Article
Electrochemistry
Kavita Jayakumar, Thomas M. B. Reichhart, Christopher Schulz, Roland Ludwig, Alfons K. G. Felice, Donal Leech
Summary: Cellobiose dehydrogenase (CDH) is a biocatalyst capable of oxidizing cellobiose and related carbohydrates to generate electrical current. A novel engineered CDH has shown improved performance as a bioelectrocatalyst in glucose biosensors.
Article
Biochemistry & Molecular Biology
Sudarma Dita Wijayanti, Franziska Schachinger, Roland Ludwig, Dietmar Haltrich
Summary: We investigated the bioelectrochemical properties of an FAD-dependent glucose dehydrogenase from Trichoderma virens (TvGDH) and its behavior when immobilized on a graphite electrode. TvGDH was found to prefer maltose over glucose as substrate, making it suitable for a maltose sensor. By entrapping TvGDH in an osmium redox polymer, we were able to create a biosensor with a sensitivity of 1.7 μA mM-1cm-2, a linear range of 0.5-15 mM, and a detection limit of 0.45 mM towards maltose.
BIOELECTROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Won-Yong Jeon, Hyug-Han Kim, Young-Bong Choi
Summary: The study focuses on using PDA-MWCNTs to effectively attach Ru(dmo-bpy)(2)Cl-2 and GDH onto SPCEs for glucose detection without needing a cross-linker. Experimental results show a wide linear range of glucose-concentration-dependent responses and the MPS technique facilitates selective detection of glucose. The ease of device fabrication and high detection performance make this a promising pathway for developing glucose sensors based on the GDH enzyme and Ru(dmo-bpy)(2)Cl-2 redox mediator.
Article
Biophysics
Haiyan Song, Xigui Zhou, Zhiguang Zhu
Summary: The study demonstrates an integrated biosensor device for analyzing xylose fermentation samples. Real sample testing shows that the biosensor has high selectivity, recovery, reproducibility, and long-term stability.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Motaher M. Hossain, Jannatul Morshed, Seiya Tsujimura
Summary: A bio-conjugated redox network matrix has been developed on a glassy carbon electrode through covalent bonding with one-pot crosslinking, using glucose dehydrogenase, thionine, and poly(ethylene glycol) diglycidyl ether as components. This matrix allows for a glucose oxidation current of 400 mu A cm(-2) at 25 degrees C, with electrons from the enzyme diffusing through the network.
CHEMICAL COMMUNICATIONS
(2021)
Review
Food Science & Technology
Mingru Yao, Feifei Sun, Jiyun Nie, Qing-Li Yang, Wei Wu, Fangyuan Zhao
Summary: This paper reviews the research progress of electrospinning in food detection and introduces the characteristics of electrospinning materials and their application with biosensing platforms. The sensitivity and detection limit of various methods and materials in detection are also mentioned, as well as the challenges faced in future exploration.
FOOD REVIEWS INTERNATIONAL
(2023)
Review
Biochemistry & Molecular Biology
Iruthayapandi Selestin Raja, Mohan Vedhanayagam, Desingh Raj Preeth, Chuntae Kim, Jong Hun Lee, Dong Wook Han
Summary: Food safety issues have led to a need for rapid detection of food contaminants, with electrochemical biosensors based on 2D nanomaterials showing potential to enhance sensor sensitivity and stability, which has attracted researchers' attention.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Paulina K. Wells, Artem Melman, Evgeny Katz, Oleh Smutok
Summary: A new nano-structured platform using PQQ-dependent glucose dehydrogenase was developed for fluorescent analysis. The platform immobilized the enzyme on SiO2 nanoparticles and used a smartphone for signal quantification. This platform shows great potential for developing a variety of biosensors for different analytes.
Review
Food Science & Technology
Nevetha Ravindran, Sandhya Kumar, M. Yashini, S. Rajeshwari, C. A. Mamathi, Nirmal S. Thirunavookarasu, C. K. Sunil
Summary: Food safety is a crucial concern for building trust, and advancements in technology have made it easier to ensure safety in various aspects. Biosensors, such as SPR, offer fast, accurate, and cost-effective detection in food analysis, allowing for the identification of contaminants and additives.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Food Science & Technology
Sudarma Dita Wijayanti, Lidiia Tsvik, Dietmar Haltrich
Summary: Food analysis and control are crucial for ensuring the quality and safety of food products. Electrochemical biosensors based on enzymes offer high selectivity, sensitivity, short analysis time, and cost-effectiveness, making them promising tools for food analysis.
Article
Chemistry, Analytical
Ningning Wang, Xuewei Cao, Daxi Sun, Xinyu Li, Geng Tian, Jiankai Feng, Pengfei Wei
Summary: A polymer dots (Pdots)-based electrochemiluminescence (ECL) biosensor was developed for the detection of NADH and three metabolites. The biosensor exhibited wide detection ranges, high selectivity, and low limits of detection for glucose, lactic acid, and pyruvate. This biosensor has the potential to be a powerful tool for point-of-care use and disease monitoring.
Article
Chemistry, Multidisciplinary
Gheorghe Nechifor, Florentina Mihaela Pancescu, Alexandra Raluca Grosu, Paul Constantin Albu, Ovidiu Oprea, Szidonia-Katalin Tanczos, Constantin Bungau, Vlad-Alexandru Grosu, Andreia Pirtac, Aurelia Cristina Nechifor
Summary: Composite membranes play a crucial role in separation, concentration, and purification processes, especially in membrane reactors and membrane bioreactors. The development of composite membranes, incorporating various nanoparticles, polymeric, oxide, or metal materials, has shown promising potential in enhancing reactivity and selectivity. The preparation and characterization of an active metal nanoparticle-support polymer type composite membrane, specifically using osmium nanoparticles on a polypropylene hollow fiber membrane, demonstrated successful results in the redox process of target substances in solutions of lower aliphatic alcohols.
Review
Chemistry, Analytical
G. Ozcelikay, S. Kaya, E. Ozkan, A. Cetinkaya, E. Nemutlu, S. Kir, S. A. Ozkan
Summary: Metabolomics, as a technology for identifying and analyzing metabolites, has gained broad interest and is widely used for biomarker discoveries due to its high throughput results. However, the application of metabolomics requires advanced analytical methods. The use of molecularly imprinted polymer (MIP)-based electro sensors, which have high selectivity, sensitivity, easy applicability, portability, and low cost, can overcome the limitations of complex and limited availability of instruments. These sensors have great potential in targeted metabolomics analysis and the development of point-of-care tests (POCT) for easy patient application.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xin-Yao Yu, Jun-Yi He, Fei Tang, Peng Yu, Ling Wu, Zhong-Liang Xiao, Li-Xian Sun, Zhong Cao, Donghong Yu
Summary: In this work, a sensor based on molecularly imprinted polymers (MIPs) for l-glutamic acid (l-Glu) was developed through electrochemical polymerization. The optimized reaction conditions resulted in an electrode with excellent catalytic oxidation activity, allowing for the rapid and accurate detection of l-Glu at low concentrations.
ANALYTICAL METHODS
(2023)
Article
Biochemistry & Molecular Biology
D. V. Estrada-Osorio, Ricardo A. Escalona-Villalpando, L. G. Arriaga, A. Gutierrez, J. Ledesma-Garcia
Summary: Ferrocene-based polymers have been widely studied as redox mediators in glucose biosensors. This work successfully modified poly-L-lysine (PLL) with ferrocene carboxylate and tested its performance as a redox mediator with glucose oxidase. The results showed remarkable activity and high selectivity. Scanning electrochemical microscopy was used to analyze the charged regions of the modified polymer on the electrode surface.
BIOELECTROCHEMISTRY
(2022)
Article
Chemistry, Physical
Davide Piccinino, Michela Delfino, Giorgia Botta, Marcello Crucianelli, Valentina Grossi, Maurizio Passacantando, Riccarda Antiochia, Gabriele Favero, Raffaele Saladino
APPLIED CATALYSIS A-GENERAL
(2015)
Article
Biophysics
Cristina Tortolini, Paolo Bollella, Marta Letizia Antonelli, Riccarda Antiochia, Franco Mazzei, Gabriele Favero
BIOSENSORS & BIOELECTRONICS
(2015)
Article
Chemistry, Analytical
Marilena Carbone, Lo Gorton, Riccarda Antiochia
Article
Chemistry, Analytical
Paolo Bollella, Lo Gorton, Roland Ludwig, Riccarda Antiochia
Article
Chemistry, Multidisciplinary
Eliana Capecchi, Davide Piccinino, Ines Delfino, Paolo Bollella, Riccarda Antiochia, Raffaele Saladino
Article
Chemistry, Multidisciplinary
Federico Tasca, Riccarda Antiochia
Article
Chemistry, Multidisciplinary
Cristina Tortolini, Eliana Capecchi, Federico Tasca, Riccardo Pofi, Mary Anna Venneri, Raffaele Saladino, Riccarda Antiochia
Summary: Nanocomposites based on lignin nanoparticles were designed for electrochemical biosensing, showing strong inhibition effect on electron transfer reaction. The modified electrodes assembled with biosensors successfully demonstrated sensitivity towards glucose, with the three-layered structure exhibiting the best analytical performance.
Review
Chemistry, Analytical
Riccarda Antiochia
Summary: This review summarizes the current state of paper-based biosensors (PBBs) used in the detection of COVID-19, specifically focusing on three categories for SARS-CoV-2 diagnostics. It highlights the characteristics, analytical performance, advantages and drawbacks of each type of biosensor compared to traditional methods, with the hope of assisting scientists in developing novel PBB platforms with enhanced performance to help contain the COVID-19 outbreak.
Article
Chemistry, Analytical
Cristina Tortolini, Federico Tasca, Mary Anna Venneri, Cinzia Marchese, Riccarda Antiochia
Summary: The study investigated the effects of nanostructured modifications of a gold electrode surface on the development of electrochemical sensors for L-ascorbic acid detection. By modifying a gold electrode with gold single-walled carbon nanotubes (Au/SWCNTs) and highly nanoporous gold (h-nPG) film, the sensors demonstrated enhanced sensitivity and lower detection limits compared to a bare gold electrode. The h-nPG electrode, in particular, was successfully used for ascorbic acid detection in human urine with satisfactory recovery levels and minimal interference.
Article
Biochemistry & Molecular Biology
Valeria Gigli, Davide Piccinino, Daniele Avitabile, Riccarda Antiochia, Eliana Capecchi, Raffaele Saladino
Summary: The overproduction of eumelanin can result in unaesthetic hyper-pigmented skin diseases. The current treatment methods, such as tyrosinase inhibitors, have harmful side effects. This study found that using laccase from Trametes versicolor and natural phenol redox mediators can efficiently degrade eumelanin, providing an alternative to traditional whitening agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Valeria Gigli, Cristina Tortolini, Eliana Capecchi, Antonio Angeloni, Andrea Lenzi, Riccarda Antiochia
Summary: In this study, a reliable biosensor was developed using chitosan nanoparticles and tyrosinase for accurate detection of total catecholamine levels in human urine samples. Through optimization of loading methods and enzyme concentration, the biosensor exhibited good stability and sensitivity.
Article
Chemistry, Analytical
Cristina Tortolini, Valeria Gigli, Antonio Angeloni, Luciano Galantini, Federico Tasca, Riccarda Antiochia
Summary: In this work, a simple electrochemical immunosensor was developed for the detection of D-dimer protein in human plasma samples. The immunosensor was constructed by drop-casting chitosan nanoparticles (CSNPs) as a biocompatible support, Protein A (PrA) to facilitate antibody orientation, and D-dimer antibody onto a carboxyl functionalized multi-walled carbon nanotubes screen printed electrode (MWCNTs-SPE). The immunosensor showed promising performance in antibody immobilization and specific D-dimer detection, with a linear range from 2 to 500 μg L-1, LOD of 0.6 μg L-1, and sensitivity of 1.3 μA L μg(-1) cm(-2). The stability and fast response time (5 s) were also reported. Moreover, the immunosensor demonstrated satisfactory results in detecting D-dimer in human plasma samples.
Article
Chemistry, Analytical
Cristina Tortolini, Valeria Gigli, Flavio Rizzo, Andrea Lenzi, Mariano Bizzarri, Antonio Angeloni, Riccarda Antiochia
Summary: This paper describes the development of a simple voltammetric biosensor for the stereoselective discrimination of myo-inositol and D-chiro-inositol by means of bovine serum albumin adsorption onto a multi-walled carbon nanotube graphite screen-printed electrode.
Article
Chemistry, Analytical
Cristina Tortolini, Paolo Bollella, Rosaceleste Zumpano, Gabriele Favero, Franco Mazzei, Riccarda Antiochia
Article
Chemistry, Analytical
Mauro Tomassetti, Giorgia Conta, Luigi Campanella, Gabriele Favero, Gabriella Sanzo, Franco Mazzei, Riccarda Antiochia
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
