4.4 Article

Metabolic precursors and cofactors stimulate astaxanthin production in Paracoccus MBIC 01143

期刊

FOOD SCIENCE AND BIOTECHNOLOGY
卷 21, 期 6, 页码 1695-1700

出版社

KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-012-0225-8

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astaxanthin; Paracoccus MBIC 01143; malate; ferrous sulphate

资金

  1. University Grants Commission, India

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This work investigates bacterial production of astaxanthin as an alternative to production by algae and yeast owing to its lower incubation time and simpler downstream processing. The physical parameters and medium composition were optimized for astaxanthin production by Paracoccus MBIC 01143. The optimized media was supplemented with tricarboxylic acid intermediates to enhance the pool of precursors, while the cofactors of Crt enzymes (ferrous sulphate, ascorbate, NADPH, ATP, and 2-oxoglutarate) were added to stimulate their enzyme activity aiming at higher astaxanthin accumulation. Malate at 5 mM and ferrous sulphate at 1 mM increased the astaxanthin production from 177 to 3,750 mu g/L.

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