期刊
FOOD SCIENCE AND BIOTECHNOLOGY
卷 20, 期 6, 页码 1633-1638出版社
SPRINGER
DOI: 10.1007/s10068-011-0225-0
关键词
denaturing gradient gel electrophoresis (DGGE); microbial diversity; microorganisms; douchi
资金
- National Basic Research Program of China [2005DKA21202]
- National Natural Science Foundation of China [NSF30860008, 31000048, 30900038, 31170091, 81160494]
- Training Plan for the Main Subject of Academic Leaders of Jiangxi Province
To track changes in dynamic microbial communities during post-fermentation process, traditional culture method, and denaturing gradient gel electrophoresis (DGGE) were used to study the number and species of dominant microorganisms in douchi. The result of culturedependent method showed that the microbial number changed slightly since the 4(th) day while the DGGE indicated that the really steady-state was achieved from the 10(th) day. In addition, Lactococcus lactis subsp., Staphylococcus lentus, and 2 uncultured bacterium were identified to occupy the dominant positions in bacterial DGGE pattern, and Bacillus thermoamylovorans, Bacillus subtilis, Enterobacter spp., and Absidia corymbifera, Pichia guilliermondii, Pichia farinose were also detected from Bacillus and fungal DGGE patterns, respectively. In conclusion, some pathogenic microorganisms involving in the douchi fermentation had been detected throughout the post-fermentation process, and the combination of culturedependent and -independent method was proved to be effective in profiling microbial diversity.
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