Article
Biochemical Research Methods
Kacey D. Cliburn, Marilyn A. Huestis, Jarrad R. Wagner, Philip M. Kemp
Summary: A liquid chromatography-tandem mass spectrometry method was developed and validated for quantifying cannabinoids in postmortem specimens, showing accuracy and stability in measuring various cannabinoids.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Environmental Sciences
Pawel Szpot, Olga Wachelko, Marcin Zawadzki
Summary: The study aimed to establish a method for determining methotrexate in postmortem samples and quantifying its distribution in a fatal intoxication case. The method was found to be simple, accurate, and selective, allowing for routine determination of methotrexate concentrations in postmortem specimens.
Review
Medical Laboratory Technology
Philippe Massonnet, Elodie Grifnee, Jordi Farre-Segura, Justine Demeuse, Loreen Huyghebaert, Thomas Dubrowski, Patrice Dufour, Matthieu Schoumacher, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: Immunocapture is a mature method for sample preparation in quantitation of peptides and proteins. This review provides an overview of clinical applications of immunocapture methods, including protocols with and without enzymatic digestion. The advantages and limitations of these approaches are discussed, along with challenges related to mass spectrometer selection. The potential of these techniques in proteomics-based clinical medicine is demonstrated, paving the way for personalized medicine.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medicine, Legal
Yotvarit Charunyanont, Apinya Tubtimrattana, Nat Tansrisawad, Parath Thirati
Summary: This study examined the effect of body refrigeration during body storage on postmortem formation of GHB in blood samples. The results showed that further formation of GHB is not expected once the plateau of postmortem GHB formation is reached in a refrigerated body. Early body refrigeration can minimize postmortem GHB production.
FORENSIC SCIENCE INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Thomai Mouskeftara, Christina Virgiliou, Achilleas Iakovakis, Nikolaos Raikos, Helen G. Gika
Summary: Pesticide poisoning is a common occurrence due to their high toxicity, especially from organophosphates, carbamates and neonicotinoids. A fast and reliable method for determining these compounds in blood and urine was developed and proven to be accurate, robust and sensitive.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Immunology
Hai Yang, Zhen Huang, Jiali Yue, Jianqi Chen, Mingming Yu, Chengming Qu
Summary: Metabolomic analysis showed that the combination therapy of meropenem/amikacin significantly altered relevant metabolites involved in bacterial cell membrane and cell wall synthesis, as well as interfered with multiple metabolic pathways essential for the survival of P. aeruginosa.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xucong Huang, Zhenghua Wu, Yanchao Liu, Mengqi Jia, Wenjuan Zhao, Shuowen Wang, Xinhui Jiang, Yuefen Lou, Guorong Fan
Summary: A simple, sensitive, and rapid LC-MS/MS method was developed for the simultaneous determination of BIC, M7, and M8 active metabolites in SD rat plasma. The method showed excellent linearity and accuracy for quantitation in plasma samples. Pharmacokinetic analysis revealed improved parameters in the BIC-NPs group compared to the BIC group, with a significant increase in AUC(0-t) for BIC and a double peak phenomenon observed for M7.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Plant Sciences
Yonggui Wang, Jing Xia, Zile Wang, Zhiping Ying, Zhi Xiong, Changming Wang, Rui Shi
Summary: This study investigated the regulatory mechanisms of color and aroma formation during Macadamia integrifolia flower development through transcriptome and metabolome analysis. The results indicated that plant hormone signal transduction, starch sucrose metabolism, phenylpropanoid metabolism, flavonoid biosynthesis, and anthocyanin biosynthesis pathways were related to the development and color formation of Macadamia integrifolia flowers. These findings provide a foundation for improving the quality and yield of Macadamia integrifolia nuts.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Food Science & Technology
Yujihn Kwon, Yongeui Koo, Yoonhwa Jeong
Summary: This study developed an analytical method to determine pyrrolizidine alkaloids in teas using liquid chromatography-tandem mass spectrometry combined with rapid-easy extraction. The method showed excellent repeatability, sensitivity, and reproducibility, and could be applied to various tea samples. Overall, the method allows complete separation of lycopsamine and intermedine peaks, making it useful for monitoring pyrrolizidine alkaloids in teas.
Article
Horticulture
Eugenio Butelli, Katharina Bulling, Lionel Hill, Cathie Martin
Summary: The range of flower and fruit colors is determined by the types of anthocyanins produced, with the degree of hydroxylation on the B-ring affecting the hue of these pigments. By genetically engineering regulatory genes and a biosynthetic gene in tomatoes, different levels of hydroxylation of anthocyanins can be generated, providing unique material for studies.
Article
Biochemistry & Molecular Biology
Lukasz Chrobok, Sylwia Bajkacz, Jasmin Daniela Klich, Marian Henryk Lewandowski
Summary: Orexins are neuropeptides synthesised mainly in the brain lateral hypothalamic area, playing a vital role in feeding behaviour, regulation of the sleep-wake cycle and circadian rhythms. This study developed a LC-MS/MS method for quantitative bioanalysis of orexin in the rat vitreous body, showing potential implications on circadian regulation of vision with the first demonstration of daily rhythm of orexin synthesis by the retina.
Article
Chemistry, Applied
Dandan Sun, Yue Jin, Qingyu Zhao, Chaohua Tang, Yi Li, Hao Wang, Yuchang Qin, Junmin Zhang
Summary: A simplified pre-treatment method was developed for analyzing the folate content in eggs, showing that the main form of folate in egg yolks is 5-methyltetrahydrofolate. Laying hens efficiently converted folic acid in feed into 5-methyltetrahydrofolate in eggs with minimal folic acid deposition.
Article
Engineering, Environmental
Ai Jia, Yingbo C. Guo, George D. Di Giovanni, Matthew D. Prescott, Wei L. Li, Eduardo A. Garcia
Summary: This study explored an innovative EC-EQ approach to improve the interpretation and comparison of ELISA and LC-MS/MS results, providing more accurate cyanotoxin monitoring results for water utilities.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Medicine, Legal
Karen Rygaard, Marie Katrine Klose Nielsen, Kristian Linnet, Jytte Banner, Sys Stybe Johansen
Summary: Hair analysis provides valuable information for forensic investigations on previous drug intake and use patterns. This study determined the concentrations of citalopram, escitalopram, and their metabolites in the hair of deceased individuals with mental illness. The hair concentrations were compared to estimated daily doses, and a positive correlation was found between citalopram concentration in the proximal segment of the hair and blood concentrations. The study contributes valuable information to forensic investigations.
FORENSIC SCIENCE INTERNATIONAL
(2022)
Article
Environmental Sciences
Paulo Henrique Prado Stefano, Ari Roisenberg, Matheus Rossi Santos, Mariana Amaral Dias, Cassiana Carolina Montagner
Summary: In recent decades, changes in human behavior and new technologies have introduced thousands of new compounds called contaminants of emerging concern (CEC) into the environment. These CEC compounds have been detected in various environmental compartments such as soil, surface water, air, and groundwater. This study used statistical analysis by self-organizing maps (SOM) to evaluate the trends of CEC in urban groundwater systems, and found that atrazine, hexazinone, simazine, and other compounds were frequently detected in groundwater samples. Caffeine was also commonly detected in the samples, likely due to a local population habit of consuming a hot drink made of yerba mate.
Article
Medicine, Legal
Heitor S. D. Correa, Ivano Alessandri, Andrea Verzeletti
Summary: This research assessed the usefulness of Raman spectroscopy and gas chromatography-mass spectrometry in analyzing bones. The techniques were found to be useful in molecular taphonomy studies and forensic genetics.
FORENSIC SCIENCE INTERNATIONAL
(2024)