Article
Biochemistry & Molecular Biology
Hang Dong, Jian Li, Xueyuan Huang, Haiting Liu, Rong Gui
Summary: This study developed a novel multifunctional hydrogel, Pltm@CNPs/Gel, which incorporates platelet membrane camouflaged cerium nanoparticles into a gelatin methacryloyl matrix. The Pltm@CNPs/Gel hydrogel possesses anti-oxidative and anti-inflammatory properties and accelerates diabetic wound healing by promoting neovascularization, cell proliferation, and collagen fiber organization. The hydrogel shows potential as a therapeutic option for diabetic wound healing and as a diabetic wound dressing candidate.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Xiang Zhang, Dan Yao, Weiyi Zhao, Rui Zhang, Bingran Yu, Guiping Ma, Yang Li, Daifeng Hao, Fu-Jian Xu
Summary: The study developed an engineered PRP dual-network hydrogel (DN gel) based on sodium alginate, which has improved gelling property and sustained release of growth factors, showing great potential for the next generation of PRP-based bioactive wound dressings.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Meral Nadra, Wanting Niu, Motoichi Kurisawa, Dominique Rousson, Myron Spector
Summary: In implant dentistry, the difficulties of reconstructing large defects in the oral cavity and musculoskeletal system using porous blocks have been addressed by developing a gelatin-based gel incorporating platelet-rich plasma lysate. This gel acts as a scaffold for cell migration and a vehicle for sustained release of therapeutic agents, promoting bone reconstruction.
BIOENGINEERING-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Sarah Yahia, Islam A. Khalil, Ibrahim M. El-Sherbiny
Summary: This study aimed at developing a bioimplant for the treatment of damaged intervertebral discs that can maintain their function. The implant, composed of a fortified gelatin-based hydrogel, mimics the mechanical properties of intervertebral discs and releases bioactive agents. Experimental results showed that the implant has good biocompatibility and promotes healing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biophysics
Ting Wang, Wanwan Yi, Yu Zhang, Hang Wu, Hengwei Fan, Jiulong Zhao, Shige Wang
Summary: In recent years, the healing of chronic wounds has become a challenging problem in clinical practice. This study aimed to address the challenges in wound care by designing a dual-network hydrogel composed of sodium alginate and platelet-rich plasma. The hydrogel showed a three-dimensional network structure and exhibited the potential for cell proliferation and vascular regeneration. When applied to wound skin in rats, the hydrogel effectively promoted wound closure. Overall, this strategy offers a simple and feasible approach for overcoming the shortcomings of conventional wound dressings.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Jon Mercader Ruiz, Maider Beitia, Diego Delgado, Pello Sanchez, Maria Jesus Arnaiz, Leonor Lopez de Dicastillo, Fernando Benito-Lopez, Lourdes Basabe-Desmonts, Mikel Sanchez
Summary: Platelet-rich plasma (PRP) is a biologic product commonly used in medicine for tissue repair. This study developed a new method to obtain PRP enriched in both platelet and extraplatelet molecules using hydroxyethyl acrylamide (HEAA)-based hydrogels. The resulting PRP showed higher levels of extraplatelet growth factors and improved cell viability compared to standard PRP. This absorption-based method has the potential to improve tissue regeneration capacity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Bianza Moise Bakadia, Abeer Ahmed Qaed Ahmed, Lallepak Lamboni, Zhijun Shi, Biampata Mutu Mukole, Ruizhu Zheng, Mazono Pierre Mbang, Bi Zhang, Mario Gauthier, Guang Yang
Summary: The management of diabetic wounds is a challenge, but platelet-rich plasma (PRP) gel, PRP-derived exosomes (PRP-Exos), and mesenchymal stem cell-derived exosomes (MSC-Exos) have potential in wound treatment. However, their limitations include poor mechanical properties, short half-lives of growth factors (GFs), burst release of GFs and exosomes, and protease degradation. To overcome these limitations, dual-crosslinked hydrogels based on silk protein (SP) were developed, which showed improved mechanical properties and sustained release of GFs and exosomes. These hydrogels promoted diabetic wound healing by upregulating GF expression, down-regulating matrix metalloproteinase-9 expression, and promoting angiogenesis and re-epithelialization.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Ching-Cheng Tsai, Tai-Horng Young, Guang-Shih Chen, Nai-Chen Cheng
Summary: A chitosan/gelatin hydrogel crosslinked by glyoxal was fabricated for sustained release of HPL, showing significant promotion of cell proliferation and migration. The growth factors released from the hydrogel exhibited sustained release characteristics, and HPL encapsulation also stimulated angiogenesis in ovo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Shangzhi Li, Qi Dong, Xiaotong Peng, Yun Chen, Hongjun Yang, Weilin Xu, Yanteng Zhao, Pu Xiao, Yingshan Zhou
Summary: A self-healing nanocomposite hydrogel based on hyaluronic acid has been developed, which exhibits good strength, self-healing ability, and the potential to load platelet-rich plasma (PRP) for promoting skin wound healing.
Article
Medicine, General & Internal
Jon Mercader Ruiz, Maider Beitia, Diego Delgado, Pello Sanchez, Jorge Guadilla, Cristina Perez de Arrilucea, Fernando Benito-Lopez, Lourdes Basabe-Desmonts, Mikel Sanchez
Summary: This study developed a new ultrafiltration technique for obtaining Platelet-Rich Plasma (PRP). The technique was able to concentrate growth factors and cytokines present in plasma, resulting in improved effectiveness compared to conventional PRPs.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Roberta Sessa Stilhano, Priscila Martins Andrade Denapoli, Camila Congentino Gallo, Vivian Yochiko Samoto, Sheila Jean McNeill Ingham, Rene Jorge Abdalla, Timothy Jon Koh, Sang Won Han
Summary: This study aimed to investigate the effects of PPRP and LPRP on recovery from limb ischemia and growth factor expression. The results showed that both PRPs improved muscle fiber size and strength, reduced fibrosis and adipocytes, and did not significantly affect regenerative activities in the ischemic limb. In cell cultures, both PRPs were able to recover cell metabolism affected by serum deprivation.
Article
Biotechnology & Applied Microbiology
Shijia Tang, Lin Wang, Yunyang Zhang, Feimin Zhang
Summary: Repairing bone defects caused by trauma or diseases is a primary focus of prosthodontics. Researchers developed a novel biomimetic hydrogel by combining methacrylated alginate and 4-arm poly (ethylene glycol)-acrylate (4A-PEGAcr). The hydrogel was then incorporated with platelet-rich plasma (PRP) and biomineralized to promote bone tissue regeneration. In vitro and in vivo studies showed that the hydrogel had excellent biocompatibility and osteogenic activities.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Rita I. R. Ibanez, Ronaldo J. F. C. do Amaral, Christopher R. Simpson, Sarah M. Casey, Rui L. Reis, Alexandra P. Marques, Ciara M. Murphy, Fergal J. O'Brien
Summary: This study successfully incorporated PRP into a bioink for 3D printing regenerative scaffolds with controlled release of growth factors, showing superior angiogenic potential without inducing myofibroblastic phenotype in fibroblasts. This disruptive technology allows for personalized treatment with enhanced vascularization in tissue engineering applications.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Materials Science, Biomaterials
Qiu-Hao Shu, Rong-Tai Zuo, Min Chu, Jing-Jing Shi, Qin-Fei Ke, Jun-Jie Guan, Ya-Ping Guo
Summary: This study developed a therapeutic hydrogel for diabetic wound healing by encapsulating platelet-rich plasma exosomes (PRP-EXOs) in fiber-reinforced gelatin (GEL)/8-cyclodextrin (8-CD) hydrogels. The hydrogels provided a biocompatible microenvironment for cell adhesion, proliferation, and skin tissue regeneration, while the PRP-EXOs activated autophagy and inhibited apoptosis, promoting blood vessel creation and wound healing.
BIOMATERIALS ADVANCES
(2023)
Article
Cell Biology
Min Kyoung Kim, Jung Ah Yoon, Sook Young Yoon, Mira Park, Woo Sik Lee, Sang Woo Lyu, Haengseok Song
Summary: PRP treatment significantly improves symptoms in mice with AS, reducing fibrotic markers expression, alleviating oligo/amenorrhea phenotype, restoring fertility, and eliminating abnormalities such as aberrantly delayed embryo implantation and intrauterine growth retardation.
Article
Cell & Tissue Engineering
Shuichi Ogino, Michiharu Sakamoto, Sunghee Lee, Hiroki Yamanaka, Itaru Tsuge, Jun Arata, Yuki Sakamoto, Yusuke Kambe, Tetsuji Yamaoka, Naoki Morimoto
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Article
Biotechnology & Applied Microbiology
Toshihito Mitsui, Naoki Morimoto, Atsushi Mahara, Sharon Claudia Notodihardjo, Tien Minh Le, Maria Chiara Munisso, Natsuko Kakudo, Tetsuji Yamaoka, Kenji Kusumoto
BIOMED RESEARCH INTERNATIONAL
(2020)
Article
Multidisciplinary Sciences
Michiharu Sakamoto, Shuichi Ogino, Yoshihiro Shimizu, Masukazu Inoie, Sunghee Lee, Hiroki Yamanaka, Itaru Tsuge, Susumu Saito, Naoki Morimoto
Review
Pediatrics
Motoki Katsube, Shigehito Yamada, Natsuko Utsunomiya, Naoki Morimoto
Summary: The face is a small complex three-dimensional structure composed of various bones and essential organs. Geometric morphometrics (GM) uses shape configurations to retain geometric information and help visualize results, making it advantageous in morphometric studies of congenital facial anomalies.
CONGENITAL ANOMALIES
(2022)
Article
Cell & Tissue Engineering
A. Shoji-Pietraszkiewicz, M. Sakamoto, M. Katsube, S. Ogino, I Tsuge, H. Yamanaka, J. Arata, N. Morimoto
Summary: In this study, cultured epithelial autografts (CEA) were applied after curettage or abrasion of giant congenital melanocytic nevi (GCMN). The results showed successful growth of CEA on wounds and improvement in lesion color. Histopathological examination revealed regenerated dermis without nevus cells.
REGENERATIVE THERAPY
(2021)
Article
Cell & Tissue Engineering
Naoki Morimoto, Toshihito Mitsui, Yasuhiro Katayama, Natsuko Kakudo, Shuichi Ogino, Itaru Tsuge, Michiharu Sakamoto, Masakatsu Hihara, Kenji Kusumoto
Summary: CEA appears to accelerate epithelization after curettage or ablation of CMN, and can significantly improve the color of CMN when applied initially. The color of the target nevus improved significantly in both the Curettage group and non-Curettage group with initial treatment, with no significant differences in epithelization period or incidence of hypertrophic scars among the groups.
REGENERATIVE THERAPY
(2021)
Article
Cell & Tissue Engineering
Yuanjiaozi Li, Eiichi Sawaragi, Michiharu Sakamoto, Takashi Nakano, Hiroki Yamanaka, Itaru Tsuge, Kumiko Matsuno, Yasuhiko Tabata, Naoki Morimoto
Summary: The study concluded that Genocel (R) can serve as an equivalent skin substitute for wound healing, demonstrating comparable performance to conventional substitutes. Additionally, Genocel (R) exhibited the ability to accelerate capillary formation, making it a more suitable treatment option for chronic skin ulcers such as diabetic ulcers.
REGENERATIVE THERAPY
(2022)
Article
Cell & Tissue Engineering
Takashi Nakano, Michiharu Sakamoto, Yasuhiro Katayama, Yoshihiro Shimizu, Masukazu Inoie, Yuanjiaozi Li, Hiroki Yamanaka, Itaru Tsuge, Susumu Saito, Naoki Morimoto
Summary: Autologous cultured epidermis (CE) is an effective approach for treating extensive burns, but its production time is too long. Allogeneic CE can be prepared in advance and used as a wound dressing to promote wound healing. Dried CE, prepared by removing all water and viable cells, accelerates wound healing and may be a new therapeutic strategy. This study evaluated the safety and efficacy of dried CE in wound healing using a miniature swine model.
REGENERATIVE THERAPY
(2023)
Article
Surgery
Shinsuke Akita, Hiraku Kumamaru, Hisashi Motomura, Nobuyuki Mitsukawa, Naoki Morimoto, Minoru Sakuraba
Summary: This study analyzed data from 19,482 free-flap surgeries performed at 407 facilities in Japan and found that free-flap surgeries performed at institutions with a low average annual number of procedures had a higher risk of total necrosis. It is recommended to either aggregate cases into high-volume centers or improve management at low-volume centers.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2023)
Letter
Surgery
Itaru Tsuge, Nobuyuki Mitsukawa, Takane Suzuki, Shinsuke Akita, Yoshimichi Imai, Koichi Omori, Naoki Morimoto
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2023)
Article
Surgery
Itaru Tsuge, Maria Chiara Munisso, Tomoko Kosaka, Ayako Takaya, Yoshihiro Sowa, Chang Liu, Goshiro Yamamoto, Susumu Saito, Naoki Morimoto
Summary: This study successfully visualized subcutaneous arteries using photoacoustic tomography and compared the results with intraoperative fluorescence angiography, with a matching rate of 71.3-82.1%. The results of this study demonstrate that photoacoustic tomography can aid in selecting perforators for abdominal flap surgery.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2023)
Article
Cell & Tissue Engineering
Takashi Nakano, Hiroki Yamanaka, Michiharu Sakamoto, Itaru Tsuge, Yasuhiro Katayama, Susumu Saito, Jiro Ono, Tetsuji Yamaoka, Naoki Morimoto
Summary: This study successfully developed a self-assembled, cell-based dermal substitute derived from human dermal fibroblasts through long-term three-dimensional culture. The scaffold-free cultured dermis exhibited superior properties, such as sufficient tensile strength, high biocompatibility, and efficacy in promoting wound healing when compared to the collagen-based artificial dermis.
TISSUE ENGINEERING PART A
(2023)
Article
Cell & Tissue Engineering
Qiannan Zhao, Shuichi Ogino, Sunghee Lee, Yuki Kato, Yuanjiaozi Li, Michiharu Sakamoto, Hiroki Yamanaka, Takashi Nakano, Eiichi Sawaragi, Naoki Morimoto
Summary: Poly-L-lactic acid (PLLA) mesh implants combined with collagen sponge (CS) were replaced with autologous adipose tissue for regeneration. The study compared different combinations of external frames (P (LA/CL), PLLA, LMWPLLA) and internal fillings (PGA nanosheets, CS) and found that PLLA served as a promising external frame material while PGA nanosheets were an effective alternative internal filling material for adipose tissue regeneration.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Motoki Katsube, Natsuko Utsunomiya, Yasuhiro Katayama, Hiroki Yamanaka, Itaru Tsuge, Yoshihiro Sowa, Michiharu Sakamoto, Naoki Morimoto
Summary: This study demonstrates that the use of bFGF-CGS in primary palatoplasty can significantly reduce the occurrence of post-operative oronasal fistulas.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Tien Minh Le, Naoki Morimoto, Nhung Thi My Ly, Toshihito Mitsui, Sharon Claudia Notodihardjo, Shuichi Ogino, Jun Arata, Natsuko Kakudo, Kenji Kusumoto
Summary: MSCs have become a focus of attention in tissue repair and wound healing. Recent studies have shown the importance of apoptosis in MSC-mediated immunomodulation. High hydrostatic pressure treatment induced complete apoptosis in MSCs, offering potential for manipulated products in cell-based therapies.
STEM CELL REVIEWS AND REPORTS
(2021)