Article
Biochemistry & Molecular Biology
Ashwini Sri Hari, Rajeswari Banerji, Li -Ping Liang, Ruth E. Fulton, Christopher Quoc Huynh, Timothy Fabisiak, Pallavi Bhuyan Mcelroy, James R. Roede, Manisha Patel
Summary: Elevation of glutathione (GSH) levels has been shown to decrease oxidative stress and seizure frequency, as well as rescue cognitive deficits in epilepsy. Treatment with dimercaprol (DMP), which increases cellular GSH, inhibits neuronal hyperexcitability and reduces seizures in vitro and in vivo. The redox-sensitive mTORC1 pathway may be a plausible link between GSH elevation and inhibition of neuronal hyperexcitability.
Article
Biochemistry & Molecular Biology
Wazifa Afrin, Shigeki Furuya, Kohji Yamamoto
Summary: This study identified the presence of glutamate-cysteine ligase (GCL) in silkworms, which is involved in the synthesis of glutathione (GSH) by catalyzing the ligation of glutamate and cysteine using ATP and Mg2+. High levels of bmgcl mRNA and GSH were observed in the silkworm fat body, indicating their potential role in oxidative stress resistance.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Duk-Shin Lee, Ji-Eun Kim
Summary: This study found that P2X7 receptor plays an important role in LPS-induced neuroinflammation by affecting Nrf2 activity/expression and GSH synthesis.
Article
Biochemistry & Molecular Biology
Misaki Okada, Yoshio Kimura
Summary: The production of glutathione (GSH) in Myxococcus xanthus is mainly dependent on the activity of mxGcl2. This enzyme is activated by Mn2+ and stabilized in the presence of Mn2+ or Mg2+. The substitution of amino acid residues in the active site can modulate its affinity for substrates. Additionally, other substances such as GSSG, ATP, buthionine sulfoximine, pyrophosphate, and polyphosphates can affect the activity of mxGcl2.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Lydia H. Zhang, Michelle Tang, Xavier Tao, Qian Shao, Vienna Thomas, Saki Shimizu, Miki Kasano, Yoshinori Ishikawa, Takayuki Inukai, Daniel K. Nomura
Summary: Dysregulated oxidative stress is important in cancer pathogenesis, and certain types of cancer cells are sensitive to inhibition of their antioxidant capacity. In this study, we describe a cysteine-reactive ligand, EN25, that targets an allosteric cysteine C114 on the modifier subunit of GCL, leading to inhibition of GCL activity and reduced cellular GSH levels. This interaction impairs cell viability in ARID1A-deficient cancer cells, which are vulnerable to glutathione depletion. Our findings uncover a potential druggable site within GCLM for inhibiting GSH synthesis in vulnerable cancer cell types.
Article
Endocrinology & Metabolism
Flavia Bittencourt Brasil, Fhelipe Jolner Souza de Almeida, Matheus Dargesso Luckachaki, Evandro Luiz Dall'Oglio, Marcos Roberto de Oliveira
Summary: The reactive dicarbonyl methylglyoxal (MG) induces redox dysfunction and mitochondrial impairments in cells, while isoorientin (ISO) serves as an antioxidant and prevents these damages in human neuroblastoma cells.
METABOLIC BRAIN DISEASE
(2023)
Article
Cell Biology
Roon Banerjee, Aishwarya Raj, Chandrakanta Potdar, Pramod Kumar Pal, Ravi Yadav, Nitish Kamble, Vikram Holla, Indrani Datta
Summary: In this study, we investigated the cell-intrinsic effect of the LRRK2-I1371V mutant variant on astrocyte biology. Our findings reveal that the mutation in the LRRK2-I1371V allele significantly impairs astrocytic function in terms of Nrf2-mediated antioxidant machinery, ATP generation, and glutamate metabolism, despite comparable astrocyte yields.
Article
Oncology
Evan L. Carpenter, Alyssa L. Becker, Arup K. Indra
Summary: Melanocytes are pigment-producing cells in the skin that protect against UV radiation by synthesizing melanin and distributing it to adjacent cells. They face oxidative stress and utilize antioxidant systems, regulated by NRF2, to prevent cancer formation. However, melanomas hijack these systems for uncontrolled growth and metastasis, presenting a challenge for treatment.
Review
Neurosciences
Ilkay Erdogan Orhan
Summary: Natural compound quercetin, particularly its neuroprotective properties, is of interest in the treatment of Alzheimer's Disease. Studies on quercetin's potential as a ChE inhibitor are limited but warrant further investigation for potential therapeutic benefits.
CURRENT NEUROPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Paraskevi-Maria Psefteli, Phoebe Kitscha, Gema Vizcay, Roland Fleck, Sarah J. Chapple, Giovanni E. Mann, Mark Fowler, Richard C. Siow
Summary: The activation of Nrf2 pathway is crucial for maintaining vascular endothelial redox homeostasis under high shear stress. This study reveals the important role of glycocalyx sialic acids (SIA) in regulating Nrf2 signaling in response to shear stress, demonstrating a synergistic relationship between SIA and Nrf2 in preventing atherosclerosis. The findings suggest that targeting the endothelial glycocalyx could be a potential therapeutic approach for cardiovascular disease and redox dysregulation associated with aging.
Article
Cell Biology
Agnieszka Scibior, Iwona Wojda, Ewa Wnuk, Lukasz Pietrzyk, Zbigniew Plewa
Summary: The study reveals that vanadium can induce oxidative stress and affect a range of protective and detoxifying proteins in cells at certain levels. Additionally, there is a synergistic effect between vanadium and magnesium in antioxidant defense.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Article
Biochemistry & Molecular Biology
Jianrui Zhang, Si-an Xie, Jin Wang, Jiayu Liu, Yueqi Liu, Shuang Zhou, Xixi Li, Lili Han, Wei Pang, Weijuan Yao, Yi Fu, Wei Kong, Min Ye, Jing Zhou
Summary: This study demonstrates a correlation between decreased vascular compliance and atherosclerosis, and proposes that softening the arterial wall may protect against the disease. Through a screening process, the compound echinatin was found to reduce arterial wall stiffness. Further investigation showed that echinatin mitigated arterial stiffening and atherosclerosis, possibly through its upregulation of glutamate cysteine ligases and maintenance of glutathione metabolism.
Article
Biochemistry & Molecular Biology
Eunsook S. Jin, Craig R. Malloy, Gaurav Sharma, Erin Finn, Kelly N. Z. Fuller, Yesenia Garcia Reyes, Mark A. Lovell, Sarkis C. Derderian, Jonathan A. Schoen, Thomas H. Inge, Melanie G. Cree
Summary: This study investigates the role of glycerol in liver metabolism, including glutathione. The results show that glycerol can be incorporated into glutathione through glycine or glutamate metabolism, which may represent a compensatory mechanism in the setting of excess glycerol delivery to the liver.
Article
Oncology
Chiou-Hong Lin, John P. Vu, Chen-Yen Yang, Mint Sirisawad, Chun-Te Chen, Hung Dao, Jing Liu, Xuan Ma, Chin Pan, Joseph Cefalu, Chris Tse, Erica Jackson, Hsu-Ping Kuo
Summary: Studies on new therapeutic targets for AML suggest that GCLC is essential for AML cells but dispensable for normal HSPCs, making it a potential therapeutic target for AML.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Marina A. Bykanova, Maria A. Solodilova, Iuliia E. Azarova, Elena Y. Klyosova, Olga Y. Bushueva, Anna A. Polonikova, Mikhail I. Churnosov, Alexey V. Polonikov
Summary: This study found that genetic variation at the GCLC gene may contribute to the risk of colorectal cancer, and certain genotype combinations of the GCLC gene are associated with the risk of CRC. Functional SNP annotation also suggests that the CRC-associated polymorphisms are associated with a decreased GCLC expression.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Entomology
Anais Rodriguez Luis, Carlos Ariel Yadro Garcia, Ciro Invernizzi, Belen Branchiccela, Adolfo Mauricio Perez Pineiro, Alejandro Perez Morfi, Pablo Zunino, Karina Antunez
JOURNAL OF APICULTURAL RESEARCH
(2020)
Article
Ecology
L. Castelli, B. Branchiccela, M. Garrido, C. Invernizzi, M. Porrini, H. Romero, E. Santos, P. Zunino, K. Antunez
Article
Neurosciences
Carolina Echeverry, Giselle Prunell, Camila Narbondo, Veronica Sanchez de Medina, Xavier Nadal, Miguel Reyes-Parada, Cecilia Scorza
Summary: Preclinical studies have shown that CBD and CBG from Cannabis sativa have neuroprotective effects on toxic and neurodegenerative processes. CBD and CBG were found to be more effective against rotenone-induced neurotoxicity, with CBDA also showing some reduction in toxicity. The role of 5-HT1A receptors in the neuroprotective effect of CBG was highlighted in the study.
NEUROTOXICITY RESEARCH
(2021)
Article
Entomology
Belen Branchiccela, Loreley Castelli, Sebastian Diaz-Cetti, Ciro Invernizzi, Yamandu Mendoza, Estela Santos, Carlos Silva, Pablo Zunino, Karina Antunez
Summary: Supplementing honey bee colonies with polyfloral pollen can increase their strength and health when facing nutritional stress. However, mitigating the negative impact of fall nutritional stress during the winter season is difficult.
JOURNAL OF APICULTURAL RESEARCH
(2023)
Article
Ecology
Loreley Castelli, Belen Branchiccela, Hector Romero, Pablo Zunino, Karina Antunez
Summary: Honey bees play a crucial role in food production and biodiversity maintenance through pollination. They have different survival strategies in cold temperate regions and experience lifecycle alterations in tropical or subtropical climates. The gut microbiota of honey bees in subtropical colonies changes seasonally, with environmental variables impacting its composition.
Article
Microbiology
Loreley Castelli, Sofia Balbuena, Belen Branchiccela, Pablo Zunino, Joanito Liberti, Philipp Engel, Karina Antunez
Summary: Glyphosate has been shown to have detrimental effects on honey bees, altering their gut microbiota, immune response, and promoting virus replication. Increased immune gene expression may represent a physiological response, but is insufficient to maintain bee health. Overall, the widespread use of glyphosate contributes to a global decline in pollination services.
Article
Microbiology
Loreley Castelli, Maria Laura Genchi Garcia, Anne Dalmon, Daniela Arredondo, Karina Antunez, Ciro Invernizzi, Francisco Jose Reynaldi, Yves Le Conte, Alexis Beaurepaire
Summary: RNA viruses, particularly DWV, play a significant role in pollinator losses. This study analyzed DWV variants in Varroa destructor-parasitized pupae, finding that only DWV was detected and its two variants showed significant differences across sampling periods, colonies, and patrilines.
Article
Biochemistry & Molecular Biology
V. Cardozo, L. Vaamonde, A. Parodi-Talice, M. J. Zuluaga, D. Agrati, M. Portela, A. Lima, F. Blasina, F. Dajas, G. Bedo
Summary: Hypoxic-ischemic encephalopathy (HIE) leads to mortality and long-term neurologic morbidities in newborns, involving energy failure, excitotoxicity, and oxidative stress. Quercetin has been shown to have neuroprotective effects in perinatal asphyxia models, reducing mortality and affecting protein expression. The glycolytic pathway is strongly regulated by quercetin in both rat and piglet models, indicating potential neuroprotective mechanisms.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Gonzalo Ferreira, Fernanda Blasina, Marianela Rodriguez Rey, Gabriel Anesetti, Rosana Sapiro, Luisina Chavarria, Romina Cardozo, Grazzia Rey, Luis Sobrevia, Garth L. Nicolson
Summary: During pregnancy, physiological changes make the mother and fetus more susceptible to certain infections, which can lead to severe diseases. Pregnant women with COVID-19 have an increased risk of hospitalization but not for intensive care or mechanical ventilation compared to non-pregnant women. Neonates have a low risk of acquiring COVID-19 but may be more at risk for severe disease compared to older children.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Ecology
Sofia Balbuena, Loreley Castelli, Pablo Zunino, Karina Antunez
Summary: Large-scale honey bee colony losses around the world are attributed to pesticide intoxication and the presence of pests and pathogens. Among pesticides, neonicotinoid insecticides pose the greatest threat. This study investigated the effects of chronic exposure to imidacloprid and Nosema ceranae on Africanized honey bees, and found altered immunity and high mortality rates. The study highlights the negative impact of these stress factors on honey bee colonies in Latin America.
Article
Entomology
Daniela Arredondo, Pablo Zunino, Karina Antunez
Summary: The administration of probiotics can improve human and animal health. In a previous study, researchers developed a mixture of Apilactobacillus kunkeei strains that reduced infection and mortality in honey bees. However, the effectiveness of probiotic treatments can vary, so monitoring their administration is important.
JOURNAL OF APICULTURAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Carolina Echeverry, Analia Richeri, Jimena Fagetti, Gaby F. Martinez, Federico Vignolo, Giselle Prunell, Leticia Cunetti, Marcela Martinez Busi, Sandra Perez, Veronica Sanchez de Medina, Carlos Ferreiro, Cecilia Scorza
Summary: This study investigated the neuroprotective activity of a pharmaceutical cannabis-based extract, Epifractan (EPI), on cerebellar granule cells. The results showed that EPI significantly reduced neurotoxicity without causing any toxicity itself, indicating its potential for neuroprotection in neurodegenerative diseases.
CANNABIS AND CANNABINOID RESEARCH
(2023)
Article
Agronomy
A. Mondino, K. Yaneselli, A. Ingold, C. Echeverry, S. Raffaelli, A. Vazquez, C. Garcia y Santos
Summary: This study aimed to evaluate the cytotoxic activity of a low PA extract of S. madagascariensis on cancer-derived cell lines. The results demonstrated that the extract showed cytotoxic activity on the cancer cells, and the degree of cytotoxicity depended on the concentration of the solution.
AGROCIENCIA URUGUAY
(2022)
Article
Biology
F. Silvera, T. Gagliardi, P. Vollono, C. Fernandez, A. Garcia-Bayce, A. Berardi, M. Badia, B. Beltran, T. Cabral, P. Abella, L. Farias, L. Vaamonde, M. Martell, F. Blasina
Summary: This study investigated the efficacy of using NIRS to detect acute changes in cerebral blood flow following PaCO2 fluctuations in newborn piglets, finding significant correlations between cerebral oxygenation, Doppler-derived parameters of blood flow, and PaCO2 levels. The results showed that cerebral blood flow varied significantly under different ventilatory settings, demonstrating the potential of NIRS in monitoring cerebral circulation during ventilation.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2022)
Review
Rheumatology
A. Hoxha, E. Mattia, A. Zanetti, G. Carrara, N. Morel, N. Costedoat-Chalumeau, A. L. Brucato, A. Ruffatti
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
(2020)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)