Article
Biochemistry & Molecular Biology
Dominika Kanikowska, Alina Kanikowska, Ewelina Swora-Cwynar, Marian Grzymislawski, Maki Sato, Andrzej Breborowicz, Janusz Witowski, Katarzyna Korybalska
Summary: This study investigated the effects of moderate and short-term calorie restriction on obese patients, finding that it partially improves antioxidant capacity and significantly changes anthropometric parameters, accompanied by improvements in important physiological indicators.
Article
Nutrition & Dietetics
Miriam Scicchitano, Cristina Carresi, Saverio Nucera, Stefano Ruga, Jessica Maiuolo, Roberta Macri, Federica Scarano, Francesca Bosco, Rocco Mollace, Antonio Cardamone, Anna Rita Coppoletta, Lorenza Guarnieri, Maria Caterina Zito, Irene Bava, Luca Cariati, Marta Greco, Daniela Patrizia Foti, Ernesto Palma, Micaela Gliozzi, Vincenzo Musolino, Vincenzo Mollace
Summary: Our study showed that Ica pretreatment of H9c2 cells before Doxo exposure improved cell viability, reduced ROS generation, prevented mitochondrial dysfunction and mPTP opening. Additionally, it was found that Ica inhibited Caveolin-1 expression levels and PDE5a activity, enhancing mitochondrial function and preventing apoptotic cell death while regulating autophagic processes.
Review
Nutrition & Dietetics
Emiliana Giacomello, Luana Toniolo
Summary: Aging is a biological process influenced by multiple cellular mechanisms leading to functional decline. The growth of the elderly population and their susceptibility to diseases drive the search for anti-aging interventions, with calorie restriction identified as an effective approach.
Article
Nutrition & Dietetics
Sunil Poudel, Gil Martins, M. Leonor Cancela, Paulo J. J. Gavaia
Summary: Osteoporosis is a disease characterized by abnormal bone structure and low bone mass. Oxidative stress and imbalances in osteoblast and osteoclast activity are believed to be the primary cause of secondary osteoporosis. This study examined the effects of antioxidants Resveratrol and MitoTEMPO on bone impairment induced by the chemotherapy drug Doxorubicin in zebrafish. The results showed that supplementation with antioxidants effectively improved overall growth and mineralization, counteracting the bone anomalies caused by Doxorubicin.
Article
Nutrition & Dietetics
Sunil Poudel, Marisol Izquierdo, Maria Leonor Cancela, Paulo J. Gavaia
Summary: Doxorubicin-induced bone loss can be counteracted by antioxidants such as resveratrol and MitoTEMPO. These antioxidants improve mineralization, decrease bone deformities, and reverse the effects of doxorubicin by increasing antioxidant response. Antioxidant supplements effectively prevent bone abnormalities and mineralization defects caused by doxorubicin, showing potential for preventing doxorubicin-induced bone impairment.
Article
Cell Biology
Dennis Mangan
Summary: Iron is essential for living organisms, but excessive accumulation can lead to health issues, age-related diseases, and shortened lifespan. Blocking iron absorption can extend lifespan, while controlling body iron stores may be an important intervention for health and longevity.
Article
Multidisciplinary Sciences
Nan Wu, Yi-Cheng Ma, Xin-Qian Gong, Pei-Ji Zhao, Yong-Jian Jia, Qiu Zhao, Jia-Hong Duan, Cheng-Gang Zou
Summary: Metabolism is closely related to aging, and endogenous metabolites may delay aging and improve health. The activation of the transsulfuration pathway is linked to peroxisome function and biogenesis in long-lived Caenorhabditis elegans worms. Supplementation with alpha-ketobutyrate, an intermediate of the transsulfuration pathway, extends lifespan in wild-type worms. Alpha-ketobutyrate enhances NAD(+) production and improves peroxisome function and biogenesis through the SIR-2.1/SIRT1 pathway. It also promotes the expression of genes related to autophagy and lysosomes, ultimately extending lifespan. Alpha-ketobutyrate also delays cellular senescence in fibroblast cells. Understanding how metabolites modulate longevity is crucial for reducing aging-related disease.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Chenfei Li, Zhen Guo, Fangyuan Liu, Peng An, Mingyu Wang, Dan Yang, Qizhu Tang
Summary: Doxorubicin (DOX) is a commonly used chemotherapeutic drug in cancer treatment, but its side effects on the heart restrict its clinical application. In this study, researchers found that apoptosis, oxidative stress, and autophagy all play important roles in DOX-induced cardiac injury. They demonstrated that overexpression of PCSK6, a protein involved in cardiovascular diseases, can protect against DOX-induced cardiac damage by promoting autophagy and inhibiting apoptosis and oxidative stress. They also identified the involvement of SIRT1 and FOXO3a in the protective effect of PCSK6.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Geriatrics & Gerontology
Mohammad Murtaza Mehdi, Preeti Solanki, Prabhakar Singh
Summary: Aging is defined as the result of oxidative stress affecting cells, leading to cellular dysfunction and slowing down of the system. Slowing down the aging process involves reducing the rate of cellular oxidative damage, which is related to genetic factors, lifestyle, and diet. Understanding aging theories and molecular damage caused by oxidative stress is crucial for anti-aging strategies.
ARCHIVES OF GERONTOLOGY AND GERIATRICS
(2021)
Article
Biochemistry & Molecular Biology
Sunil Poudel, Gil Martins, M. Leonor Cancela, Paulo J. Gavaia
Summary: The study found that resveratrol (RES) can effectively prevent DOX-induced osteoporosis, both in vitro and in vivo. RES inhibits the adverse effects of DOX in osteoclast fusion and activation, while also improving mucositis and locomotor behavior in a zebrafish model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Margalida Torrens-Mas, Cayetano Navas-Enamorado, Devin Wahl, Andres Sanchez-Polo, Anna Picca, Jordi Oliver, Pilar Roca, Marta Gonzalez-Freire
Summary: “Calorie restriction (CR) is the only nutritional strategy proven to extend lifespan, prevent age-related diseases, and delay functional decline. The effects of CR when started early in life are not well understood. This study found that young female rats treated with CR showed similar expression patterns of key genes/pathways associated with healthy aging compared to old animals treated with CR, while in male rats these effects were reduced.”
Article
Chemistry, Medicinal
Ahmed M. Kabel, Samir A. Salama, Almokhtar A. Adwas, Remon S. Estfanous
Summary: The study found that fraxetin can mitigate the harmful effects of oxidative stress and inflammation on myocardial muscles in a dose-dependent manner, decrease NLRP3 inflammasome, enhance autophagy, and improve apoptotic signaling pathways. Additionally, fraxetin can counteract the echocardiographic, histopathological, immunohistochemical, and electron microscopic changes induced by doxorubicin in cardiomyocytes in a dose-dependent manner.
Article
Biochemistry & Molecular Biology
Rasha Abu-Khudir, Wafaa M. Ibrahim, Mohammed E. Shams, Afrah F. Salama
Summary: The study demonstrated that trehalose has cardioprotective effects against doxorubicin-induced cardiotoxicity, likely mediated through suppression of oxidative stress and autophagy.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jeong-Woo Park, Jihyeon Jeong, Young-Seuk Bae
Summary: Calorie restriction (CR) and autophagy activation have been found to extend the healthspan of organisms by delaying age-related diseases. This study investigated the role of protein kinase CK2 in CR and autophagy and found that CK2's expression was upregulated by CR, leading to the activation of SIRT1 and AMP-activated protein kinase (AMPK). Overexpression of CK2 alpha stimulated autophagy initiation and nucleation markers, and several molecules including SIRT1, AKT, mTOR, AMPK, and FoxO3a were involved in CK2-mediated autophagy. The findings suggest that inhibiting certain microRNAs or using their antisense inhibitors could mimic the effects of CR or induce autophagy.
MOLECULES AND CELLS
(2022)
Article
Engineering, Environmental
You Zhang, Wenhong Fan, Xiaomin Li, Wen-Xiong Wang, Shu Liu
Summary: In this study, nanobubble technology and hydrogen water were combined for the first time to remove reactive oxygen species caused by environmental stress. Hydrogen nanobubbles showed remarkable capability in removing H2O2 and O(2)(center dot- ), and increasing the activity and expression of antioxidant enzymes.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Surgery
Kruti Patel, Tamar S. Polonsky, Melina R. Kibbe, Jack M. Guralnik, Lu Tian, Luigi Ferrucci, Michael H. Criqui, Robert Sufit, Christiaan Leeuwenburgh, Dongxue Zhang, Lihui Zhao, Mary M. McDermott
Summary: Among individuals with PAD, there is significant variation in walking improvement after SET, with shorter baseline 6-minute walk distance associated with greater improvement. However, age, sex, disease severity, and other clinical characteristics do not affect responsiveness to exercise therapy.
JOURNAL OF VASCULAR SURGERY
(2021)
Article
Surgery
Mary M. McDermott, Lu Tian, Michael H. Criqui, Luigi Ferrucci, Michael S. Conte, Lihui Zhao, Lingyu Li, Robert Sufit, Tamar S. Polonsky, Melina R. Kibbe, Philip Greenland, Christiaan Leeuwenburgh, Jack M. Guralnik
Summary: Among people with PAD, improvements of approximately 8 and 20 meters in the 6-minute walk distance represent small and large improvements in walking ability, respectively. People with PAD who reported no change in their ability to walk distances over 1 year simultaneously declined by a mean of 7 meters in the 6-minute walk test.
JOURNAL OF VASCULAR SURGERY
(2021)
Article
Geriatrics & Gerontology
Sara A. Harper, John R. Bassler, Sujitha Peramsetty, Youfeng Yang, Lisa M. Roberts, Devin Drummer, Robert T. Mankowski, Christiaan Leeuwenburgh, Karina Ricart, Rakesh P. Patel, Marcas M. Bamman, Stephen D. Anton, Byron C. Jaeger, Thomas W. Buford
Summary: The study aimed to evaluate the safety and feasibility of combining exercise and resveratrol to treat older adults, showing that this combined treatment may improve skeletal muscle mitochondrial function and mobility-related indices of physical function. A larger trial is warranted to further validate these findings.
EXPERIMENTAL GERONTOLOGY
(2021)
Review
Geriatrics & Gerontology
Stephen D. Anton, Yenisel Cruz-Almeida, Arashdeep Singh, Jordan Alpert, Benjamin Bensadon, Melanie Cabrera, David J. Clark, Natalie C. Ebner, Karyn A. Esser, Roger B. Fillingim, Soamy Montesino Goicolea, Sung Min Han, Henrique Kallas, Alisa Johnson, Christiaan Leeuwenburgh, Andrew C. Liu, Todd M. Manini, Michael Marsiske, Frederick Moore, Peihua Qiu, Robert T. Mankowski, Mamoun Mardini, Christian McLaren, Sanjay Ranka, Parisa Rashidi, Sunil Saini, Kimberly T. Sibille, Shinichi Someya, Stephanie Wohlgemuth, Carolyn Tucker, Rui Xiao, Marco Pahor
EXPERIMENTAL GERONTOLOGY
(2020)
Article
Critical Care Medicine
Dijoia B. Darden, Frederick A. Moore, Scott C. Brakenridge, Eduardo B. Navarro, Stephen D. Anton, Christiaan Leeuwenburgh, Lyle L. Moldawer, Alicia M. Mohr, Philip A. Efron, Robert T. Mankowski
CRITICAL CARE CLINICS
(2021)
Article
Cell Biology
Anna Picca, Sunil K. Saini, Robert T. Mankowski, George Kamenov, Stephen D. Anton, Todd M. Manini, Thomas W. Buford, Stephanie E. Wohlgemuth, Rui Xiao, Riccardo Calvani, Helio Jose Coelho-Junior, Francesco Landi, Roberto Bernabei, David A. Hood, Emanuele Marzetti, Christiaan Leeuwenburgh
Review
Geriatrics & Gerontology
Yun Kyung Jeon, Myung Jun Shin, Sunil Kumar Saini, Carlo Custodero, Monica Aggarwal, Stephen D. Anton, Christiaan Leeuwenburgh, Robert T. Mankowski
Summary: Aging-related changes to cardiovascular and musculoskeletal systems contribute to comorbid conditions, ultimately leading to premature death. Vascular calcification, prevalent in older adults, accelerates muscle loss and functional decline, negatively impacting skeletal muscle perfusion. Further research is needed to understand the impact of cardiovascular aging on skeletal muscle perfusion.
EXPERIMENTAL GERONTOLOGY
(2021)
Article
Nutrition & Dietetics
Sunil K. Saini, Arashdeep Singh, Manisha Saini, Marta Gonzalez-Freire, Christiaan Leeuwenburgh, Stephen D. Anton
Summary: Time-restricted eating (TRE), a form of intermittent fasting, has been shown to provide health benefits by influencing the expression of circulatory miRNAs. This study aimed to examine the effects of a 4-week TRE regimen on global circulatory miRNA in older overweight participants. The results demonstrated that TRE downregulated miRNA, which could inhibit cell growth pathways and activate cell survival pathways, potentially promoting healthy aging.
Article
Cardiac & Cardiovascular Systems
Michael M. Hammond, Bonnie Spring, W. Jack Rejeski, Robert Sufit, Michael H. Criqui, Lu Tian, Lihui Zhao, Shujun Xu, Melina R. Kibbe, Christiaan Leeuwenburgh, Todd Manini, Daniel E. Forman, Diane Treat-Jacobson, Tamar S. Polonsky, Lydia Bazzano, Luigi Ferrucci, Jack Guralnik, Donald M. Lloyd-Jones, Mary M. McDermott
Summary: In individuals with peripheral artery disease, walking exercise inducing ischemic leg symptoms improved walking velocity and Short Physical Performance Battery score. Compared to walking exercise without ischemic symptoms, walking exercise inducing ischemic symptoms had a positive effect on walking velocity and physical performance.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Cell Biology
Anna Picca, Matthew Triolo, Stephanie E. E. Wohlgemuth, Matthew S. S. Martenson, Robert T. T. Mankowski, Stephen D. D. Anton, Emanuele Marzetti, Christiaan Leeuwenburgh, David A. A. Hood
Summary: Altered mitochondrial quality in muscle may contribute to age-related decline in physical function. This study investigated the relationship between autophagy, mitophagy, lysosomal markers and measures of physical performance and lower extremity tissue composition in young and older adults. The results revealed that older adults had smaller muscle volume, lower tissue composition index, and higher protein content of p62 and BNIP3. The expression levels of p62 and BNIP3 were negatively correlated with the tissue composition index and performance on the 5-time sit-to-stand test. These findings suggest that dysfunction in the autophagy/mitophagy-lysosomal system is associated with deterioration of lower extremity tissue composition and muscle dysfunction.
Editorial Material
Cell Biology
Anna Picca, Emanuele Marzetti, Christiaan Leeuwenburgh
Article
Cardiac & Cardiovascular Systems
Luigi Ferrucci, Julian Candia, Ceereena Ubaida-Mohien, Alexey Lyashkov, Nirad Banskota, Christiaan Leeuwenburgh, Stephanie Wohlgemuth, Jack M. Guralnik, Mary Kaileh, Dongxue Zhang, Robert Sufit, Supriyo De, Myriam Gorospe, Rachel Munk, Charlotte A. Peterson, Mary M. McDermott
Summary: Transcriptomic and proteomic analyses revealed activation of hypoxia-compensatory mechanisms, inflammation, fibrosis, apoptosis, angiogenesis, unfolded protein response, and nerve and muscle repair in peripheral artery disease (PAD) muscle. Aberrant proportions of mitochondrial respiratory proteins and reduced activity of rate-limiting glycolytic enzymes were also observed. These mechanisms may serve as potential targets for disease modification.
CIRCULATION RESEARCH
(2023)
Article
Nutrition & Dietetics
Dongyu Zhang, Kori A. Spiropoulos, Akemi Wijayabahu, Demetra D. Christou, Shama D. Karanth, Stephen D. Anton, Christiaan Leeuwenburgh, Muxuan Liang, Meghann Wheeler, Danting Yang, Aduse-Poku Livingstone, Robert T. Mankowski, Ting -Yuan David Cheng, Hanchao Zhang, Erin M. Siegel, Frank J. Penedo, Jonathan D. Licht, Dejana Braithwaite
Summary: This study aimed to investigate the effects of low muscle mass (LMM) on the risk of all-cause and cardiovascular disease (CVD)-specific mortality in cancer survivors. The results showed that LMM was positively associated with the risk of all-cause and CVD-specific mortality in cancer survivors.
Article
Biochemistry & Molecular Biology
Robert T. Mankowski, Stephanie E. Wohlgemuth, Guilherme Bresciani, A. Daniel Martin, George Arnaoutakis, Tomas Martin, Eric Jeng, Leonardo Ferreira, Tiago Machuca, Mindaugas Rackauskas, Ashley J. Smuder, Thomas Beaver, Christiaan Leeuwenburgh, Barbara K. Smith
Summary: Mechanical ventilation during cardiothoracic surgery can lead to ventilator-induced diaphragm dysfunction (VIDD) and delay recovery. In this study, intraoperative phrenic nerve stimulation was performed to offset VIDD and investigate changes in mitochondrial function.
Article
Biochemistry & Molecular Biology
Philip A. Efron, Dijoia B. Darden, Zhongkai Wang, Dina C. Nacionales, Maria-Cecilia Lopez, Russell B. Hawkins, Michael C. Cox, Jaimar C. Rincon, Ricardo Ungaro, Marvin L. Dirain, Gabriela L. Ghita, Tianmeng Chen, Timothy R. Billiar, Matthew J. Delano, Christiaan Leeuwenburgh, Azra Bihorac, Scott C. Brakenridge, Frederick A. Moore, Alicia M. Mohr, Ronald G. Tompkins, Babette A. Brumback, Henry Baker, Gilbert R. Upchurch, Lyle L. Moldawer
Summary: The improved murine model of surgical sepsis more closely approximates human surgical sepsis patients in terms of gene expression, particularly in the more chronic phases of sepsis.
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)