Improved antibiotic-free plasmid vector design by incorporation of transient expression enhancers

Critical design criteria for minimal antibiotic-free plasmid vectors necessary to combine robust RNA Pol II and Pol III-mediated eukaryotic expression with high bacterial production yields

(2010) Aaron E. Carnes et al.   JOURNAL OF GENE MEDICINE

Plasmid DNA vaccine vector design: Impact on efficacy, safety and upstream production

(2009) James A. Williams et al.   BIOTECHNOLOGY ADVANCES

Plasmid DNA production combining antibiotic-free selection, inducible high yield fermentation, and novel autolytic purification

(2009) Aaron E. Carnes et al.   BIOTECHNOLOGY AND BIOENGINEERING

Generic plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes

(2009) James A. Williams et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identification and Functional Characterization of Cytoplasmic Determinants of Plasmid DNA Nuclear Import

(2009) Felix M. Munkonge et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Robust, Vaccine-Induced CD8 + T Lymphocyte Response against an Out-of-Frame Epitope

(2009) Nicholas J. Maness et al.   JOURNAL OF IMMUNOLOGY

Identification of Protein Cofactors Necessary for Sequence-specific Plasmid DNA Nuclear Import

(2009) Aaron M Miller et al.   MOLECULAR THERAPY

Nucleofection of DCs to Generate Multivirus-specific T Cells for Prevention or Treatment of Viral Infections in the Immunocompromised Host

(2009) Ulrike Gerdemann et al.   MOLECULAR THERAPY

Electroporation for the Delivery of DNA-based Vaccines and Immunotherapeutics: Current Clinical Developments

(2009) Angela M Bodles-Brakhop et al.   MOLECULAR THERAPY

Evaluating post-transcriptional regulatory elements for enhancing transient gene expression levels in CHO K1 and HEK293 cells

(2009) Mariati et al.   PROTEIN EXPRESSION AND PURIFICATION

Improved antibiotic-free DNA vaccine vectors utilizing a novel RNA based plasmid selection system

(2009) Jeremy Luke et al.   VACCINE

Effect of trimerization motifs on quaternary structure, antigenicity, and immunogenicity of a noncleavable HIV-1 gp140 envelope glycoprotein

(2009) Sean X. Du et al.   VIROLOGY

Age-dependent impairment of HIF-1α expression in diabetic mice: Correction with electroporation-facilitated gene therapy increases wound healing, angiogenesis, and circulating angiogenic cells

(2008) Lixin Liu et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Metastases suppressor NM23-H2 interaction with G-quadruplex DNA within c-MYC promoter nuclease hypersensitive element induces c-MYC expression

(2008) Ram Krishna Thakur et al.   NUCLEIC ACIDS RESEARCH

Nuclear delivery of NFκB-assisted DNA/polymer complexes: plasmid DNA quantitation by confocal laser scanning microscopy and evidence of nuclear polyplexes by FRET imaging

(2008) Gilles Breuzard et al.   NUCLEIC ACIDS RESEARCH