期刊
FOOD RESEARCH INTERNATIONAL
卷 116, 期 -, 页码 1153-1162出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2018.09.061
关键词
Lupinus angustifolius; Australian sweet lupin; Seed coat; Polyphenols; Genotype by environment; By-product; Hplc-DAD-ESI-MS/MS
资金
- Curtin International Postgraduate Research Scholarship (CIPRS)/Health Sciences Faculty International Research Scholarship (HSFIRS)
- National Health and Medical Research Council of Australia Senior Research Fellowship [1116973]
- Royal Perth Hospital Medical Research Foundation Fellowship
- Western Australian State Government
- Australian Federal Government through Bioplatforms Australia
- National Collaborative Research Infrastructure Strategy (NCRIS)
Seeds of the legume lupin (Lupinus spp.) are becoming increasingly important as human food. The seed coat, at similar to 25% of the whole seed of Lupinus angustifolius (Australian sweet lupin, ASL), is the main by-product of lupin kernel flour production. The primary market for lupin seed coat is low value feed with very limited use in foods. In this study, seed coats of six ASL commercial varieties from two growing sites were sampled for identification and quantification of polyphenols using a high-performance liquid chromatography (HPLC) with diode array detector (DAD) and coupled with a triple quadrupole mass spectrometer which equipped with electrospray ionization source (ESI MS/MS). Three flavones (apigenin-7-O-beta-apiofuranosyl-6,8-di-C-beta-glucopyranoside, vicenin 2, and apigenin-7-O-beta-glucopyranoside), one isoflavone (genistein) and one dihydroflavonol derivative (aromadendrin-6-C-beta-D-glucopyranosyl-7-O-[beta-D-apiofuranosyl-(1 -> 2)]-O-beta-D-glucopyranoside), and several hydroxybenzoic and hydroxycinnamic acid derivatives were identified. Considerable variations in levels of individual polyphenols were found but apigenin-7-O-beta-apiofuranosyl-6,8-di-C-beta-glucopyranoside was the predominant polyphenol in all samples accounting for 73.08-82.89% of the total free polyphenols. These results suggest that ASL seed coat could be valuable dietary source of polyphenols.
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