Article
Immunology
Xin Wang, Jia-ying Yu, Yan Sun, Heng Wang, Hu Shan, Shubai Wang
Summary: Baicalin, a bioactive extract from Scutellaria Baicalensis Georgi, has anti-inflammatory and anti-oxidative functions, and its preventive protection can improve cell apoptosis and Fluorescein sodium infiltration in the brain tissue. Baicalin can also inhibit pro-inflammatory cytokines production, up-regulate Claudin-5 and ZO-1 protein expression, reduce ROS and MDA production, promote SOD production, and activate Nrf2-mediated antioxidant stress pathway to protect blood-brain barrier damage.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Review
Cell Biology
Nachiket M. Godbole, Asif Alam Chowdhury, Neha Chataut, Shanjana Awasthi
Summary: The lung epithelium plays a crucial role in responding to infections, toxins, and allergies, as well as maintaining normal pulmonary function. The tight junctions between lung epithelial cells are essential for maintaining the integrity and fluid balance of the lung. This review summarizes the literature on disrupted tight junctions and epithelial barrier in various lung conditions, and discusses the expression and regulation of specific tight junction proteins against pathogenic stimuli. The review also explores the molecular signaling and crosstalk among signaling pathways that control the tight junction structure and function in the lung.
Article
Gastroenterology & Hepatology
Luz del Carmen Martinez-Sanchez, Phuong Anh Ngo, Rashmita Pradhan, Lukas-Sebastian Becker, David Boehringer, Despina Soteriou, Marketa Kubankova, Christine Schweitzer, Tatyana Koch, Veronika Thonn, Lena Erkert, Iris Stolzer, Claudia Guenther, Christoph Becker, Benno Weigmann, Monika Klewer, Christoph Daniel, Kerstin Amann, Stefan Tenzer, Raja Atreya, Martin Bergo, Cord Brakebusch, Alastair J. M. Watson, Jochen Guck, Ben Fabry, Imke Atreya, Markus F. Neurath, Rocio Lopez-Posadas
Summary: Abnormal cell shedding is associated with intestinal barrier dysfunction and inflammatory bowel diseases. Deletion of PGGTase and RAC1 in intestinal epithelial cells leads to cell overcrowding and epithelial leakage, resulting in chronic intestinal inflammation. RAC1 plays a crucial role in regulating cytoskeletal dynamics, cell mechanics, and intestinal cell shedding.
Article
Multidisciplinary Sciences
Henrik Dimke, Camille Griveau, Wung-Man Evelyne Ling, Gaelle Brideau, Lydie Cheval, Pravina Muthan, Dominik Mueller, Amr Al-Shebel, Pascal Houillier, Caroline Prot-Bertoye
Summary: The kidney plays a critical role in maintaining mineral balance in the body. Dysregulation of calcium and magnesium reabsorption in the renal tubules can lead to familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC), which is characterized by excessive wasting of calcium and magnesium. This study investigated the localization of claudin-19 (CLDN19), a protein involved in mineral reabsorption, in the kidneys and found that it is primarily located in the basolateral membrane of the renal tubules. Additionally, the study showed that CLDN19 interacts with claudin-16 (CLDN16) to regulate permeability in the tubules. These findings provide insights into the pathophysiology of FHHNC and highlight the importance of CLDN19 in maintaining mineral homeostasis in the kidney.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Juanshan Zheng, Anum Ali Ahmad, Yayuan Yang, Zeyi Liang, Wenxiang Shen, Min Feng, Jiahao Shen, Xianyong Lan, Xuezhi Ding
Summary: In this study, Lactobacillus rhamnosus CY12 was found to alleviate oxidative stress, inflammation, and disruption of tight junction barrier functions induced by lipopolysaccharide. The protective effects were mediated through modulation of antioxidant enzyme gene expression, as well as mRNA and protein expressions of tight junction proteins, and inhibition of the TLR4/NF-κB signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Gastroenterology & Hepatology
Zachary M. Slifer, B. Radha Krishnan, Jay Madan, Anthony T. Blikslager
Summary: Larazotide acetate (LA) is a peptide with eight amino acids that acts as a tight junction regulator to restore intestinal barrier function, currently undergoing phase III clinical trials for celiac disease patients. It is believed to work by reducing zonulin-induced barrier permeability increase and is associated with actin filaments tension.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2021)
Article
Veterinary Sciences
Ruibiao Wang, Yuheng Zhang, Jingbo Zhen, Jinpeng Zhang, Zixuan Pang, Xuewei Song, Lihao Lin, Feng Sun, Yixin Lu
Summary: This study explored the effect of exosomes derived from Trichinella spiralis infective larvae (TsExos) on the barrier function of porcine small intestinal epithelial cells (IPEC-J2). The results showed that TsExos ingestion decreased cell viability, increased FITC-dextran content, lactate dehydrogenase and reactive oxygen species levels. TsExos also induced apoptosis, nuclear pyknosis, and nuclear rupture. In addition, TsExos disrupted tight junctions and altered gene expression.
VETERINARY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Kana Yokoo, Yoshinari Yamamoto, Takuya Suzuki
Summary: Ammonia impairs TJ barrier in Caco-2 cells by increasing oxidative stress, leading to mitochondrial dysfunction, which may precede the ammonia-induced oxidative stress.
Article
Food Science & Technology
Yafei Xu, Yunxiao Xie, Zhengchang Wu, Haifei Wang, Zhenhai Chen, Jingneng Wang, Wenbin Bao
Summary: This study investigated the protective effects of melatonin (MEL) against deoxynivalenol (DON)-induced toxicity in porcine jejunum epithelial cells (IPEC-J2). The results showed that pretreatment with MEL significantly increased cell proliferation, decreased apoptosis and oxidative stress, and attenuated the inflammatory response caused by DON exposure. RNA-seq analysis revealed that MEL protects IPEC-J2 cells from DON toxicity by regulating the expression of genes related to tight junctions and autophagy pathways. Additionally, MEL partly prevented DON-induced disruption of intestinal barrier function and decreased autophagy by activating the AKT/mTOR pathway.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Physiology
Caroline Prot-Bertoye, Camille Griveau, Karsten Skjodt, Lydie Cheval, Gaelle Brideau, Loic Lievre, Elsa Ferriere, Floriane Arbaretaz, Kevin Garbin, Reza Zamani, Niels Marcussen, Lucile Figueres, Tilman Breiderhoff, Dominik Muller, Patrick Bruneval, Pascal Houillier, Henrik Dimke
Summary: This study investigated the distribution of claudin 10, 16, and 19 in the human, mouse, and rat kidney using immunohistochemistry and immunofluorescence. The findings revealed distinct junctional pathways in both human and rodent kidneys, indicating the existence of different permeation profiles in all species investigated.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Atul K. Srivastava, Bharat Somireddy Venkata, Yan Y. Sweat, Heather R. Rizzo, Lea Jean-Francois, Li Zuo, Kathleen W. Kurgan, Patrick Moore, Nitesh Shashikanth, Izabela Smok, Joseph R. Sachleben, Jerrold R. Turner, Stephen C. Meredith
Summary: This study investigates the effects of S408 phosphorylation on occludin protein structure and demonstrates its role in regulating intercellular junction function through interactions with other components.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Yifan Wang, Jiaqi Zhang, Beihua Zhang, Mengxiong Lu, Jing Ma, Zhihong Liu, Jinke Huang, Jinxin Ma, Xuefei Yang, Fengyun Wang, Xudong Tang
Summary: Through experiments, it was found that a drug called MGQD can alleviate ulcerative colitis, and its mechanism of action may be related to the inhibition of inflammation, alleviation of oxidative stress, and repair of intestinal barrier damage.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Ophthalmology
Yun Zhang, Jin-Miao Li, Rong Lu, Zhao Liu, Xin Chen, Cintia S. de Paiva, Stephen C. Pflugfelder, De-Quan Li
Summary: This study demonstrates that the anti-inflammatory cytokine IL-37 plays a novel role in preventing corneal epithelial barrier disruption in dry eye disease by suppressing TNF-alpha and CTSS expression.
Review
Immunology
Xiaopeng Tang, Kangning Xiong, Rejun Fang, Meijun Li
Summary: Weaning is a critical period in pig production, but it often causes stress for piglets due to separation from the sow, changes in diet and living environment, and social challenges. This stress leads to changes in the piglets' small intestine, disrupting digestion and absorption, damaging intestinal barrier function, and resulting in reduced feed intake, increased diarrhea rate, and growth retardation. Understanding the effects of weaning stress on intestinal health is important for developing nutritional strategies to alleviate weaning stress.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Nina A. Hering, Julia Luettig, Britta Jebautzke, Jorg D. Schulzke, Rita Rosenthal
Summary: The study found that ellagic acid and urolithin A affect intestinal barrier function in distinct ways, with ellagic acid acting preventively by strengthening the barrier per se and urolithin A protecting against inflammation-induced barrier dysfunction.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Oncology
Maria E. Cuevas, Jenna M. Gaska, Andrea C. Gist, Jonathan M. King, Rebecca A. Sheller, Maria C. Todd
INTERNATIONAL JOURNAL OF ONCOLOGY
(2015)
Article
Oncology
Maria C. Todd, Heather M. Petty, Jonathan M. King, Brytanie N. Piana Marshall, Rebecca A. Sheller, Maria E. Cuevas
Article
Oncology
Heather E. Rizzo, Elia N. Escaname, Nicholas B. Alana, Elizabeth Lavender, Jonathan Gelfond, Roman Fernandez, Matthew A. Hibbs, Jonathan M. King, Nicholas R. Carr, Cynthia L. Blanco
CLINICAL EPIGENETICS
(2020)
Article
Hematology
Zachary Weber, Ashley Sam, Alejandra Pena, Cody Henderson, Donald McCurnin, Utpal Bhalala, Roger Garcia, Jonathan King, Nicholas Carr
Summary: Serum ferritin levels are elevated in pediatric patients undergoing ECMO, but are not reliable for diagnosing iron overload. Ferritin shows poor correlation with serum iron levels, but correlates well with IL-6 and erythropoietin. Further investigation into the relationship between ferritin and erythropoietin is necessary.
BLOOD CELLS MOLECULES AND DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Ekaterina Vasileva, Domenica Spadaro, Florian Rouaud, Jonathan M. King, Arielle Flinois, Jimit Shah, Sophie Sluysmans, Isabelle Mean, Lionel Jond, Jerrold R. Turner, Sandra Citi
Summary: ZO-1, the major scaffolding protein of tight junctions, recruits cingulin (CGN) and paracingulin (CGNL1) to tight junctions by binding to their N-terminal ZO-1 interaction motif. The binding of CGN to the C-terminal ZU5 domain of ZO-1 promotes ZO-1 stabilization and accumulation at tight junctions by promoting its extended conformation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Amanda K. Leone, Jennifer A. Chun, Christopher L. Koehler, Jonathan Caranto, Jonathan M. King
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2007)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)