Article
Microbiology
Anthony V. Morante, Daniel Davidnan Baboolal, Xavier Simon, Elizabeth, Mark Cheng-Ying Pan, Patricio I. Meneses
Summary: The amino acid residues 43-DQILQ-47 of the HPV16 L2 protein play a crucial role in the intracellular trafficking of the virus, particularly in trafficking beyond the endoplasmic reticulum.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Baicheng Huang, Yufeng Lou, Zihan Zeng, Xingchi Kan, Xinping Shi, Yue Wu, Ling Guo, Muzhen Wang, Xingxu Huang, Xuemei Tian, Xinjie Wang
Summary: A Cas12a-based fluorescent microfluidic detection system was developed for rapid and sensitive detection of six HPV subtypes. The assay showed consistent results with the qPCR assay when tested with clinical samples, holding promise for improving cervical cancer screening and prevention.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Alexa N. Roth, Pavithra Aravamudhan, Isabel Fernandez de Castro, Raquel Tenorio, Cristina Risco, Terence S. Dermody
Summary: The function of intracellular vesicular transport systems in reovirus entry, trafficking, and egress is discussed, with a focus on designing strategic therapeutic interventions. Understanding the mechanisms by which viruses coopt vesicular trafficking may lead to the development of antiviral agents.
TRENDS IN MICROBIOLOGY
(2021)
Review
Virology
Snjezana Mikulicic, Johannes Strunk, Luise Florin
Summary: During initial infection, HPV takes a unique trafficking pathway through host cells, involving specific endocytosis and retrograde trafficking processes. Cellular reorganization during mitosis enables further virus transport to establish infection while evading cellular immune defenses.
Article
Virology
David Pim, Justyna Broniarczyk, Abida Siddiqa, Paola Massimi, Lawrence Banks
Summary: Previous studies have identified the involvement of sorting nexins 17 and 27, as well as the retromer complex, in HPV infection. This study reveals the significant role of the newly identified retriever complex and provides insight into how both SNXs contribute to retromer and retriever recruitment during infection.
JOURNAL OF VIROLOGY
(2021)
Article
Biology
Priscilla S-W Yeung, Megumi Yamashita, Murali Prakriya
Summary: Ca2+ release-activated Ca2+ (CRAC) channels are activated by direct physical interactions between Orai1 and STIM1. The L138F gain-of-function mutation in Orai1 causes tubular aggregate myopathy due to steric clash between L138 and T92. Surprisingly, the strongly activating L138 and T92 mutations exhibit Ca2+-dependent inactivation (CDI) in the absence of STIM1, contradicting prevailing views.
Article
Multidisciplinary Sciences
Christopher M. Richards, Sabrina Jabs, Wenjie Qiao, Lauren D. Varanese, Michaela Schweizer, Peter R. Mosen, Nicholas M. Riley, Malte Klussendorf, James R. Zengel, Ryan A. Flynn, Arjun Rustagi, John C. Widen, Christine E. Peters, Yaw Shin Ooi, Xuping Xie, Pei-Yong Shi, Ralf Bartenschlager, Andreas S. Puschnik, Matthew Bogyo, Carolyn R. Bertozzi, Catherine A. Blish, Dominic Winter, Claude M. Nagamine, Thomas Braulke, Jan E. Carette
Summary: LYSET is a critical factor in the M6P trafficking machinery of cells, and mutations in LYSET can explain the phenotype of the associated disorder.
Article
Multidisciplinary Sciences
Nell Saunders, Ignacio Fernandez, Cyril Planchais, Vincent Michel, Maaran Michael Rajah, Eduard Baquero Salazar, Jeanne Postal, Francoise Porrot, Florence Guivel-Benhassine, Catherine Blanc, Gaelle Chauveau-Le Friec, Augustin Martin, Ludivine Grzelak, Rischa Maya Oktavia, Annalisa Meola, Olivia Ahouzi, Hunter Hoover-Watson, Matthieu Prot, Deborah Delaune, Marion Cornelissen, Martin Deijs, Veronique Meriaux, Hugo Mouquet, Etienne Simon-Loriere, Lia van der Hoek, Pierre Lafaye, Felix Rey, Julian Buchrieser, Olivier Schwartz
Summary: In this study, it was found that TMPRSS2 is a functional receptor for coronavirus HKU1, triggering cell-cell fusion and viral entry. Designed anti-TMPRSS2 nanobodies were shown to inhibit HKU1 spike attachment and infection.
Article
Fisheries
Ling Shao, Jiuru Zhao, Qian Tang
Summary: The study identified that non-muscle myosin heavy chain 9 (Myh9) plays a crucial role in the entry of IPNV by interacting with viral outer capsid protein VP2. Treatments with different Myh9 inhibitors suppressed IPNV infection, while overexpression of Myh9 promoted viral entry. Additionally, IPNV infection induced the formation of intracellular nanotubes which the virus could utilize for cell-to-cell movement.
Article
Virology
Mac Crite, Daniel DiMaio
Summary: Intracellular trafficking of human papillomavirus (HPV) during virus entry requires the involvement of cellular protease gamma-secretase, which interacts with the minor capsid protein L2 and facilitates its insertion into the endosomal membrane in order to bind other cellular factors necessary for proper virus trafficking. The TM domain of HPV L2 is crucial for its protrusion into the cytoplasm, and mutational studies have shown that the infectivity of HPV mutants is closely correlated with gamma-secretase binding and stabilization, highlighting the importance of the native TM domain of HPV L2 for virus entry.
Article
Biochemistry & Molecular Biology
An-Jen Chiang, Chia-Jung Li, Kuan-Hao Tsui, Chung Chang, Yuan-chin Ivan Chang, Li-Wen Chen, Tsung-Hsien Chang, Jim Jinn-Chyuan Sheu
Summary: The study found that UBE2C is highly expressed in human cervical squamous cell carcinoma (CESC) tissues and is related to clinical characteristics. In vitro experiments demonstrated that overexpression and knockdown of UBE2C respectively enhanced and reduced proliferation of cervical cancer cells. In vivo experiments showed that UBE2C regulates the expression and activity of the mTOR/PI3K/AKT pathway.
Review
Immunology
Giovanna Milano, Giovanni Guarducci, Nicola Nante, Emanuele Montomoli, Ilaria Manini
Summary: Human papillomavirus (HPV) is a common sexually transmitted infection (STI) and the most common viral STI. Vaccination is an effective public health strategy for preventing HPV-related diseases. There are currently three types of vaccines available, all targeting the most oncogenic virus genotypes (HPV 16 and 18). Including all genders in vaccination programs is important for achieving herd immunity against HPV.
Review
Virology
Ting Wang, Lilan Zheng, Qiujian Zhao, Yazhou Yao, Feng Zhou, Fang Wei, Qiliang Cai
Summary: PIV5 is a negative-sense, single-stranded RNA virus that can infect humans and many species of animals. It has shown promise as a vector for developing vaccines against various human infectious diseases. This review summarizes recent progress and highlights the advantages and strategies of PIV5 as a vaccine vector for future clinical trials.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Oncology
Chahrazade Kantari-Mimoun, Sarah Barrin, Lene Vimeux, Sandrine Haghiri, Claire Gervais, Sandy Joaquina, Joerg Mittelstaet, Nadine Mockel-Tenbrinck, Ali Kinkhabwala, Diane Damotte, Audrey Lupo, Mathilde Sibony, Marco Alifano, Elisabetta Dondi, Nadege Bercovici, Alain Trautmann, Andrew D. Kaiser, Emmanuel Donnadieu
Summary: The study found that CD20 CAR-T cells rapidly formed effective conjugates with target cells, while EGFR CAR-T cells need to migrate to the center of tumor cell regions after initial activation at the periphery of tumor islets. Activated CAR-T cells induce upregulation of ICAM-1 on malignant tumor cells via an IFN gamma-dependent pathway.
CANCER IMMUNOLOGY RESEARCH
(2021)
Article
Public, Environmental & Occupational Health
Xinye Wang, Leshan Xiu, Raquel A. Binder, Teck-Hock Toh, Jeffrey Soon-Yit Lee, Jakie Ting, Son T. Than, Wenhao Qi, Kristen K. Coleman, David Perera, Maijuan Ma, Gregory C. Gray
Summary: Through using a new panspecies coronavirus molecular assay, this study examined animal, human, and environmental samples, detecting various coronaviruses. The RT-PCR assay showed potential as a screening diagnostic for early detection of coronavirus incursions or prepandemic virus emergence in animal or human populations.