Article
Multidisciplinary Sciences
Xiaofang Wu, Lei Ji, Yuehua Shen, Liping Chen, Deshun Xu, Fenfen Dong
Summary: Thirteen pathogenic Campylobacter strains were isolated from diarrhea cases and raw poultry meat products in Huzhou, including 11 Campylobacter jejuni and 2 Campylobacter coli strains. These strains contain multiple virulence-related genes and show high resistance to nalidixic acid, ciprofloxacin, and tetracycline. Genomic sequencing revealed the presence of tet(O) and blaOXA resistance genes in all C. jejuni strains and multiple drug resistance genes in 2 C. coli strains.
Article
Immunology
Craig T. Parker, Kerry K. Cooper, Francesca Schiaffino, William G. Miller, Steven Huynh, Hannah K. Gray, Maribel Paredes Olortegui, Paul Garcia Bardales, Dixner Rengifo Trigoso, Pablo Penataro-Yori, Margaret N. Kosek
Summary: Campylobacter jejuni is the leading bacterial cause of gastroenteritis worldwide, and this study isolated and sequenced two C. jejuni strains from guinea pigs in the Peruvian Amazon, revealing unique genomic features that may be associated with guinea pig host specialization.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Immunology
Se-Hun Kim, Ramachandran Chelliah, Sudha Rani Ramakrishnan, Ayyappasamy Sudalaiyadum Perumal, Woo-Suk Bang, Momna Rubab, Eric Banan-Mwine Daliri, Kaliyan Barathikannan, Fazle Elahi, Eunji Park, Hyeon Yeong Jo, Su-Bin Hwang, Deog Hwan Oh
Summary: Campylobacter spp. are the leading cause of bacterial colon infections in humans globally, and their stress-adaptation mechanisms differ from other enteropathogenic bacteria, allowing them to survive in diverse environments and enhance their resistance mechanisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Environmental Sciences
Shuxin Zhang, Jiahua Shi, Xuan Li, Lachlan Coin, Jake W. O'Brien, Muttucumaru Sivakumar, Faisal Hai, Guangming Jiang
Summary: This study developed a triplex qPCR assay for the detection and quantification of Campylobacter species in wastewater samples, specifically targeting C. jejuni and C. coli. The assay demonstrated high sensitivity and reliability, and can be used for long-term monitoring of Campylobacter prevalence in communities and surrounding environments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Ruby Costigan, Emily Stoakes, R. Andres Floto, Julian Parkhill, Andrew J. Grant
Summary: This study reports the development of a CRISPRi-based tool in C. jejuni, which will be a valuable resource for the Campylobacter community. The tool was successfully validated through the repression of target genes and resulted in phenotypical changes.
Article
Multidisciplinary Sciences
Nikola Ondrikova, Helen E. Clough, Amy Douglas, Miren Iturriza-Gomara, Lesley Larkin, Roberto Vivancos, John P. Harris, Nigel A. Cunliffe
Summary: The COVID-19 pandemic had a more adverse impact on laboratory reporting of norovirus compared to Campylobacter in England. This may be explained by stronger behavioral interventions affecting norovirus transmission and a greater reduction in norovirus testing capacity. The study highlights the differential impact a pandemic can have on surveillance of gastrointestinal infectious diseases.
Article
Biochemistry & Molecular Biology
Fabia D. Lobo de Sa, Steffen Backert, Praveen K. Nattramilarasu, Soraya Mousavi, Geoffrey Sandle, Stefan Bereswill, Markus M. Heimesaat, Jorg-Dieter Schulzke, Roland Buecker
Summary: The study found that Vitamin D can improve the intestinal barrier dysfunction caused by Campylobacter infection, reducing bacterial transmigration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Annika Dreyer, Christof Lenz, Uwe Gross, Wolfgang Bohne, Andreas Erich Zautner
Summary: In this study, the influence of co-incubation with different bacterial species on the proteome of C. jejuni was investigated using mass spectrometry. The results showed that co-incubation with Enterococcus faecalis, Enterococcus faecium, or Staphylococcus aureus had a significant impact on the proteome of C. jejuni, with S. aureus having the highest influence. The study also found a shared proteomic response and synergistic proteomic response when co-incubation and exposure to bile acid deoxycholate occurred together.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Luca Robinson, Janie Liaw, Zahra Omole, Dong Xia, Arnoud H. M. van Vliet, Nicolae Corcionivoschi, Abderrahman Hachani, Ozan Gundogdu
Summary: The Type VI Secretion System (T6SS) plays crucial roles in bacterial antagonism, subversion of host cells, and niche colonisation. A unique and conserved T6SS cluster associated with Campylobacter jejuni Integrated Element 3 (CJIE3) was identified as Campylobacter jejuni Pathogenicity Island-1 (CJPI-1), which contains canonical VgrG homologues and a Type II toxin-antitoxin module.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Gayani Weerasooriya, Andrea R. McWhorter, Samiullah Khan, Kapil K. Chousalkar
Summary: Campylobacter is the most common bacterial cause of gastrointestinal disease, and contaminated poultry is often the source of infection. This study examines the survivability and virulence potential of sublethally injured Campylobacter in mice, following exposure to commonly used chemicals in poultry meat processing. The results show that sublethally injured Campylobacter jejuni has reduced virulence and colonization potential in mice.
MICROBIOLOGY SPECTRUM
(2022)
Review
Microbiology
Walid Ghazi Al Hakeem, Shahna Fathima, Revathi Shanmugasundaram, Ramesh K. Selvaraj
Summary: C. jejuni is the leading cause of human foodborne illness associated with poultry, beef, and pork consumption. Horizontal transmission from the environment is considered to be the primary source of C. jejuni in commercial poultry farms. C. jejuni expresses virulence factors regulated by a two-component system that mediates its ability to survive in the host.
Review
Biochemistry & Molecular Biology
Amber D. Gabbert, Jennifer L. Mydosh, Prabhat K. Talukdar, Lisa M. Gloss, Jason E. McDermott, Kerry K. Cooper, Geremy C. Clair, Michael E. Konkel
Summary: Campylobacter jejuni is the most common bacterial cause of gastroenteritis worldwide, causing millions of cases of inflammatory diarrhea. It is associated with post-infection complications in developed countries and malnutrition and growth-stunting in low- and middle-income countries. Our understanding of C. jejuni pathogenesis is incomplete, but we focus on the secretion systems and their role in host-cell interactions and disease establishment. The flagellar type III secretion system and putative effectors are of particular interest.
Article
Biochemistry & Molecular Biology
Harald Nothaft, Xiaoming Bian, Asif Shajahan, William G. Miller, David T. Bolick, Richard L. Guerrant, Parastoo Azadi, Kenneth K. S. Ng, Christine M. Szymanski
Summary: The study identified C. jejuni isolates expressing a hexasaccharide lacking the glucose branch, caused by a missing aspartate in the DXDD motif of the glucosyltransferase PglI. Mouse vaccination with E. coli expressing the full-length heptasaccharide demonstrated reduction in C. jejuni colonization and weight loss. The study suggests the importance of considering glucose inclusion in N-glycan-based vaccines for preventing campylobacteriosis.
ACS CHEMICAL BIOLOGY
(2021)
Article
Microbiology
Basanta R. Wagle, Annie M. Donoghue, Palmy R. Jesudhasan
Summary: The study demonstrates that allyl sulfide and ginger oil can significantly reduce Campylobacter in poultry meat, while curcumin and other phytochemicals also reduce adhesion and quorum sensing of the bacteria, leading to cell membrane damage or death.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Mohamad Mortada, Douglas E. Cosby, Gabriel Akerele, Nour Ramadan, Jarred Oxford, Revathi Shanmugasundaram, Theros T. Ng, Ramesh K. Selvaraj
Summary: This study investigated the immune response of chickens to Campylobacter jejuni colonization, revealing that challenged birds showed persistent colonization in the ceca with subsequent dissemination to the spleen and liver. The increase in anti-Campylobacter antibodies in the serum and bile, suppression of T-lymphocytes, and changes in mRNA levels and cytokine production indicate a complex immune response. The balanced Th1 and Th2 immune responses against C. jejuni may play a role in bacterial colonization and the lack of pathology in challenged birds.
Article
Microbiology
Jinshil Kim, Haejoon Park, Sangryeol Ryu, Byeonghwa Jeon
Summary: This study successfully controlled antimicrobial-resistant E. coli on retail chicken using a phage cocktail, which demonstrated effective infection against various phylogenetic groups of resistant strains and maintained inhibition at refrigeration temperatures. The phage cocktail reduced viable counts of AMR E. coli by approximately 3 log units within 3 hours on contaminated chicken skin, without developing resistance throughout the experiment.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Myungseo Park, Sunyoung Hwang, Sangryeol Ryu, Byeonghwa Jeon
Summary: The study revealed that iron upregulates cosR transcription and subsequently positively regulates perR transcription in C. jejuni's defense against oxidative stress. This research provides insights into how C. jejuni coordinates oxidative stress defense by regulating the transcription of perR and cosR in response to iron.
Editorial Material
Microbiology
Byeonghwa Jeon, Taradon Luangtongkum, Zhangqi Shen, Catherine M. Logue, Jun Lin
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jinshil Kim, Jeong In Hur, Sangryeol Ryu, Byeonghwa Jeon
Summary: This study aimed to improve Campylobacter isolation by inhibiting the growth of ESBL-producing E. coli using bacteriophages. Supplementing Campylobacter-selective media with E. coli phages reduced the level of ESBL-producing E. coli during enrichment. Antimicrobial synergy was observed when E. coli phages were combined with the antimicrobial supplements in Campylobacter-selective media, especially with rifampicin.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
Migma Dorji Tamang, Junghee Bae, Myungseo Park, Byeonghwa Jeon
Summary: This study found that the antioxidant octyl gallate (OG) enhanced the antibacterial activity of antibiotics against methicillin-resistant Staphylococcus aureus (MRSA), making MRSA more susceptible to beta-lactams.
Article
Food Science & Technology
Hyeeun Park, Jinshil Kim, Sangryeol Ryu, Byeonghwa Jeon
Summary: PMQR genes are frequently detected in ESBL-producing E. coli isolates from retail raw chicken in South Korea, posing a public health concern.
FOOD SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Immunology
Byeonghwa Jeon, Tunchanok Saisom, Jiroj Sasipreeyajan, Taradon Luangtongkum
Summary: Controlling Campylobacter in poultry before harvest is crucial for reducing foodborne infections. This study evaluated the efficacy of pre-colonization with oxidative stress defense mutants in preventing Campylobacter colonization in chickens. The results showed that the ahpC and katA mutants significantly reduced C. jejuni colonization.
Article
Microbiology
Myungseo Park, Liz Horn, Victoria Lappi, Dave Boxrud, Craig Hedberg, Byeonghwa Jeon
Summary: This study found that licorice extract combined with aminoglycosides has significant antimicrobial synergy in L. monocytogenes, reducing the minimum inhibitory concentration of gentamicin and making gentamicin-resistant clinical isolates of L. monocytogenes susceptible to gentamicin.
Article
Microbiology
Myungseo Park, Jinshil Kim, Liz Horn, Jisun Haan, Ali Strickland, Victoria Lappi, David Boxrud, Craig Hedberg, Sangryeol Ryu, Byeonghwa Jeon
Summary: The level of biofilm production in L. monocytogenes varies among different serotypes and is associated with phylogenetic lineage. The sugar decoration of bacterial cell walls with l-rhamnose is responsible for strong biofilm production in serotypes 1/2a and 1/2b. This study enhances our understanding of the relationship between biofilm production, phylogenetic lineage, and serotype in L. monocytogenes.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Myungseo Park, Jinshil Kim, Jill Feinstein, Kevin S. Lang, Sangryeol Ryu, Byeonghwa Jeon
Summary: Antibiotic tolerance in C. jejuni leads to the development of antibiotic resistance, particularly fluoroquinolone resistance. Exposure to ciprofloxacin or tetracycline induces antibiotic tolerance and promotes the emergence of fluoroquinolone-resistant C. jejuni clones. Alkyl hydroperoxide reductase plays a critical role in reducing the development of fluoroquinolone resistance after tolerance induction.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Jeong In Hur, Jinshil Kim, Sangryeol Ryu, Byeonghwa Jeon
Summary: This study demonstrates the existence of phylogenetic clusters in Campylobacter jejuni associated with cold stress tolerance and identifies the genetic element cfrA as a contributor to cold stress tolerance.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Pitchaya Santativongchai, Phitsanu Tulayakul, Yinduo Ji, Byeonghwa Jeon
Summary: This study found that the combination of octyl gallate (OG) with penicillin and bacitracin can significantly enhance the antimicrobial and antibiofilm activities against S. epidermidis. It lowers the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), and prevents biofilm formation. In addition, OG improves the permeability of the bacterial cell wall, thereby enhancing its antimicrobial synergy with penicillin and bacitracin.
Article
Microbiology
Jinshil Kim, Myungseo Park, Eunbyeol Ahn, Qingqing Mao, Chi Chen, Sangryeol Ryu, Byeonghwa Jeon
Summary: The ability of a foodborne pathogen to tolerate environmental stress is crucial for food safety. Campylobacter jejuni, a leading cause of foodborne illnesses, has been found to develop a thick layer of bacterial capsules to protect itself under aerobic conditions.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Pitchaya Santativongchai, Phitsanu Tulayakul, Byeonghwa Jeon
Summary: This study aims to develop a synergistic approach to better control Listeria biofilms using nisin, in combination with gallic-acid-rich food plant extracts. The results showed that gallic acid significantly decreased the level of biofilm formation in L. monocytogenes, while ethyl gallate, propyl gallate, and lauryl gallate enhanced biofilm production. Sage extracts enhanced the antibiofilm activity of nisin against L. monocytogenes, but the other extracts increased biofilm formation.
Article
Environmental Sciences
Chalalai Rueanghiran, Atchara Dawanpa, Nayika Pinneum, Arsooth Sanguankiat, Chart Chiemchaisri, Wilai Chiemchaisri, Watcharapong Sritumpawa, Olarn Kijpreedaborisuthi, Byeonghwa Jeon, Phitsanu Tulayakul
Summary: The study investigated the occurrence of antimicrobial drug resistance in the swine production industry, focusing on phenotypic and genotypic resistance characteristics of E. coli and antibiotic residues in manure and wastewater. The results showed that farmers commonly used antibiotics for disease treatment and prevention, and there were high levels of antibiotic residues in wastewater. The study also examined the minimum inhibitory concentrations and detected multidrug resistance in E. coli isolates. Genetic analysis revealed the presence of diverse antimicrobial resistance genes in animal manure and wastewater.
EMERGING CONTAMINANTS
(2022)