期刊
FOOD RESEARCH INTERNATIONAL
卷 48, 期 1, 页码 265-270出版社
ELSEVIER
DOI: 10.1016/j.foodres.2012.05.005
关键词
Plant sterols; Phytosterol oxidation products; Gas chromatography; Phytosterol stability; Sterol oxidation; Functional foods
资金
- CICYT-FEDER [AGL2008-02591-C02-01]
- Consolider Fun-C-Food [CSD2007-00063]
- Generalitat Valenciana (Spain) [GVACOMP2011-195]
- Instituto Danone (Spain)
Two different plant sterol (PS) sources (free PS from tall oil and esterified PS from vegetable oils) were used for manufacturing two types of functional beverages (fruit and milk-based fruit beverages), and their PS and phytosterol oxidation product (POP) contents were determined. Gas chromatography-tandem mass spectrometry (GC-MS/MS) was used for identification and gas chromatography-flame ionization detection (GC-FID) for quantitation purposes. Brassicasterol, campesterol, campestanol, stigmasterol, beta-sitosterol and sitostanol were the quantified PS, conforming a profile in order with current legislation. The relative percentages of PS differed according to the enrichment source involved, though the enrichment levels (g/100 g beverage) were of the same order (1.77 from tall oil and 1.84 from vegetable oils). Only POPs from beta-sitosterol (the prevalent PS in the analyzed beverages) were detected the predominant representative being 7 beta-hydroxysitosterol (39-58.5% of total POP content). The following POPs were quantified: 7 alpha-hydroxy, beta-epoxy, alpha-epoxy, and 7-ketositosterol, yielding a total POP content ranging between 42.9 and 57.4 mg/100 g of PS. No statistically significant differences (p > 0.05) in total and individual POP content according to the source of PS were found. The mean beta-sitosterol oxidation percentage was <0.07%, which reflected a low PS oxidation extent, though manufacture was on a laboratory scale regardless of the PS source used in enrichment of the functional beverages. These functional drinks therefore can be regarded as healthy food products and as an adequate PS vehicle as well. (C) 2012 Elsevier Ltd. All rights reserved.
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