期刊
FOOD CHEMISTRY
卷 152, 期 -, 页码 386-390出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.11.080
关键词
Cyanidin 3-glucoside; Blue honeysuckle fruits; High-speed counter-current; chromatography (HSCCC)
资金
- Special Fund for Agro-scientific Research in the Public Interest [201103037]
- Funding Project of Competence Development Program for Beijing VET Teachers
- Scientific Research Base Fund for Science and Technology Innovation and Transformation Program, PHR (IHLB), Beijing Famous Teacher Program
- Science-Technology Foundation of Beijing Municipal Commission of Education [KM201410858002]
- Youth elite project of Higher Education of Beijing [YETP1801]
Blue honeysuckle fruits are rich in anthocyanins with many beneficial effects such as reduction of the risk of cardiovascular diseases, diabetes and cancers. High-speed counter-current chromatography (HSCCC) was used for the separation of anthocyanin on a preparative scale from blue honeysuckle fruit crude extract with a biphasic solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/ water/trifluoroacetic acid (2:2:1:5:0.01, v/v) for the first time in this paper. Each injection of 100 mg crude extract yielded 22.8 mg of cyanidin 3-glucoside (C3G) at 98.1% purity. The compound was identified by means of electro-spray ionisation mass (ESI/MS) and 1H and 13C nuclear magnetic resonance (NMR) spectra.(C) 2013 Elsevier Ltd. All rights reserved.
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