming new ILs/lipid complexes, leading to distinctive morphological features such as increased area and roughness. Additionally, IL exposure can disrupt the structural ordering and rigidity of membranes, as evidenced by changes in breakthrough force, Young's modulus, and area stretching modulus. AFM analysis also detected alterations in the electrostatic double-layer at the membrane surface, indicating a progressive stratification of cations on the lipid membrane.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Nanoscience & Nanotechnology
Nicolo Paracini, Philipp Gutfreund, Rebecca Welbourn, Juan Francisco Gonzalez-Martinez, Kexin Zhu, Yansong Miao, Nageshwar Yepuri, Tamim A. . Darwish, Christopher Garvey, Sarah Waldie, Johan Larsson, Max Wolff, Marite Cardenas
Summary: Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We investigated the structure of lipid bilayers formed on nanoparticle arrays through vesicle fusion using scattering techniques and direct imaging. The presence of both flat and curved supported lipid bilayers on the same substrate, combined with the accuracy of grazing incidence neutron scattering, offers a promising approach to studying curvature-dependent membrane phenomena.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Eduart Gutierrez-Pineda, Patrizia Andreozzi, Eleftheria Diamanti, Ramiro Anguiano, Ronald F. Ziolo, Sergio E. Moya, Maria Jose Rodriguez-Presa, Claudio A. Gervasi
Summary: Supported Lipid Bilayers (SLBs) on Polyelectrolyte Multilayers (PEMs) have large potential as models for developing sensor devices. SLBs can be designed with receptors and channels to create a sensing interface for ions and biomarkers. The fabrication of valinomycin-doped SLBs on PEMs as a model system for biophysical studies and for selective ion sensing is studied.
BIOELECTROCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Zixuan Lu, Chiara Barberio, Ana Fernandez-Villegas, Aimee Withers, Alexandra Wheeler, Konstantinos Kallitsis, Eleonora Martinelli, Achilleas Savva, Becky M. Hess, Anna-Maria Pappa, Gabriele S. Kaminski Schierle, Roisin M. Owens
Summary: This work reports a new system that integrates neuron membranes with organic microelectrode arrays for drug studies targeting neuronal ion channels. The system overcomes the challenges of traditional methods and provides an easy-to-test, rapid, ultra-sensitive, cell-free, and high-throughput platform to monitor dose-dependent ion-channel blocking effects on native neuronal membranes.
Article
Nanoscience & Nanotechnology
Elia Roma, Pietro Corsi, Max Willinger, Nikolaus Simon Leitner, Ronald Zirbs, Erik Reimhult, Barbara Capone, Tecla Gasperi
Summary: This study presents a comprehensive approach using experimental, theoretical, and computational methods to design macromolecules capable of adsorbing cargo at the nanoscale. The experimental results validate the successful design process and confirm the accuracy of theoretical predictions.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Multidisciplinary Sciences
Katharina R. Lenhardt, Hergen Breitzke, Gerd Buntkowsky, Erik Reimhult, Max Willinger, Thilo Rennert
Summary: The study reveals that the structure of SROAS has a significant impact on their aggregation effects and properties, with different compositions of SROAS showing varying degrees of surface loss due to aggregation upon freezing. Cryomilling effectively fractures aggregates but does not change the magnitude of specific surface area, indicating a structure-dependent effect on spatial arrangement at the submicron scale. Understanding the physical and chemical processes of aggregation as a function of composition and crystallinity may enhance our knowledge of the reactivity of SROAS in the environment.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Tomasz Clapa, Jakub Michalski, Anna Syguda, Dorota Narozna, Peter van Oostrum, Erik Reimhult
Summary: Pseudomonas aeruginosa is a multi-drug resistant pathogen classified as one of the most life-threatening pathogens. Ionic liquids are a promising class of novel antibiotics that could effectively inhibit bacterial growth and reduce strain fitness and virulence factor production in P. aeruginosa infections.
RESEARCH IN MICROBIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Bernadette Lechner, Simone Hageneder, Katharina Schmidt, Mark P. Kreuzer, Rick Conzemius, Erik Reimhult, Ivan Barisi, Jakub Dostalek
Summary: The growth of surface-attached single-stranded DNA chains is monitored using an evanescent wave optical biosensor that combines surface plasmon resonance and optical waveguide spectroscopy. This technique allows for accurate measurement of the thickness, volume content, and density gradients of the DNA brushes in real time, providing important insights for sensitive and rapid assays in affinity-based biosensors.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Physical
Omar Abdelaziz Ouni, Guruprakash Subbiahdoss, Andrea Scheberl, Erik Reimhult
Summary: The DNA polyelectrolyte multilayer coatings can reduce infection risk while promoting the attachment of host tissue cells on medical implants. These coatings reduce bacterial adhesion, alleviate the risk of implant infections, and have no cytotoxic effect on SaOS-2 cells.
Article
Multidisciplinary Sciences
Alireza Mashaghi, Roland van der Veen
Summary: The topological framework of circuit topology has been introduced to complement knot theory and help in understanding the physics of molecular folding. Circuit topology captures the arrangements of intra-chain contacts within a given folded linear chain and allows for the classification and comparison of chains. Polynomial invariants have been presented that are efficient and powerful enough to deal with any combination of soft and hard contacts.
Article
Nanoscience & Nanotechnology
Maksim Y. Livshits, Jianzhong Yang, Fahimeh Maghsoodi, Andrea Scheberl, Samuel M. Greer, Mohammed Khalil, Edward Strach, Dylan Brown, Benjamin W. Stein, Erik Reimhult, Jeffrey J. Rack, Eva Chi, David G. Whitten
Summary: The study investigates the photochemical properties and antimicrobial activity of a novel P3HT-Im/SDS material, indicating that SDS complexation with P3HT-Im reduces ROS generation but still maintains some antimicrobial activity. The presence of non-aggregated or lightly SDS-covered polymer segments can still generate ROS, while full encapsulation of the polymer deactivates the light killing pathway. High SDS concentrations deactivate all antimicrobial activity, even though the polymer retains its ability to generate ROS.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemical Research Methods
Shokoofeh Maghari, Erik Reimhult, Parviz Ghezellou, Alireza Ghassempour
Summary: The present study evaluated the influence of surface properties of nanoparticles (NPs) on the intensity of mass signals in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and laser desorption/ionization mass spectrometry (LDI-MS) experiments. The results indicated that the surface ligands of NPs significantly enhanced the signal intensity based on electrostatic interaction and laser energy absorption in LDI-MS analyses. In MALDI-MS experiments, achieving colloidal stability of NPs in the matrix solution led to a significant signal improvement.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Instruments & Instrumentation
Tayebeh Saghaei, Erik Reimhult, Peter D. J. van Oostrum
Summary: We present a technique for rapidly changing the contents of a small sample chamber with minimal sample consumption. Our design eliminates dead volume and minimizes mixing, making it an exciting alternative to traditional solutions. It also functions as an efficient bubble trap.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2022)
Article
Chemistry, Physical
Max Willinger, Martin Felhofer, Erik Reimhult, Ronald Zirbs
Summary: The hydrothermal coating process using TEOS achieves higher yields and thicker silica coatings compared to conventional methods, resulting in more uniform shells with higher density, making it the method of choice when mechanical integrity and low permeability of the coating are required.
Article
Chemistry, Multidisciplinary
Antonino Puglisi, Simone Bassini, Erik Reimhult
Summary: Cholesterol plays a crucial role in major cardiovascular and neurodegenerative diseases, and cyclodextrins have shown promising therapeutic efficacy in sequestering and mobilising cholesterol. A new approach involves core-shell superparamagnetic iron oxide nanoparticles functionalised with cyclodextrins, which can specifically cleave off from the nanoparticles' shell at a slightly acidic pH to mobilise cholesterol out of the lysosome. This platform shows potential in removing cholesterol from the lysosomal compartment.
FRONTIERS IN CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Katharina Schmidt, Simone Hageneder, Bernadette Lechner, Barbara Zbiral, Stefan Fossati, Yasaman Ahmadi, Maria Minunni, Jose Luis Toca-Herrera, Erik Reimhult, Ivan Barisic, Jakub Dostalek
Summary: This study tailored rolling circle amplification (RCA) for affinity biosensors relying on optical probing with confined surface plasmon field. By combining surface plasmon resonance (SPR) and surface plasmon-enhanced fluorescence (PEF) methods, the impact of long RCA-generated single-stranded DNA (ssDNA) chains on sensor response enhancement was investigated. The reported RCA implementation improved the limit of detection (LOD) for model target analyte, showing enhancement in sensor response and potential for single-molecule detection.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Antonino Puglisi, Noemi Bognanni, Graziella Vecchio, Ece Bayir, Peter van Oostrum, Dawn Shepherd, Frances Platt, Erik Reimhult
Summary: Core-shell superparamagnetic iron oxide nanoparticles have great potential as a therapeutic and diagnostic platform in biological systems. This study investigates the biological effects of multifunctional cyclodextrin-appended SPIONs (CySPION) in mutant Npc1-deficient CHO cells. The results show that CySPIONs have low cytotoxicity, are strongly endocytosed, and localized in the lysosomal compartment. The pH-sensitive chemistry of these nanoparticles allows for the release of monomeric cyclodextrins, promoting the removal of accumulated cholesterol. Additionally, CySPIONs demonstrate high transport across blood-brain barrier models, suggesting their potential as a therapeutic approach for cholesterol-related brain diseases.
Article
Microbiology
Guruprakash Subbiahdoss, Sarah Osmen, Erik Reimhult
Summary: The Komagataeibacter bacteria in kombucha can produce cellulosic biofilms using hydrocarbons as the sole source of carbon and energy. The presence of biosurfactants in kombucha leads to a faster decrease in interfacial tension and the formation of stable and elastic biofilm membranes.
Article
Nanoscience & Nanotechnology
Nikolaus Simon Leitner, Martina Schroffenegger, Erik Reimhult
Summary: Nanoparticles play a crucial role in life science and biomedicine, with their fate in biological systems determined by protein corona and influenced by their design. Biomedical nanoparticles with core-shell structure can prevent protein corona formation through polymer shell, providing a highly repulsive barrier.
ACS APPLIED BIO MATERIALS
(2021)
