期刊
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
卷 96, 期 9, 页码 3233-3239出版社
WILEY
DOI: 10.1002/jsfa.7506
关键词
grass carp fillets; texture; softening; cathepsin activity; microorganism; ice storage
资金
- National Natural Science Foundation of China [NSF31301508]
- China Agriculture Research System [CARS-46]
- Natural Science Foundation of Jiangsu Province [BK20130138]
- College Graduate Research and Innovation Project of Jiangsu Province [KYLX_1171]
BACKGROUND: Texture deterioration often negatively affects sensory attributes and commercial values of ice-stored fish fillets. The mechanism of softening of fish fillets during chilling storage is not fully resolved. Grass carp is a predominant freshwater fish species in China. The objective of the present study was to investigate the differential role of endogenous cathepsin and microorganisms in texture softening of ice-stored grass carp fillets. RESULTS: The fillets were immersed in either NaN3 solution to reducemicrobial activity or in iodoacetic acid solution to exclude cathepsin activity before ice storage. Treatment with NaN3 reduced microbial load of fillets below 2 log CFU g-1 muscle during the entire storage period, and had no significant influence on the cathepsin activity and proteolysis. But the shear force of fillets treatedwithNaN(3) decreased by66% after 21 days of storage. Meanwhile, treatment with iodoacetic acid inactivated cathepsin B and B+ L but did not significantly affect themicrobial growth of fillets. Compared to NaN3 treatment, iodoacetic acid effectively alleviated softening and inhibited the increase in TCA-soluble peptides during storage. CONCLUSION: This study demonstrated that proteolysis induced by endogenous cathepsins, rather thanmicroorganisms, plays an important role in texture softening of ice-stored grass carp fillets. (C) 2015 Society of Chemical Industry
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