Article
Infectious Diseases
Jacqueline Findlay, Roberto Sierra, Otavio Hallal Ferreira Raro, Marta Aires-de-Sousa, Diego O. Andrey, Patrice Nordmann
Summary: This study analysed a collection of E. coli strains harbouring acquired fosA genes and found high-level resistance to fosfomycin. The strains also carried other antibiotic resistance genes and were capable of transmission through conjugation.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Abdolrahman Khezri, Ekaterina Avershina, Rafi Ahmad
Summary: This study analyzed strains from Norwegian patients and found the IncFIB plasmid to be the most prevalent in both E. coli and K. pneumoniae isolates, along with identifying certain plasmid-mediated antibiotic resistance genes. Hybrid assembly was used to more confidently locate plasmids and predict antibiotic resistance genes.
Article
Microbiology
Shumei Huang, Shuang Wang, Yan Li, Ming Fang, Zengqiang Kou, Baoli Chen, Liuchen Xu, Zhenwang Bi, Hao Xu, Xiaohui Chi, Zhenqiang Bi
Summary: This study found that colistin-resistant Escherichia coli, most of which are multi-drug resistant strains, is common and highly transmissible among healthy residents in rural areas in China. Interventions should be implemented to prevent the spread of colistin-resistant Escherichia coli through health education and tighter regulation of antibiotics.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Microbiology
Racha Beyrouthy, Carolina Sabenca, Frederic Robin, Patricia Poeta, Giberto Igrejas, Richard Bonnet
Summary: This study investigated the spread of Escherichia coli containing the resistance gene bla(CTX-M-1) in different habitats and wild fauna using a genomic approach. The results indicated that CTX-M-1 E. coli were preferentially associated with specific ecosystems, and the IncI1-ST3-bla(CTX-M-1) plasmids were disseminated among all E. coli phylogroups with main divergences located in a recombination region containing bla(CTX-M-1).
Article
Multidisciplinary Sciences
Sirirat Luk-in, Tanittha Chatsuwan, Naris Kueakulpattana, Ubolrat Rirerm, Dhammika Leshan Wannigama, Rongpong Plongla, Ratana Lawung, Chaiwat Pulsrikarn, Siriporn Chantaroj, Pattharaporn Chaichana, Nattida Saksaengsopa, Teerarat Shanthachol, Malee Techapornroong, Sunee Chayangsu, Wanla Kulwichit
Summary: This study reported a high rate of mcr-carrying Salmonella clinical isolates in Thailand, associated with mcr-3 gene and bla(CTX-M-55) co-existence in S. Choleraesuis for the first time, leading to clinical concern. The study highlights the potential reservoirs and vectors in the dissemination of mcr and the co-selection by colistin and/or cephalosporins.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Jose Manuel Ortiz de la Rosa, Patrice Nordmann, Laurent Poirel
Summary: This study reveals that acquisition of antibiotic resistance in Escherichia coli is mainly driven by plasmid acquisition, and exposure to orally given antibiotics can increase the conjugation rates of different types of plasmids encoding broad-spectrum beta-lactamases. Antioxidant molecules may antagonize the ability of antibiotics to increase plasmid conjugation rates, providing a potential strategy to mitigate antibiotic resistance dissemination.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Toyotaka Sato, Shin-ichi Yokota, Tooru Tachibana, Satoshi Tamai, Shigeki Maetani, Yutaka Tamura, Motohiro Horiuchi
Summary: The study found that companion animals can carry fluoroquinolone-resistant Escherichia coli, which may be associated with human clinical infections and can potentially be transmitted to their owners.
Article
Agriculture, Dairy & Animal Science
Toshiyuki Murase, Patchara Phuektes, Hiroichi Ozaki, Sunpetch Angkititrakul
Summary: Plasmid-mediated quinolone resistance (PMQR) was found to be prevalent in Escherichia coli in chicken farms, as detected in chicken feces, carcass surface samples, and retail chicken meat samples. The isolates showed varying levels of resistance to different antimicrobials. It was observed that the qnrS gene is transferred horizontally through plasmids and can be co-selected with the use of TMP.
Article
Infectious Diseases
Sifuna Anthony Wawire, Oleg N. Reva, Thomas J. O'Brien, Wendy Figueroa, Victor Dinda, William A. Shivoga, Martin Welch
Summary: This study used whole genome sequencing to investigate antimicrobial resistance and virulence-associated genes in clinical and municipal wastewater Escherichia coli isolates from Kakamega, west Kenya. It found that clinical isolates had more genes required for in-host survival, while plasmids from clinical isolates were enriched in AMR and VA genes, suggesting plasmids as a primary means for acquisition and dissemination of these genes among strains.
INFECTION GENETICS AND EVOLUTION
(2021)
Review
Environmental Sciences
Shaqiu Zhang, Muhammad Abbas, Mujeeb Ur Rehman, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Dekang Zhu, Xinxin Zhao, Qun Gao, Bin Tian, Anchun Cheng
Summary: Colistin drug resistance is an emerging public health threat worldwide, with prevalence in multiple reservoirs and ecological environmental settings. Escherichia coli carrying mobile colistin resistance genes exhibit abundant clinically important resistance genes and are spread through various plasmid replicon types.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Infectious Diseases
Teresa Grilo, Samanta Freire, Bruno Miguel, Luis Nogueira Martins, Maria Favila Menezes, Patrice Nordmann, Laurent Poirel, Maria Jose Rego Sousa, Marta Aires-de-Sousa
Summary: This study evaluated the occurrence of plasmid-mediated fos genes among fosfomycin-resistant Escherichia coli isolates collected from patients in Lisbon, Portugal. The results showed a low prevalence of fosfomycin-resistant E. coli isolates, but 15% of these isolates carried transferable fosA genes, and a high rate of extended-spectrum beta-lactamase (ESBL) production was observed.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Kwangwon Seo, Kyung-Hyo Do, Wan-Kyu Lee
Summary: This study investigated the molecular characteristics of fluoroquinolone-resistant E. coli isolates from suckling piglets with colibacillosis. The results showed that all isolates exhibited multi-drug resistance and mutations in quinolone resistance determining regions. Plasmid-mediated quinolone resistance genes were also identified, along with other resistance genes and toxin genes.
Article
Biotechnology & Applied Microbiology
Tomas Nohejl, Jana Palkovicova, Kristina Nesporova, Adam Valcek, Jarmila Lausova, Monika Dolejska
Summary: The fos operon enables bacteria to thrive in challenging environments, and its global distribution in bacterial genomes is still unknown. The presence of plasmid-harbored fos operons in resistant bacteria may contribute to the spread of antibiotic resistance.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Guilhem Royer, Jose-Manuel Ortiz de La Rosa, Xavier Vuillemin, Beatrice Lacombe, Francoise Chau, Olivier Clermont, Melanie Mercier-Darty, Jean-Winoc Decousser, Jean-Damien Ricard, Patrice Nordmann, Erick Denamur, Laurent Poirel
Summary: Chlorhexidine is a widely used antiseptic, but there is a growing concern about decreased susceptibility to this compound in bacteria. This study investigated the resistance mechanisms of Escherichia coli to chlorhexidine through whole-genome sequence analysis. The results showed that two different evolutionary paths lead to chlorhexidine decreased susceptibility, one involving a retrograde phospholipid transport system and the other associated with efflux pump TetA.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Yan Li, Yang Sun, Shi-wen Sun, Bing Liang, Bo-wen Jiang, Na Feng, Jun Liu, Xue Ji
Summary: This study aimed to investigate the antimicrobial resistance and virulence genes of Klebsiella pneumoniae and Raoultella strains isolated from captive giant pandas. Fecal samples were collected from 128 giant pandas and microbial strains were tested for drug susceptibility and gene detection. The results showed high antibiotic resistance rates and multidrug-resistant strains, including the first reported multidrug-resistant R. ornithinolytica strain in captive giant pandas. Various resistance and virulence genes were detected, indicating potential risks to the pandas and the keepers. Regular monitoring of antibiotic resistance and virulence genes in Klebsiella and Raoultella is recommended for panda conservation.